Asunto(s)
Aumento de la Cresta Alveolar/métodos , Tornillos Óseos/efectos adversos , Aleaciones Dentales/química , Implantación Dental Endoósea/métodos , Implantes Dentales , Electrogalvanismo Intrabucal , Dolor Facial/etiología , Complicaciones Posoperatorias/etiología , Prótesis Dental de Soporte Implantado , Fracaso de la Restauración Dental , Femenino , Humanos , Persona de Mediana Edad , Retratamiento , Titanio/químicaRESUMEN
The next generation of dental professionals will require an improved understanding of the interplay between oral health and general health. An aging population, a greater proportion of medically complex patients and an evolving health care delivery system all call for changes in the ways dentists are trained. A concerted effort to broaden primary health-care activities in the dental office will greatly improve both oral and general health outcomes. It is proposed that expansion of biomedical training in the predoctoral curriculum, mandated PGY-1 postgraduate training and improved continuing education courses would provide the basis for achieving these goals.
Asunto(s)
Educación en Odontología , Medicina Oral/educación , Curriculum , Educación Médica , Educación Médica Continua , Humanos , Internado y Residencia , Ciencia/educaciónRESUMEN
We report the identification and characterization of a novel lipid kinase that phosphorylates multiple substrates. This enzyme, which we term MuLK for multi-substrate lipid kinase, does not belong to a previously described lipid kinase family. MuLK has orthologs in many organisms and is broadly expressed in human tissues. Although predicted to be a soluble protein, MuLK co-fractionates with membranes and localizes to an internal membrane compartment. Recombinant MuLK phosphorylates diacylglycerol, ceramide, and 1-acylglycerol but not sphingosine. Although its affinity for diacylglycerol and ceramide are similar, MuLK exhibits a higher V(max) toward diacylglycerol in vitro, consistent with it acting primarily as a diacylglycerol kinase. MuLK activity was inhibited by sphingosine and enhanced by cardiolipin. It was stimulated by calcium when magnesium concentrations were low and inhibited by calcium when magnesium concentrations were high. The effects of charged lipids and cations on MuLK activity in vitro suggest that its activity in vivo is tightly regulated by cellular conditions.
Asunto(s)
Lípidos/química , Fosfotransferasas (Aceptor de Grupo Alcohol)/química , Fosfotransferasas (Aceptor de Grupo Alcohol)/genética , Secuencia de Aminoácidos , Animales , Encéfalo/metabolismo , Cardiolipinas/química , Cationes , Membrana Celular/metabolismo , ADN Complementario/metabolismo , Diacilglicerol Quinasa/química , Diglicéridos/química , Relación Dosis-Respuesta a Droga , Biblioteca de Genes , Genoma Humano , Proteínas Fluorescentes Verdes , Humanos , Iones , Cinética , Proteínas Luminiscentes/metabolismo , Ratones , Datos de Secuencia Molecular , Páncreas/metabolismo , Fosforilación , Filogenia , Estructura Terciaria de Proteína , ARN Mensajero/metabolismo , Proteínas Recombinantes/química , Esfingosina/metabolismo , Fracciones Subcelulares/metabolismo , Distribución TisularRESUMEN
In December 1997, 170 hemorrhagic fever-associated deaths were reported in Garissa District, Kenya. Laboratory testing identified evidence of acute Rift Valley fever virus (RVFV). Of the 171 persons enrolled in a cross-sectional study, 31(18%) were anti-RVFV immunoglobulin (Ig) M positive. An age-adjusted IgM antibody prevalence of 14% was estimated for the district. We estimate approximately 27,500 infections occurred in Garissa District, making this the largest recorded outbreak of RVFV in East Africa. In multivariable analysis, contact with sheep body fluids and sheltering livestock in one s home were significantly associated with infection. Direct contact with animals, particularly contact with sheep body fluids, was the most important modifiable risk factor for RVFV infection. Public education during epizootics may reduce human illness and deaths associated with future outbreaks.