Physical Characteristics and Microbial Quality of Ostrich Meat in Relation to the Type of Packaging and Refrigerator Storage Time.

The aim of this study was to evaluate the effect of the packaging system type on the physical characteristics and microbial changes in ostrich meat during refrigerated storage. The applied packaging systems were vacuum packaging (VP) and modified atmosphere packaging (MAP) using two combinations of gases: MAP1 (40% O2/40% CO2/20% N2) and MAP2 (60% O2/30% CO2/10% N2). Eight meat samples were obtained in three replicates for all parameters, except for pH, for which six replicates were obtained from the M. ilifibularis (IF) muscle, and were stored in a refrigerator at 2 °C and analyzed at 0, 4, 8, 12 and 16 days for the effect of packaging methods on physical meat quality. The initial pH (5.99) decreased at the end of the storage time for MAP1 to 5.81, whereas VP was stable from day 0 to 12 and increased up to 6.08 on day 16. Regarding meat color, the L* value increased during storage for MAP1 and MAP2 from 36.99 to 40.75 and 41.60, respectively, whereas it declined for VP to 34.22. The same tendencies were reported for redness (a*) and yellowness (b*). Drip loss was the lowest in MAP1 and highest in VP. The lowest total viable bacteria counts were identified in VP, as compared to MAP1 and MAP2.

Changes in the health and behaviour of pet dogs during the COVID-19 pandemic as reported by the owners.

The COVID-19 pandemic affects human health, movement and behaviour, and this may consequently influence the behaviour and health of their pets. The aim of this study was to assess the impact of the COVID-19 pandemic on pet dogs' behaviour, as reported by their owners, as well as the owners' experience in relation to their dogs. We hypothesized that dog owners who underwent lockdown or quarantine would indicate more behavioural changes in their dogs and experience support in having a dog during the pandemic. An international online survey asked dog owners questions regarding their household, the dog, and dog-related changes during the pandemic. A total of 688 surveys, collected May-June 2020, were analysed. Respondents were from across Europe (87 %), the Americas (9%), and Asia and Australia (together 4%). Data were analysed in GLMM models with a binary distribution and country included as random variable. The main predictor variable was whether the respondent experienced lockdown (300 respondents, 44 %), quarantine (76 respondents, 11 %) or no restrictions (312 respondents, 45 %). Respondents who underwent lockdown or quarantine were 1.8 times more likely to report behavioural changes in their dogs (p = 0.02), with more negative behavioural changes in the dogs reported by respondents in lockdown than expected by chance. However, overall behavioural changes were more often positive (30 %) than negative (24 %). Respondents in lockdown were 2.6 times more likely to report health changes in their dogs (p = 0.02). The dog was perceived as a source of support during the pandemic: 65 % of the respondents indicated reduced tension due to their dog and 47 % indicated that the ability to walk the dog outside was another benefit. Advantages were reported more by respondents in lockdown and quarantine as compared to respondents who did not face these restrictions (p < 0.001). Difficulties in dog care were increased for respondents who experienced lockdown or quarantine (p < 0.01) and those who had no garden as compared to those who did (p < 0.001). One-third of the respondents took dog-related measures to prevent the spread of the coronavirus, and this was associated with more behavioural changes and more difficulties. In conclusion, lockdown, and to a lesser extent quarantine, may influence the dogs' behaviour and health, or the owners' awareness of it, and can contribute to a perceived tension-reduction in the owners.

The Composition of Fatty Acids in Ostrich Meat Influenced by the Type of Packaging and Refrigerated Storage.

Ostrich meat is a high-quality dietetic product, however, it is very sensitive to deterioration during storage. The aim of this study was to assess the effect of packaging systems on the fatty acid (FA) profiles in ostrich meat during refrigerated storage. The systems were: Vacuum packaging (VP) and modified atmosphere packaging (MAP) in two combinations of gases: MAP1 (40% O2/40% CO2/20% N2) and MAP2 (60% O2/30% CO2/10% N2). Samples were taken from the M. ilifibularis (IF) muscles of eight ostriches in each treatment group. The packs were stored in a refrigerator at 2 °C and analyzed at 0, 4, 8, 12 and 16 days. The packaging conditions and storage time had an impact on the concentration of bioactive compounds such as polyunsaturated fatty acids (PUFA), including n-3 such as C18:3, C20:5 (EPA) and C22:6 (DHA). The least changes in composition of n-3 and the sum of PUFA were recorded in ostrich meat packaged in vacuum, followed by that packaged using MAP1 and MAP2. The sum of n-6 PUFAs decreased significantly by 2.1% for MAP2, and only by 0.7% for vacuum packaging as the experiment progressed. A significant deterioration of these compounds was observed in all package systems, especially from day 12 until day 16 of storage.

Curcumin: Total-Scale Analysis of the Scientific Literature.

The current study aimed to provide a comprehensive bibliometric overview of the literature on curcumin, complementing the previous reviews and meta-analyses on its potential health benefits. Bibliometric data for the current analysis were extracted from the Web of Science Core Collection database, using the search string TOPIC=("curcumin*"), and analyzed by the VOSviewer software. The search yielded 18,036 manuscripts. The ratio of original articles to reviews was 10.4:1. More than half of the papers have been published since 2014. The major contributing countries were the United States, China, India, Japan, and South Korea. These publications were mainly published in journals representing the following scientific disciplines: biochemistry, chemistry, oncology, and pharmacology. There was a significant positive correlation between the total publication count and averaged citations per manuscript for affiliations, but not for countries/regions and journals. Chemicals that were frequently mentioned in the keywords of evaluated curcumin publications included curcuminoids, resveratrol, chitosan, flavonoids, quercetin, and polyphenols. The literature mainly focused on curcumin's effects against cancer, inflammation, and oxidative stress. Cancer types most frequently investigated were breast, colon, colorectal, pancreatic, and prostate cancers.

The Effect of Different Levels of Cu, Zn and Mn Nanoparticles in Hen Turkey Diet on the Activity of Aminopeptidases.

The aim of the study was to estimate the influence of the different levels of Cu, Zn, and Mn nanoparticles on the activity of aminopeptidases in turkey. An experiment was carried out on 144 turkey hen Hybrid Converter. The birds were divided into groups given standard- and nanoparticle-supplementation of different level of copper (Cu 20, 10, 2 mg/kg), zinc (Zn 100, 50, 10 ppm), and manganese (Mn 100, 50, 10 ppm), covering respectively 100%, 50%, and 10% of the physiological demands for those minerals in the diet. The activity of aminopeptidases (alanyl: AlaAP, leucyl: LeuAP and arginyl: ArgAP) after supplementation of minerals was determined in the breast and thigh turkey muscle. The strongest effect of interaction among minerals supplementation form and dose on the activity levels of the aminopeptidases in thigh muscle was observed for nano-Cu already at the lowest dose of 2 mg/kg. In this dose (covering 10% of the birds' demand) nano form of supplementation significantly increased the activity of Ala-, Leu-, and ArgAP (877, 201, and 719, respectively), compared to standard form of supplementation (461, 90.5, and 576, respectively). In turn, in breast muscle, after supplementation covering 10% of the demand with the nano-Cu, nano-Zn, and nano-Mn compared to the standard form, we did not observe any significant difference in the activity levels of any of the investigated aminopeptidases, except for AlaAP under Zn supplementation. Supplementation with the 20 mg/kg of Nano-Cu (100% of demand) and with 10 mg/kg of Nano-Cu (50% of demand) inhibited the activity of all of the three aminopeptidases in thigh muscle. Supplementation of the minerals in nano form into the diet, especially of Cu and Zn in the dose covering 10% of the demand is relevant to maintain homeostasis in turkey muscles, as indicated by the activity of the aminopeptidases.

Nutrients Composition in Fit Snacks Made from Ostrich, Beef and Chicken Dried Meat.

The aim of the study was to compare three types of meat snacks made from ostrich, beef, and chicken meat in relation to their nutrients content including fat, fatty acids, heme iron, and peptides, like anserine and carnosine, from which human health may potentially benefit. Dry meat samples were produced, from one type of muscle, obtained from ostrich (m.ambiens), beef (m. semimembranosus), and broiler chicken meat (m. pectoralis major). The composition of dried ostrich, beef, and chicken meat, with and without spices was compared. We show that meat snacks made from ostrich, beef, and chicken meat were characterized by high concentration of nutrients including proteins, minerals (heme iron especially in ostrich, than in beef), biologically active peptides (carnosine-in beef, anserine-in ostrich then in chicken meat). The, beneficial to human health, n-3 fatty acids levels differed significantly between species. Moreover, ostrich jerky contained four times less fat as compared to beef and half of that in chicken. In conclusion we can say that dried ostrich, beef, and chicken meat could be a good source of nutritional components.

The transect method: a novel approach to on-farm welfare assessment of commercial turkeys.

Currently, no animal-based protocol for on-farm welfare assessment of commercial turkeys is available. The birds' size and flighty nature make obtaining a representative sample using traditional methods difficult. The transect walks (TW) approach provides a potential alternative for on-farm assessments of turkey welfare. We compared the TW approach with a traditional method, and data collected as the birds were moved out of the house during the load out process (L). Ten commercial 19- to 20-week-old Hybrid turkey flocks were evaluated (1 flock/house/farm). Half of the flocks were housed on farms deemed as "faring well" by the company, the other half were on "suboptimal" farms. Each house was subdivided longitudinally into 4 transects. Two observers walked the transects in random order, recording the total number of birds per transect that were immobile; lame; aggressive towards a mate; interacting with humans; with visible head, vent, or back wounds; engaging in mounting behaviors; small; featherless; dirty; sick; terminal; or dead. Flocks were re-evaluated on the same day using the individual sampling method (S), where randomly selected birds were scored as they took 10 steps. Flocks were re-assessed within 48 h of the transect evaluation, as birds were funneled out of the house during load out. Using ANOVAs we determined the effects of observers, method, management, and their interactions on proportions of turkeys per house within each category. Outcome parameters were not affected by management (P>0.05 for all) or observer (P>0.05 for most), but an assessment method effect was detected (P<0.05). S differed from the 2 other methods (P<0.05) for most parameters except aggression towards a mate, back wounds, dirty, sick, and vent wounds. Differences were not detected between data collected using TW and during L, except for dead (P=0.0007) and immobile (P=0.007). Results suggest that the TW method is a promising tool for on-farm turkey welfare assessment as it produced results similar to those obtained at L when all birds could be scored individually.

Influence of stage of lactation and year season on composition of mares' colostrum and milk and method and time of storage on vitamin C content in mares' milk.

BACKGROUND: Mares' milk is becoming increasingly popular in Western Europe. This study was thus aimed at investigating the impact of stage of lactation and season on chemical composition, somatic cell count and some physicochemical parameters of mares' colostrum and milk, and at developing a method for the determination of vitamin C (ascorbic acid) in mares' milk and to determine its content in fresh and stored milk. RESULTS: The analysis conducted showed an effect of the stage of lactation on contents of selected chemical components and physicochemical parameters of mares' milk. In successive lactation periods levels of fat, cholesterol, energy value, citric acid and titratable acidity decreased, whereas levels of lactose and vitamin C, as well as the freezing point, increased. Analysis showed that milk produced in autumn (September, October, November) had a higher freezing point and lower concentrations of total solids, protein, fat, cholesterol, citric acid and energy value in comparison to milk produced in summer (June, July, August). Mares' milk was characterised by low somatic cell count throughout lactation. In terms of vitamin C stability the most advantageous method of milk storage was 6-month storage of lyophilised milk. CONCLUSION: In general, the results confirmed that mares' milk is a raw material with a unique chemical composition different from that produced by other farm animals.

Feeding frequency can affect the morphology of reproductive tract in broiler breeder hens.

This study examined the influence of feeding frequency on the morphology of the reproductive tract in broiler breeder hens, with a focus on the liver, ovary, and oviduct. A total of 364 hens and 52 roosters, starting at 31 wk of age and continuing until the end of their 38th week, were divided into 13 groups with varying feeding frequencies (1, 2, or 3 times daily). Significant changes in reproductive and metabolic parameters were observed, where feeding hens twice or thrice daily resulted in significantly reduced ovary weight and follicle counts (P < 0.001), while liver weight increased (P < 0.05). These findings suggesting complex interactions between feeding practices and reproductive efficiency.

The Aviary Transect-a practical welfare assessment tool to improve the management of cage-free laying hens.

The Aviary Transect (AT) is a method for assessing welfare in cage-free laying hen flocks, and comprises standardized walks along each aisle screening the flock for selected welfare indicators: feather loss (FL) on head, back, breast, and tail, wounds on head, back, tail, and feet, dirty plumage, enlarged crop, sickness, and birds found dead. The method is quick (20 min for a flock of 7,500 hens), has good interobserver agreement and shows positive correlations with individual bird sampling methods. However, it is less clear whether AT can be used to detect differences in flock health and welfare related to housing and management. The aim of this study was to evaluate how AT findings varied in relation to 23 selected housing, management, environmental, and production factors. The study was conducted on 33 commercial nonbeak-trimmed, white-feathered layer flocks of similar age (70-76 wk) kept in multitiered aviaries in Norway. The most prevalent findings across flocks were feather loss on the back (mean 0.97% of flock) and breast (0.94%) followed by feather loss on the head (0.45%) and tail (0.36%) with differences in feather pecking damage according to the hybrid used (P < 0.05). Better litter quality was associated with a lower prevalence of feather loss on the head and breast (P < 0.05), and addition of fresh litter during the production cycle resulted in fewer birds with feather loss on the head (P < 0.05) and tail (P < 0.001). Lower dust levels were linked to a lower prevalence of feather loss on the head, back, and breast (P < 0.05), and when access to the floor area underneath the aviary was provided at an earlier stage of production, fewer birds had wounds (P < 0.001), but more birds were observed with an enlarged crop (P < 0.05) and found dead (P < 0.05). In conclusion, findings from AT showed that results of the assessment varied according to housing conditions. These results support the validity of AT as a relevant welfare assessment tool for evaluating cage-free management practices.

Indicators of improved gestation housing of sows. Part I: Effects on behaviour, skin lesions, locomotion, and tear staining.

Commercial gestation housing systems for sows generally fail to cater fully for their needs in terms of comfort or the ability to perform highly motivated behaviours, which can lead to chronic stress and an impairment to welfare. We compared a typical gestation system (CONTROL) with an IMPROVED one as regards oral stereotypies, aggressive behaviour, skin lesions, locomotion, and tear staining. Sows were mixed into 12 stable groups (six groups per treatment, 20 sows per group), 29 days post-service in pens with free-access, full-length individual feeding/lying stalls. CONTROL pens had fully slatted concrete floors, with two blocks of wood and two chains suspended in the group area. IMPROVED pens were the same but with rubber mats and a length of manila rope in each feeding stall, and straw provided in three racks in the group area. Direct observations of oral stereotypical (30 instantaneous scans per sow per day) and aggressive (all-occurrence sampling, 3 h per sow per day) behaviours were conducted 72 h post-mixing, in mid and late gestation. Skin lesions were counted 24 h and three weeks post-mixing, and in late gestation. Sows' locomotion (locomotory ability) was scored using a visual analogue scale in mid and late gestation. Right and left eye tear staining was scored in late gestation. Indications of better welfare in IMPROVED sows included performance of fewer oral stereotypies in mid and late gestation, and lower tear stain scores. These sows performed more aggression in late gestation, which was associated with access to enrichment, but skin lesion counts were not affected. Thus, the changes made in the IMPROVED treatment benefitted aspects of sow welfare.

Linking key husbandry factors to the intrinsic quality of broiler meat.

Broiler farming is the fastest-growing animal production sector and broiler meat is the second most-consumed meat in the world. The intensification of broiler production often has a negative impact on the meat quality and carcass characteristics. Consumers, however, expect a quality product from animals reared extensively on farms providing good animal welfare, often intuitively associated with extensive farming practices. Therefore, this literature review investigates how the critical factors contributing to the degree of extensiveness of broiler production affect the quality of meat. We used the data from scientific articles published in the years 2012-2021 to analyze the effect of diet (n = 409), genetics (n = 86), enrichment (n = 25), and stocking density (n = 20) on meat quality and carcass characteristics. Minerals and microelements supplementation in the diet improved all the meat quality aspects: sensory, physical, and chemical in most studies. Minerals and enzymes in the diet had beneficial effects on carcass characteristics, unlike feed restriction and ingredient substitutions. The impact of outdoor access on meat quality and carcass characteristics was most frequently examined, in contrast to the use of perches or effects of litter quality. Overall, enrichment did not affect the meat's sensory or physical parameters, but outdoor access improved its lipid composition. Lower stocking density deteriorated intramuscular fat content, decreased tenderness and juiciness, yet lowered cooking and drip loss, and increased carcass and breast muscle yields. When it comes to genetics, in general, slow growing broiler strains have better meat quality parameters, especially regarding yellowness (b*), redness (a*), cooking and drip loss. Our review shows that the factors which contribute to extensiveness of broiler production systems and birds' welfare also affect meat quality and the carcass characteristics.

Indicators of improved gestation housing of sows. Part II: Effects on physiological measures, reproductive performance and health of the offspring.

Prenatal stress is the mechanism through which poor welfare of pregnant sows has detrimental effects on the health and resilience of their piglets. We compared two gestation housing systems (IMPROVED versus [conventional] CONTROL) in terms of sow stress and welfare indicators and sought to determine whether potential benefits to the sows would translate into improved offspring health. Sows were mixed into 12 stable groups (six groups per treatment, 20 sows per group) 29 days post-service in pens with free-access, full-length individual feeding/lying-stalls. CONTROL pens had fully slatted concrete floors, with two blocks of wood and two chains suspended in the group area. IMPROVED pens were the same but with rubber mats and manila rope in each stall, and straw provided in three racks in the group area. Saliva was collected from each sow on day 80 of pregnancy and analysed for haptoglobin. Hair cortisol was measured in late gestation. Sows' right and left eyes were scored for tear staining in mid lactation and at weaning. Numbers of piglets born alive, dead, mummified, and total born were recorded. Piglets were weighed and scored for vitality and intra-uterine growth restriction (IUGR) at birth. Presence of diarrhoea in farrowing pens was scored every second day throughout the suckling period. IMPROVED sows had lower haptoglobin levels and tear-stain scores during lactation. IMPROVED sows produced fewer mummified piglets, and these had significantly lower IUGR scores, and scored lower for diarrhoea than piglets of CONTROL sows. Hence, improving sow welfare during gestation improved the health and performance of their offspring.

Risk Factors for Chronic Stress in Sows Housed in Groups, and Associated Risks of Prenatal Stress in Their Offspring.

Chronic stress has a detrimental effect on sow welfare and productivity, as well as on the welfare and resilience of their piglets, mediated prenatally. Despite this, the specific risk factors for chronic stress in pregnant sows are understudied. Group-housed pregnant sows continuously face numerous challenges associated with aspects of the physical (group type and size, flooring, feeding system) and social (stocking density, mixing strategy) environment. There are many well-known potent stressors for pigs that likely contribute to chronic, physiological stress, including overcrowding, hot temperatures, feed restriction, inability to forage, uncomfortable floors, and poor handling. Some of these stressors also contribute to the development of production diseases such as lameness, which in turn are also likely causes of chronic stress because of the associated pain and difficulty accessing resources. The aim of this review is to discuss potential risk factors for chronic stress in pregnant sows such as space allowance, group size and type (stable/dynamic), feeding level, lameness, pen design, feed system, enrichment and rooting material, floor type, the quality of stockmanship, environmental conditions, and individual sow factors. The mechanisms of action of both chronic and prenatal stress, as well as the effects of the latter on offspring are also discussed. Gaps in existing research and recommendations for future work are outlined.

Developing a novel welfare assessment tool for loose-housed laying hens - the Aviary Transect method.

This study compared welfare assessment results in aviary flocks using 3 approaches: 1) A novel Aviary Transect method, 2) AssureWel, and 3) the Norwegian farm advisors' NorWel method. The Aviary Transect time requirement, interobserver reliability, and within- and across-house sensitivity to detect welfare indicators were also evaluated. The study was conducted on 6 randomly chosen commercial white-strain layer flocks of similar age and flock size, kept in multitiered aviaries. The Aviary Transect method comprised standardized walks along each aisle while screening the whole flock for 12 welfare indicators: feather loss (FL) on head, back, breast, and tail, wounds on head, back, tail, and feet, dirty birds, enlarged crop, sick birds, and dead birds. AssureWel involved scoring FL on head and back, and dirtiness of 50 random birds, and flock-level evaluation of beak trimming, antagonistic behavior, flightiness, birds needing further care, and mortality. NorWel involved scoring 8 welfare indicators on 50 random birds: FL on head, back, breast, and tail, dirtiness, and wounds on head, back, and tail. The AssureWel detected flock differences in both minor and major FL on the back (P < 0.01) as well as somewhat dirty birds (P < 0.01). The NorWel method detected flock differences in both minor and major FL on the head (P < 0.01), back (P < 0.001), breast (P < 0.001), and tail (P < 0.001) and somewhat (score 1) dirty birds (P < 0.05). The Aviary Transect method detected flock differences in FL on head, back, breast, and tail (all P < 0.001), dirty birds (P < 0.05) and enlarged crop (P < 0.001). More birds with FL on breast, and more dirty birds, were found in wall vs. central transects (P < 0.05). There was good interobserver agreement, except for dirty birds (P < 0.01), and positive correlations (P < 0.05) were identified between the Aviary Transect method and the other sampling methods for FL on head and back, and dirtiness. The three methods took similar time to complete (about 20 min/flock). In conclusion, all 3 methods detected significant differences in welfare indicator prevalence between flocks. The new Aviary Transect method provides egg producers with an efficient and sensitive whole-flock assessment of hen welfare status in multitiered aviaries.

Gut microbiota activity in chickens from two genetic lines and with outdoor-preferring, moderate-preferring, and indoor-preferring ranging profiles.

Despite the existing research into the gut microbiome of meat chickens, the associations between gut microbiome composition, its activity and chicken outdoor ranging frequency remain unexplored. The aim of this study was to determine the gut microbiota composition, activity and metabolic products in chickens of 2 different lines and 3 ranging profiles. Sixty non-beak trimmed birds, either Sasso or Green-legged Partridge were housed with access to outdoor ranges from wk. 5 to 10 of age. Outdoor ranges were video recorded to obtain frequencies of the birds' range use. The information about relative abundance of selected bacterial groups in the ceca including Lactobacillus spp., E. coli, Bifidobacterium spp., and Clostridium spp. was obtained with the PCR method. Gut microbiota activity was assessed based on the glycolytic activity of bacterial enzymes including, α-glucosidase, ß-glucosidase, α-galactosidase, ß-galactosidase, and ß-glucuronidase as well as based on the concentration of short-chain fatty acids (SCFA) in the caecal digesta. Statistical analysis was conducted by generalized linear mixed models, applying the breed and ranging profile as fixed effects and pen as a random factor. The lowest relative abundance of Bifidobacterium spp. was found in the cecal content of indoor-preferring Sasso birds (0.01 ± 0.001), as compared to all other birds in the experiment (ranging from 0.03 ± 0.01 to 0.11 ± 0.07; P = 0.0002). The lowest relative abundance of E. coli was identified for all outdoor-preferring birds and indoor- preferring Sasso birds (0.01 ± 0.001; P = 0.0087). Cecal activity of: α-glucosidase, ß-glucuronidase and ß-galactosidase was higher in Green-legged Partridges, as compared to Sasso (P = 0.013; P = 0.008; P = 0.004). Valeric acid concentrations were higher in moderate Green-legged Partridges than in Sasso of the same ranging profile (2.03 ± 0.16 vs. 1.5 ± 0.17; 0.016). The majority of the current results confirmed an effect of genotype and ranging profile on the various analyzed parameters. In outdoor-preferring birds, the consumption of pasture originating feed sources as a supplement to the indoor accessible cereal-based diet likely caused the positive effects on the birds' microbial profile.

Associations between animal-based measures at 11 wk and slaughter data at 20 wk in turkey toms (Meleagris gallopavo).

To monitor animal welfare on a commercial scale, systematic collection and evaluation of slaughterhouse data is the most feasible method. However, we need to know whether slaughterhouse data retrospectively and reliably reflect information about the birds' welfare on-farm. The aim of this study was therefore to investigate associations between animal-based welfare measures in flocks of turkey toms at 11 wk of age recorded with the transect walk and slaughter data at 20 wk of age. A total of 20 commercial flocks of turkey toms were visited, where an observer walked the transects in a random order and recorded the total number of birds per transect that were immobile, lame, with visible head, tail, or wing wounds, small, featherless, dirty, sick, terminal, or dead. Slaughterhouse data were provided for each flock. Univariate and multivariate linear regression models were used to investigate the associations between on-farm and slaughterhouse measures. Increased prevalence of immobile toms at week 11 resulted in more birds rejected at slaughter owing to leg issues (P = 0.02) and airsacculitis (P < 0.001). More lame birds on-farm were associated with an overall higher rejection rate at slaughter (P < 0.001). Flocks with more featherless birds had significantly more birds being rejected at slaughter owing to skin issues (P = 0.02). More dirty birds at week 11 resulted in more birds being rejected owing to airsacculitis at slaughter (P < 0.001). A higher mortality on-farm was associated with more birds rejected for being too small (P = 0.04). In conclusion, significant associations between animal-based measures of turkey toms as assessed by the transect walk method on-farm at 11 wk and slaughter data at 20 wk were identified.

Early Detection of Locomotion Disorders in Gilts Using a Novel Visual Analogue Scale; Associations with Chronic Stress and Reproduction.

Locomotion scoring is crucial for the early detection of lameness, which reduces sow welfare and performance. Our objective was to test the effectiveness of a visual analogue scale (VAS) to measure locomotory ability (OVERALL) compared to a categorical scoring system (CAT) and to investigate associations with hair cortisol and reproductive performance. Locomotion was scored in gilts (n = 51) at service, on day 57 and day 108 of pregnancy, and at weaning, using a VAS (150 mm line: 0 mm (perfect)-150 mm (severely lame)), and a CAT (1 (perfect)-5 (severely lame)). Hair cortisol concentration was measured on day 108 of pregnancy. Reproductive performance data (parity 1-4) were acquired from farm records. VAS detected deviations from optimal locomotion more effectively than the CAT (F3,145 = 2.70; p ≤ 0.05 versus F3,195 = 2.45; p = 0.065). Higher OVERALL scores at service (REG = 0.003 ± 0.0012; F1,48 = 4.25; p ≤ 0.05) and on day 57 (REG = 0.003 ± 0.0013; F1,48 = 6.95; p ≤ 0.05) were associated with higher hair cortisol concentrations on day 108. Positive associations were detected between OVERALL at service and the number of piglets born dead (REG = 0.01 ± 0.006; F1,36 = 4.24; p ≤ 0.05), and total born (REG = 0.1 ± 0.03; F1,120 = 4.88; p ≤ 0.05). The VAS better facilitates early detection of lameness, which could help to prevent detrimental effects, possibly mediated by chronic stress, on reproductive performance.

Cat owners' perception on having a pet cat during the COVID-19 pandemic.

Restrictions during the COVID-19 pandemic may affect the lives of pet animals. We aimed to assess the impact of the COVID-19 pandemic on the welfare of pet cats across countries, as well as the owners' experience in relation to their cat(s). An online survey containing 21 questions was distributed internationally. Questions were related to information about the cat and its behaviour and health, changes in the household due to the pandemic, and how the owner related to the cat. The survey was completed by 324 respondents from 25 different countries. The survey showed that keeping pet cats during the COVID-19 pandemic did not create problems for the owners, except some difficulties in acquiring veterinary care. The majority (67.3%) of respondents reported no changes in their cats' behaviour. When behavioural changes were reported, they were mostly of a positive nature. Owners who took more measures in relation to the cat to prevent the spread of the virus, e.g., reduced contact, were 1.8 times more likely to report changes in the behaviour of their cats but also 3.8 times more likely to report difficulties related to the care of the cat during the pandemic. Two-third of the respondents indicated a reduction of their own psychological tensions due to having a cat during the pandemic. We concluded that the welfare of pet cats, as reported by the owners, was not adversely affected by the pandemic and the human-cat interaction had positive effects on the owners during the lockdown.

The Activity of Chosen Antioxidant Enzymes in Ostrich Meat in Relation to the Type of Packaging and Storage Time in Refrigeration.

The aim of the study was to investigate the changes in the activity of antioxidant enzymes, i.e., superoxide dismutase (SOD), glutathione peroxidase (GPx) and glutathione reductase (GR), and 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activity in ostrich meat, as influenced by various packaging systems and storage time under refrigeration. Three packaging methods were used: vacuum packaging (VP) and modified atmosphere packaging (MAP) in two combinations of gases, MAP1 (40% O2/40% CO2/20% N2) and MAP2 (60% O2/30% CO2/10% N2). Meat samples were taken from the M. ilifibularis (IF) muscles of eight ostriches in each treatment group. The meat samples were stored in a refrigerator in 2 °C and analyzed at days 0, 4, 8, 12 and 16. The lowest level of SOD activity during storage was observed in ostrich muscles packed in vacuum, as compared to MAP1 and MAP2. In turn, the highest increase in GPx activity was recorded in VP, especially up to day 8 of storage, when this parameter reached maximum value (54.37). GR increased up to the eighth day of storage in MAP1 and VP. Between the 12th and 16th days of storage, stabilization of the GR activity level was observed only in VP, while under MAP1, it further decreased. DPPH remained relatively stable until the eighth day of storage and after this period, a decrease in this parameter was recorded, reaching the lowest value on day 12 for all types of packaging systems.