RESUMEN
Acute respiratory infection (ARI) is the most frequent pathology along human life, being the most common cause of morbidity and mortality in children under 5 years. The aim of this study was to determine the frequency of bocavirus (BoV) in infants under 5 years with symptoms of ARI from north Argentina (Chaco province). The study was performed on nasopharyngeal aspirates from 488 patients, in the period of January-December 2014. The samples were tested by real time PCR and 36 positive BoV cases (7.4%) were detected. The period with the highest detection rate was June-September with 28 cases (77.8%), of which 26 (72.2%) were infants between 6-18 moths of life. In half of BoV positive cases this virus was detected as single infection of the upper respiratory tract, and in the remaining 50%, as concomitant infection with other microorganisms. To our knowledge, this would be the first study on molecular epidemiology of BoV in northern Argentina. We emphasize the importance of investigating these new viruses capable of generating acute respiratory disease and also to disseminate awareness on their circulation within the community.
Asunto(s)
Bocavirus Humano/aislamiento & purificación , Infecciones por Parvoviridae/epidemiología , Infecciones por Parvoviridae/virología , Infecciones del Sistema Respiratorio/epidemiología , Infecciones del Sistema Respiratorio/virología , Enfermedad Aguda , Argentina/epidemiología , Preescolar , Femenino , Humanos , Lactante , Masculino , Nasofaringe/virología , Reacción en Cadena en Tiempo Real de la PolimerasaRESUMEN
Human papillomavirus (HPV) has the highest mortality rate due to cervical cancer in Northeastern Argentina. The aim of this work was to detect and characterize HPV in samples from the Province of Corrientes, Argentina. HPV detection and typing was performed using PCR-RFLP on samples with different cervical lesions (n=255). Seventeen viruses typified as HPV-58 were sequenced (E6 and E7 genes) and mutations were analyzed. HPV DNA was detected in 56.1% of the cervical lesions (143/255). Twenty-two different HPV types were detected. The type most frequently found among the total number of samples and HPV-positive samples was HPV-16 (14.5% and 25.9%, respectively), followed by HPV-58 (8.2%/14.7%, respectively), which is also considered a high-risk viral type. Increased severity of the cytological status was associated with greater rates of HPV detection and, especially, with the detection of greater rates of high-risk types. In addition, the evolutionary dynamics of the alpha-9 species group and HPV-58 was studied. All HPV-58 viruses reported in this work belonged to lineage A, sublineage A2. The phylodynamic analysis indicated that diversification of main groups within lineage A might have accompanied or preceded human migrations across the globe. Given that the most prevalent viruses found belonged to high-risk HPV types, some concerns might arise about the extent of cross protection of the vaccines against the types not included in their design.
Asunto(s)
Papillomaviridae/clasificación , Papillomaviridae/aislamiento & purificación , Infecciones por Papillomavirus/virología , Neoplasias del Cuello Uterino/virología , Adolescente , Adulto , Argentina , Femenino , Humanos , Persona de Mediana Edad , Papillomaviridae/genética , Filogenia , Prevalencia , Adulto JovenRESUMEN
The presence of Chlamydia trachomatis and human papillomavirus (HPV) was evaluated by PCR technique in 189 sexually active women, between 15 and 58 years old, with cytological abnormalities in their uterine cervical epithelium and belonging to a region of Argentina with high incidence of cervical cancer. Risk factors in relation to chlamydial infection were also analyzed. Total prevalence for C. trachomatis infection was 24.9%, but there was a significant difference between prevalence in low socio-economical level (32.9%) and high or medium socio-economical level (17.7%). Total prevalence for DNA of HPV was 52.9%, but women infected with C. trachomatis showed a higher risk for viral infection than non-infected ones (OR = 2.27 / CI 95% = 1.10-4.73), with statistical significant difference (p = 0.016).
Asunto(s)
Cuello del Útero/microbiología , Infecciones por Chlamydia/epidemiología , Chlamydia trachomatis , Papillomaviridae , Infecciones por Papillomavirus/epidemiología , Adolescente , Adulto , Argentina/epidemiología , Carcinoma/diagnóstico , Cuello del Útero/patología , Cuello del Útero/virología , Infecciones por Chlamydia/diagnóstico , Chlamydia trachomatis/aislamiento & purificación , Femenino , Genotipo , Humanos , Persona de Mediana Edad , Papillomaviridae/aislamiento & purificación , Infecciones por Papillomavirus/diagnóstico , Reacción en Cadena de la Polimerasa , Polimorfismo de Longitud del Fragmento de Restricción , Prevalencia , Factores de Riesgo , Neoplasias del Cuello Uterino/diagnóstico , Frotis VaginalRESUMEN
El género Klebsiella tiene importancia en hospitales y sanatorios por su tendencia a causar brotes. Una característica sobresaliente es la resistencia natural y/o adquirida a antimicrobianos. Según la red WHONET - Argentina, existe circulación de Klebsiella en nosocomios, particularmente en salas de terapia intensiva. En este trabajo se evaluó la presencia de Klebsiella spp. en 1233 pacientes de un sanatorio de la ciudad de Resistencia (Chaco), durante el período 2015 2017. La detección de la bacteria se realizó por cultivo y la sensibilidad se determinó por difusión en agar. Se detectó Klebsiella en 5.6% (69) de las muestras y multirresistencia en 84% de los aislamientos. Se concluye que existe circulación de Klebsiella en la institución, debe ser correctamente identificada y establecido su nivel de resistencia. Esto es fundamental para disminuir los fracasos terapéuticos
The Klebsiella genus is important in hospitals and sanatoriums because of its tendency to cause outbreaks. An outstanding characteristic is the natural and / or acquired resistance to antimicrobials. According to the WHONET-Argentina network, there is Klebsiella circulation in hospitals, particularly in intensive care rooms. In this work, we searched Klebsiella spp. in 1233 patients from a sanatorium in the city of Resistencia (Chaco), during the period 2015 - 2017. The detection of the bacteria was carried out by culture and the sensitivity was determined by agar diffusion. Klebsiella was detected in 5.6% (69) of the samples and multiresistance in 84% of the isolates. It is concluded that there is Klebsiella circulation in the institution, it must be correctly identified and its level of resistance established. This is essential to reduce therapeutic failures
Asunto(s)
Humanos , Bacterias/aislamiento & purificación , Infecciones Bacterianas/diagnóstico , Brotes de Enfermedades , Hospitales , Klebsiella , Casas de Salud , PacientesRESUMEN
El virus de la hepatitis B (HBV) presenta amplia distribución en todo el mundo, con más de 500 millones de individuos infectados. Este microorganismo pertenece a la familia Hepadnaviridae. En el presente trabajo se analizó la presencia del antígeno de superficie del virus (HBsAg) y perfil enzimático (GOT, GPT y FAL) en 292 muestras de suero o plasma de estudiantes universitarios de ambos sexos. De las muestras analizadas una sola resultó "Reactiva" para el HBsAg, lo que representa el 0.34 %, confirmado por técnica de reacción en cadena de la polimerasa.
Summary The Hepatitis B virus (HBV) is widely distributed throughout the world, with more than 500 million infected individuals. Is a virus belonging to the Hepnaviridae family. In the present work was analyzed the presence of HBsAg and enzymatic profile (GOT, GPT, FAL) in 292 serum samples or plasm of university students of both sexes. From analyzed samples so far, only one of them was "reactive" for HBsAg, which represents 0,34%. In addition, the determination of total anti-core antibodies was realised to the latter sample resulting "reactive
Asunto(s)
Hepadnaviridae , Virus de la Hepatitis B , Antígenos/análisisRESUMEN
La infección respiratoria aguda (IRA) es la patología más frecuente a lo largo de la vida de una persona y es la causa más común de morbi-motalidad en menores de 5 años. El objetivo de este trabajo fue determinar la frecuencia de bocavirus (BoV) en pacientes menores de 5 años con diagnóstico presuntivo de IRA en una ciudad capital del norte argentino (Resistencia, Chaco). Se analizaron aspirados nasofaríngeos correspondientes a 488 niños durante el año 2014. Los mismos fueron testeados por PCR en tiempo real hallándose BoV en 36 casos (7.4%), de los cuales 26 (72.2%) fueron infantes de 6-18 meses de vida. La mayor concentración de positivos se registró en el período junio-septiembre con un total de 28 pacientes (77.8%). Los casos positivos para BoV se observaron como infección única en el 50% de los mismos y el resto como infecciones concomitantes con otros microorganismos. No conocemos que haya otro estudio de epidemiología molecular de BoV en el norte argentino y destacamos la importancia de investigar los nuevos virus capaces de generar infección respiratoria aguda, y difundir el conocimiento de su circulación en la comunidad.
Acute respiratory infection (ARI) is the most frequent pathology along human life, being the most common cause of morbidity and mortality in children under 5 years. The aim of this study was to determine the frequency of bocavirus (BoV) in infants under 5 years with symptoms of ARI from north Argentina (Chaco province). The study was performed on nasopharyngeal aspirates from 488 patients, in the period of January-December 2014. The samples were tested by real time PCR and 36 positive BoV cases (7.4%) were detected. The period with the highest detection rate was June-September with 28 cases (77.8%), of which 26 (72.2%) were infants between 6-18 moths of life. In half of BoV positive cases this virus was detected as single infection of the upper respiratory tract, and in the remaining 50%, as concomitant infection with other microorganisms. To our knowledge, this would be the first study on molecular epidemiology of BoV in northern Argentina. We emphasize the importance of investigating these new viruses capable of generating acute respiratory disease and also to disseminate awareness on their circulation within the community.
Asunto(s)
Humanos , Masculino , Femenino , Lactante , Preescolar , Infecciones del Sistema Respiratorio/epidemiología , Infecciones del Sistema Respiratorio/virología , Infecciones por Parvoviridae/epidemiología , Infecciones por Parvoviridae/virología , Bocavirus Humano/aislamiento & purificación , Argentina/epidemiología , Nasofaringe/virología , Enfermedad Aguda , Reacción en Cadena en Tiempo Real de la PolimerasaRESUMEN
Acute respiratory infection (ARI) is the most frequent pathology along human life, being the most common cause of morbidity and mortality in children under 5 years old. The aim of this study was to determine the circulation of HBoV in infants under 5 years old with symptoms of ARI from north Argentina (Chaco province). Were analyzed 882 nasopharyngeal aspirates obtained during the years 2014 and 2015. The samples were tested by real time PCR and the overal frequency of HBoV was 7.6%. The period with the major detection report was June-September with 77.6% of all positive cases. It is interesting that 98.5% of all cases were in infants aged 0 to 2 years of life. HBoV positive cases were given as single infection in 46.3% of patients and the remaining 53.7% as concomitant infections with other viruses. The evaluation of two full years calendar of molecular epidemiological study in northern Argentina of HBoV, reflects more accurately the current situation and could lead to re-formulate strategies for diagnosis, treatment and prognosis of this disease. We intend to highlight the importance of investigate this new virus capable of produce an acute respiratory disease by providing information about it to the community.
A infecção respiratória aguda (IRA) é a mais frequente ao longo da vida de uma patologia individual e é a causa mais comum de mortalidade/morbidade em crianças com menos de 5 anos. O objetivo deste estudo foi determinar o movimento do Bocavírus Humano (HBoV) em crianças menores de 5 anos com um diagnóstico presuntivo de IRA numa capital estadual do norte da Argentina (Resistencia, Chaco). Foram analizados 882 aspirados nasofaríngeos, obtidos durante os anos 2014 e 2015. As pessoas foram testeadas por PCR em tempo real, onde se encontrou uma frequência de HBoV do 7,6% (67/882), dando a maior concentração de casos positivos no período de junho a setembro (77,6%). Curiosamente, 98,5% dos casos foram em crianças de 0 a 2 anos de vida. Os casos positívos de HBoV foram dados como única infecção em 46,3% dos pacientes e os restantes 53,7% como infecções concomitantes com outros vírus. A avaliação de dois anos, calendário completo de estudo de epidemiologia molecular HBoV no norte da Argentina, reflete com mais precisão a realidade, e pode reformular as estratégias para o diagnóstico, tratamento e prognóstico da patología. Temos a intenção de destacar a importância de se investigar o novo vírus capaz de gerar doença respiratória aguda, fornecendo informações dele para a comunidade
La infección respiratoria aguda (IRA) es la patología más frecuente a lo largo de la vida de una persona y es la causa más común de morbi-motalidad en niños menores de 5 años. El objetivo de este trabajo fue determinar la circulación del Bocavirus Humano (HBoV) en niños menores de 5 años con diagnóstico presuntivo de IRA en una ciudad capital del norte argentino (Resistencia, Chaco). Ruiz Díaz, Natalia; y Col. Rev. Fac. Med. UNNE XXXVI: 3, 5-12, 2016 Se analizaron 882 aspirados nasofaríngeos obtenidos durante los años 2014 y 2015. Los mismos fueron testeados por PCR en tiempo real encontrándose una frecuencia de HBoV de 7.6% (67/882), dándose la mayor concentración de casos positivos en el período junio-septiembre (77,6%) y es interesante destacar que el 98,5% del total de casos se registraron en infantes entre 0 a 2 año de vida. Los casos positivos para HBoV se dieron como infección única en el 46.3% de los pacientes y el 53.7% restante como infecciones concomitantes con otros virus. La evaluación de dos años -calendarios completos- de estudio epidemiológico molecular de HBoV en el norte argentino, refleja de manera más fiel la realidad ya que permite reformular las estrategias en cuanto al diagnóstico, el tratamiento y el pronóstico de esta patología. Pretendemos destacar la importancia de investigar los nuevos virus capaces de generar patología respiratoria aguda brindando información sobre el mismo a la comunidad
Asunto(s)
Humanos , Preescolar , Enfermedades Respiratorias/patología , Infecciones del Sistema Respiratorio/diagnóstico , Preescolar , Infecciones por Parvoviridae/diagnóstico , Bocavirus Humano/patogenicidadRESUMEN
Human papillomavirus (HPV) has the highest mortality rate due to cervical cancer in Northeastern Argentina. The aim of this work was to detect and characterize HPV in samples from the Province of Corrientes, Argentina. HPV detection and typing was performed using PCR-RFLP on samples with different cervical lesions (n = 255). Seventeen viruses typified as HPV-58 were sequenced (E6 and E7 genes) and mutations were analyzed. HPV DNA was detected in 56.1 % of the cervical lesions (143/255). Twenty-two different HPV types were detected. The type most frequently found among the total number of samples and HPV-positive samples was HPV-16 (14.5 % and 25.9 %, respectively), followed by HPV-58 (8.2 %/14.7 %, respectively), which is also considered a high-risk viral type. Increased severity of the cytological status was associated with greater rates of HPV detection and, especially, with the detection of greater rates of high-risk types. In addition, the evolutionary dynamics of the alpha-9 species group and HPV-58 was studied. All HPV-58 viruses reported in this work belonged to lineage A, sublineage A2. The phylodynamic analysis indicated that diversification of main groups within lineage A might have accompanied or preceded human migrations across the globe. Given that the most prevalent viruses found belonged to high-risk HPV types, some concerns might arise about the extent of cross protection of the vaccines against the types not included in their design.
El virus del papiloma humano (Human papillomavirus [HPV]) tiene la mayor tasa de mortalidad por cáncer de cuello uterino en el noreste de Argentina. El objetivo de este trabajo fue detectar y caracterizar el HPV en muestras de la provincia de Corrientes, Argentina. La detección y la tipificación se realizó mediante PCR-RFLP en muestras con diferentes lesiones cervicales (n=255). Se secuenciaron 17 virus tipificados como HPV-58 (genes E6 y E7) y se analizaron sus mutaciones. Además, se estudió la dinámica evolutiva de los virus del grupo alfa-9 y, en particular, del HPV-58. Se detectó ADN viral en el 56,1% de las lesiones cervicales (143/255) y se detectaron 22 tipos del HPV. El tipo encontrado con mayor frecuencia entre el total de muestras y entre las HPV-positivas fue el HPV-16 (14,5%/25,9%, respectivamente), seguido por el HPV-58 (8,2%/14,7%, respectivamente), también considerado como de alto riesgo. El aumento de la gravedad de las lesiones se asoció a mayores tasas de detección del HPV y, en especial, con mayores tasas de detección de tipos de alto riesgo. Todos los HPV-58 encontrados pertenecieron al linaje A, sublinaje A2. El análisis filodinámico indicó que la diversificación de los grupos principales dentro del linaje A podría haber acompañado o precedido las migraciones humanas en todo el mundo. Dado que los virus más prevalentes pertenecieron a los tipos del HPV de alto riesgo, podrían surgir interrogantes sobre el alcance de la protección cruzada de las vacunas contra los tipos no incluidos en su diseño