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Ternary quantum processors offer significant potential computational advantages over conventional qubit technologies, leveraging the encoding and processing of quantum information in qutrits (three-level systems). To evaluate and compare the performance of such emerging quantum hardware it is essential to have robust benchmarking methods suitable for a higher-dimensional Hilbert space. We demonstrate extensions of industry standard randomized benchmarking (RB) protocols, developed and used extensively for qubits, suitable for ternary quantum logic. Using a superconducting five-qutrit processor, we find an average single-qutrit process infidelity of 3.8×10^{-3}. Through interleaved RB, we characterize a few relevant gates, and employ simultaneous RB to fully characterize crosstalk errors. Finally, we apply cycle benchmarking to a two-qutrit CSUM gate and obtain a two-qutrit process fidelity of 0.85. Our results present and demonstrate RB-based tools to characterize the performance of a qutrit processor, and a general approach to diagnose control errors in future qudit hardware.
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Studies in insect gustation have a long history in general physiology, particularly with work on fly labellar and tarsal sensilla and in the general field of insect-plant interactions, where work on immature Lepidoptera and chrysomelid beetles has been prominent. Much more emphasis has been placed on the physiological characteristics of the sensory cells than on the central cellular mechanisms of taste processing. This is due to the fairly direct access for physiological experimentation presented by many taste sensilla and to the obvious importance of tastants in insect feeding and oviposition behaviour. In some of the insect models used for gustatory studies, advances have been made in understanding the basic morphology of the central neuropils involved in the first stages of taste processing. There is much less known about the physiology of interneurons involved. In this review, we concentrate on four insect models (Manduca sexta, Drosophila melanogaster, Neobellieria bullata (and other large flies), and Apis mellifera) to summarize morphological knowledge of peripheral and central aspects of insect gustation. Our views of current interpretations of available data are discussed and some important areas for future research are highlighted.
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Insectos , Animales , Abejas/anatomía & histología , Abejas/fisiología , Sistema Nervioso Central/anatomía & histología , Sistema Nervioso Central/fisiología , Drosophila melanogaster/anatomía & histología , Drosophila melanogaster/fisiología , Manduca/anatomía & histología , Manduca/fisiología , Sistema Nervioso Periférico/anatomía & histología , Sistema Nervioso Periférico/fisiología , Gusto/fisiologíaRESUMEN
BACKGROUND: Localization of parathyroid glands is critical in the treatment of recurrent or persistent hyperparathyroidism. Technetium sestamibi imaging may improve localization; however, the mechanism of visualization of parathyroid tissue remains unclear. On the basis of the chemical structure of sestamibi it has been suggested that p-glycoprotein is involved in the transport of sestamibi across cell membranes. This study was designed to examine sestamibi uptake and retention and p-glycoprotein expression in normal and abnormal parathyroid tissue. METHODS: Thirty-two consecutive patients underwent 2-methoxy-isobutyl-isonitrile imaging immediately before parathyroid exploration. Tissue was obtained from normal and abnormal parathyroids and from the thyroid gland. Touch preparations gave rapid confirmation of tissue origin. Specimens were trimmed and weighed, and gamma-emission was counted. Percentage injected dose per gram of tissue was calculated. Immunohistochemistry was obtained with a battery of monoclonal antibodies to identify p-glycoprotein in parathyroid tissue submitted for permanent histologic examination. Slides were graded by a pathologist familiar with immunohistochemistry. RESULTS: Abnormal parathyroid tissue had a higher mean retention of injected dose per gram than did normal thyroid and parathyroid tissue. Immunohistochemistry revealed that abnormal parathyroid tissue expresses less p-glycoprotein. CONCLUSIONS: These results suggest that size is not the single determinant of parathyroid visualization and that p-glycoprotein expression may be involved in the mechanism of parathyroid imaging.
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Miembro 1 de la Subfamilia B de Casetes de Unión a ATP/fisiología , Glándulas Paratiroides/diagnóstico por imagen , Glándulas Paratiroides/metabolismo , Radiofármacos , Tecnecio Tc 99m Sestamibi , Miembro 1 de la Subfamilia B de Casetes de Unión a ATP/metabolismo , Adenoma/metabolismo , Adulto , Anciano , Femenino , Humanos , Hiperparatiroidismo/diagnóstico por imagen , Hiperparatiroidismo/metabolismo , Inmunohistoquímica , Masculino , Persona de Mediana Edad , Neoplasias de las Paratiroides/metabolismo , Cintigrafía , Valores de Referencia , Glándula Tiroides/citología , Glándula Tiroides/metabolismoRESUMEN
Patients with hyperparathyroidism who have not had previous neck surgery do not require preoperative localization because of the high success rate of cervical exploration (95%) and the limited sensitivity and specificity of all imaging modalities currently in use. Successful parathyroid exploration requires knowledge of the normal and frequently encountered variations in parathyroid anatomy (Fig. 4). Experience permits recognition of often subtle multiple gland disease. In skilled surgical hands, results are excellent with minimal morbidity. When recurrent or persistent disease or previously operated patients are encountered, confirmation of the diagnosis and attempts at localization should precede operation. Technetium sestamibi SPECT imaging and ultrasonography with FNA of suspicious glands are complementary tests that are readily available, inexpensive, and well tolerated by patients. If these tests are unsuccessful, MRI, CT, and invasive procedures should be pursued until the gland is localized.
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Hiperparatiroidismo/patología , Glándulas Paratiroides/patología , Biopsia con Aguja , Humanos , Hiperparatiroidismo/diagnóstico , Hiperparatiroidismo/diagnóstico por imagen , Hiperparatiroidismo/cirugía , Cuidados Intraoperatorios , Imagen por Resonancia Magnética , Glándulas Paratiroides/cirugía , Reoperación , Tecnecio Tc 99m Sestamibi , Tomografía Computarizada de Emisión de Fotón Único , Tomografía Computarizada por Rayos X , UltrasonografíaRESUMEN
Sensillae on the antennae of the Colorado potato beetle, Leptinotarsa decemlineata are described using scanning (SEM) and transmission (TEM) electron microscopy and compared with SEM observations of antennal sensilla in L. haldemani and L. texana. In all the three species, 13 distinct sensillar types were identified with a higher density of sensilla in the more polyphagous species, L. decemlineata than in the moderately host specific L. haldemani and the highly host specific L. texana. Cuticular specializations and the predominance of olfactory sensilla are discussed in relation to host specificity in the three species.
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Escarabajos/ultraestructura , Células Receptoras Sensoriales/ultraestructura , Olfato , Animales , Microscopía Electrónica , Microscopía Electrónica de Rastreo , Neuronas Receptoras Olfatorias/ultraestructuraRESUMEN
Three approaches were used to investigate effects of host plant epicuticular waxes on oviposition site selection by Plutella xylostella (L.). In the first approach, oviposition on canola (Brassica napus L.) that had epicuticular wax reduced by application of a carbamate herbicide (S-ethyl dipropylthiocarbamate) was compared with oviposition on untreated control plants. A second approach compared oviposition on sibling strains of B. napus with different wax blooms (glossy and waxy), and a third approach compared oviposition by P. xylostella on parafilm that had been applied to glossy and waxy B. napus strains for transfer of leaf components. Significantly more eggs were deposited on herbicide-treated plants (with reduced epicuticular wax) than on untreated controls. Similarly, more eggs were deposited on glossy than on waxy sibling strains of B. napus. In parafilm assays significantly more eggs were deposited on treated than on untreated parafilm. Several mechanisms could explain the differences in attractiveness of surfaces with varying wax content as oviposition sites for P. xylostella, including visual, chemical, and tactile differences between substrates. These mechanisms are discussed.
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Brassica/fisiología , Mariposas Nocturnas/fisiología , Animales , Femenino , Oviposición/fisiologíaAsunto(s)
Electrocardiografía/tendencias , Servicios Médicos de Urgencia/normas , Infarto del Miocardio/terapia , Terapia Trombolítica/normas , Factores de Tiempo , Electrocardiografía/instrumentación , Humanos , Infarto del Miocardio/diagnóstico , Infarto del Miocardio/epidemiología , Terapia Trombolítica/estadística & datos numéricos , Estados Unidos/epidemiologíaRESUMEN
The galeal chemosensory system of the adult Colorado potato beetle was used as a model to study the effects of alkaloids on insect gustation. Nine alkaloids, representing a wide range of structural types, were used. Their ability to stimulate chemosensory cells when presented in isolation and their ability to interfere with normal chemosensory processes were emphasized. None of the alkaloids stimulated chemosensory cells in a dose-dependent manner, although a few stimulated low-level activity from some cells. There was no evidence for a general "deterrent receptor" in these beetles. Some of the alkaloids had a marked inhibitory effect on normal chemosensory responses. Tomatine, solanine, papaverine, and sparteine significantly inhibited responses to amino acids (represented by GABA) while quinine and papaverine inhibited responses to sucrose. An attempt was made to correlate neurophysiological action of some alkaloids with their effects on feeding behavior. It was clear from this correlation that even a dramatic inhibition of sensory input by an alkaloid does not necessarily lead to measurable effects on behavior. The results are discussed in the context of current theories on the mode of action of alkaloids and other secondary plant compounds which may be involved in host recognition by phytophagous insects.
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Electrophysiological recordings have been made from cells in the eight large, labellar sensilla of g. morsitans. One of these cells in each sensillum was shown to respond to ATP over a concentration range of 10(-6)-10(-3) M. It was also sensitive to several other adenosine phophates, but much less sensitive to CTP, GTP and ITP. The activity of the receptor was depressed below pH 7, and sometimes considerably increased above pH 9. These aspects the receptor's physiology support the results of behavioural studies. It is concluded that the eight receptors mediate the flies' behavioural response to ATP.
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Adenosina Trifosfato , Receptores de Droga/fisiología , Moscas Tse-Tse/fisiología , Potenciales de Acción , Sitios de Unión , Células Quimiorreceptoras/fisiología , Conducta Alimentaria/fisiología , Concentración de Iones de Hidrógeno , Relación Estructura-Actividad , Moscas Tse-Tse/anatomía & histologíaRESUMEN
Steroidal glycoalkaloids, found in species of the Solanaceae, elicit bursting activity in galeal and tarsal chemosensilla of adult Colorado potato beetles. The effect has an average latency of 6-12 sec, depending on the sensillum/alkaloid combination. A 20-sec alkaloid treatment is often suffficient to render galeal sensilla unresponsive to gamma-aminobutyric acid, normally an effective stimulant. The alkaloids have similar effects on galeal sensilla of larval Colorado potato beetles and on labellar chemosensilla of the blowfly. It is concluded that these compounds act independently of any specialized chemoreceptor in the Colorado potato beetle, and that association of the Colorado potato beetle with solanaceous plants has not led to evolution of a specific receptor forSolanum glycoalkaloids.
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We used a detailed analysis of feeding behavior to investigate the role of solanaceous alkaloids as sensory-based feeding deterrents for the Colorado potato beetle,Leptinotarsa decemlineata (Say). Experiments were conducted on three geographic, host-adapted populations of beetles to determine whether evolutionary changes in host use have been accompanied by behavioral adaptations to alkaloids. Solanine and tomatine, steroidal glycoalkaloids found in two regional host plants, did not reduce leaf consumption or significantly alter behavior patterns of newly emerged beetles, including those from populations that normally will not feed on plants containing the compounds. Atropine, a tropane alkaloid found in several taxonomically related nonhost species, caused a significant increase in sampling behavior (indicating direct action on the sensory system) and reduced acceptance of treated potato leaves. We propose that variable acceptance of host plants among regional populations ofL. decemlineata has evolved independently of adaptations to alkaloids at the sensory level. To establish that secondary compounds such as atropine influence host choice in nature, field observations are needed to confirm that beetles routinely encounter, and sample, nonhost species.
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Larvae and adults of the Colorado potato beetle,Leptinotarsa decemlineata (Say), are shown to have galeal gustatory cells that are highly sensitive to distillate of potato leaf extracts, (E)-2-hexen-1-ol, (E)-2-hexenal, and other saturated and unsaturated six-carbon alcohols. In larvae and adults, the sensory response patterns elicited by leaf homogenate, leaf distillate and a mixture of these two extracts differ in subtle ways. Beetle larvae feed most readily on Millipore disks treated with leaf homogenate and the mixture, but they did not feed on disks treated with leaf distillate. The differences in behavioral response and sensory input are used to derive a potential gustatory code that may stimulate different levels of feeding. This code may be disrupted by compounds present in nonhost leaves, thus leading to reduced feeding. Possible interactions of sapid leaf volatiles, amino acids, sugars, and potentially deterrent plant compounds are discussed.
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Intracellular recordings were made from interneurons in the subesophageal ganglion (SEG) of Sarcophaga bullata while stimulating the labellar lobes with solutions of sucrose, NaCl and with distilled water. Neurons that responded to sucrose did not respond to NaCl and vice versa, while sucrose-sensitive neurons often responded weakly to water. Several of the recorded neurons were filled with Lucifer Yellow, and their morphology was reconstructed. Most showed extensive arborizations within the SEG, suggesting that they were local interneurons involved in the early stages of gustatory processing. Some of the filled neurons had extensive projections to the brain, in addition to arborizations in the SEG. This is the first published record of gustatory interneurons in the higher flies.
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Células Quimiorreceptoras/fisiología , Dípteros/fisiología , Ganglios/fisiología , Interneuronas/fisiología , Mecanorreceptores/fisiología , Gusto/fisiología , Animales , Encéfalo/anatomía & histología , Encéfalo/citología , Electrodos , Conducta Alimentaria/fisiología , Colorantes Fluorescentes , Ganglios/citología , Iontoforesis , IsoquinolinasRESUMEN
A mutant Escherichia coli (Ppcc-) which was unable to grow on glucose as a sole carbon source was isolated. This mutant had very low levels of phosphoenolpyruvate carboxylase activity (approximately 5% of the wild type). Goat immunoglobulin G prepared against wild-type phosphoenolypyruvate carboxylase cross-reacted with the Ppcc- enzyme. The amount of enzyme protein in the mutant cells was similar to that found in wild-type cells, but it had greatly diminished specific activity. The catalytically less active mutant enzyme retained the ability to interact with fructose 1,6-bisphosphate, but did not exhibit stabilization of the tetrameric form by aspartate. The pI of the mutant protein was lower (4.9) than that of the wild-type protein (5.1). After electrophoresis and immunoblotting of the partially purified protein, several immunostaining bands were seen in addition to the main enzyme band. A novel method for showing that these bands represented proteolytic fragments of phosphoenolpyruvate carboxylase was developed.
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Carboxiliasas/metabolismo , Escherichia coli/enzimología , Fosfoenolpiruvato Carboxilasa/metabolismo , Anticuerpos Antibacterianos/inmunología , Especificidad de Anticuerpos , Centrifugación por Gradiente de Densidad , Electroforesis en Gel de Poliacrilamida , Escherichia coli/genética , Fructosadifosfatos/metabolismo , Genes Bacterianos , Punto Isoeléctrico , Cinética , Mutación , Fosfoenolpiruvato/metabolismo , Fosfoenolpiruvato Carboxilasa/genética , Fosfoenolpiruvato Carboxilasa/inmunología , Fosfoenolpiruvato Carboxilasa/aislamiento & purificación , Pruebas de PrecipitinaRESUMEN
Because the benefits from thrombolytic therapy in acute myocardial infarction (AMI) are time dependent, multiple strategies have been devised to speed therapy. This study sought to determine whether hospital-based nurse and paramedic advanced life support (ALS) providers could be trained to independently evaluate (sight read) a prehospital 12-lead electrocardiogram (ECG) for the presence of AMI as part of a protocol designed to speed in-hospital administration of thrombolytic agents. Providers were required to determine on the basis of a protocol (1) whether or not AMI was present, and (2) whether or not thrombolytic therapy was indicated. Providers then radioed their impression to the emergency department (ED) and initiated a protocol to prepare identified candidates for thrombolysis. The final decision to initiate thrombolytic therapy was made by the ED physician after patient arrival at the hospital. One hundred fifty-five patients with chest pain were studied. Twenty-one (13.5%) were ultimately proven in-hospital to have AMI. Providers were able to recognize AMI in 17 of 21. Four of 21 did not meet ECG criteria for AMI on the field ECG, but were categorized as having a high index of suspicion for AMI by providers. There were no false-positive diagnoses. Fourteen patients (9%) received thrombolytic therapy. In-hospital times to administration of thrombolytic therapy decreased to an average of 22 +/- 13.8 minutes in the studied group compared with a historical control group average of 51 +/- 50 minutes. It is concluded that hospital-based paramedics and nurses can successfully be taught to evaluate (ie, sight read) a prehospital ECG for the presence of AMI with accuracy.(ABSTRACT TRUNCATED AT 250 WORDS)