Isotemporal Associations of Device-Measured Sedentary Time and Physical Activity with Cardiac-Autonomic Regulation in Previously Pregnant Women.

BACKGROUND: High sedentary time (ST) and low physical activity may increase cardiovascular risk, potentially though cardiac-autonomic dysregulation. This study investigated associations of statistically exchanging device-measured ST and physical activity with measures of cardiac-autonomic regulation in previously pregnant women. METHOD: This cross-sectional, secondary analysis included 286 women (age = 32.6 ± 5.7 years; 68% white) measured 7-15 years after delivery. ST and light (LPA), moderate (MPA), vigorous (VPA), and moderate-to-vigorous (MVPA) intensity physical activity were measured by ActiGraph GT3X. ST was further partitioned into long (≥ 30 min) and short (< 30 min) bouts. MVPA was also partitioned into long (≥ 10 min) and short (< 10 min) bouts. Cardiac-autonomic regulation was assessed by heart rate variability (HRV) (resting heart rate, natural log transformed standard deviation of normal R-R intervals [lnSDNN], natural log-transformed root mean square of successive differences [lnRMSSD]) from a 5-min seated ECG. Progressive isotemporal substitution models adjusted for confounders. Sensitivity analyses removed women with related underlying medical conditions and who did not meet respiration rate criteria. RESULTS: Initial analyses found no significant associations with HRV when exchanging 30 min of ST and physical activity (p > 0.05). Yet, replacing long- and short-bout ST with 30 min of long-bout MVPA yielded significantly higher (healthier) lnRMSSD (B = 0.063 ± 0.030 and B = 0.056 ± 0.027, respectively; both p < 0.05). Sensitivity analyses strengthened these associations and yielded further associations of higher lnSDNN and lnRMSSD when replacing 30 min of short-bout MVPA with equivalent amounts of long-bout MVPA (B = 0.074 ± 0.037 and B = 0.091 ± 0.046, respectively). CONCLUSION: Replacing ST with long-bout MVPA is a potential strategy to improve cardiac-autonomic function in previously pregnant women.

Cardiovascular responses to physical activity during work and leisure.

OBJECTIVES: Recent evidence suggests that occupational physical activity (OPA) is associated with adverse cardiovascular health, whereas leisure time physical activity is protective. This study explored explanatory physiological mechanisms. METHODS: Nineteen males (68% white, age=46.6±7.9 years, body mass index=27.9±5.1 kg/m2) with high self-reported OPA wore activity (ActiGraph and activPAL) and heart rate (HR) monitors for 7 days and an ambulatory blood pressure (BP) monitor on one workday and one non-workday. Mixed effects models compared cardiovascular variables (24-hour, nocturnal, waking and non-work time HR and BP) and nocturnal HR variability (HRV) on workdays versus non-workdays. Additional models examined associations of daily activity (steps, light physical activity (LPA) and moderate-to-vigorous physical activity (MVPA)) with cardiovascular variables. Workday by daily activity interactions were examined. RESULTS: 24-hour and waking HR and diastolic BP as well as non-work diastolic BP were significantly higher on workdays versus non-workdays (p<0.05 for all). However, no difference in systolic BP or nocturnal HR or BP was observed between work and non-workdays (p>0.05 for all). Low-frequency and high-frequency power indices of nocturnal HRV were lower on workdays (p<0.05 for both). Daily steps and LPA were positively associated with 24-hour and waking HR on work and non-workdays. Significant interactions suggested MVPA increases HR and lowers nocturnal HRV during workdays, with the opposite effect on non-workdays. CONCLUSIONS: Cardiovascular load was higher on workdays versus non-workdays with no compensatory hypotensive response following workdays. Daily MVPA may differentially affect ambulatory cardiovascular load and nocturnal HRV on workdays versus non-workdays, supporting the physical activity health paradox hypothesis.

Tethered Swimming Test: Reliability and the Association With Swimming Performance and Land-Based Anaerobic Performance.

ABSTRACT: Nagle Zera, J, Nagle, EF, Nagai, T, Lovalekar, M, Abt, JP, and Lephart, SM. Tethered swimming test: reliability and the association with swimming performance and land-based anaerobic performance. J Strength Cond Res 35(1): 212-220, 2021-The purpose of this study was 3-fold: (a) to examine the test-retest reliability of a 30-second maximal tethered freestyle swimming test (TST), (b) to assess the validity of the TST by examining the association with sprint swimming performance, and (c) to examine the associations between a swim-specific and land-based measure of anaerobic performance. A total of 29 male and female swimmers were recruited to participate in the study. Each subject completed a Wingate Anaerobic cycling test (WAnT), 2 or 4 TST, and a 22.9 m (25 yd), 45.7 m (50 yd), and 91.4 m (100 yd) maximal freestyle performance swims (PS). Mean and peak force (Fmean and Fpeak) were recorded for both the WAnT and TST, and average swimming velocity and time were recorded for the PS. In addition, physiological and perceptual measures were recorded immediately postexercise for all tests. The results of the present investigation showed strong intersession and intrasession reliability (R = 0.821-0.975; p < 0.001) for force parameters of the TST. Moderate correlations were found between Fmean and PS time and velocity of all distances, with slightly weaker correlations between Fpeak and the 22.9 m (time and velocity) and 45.7 m (velocity) PS. Finally, moderate correlations were found for Fmean and Fpeak of the TST and WAnT. This study demonstrated that the TST is a reliable measure, with moderate association with swimming performance, producing similar physiological responses compared with free swimming. Therefore, future research should focus on investigating the potential benefits of using the TST as a regular assessment tool as a part of a competitive swimming training program to track adaptations and inform training decisions.

Reliability and Validity of a Pool-Based Maximal Oxygen Uptake Test to Examine High-Intensity Short-Duration Freestyle Swimming Performance.

Nagle, EF, Nagai, T, Beethe, AZ, Lovalekar, MT, Zera, JN, Connaboy, C, Abt, JP, Beals, K, Nindl, BC, Robertson, RJ, and Lephart, SM. Reliability and validity of a pool-based maximal oxygen uptake test to examine high-intensity short-duration freestyle swimming performance. J Strength Cond Res 33(5): 1208-1215, 2019-A modality-specific swimming protocol to assess maximal oxygen uptake (V[Combining Dot Above]O2maxsw) is essential to accurately prescribe and monitor swimming conditioning programs. Consequently, there is a need for a reliable and valid graded intensity swimming pool test to accurately assess V[Combining Dot Above]O2maxsw using indirect calorimetry. The purpose of this study was to assess (a) reliability of an intensity self-regulated swimming pool test of V[Combining Dot Above]O2maxsw and (b) validity of a V[Combining Dot Above]O2maxsw test using performance swim (PS) time as the criterion. Twenty-nine men (n = 15) and women (n = 14) (age, 23 ± 6.4 years; body mass index, 23.5 ± 3.0 kg·m) performed 2 swimming pool V[Combining Dot Above]O2maxsw trials (V[Combining Dot Above]O2maxsw A and V[Combining Dot Above]O2maxsw B), and 2 PS tests (45.7 m [31.20 ± 4.5 seconds] and 182 m [159.2 ± 25.5 seconds]). For test-retest reliability (trials A vs. B), strong correlations (p < 0.05) were found for V[Combining Dot Above]O2maxsw (ml·kg·min) (r = 0.899), O2 pulse (ml O2·beat) (r = 0.833), and maximum expired ventilatory volume (L·min) (r = 0.785). For performance validity, moderately strong correlations (p < 0.05) were found between V[Combining Dot Above]O2maxsw A and 45.7-m (r = -0.543) and 182-m (r = -0.486) swim times. The self-regulated graded intensity swimming pool protocol examined presently is a reliable and valid test of V[Combining Dot Above]O2maxsw. Studies should consider the suitability of a V[Combining Dot Above]O2maxsw test for military personnel, clinical populations, and injured athletes.

Identification and Biochemical Characterization of a Novel Protein Phosphatase 2C-Like Ser/Thr Phosphatase in Escherichia coli.

In bacteria, signaling phosphorylation is thought to occur primarily on His and Asp residues. However, phosphoproteomic surveys over the past decade in phylogenetically diverse bacteria have identified numerous proteins that are phosphorylated on Ser and/or Thr residues. Consistently, genes encoding Ser/Thr kinases are present in many bacterial genomes, such as that of Escherichia coli, which encodes at least three Ser/Thr kinases. Since Ser/Thr phosphorylation is a stable modification, a dedicated phosphatase is necessary to allow reversible regulation. Ser/Thr phosphatases belonging to several conserved families are found in bacteria. One family of particular interest are Ser/Thr phosphatases, which have extensive sequence and structural homology to eukaryotic Ser/Thr protein phosphatase 2C (PP2C) phosphatases. These proteins, called eukaryote-like Ser/Thr phosphatases (eSTPs), have been identified in a number of bacteria but not in E. coli Here, we describe a previously unknown eSTP encoded by an E. coli open reading frame (ORF), yegK, and characterize its biochemical properties, including its kinetics, substrate specificity, and sensitivity to known phosphatase inhibitors. We investigate differences in the activity of this protein in closely related E. coli strains. Finally, we demonstrate that this eSTP acts to dephosphorylate a novel Ser/Thr kinase that is encoded in the same operon.IMPORTANCE Regulatory protein phosphorylation is a conserved mechanism of signaling in all biological systems. Recent phosphoproteomic analyses of phylogenetically diverse bacteria, including the model Gram-negative bacterium Escherichia coli, demonstrate that many proteins are phosphorylated on serine or threonine residues. In contrast to phosphorylation on histidine or aspartate residues, phosphorylation of serine and threonine residues is stable and requires the action of a partner Ser/Thr phosphatase to remove the modification. Although a number of Ser/Thr kinases have been reported in E. coli, no partner Ser/Thr phosphatases have been identified. Here, we biochemically characterize a novel Ser/Thr phosphatase that acts to dephosphorylate a Ser/Thr kinase that is encoded in the same operon.

Reliability and Accuracy of a Standardized Shallow Water Running Test to Determine Cardiorespiratory Fitness.

A standardized fitness assessment is critical for the development of an individualized exercise prescription. Although the benefits of aquatic exercise have been well established, there remains the need for a standardized nonswimming protocol to accurately assess cardiorespiratory fitness (CRF) in shallow water. The present investigation was designed to assess (a) the reliability of a standardized shallow water run (SWR) test of CRF and (b) the accuracy of a standardized SWR compared with a land-based treadmill (LTM) test. Twenty-three healthy women (20 ± 3 years), with body mass index (23.5 ± 3 kg·m), performed 2 shallow water peak oxygen consumption (V[Combining Dot Above]O2peak) running tests (SWRa and SWRb), and 1 V[Combining Dot Above]O2max LTM. Intraclass correlation coefficients indicated moderately strong reliability for V[Combining Dot Above]O2peak (ml·kg·min) (r = 0.73, p < 0.01), HRpeak (b·min) (r = 0.82; p < 0.01), and O2pulse (V[Combining Dot Above]O2 [ml·kg·min]·HR [b·min]) (r = 0.77, p < 0.01). Using paired t-tests and Pearson's correlations, SWR V[Combining Dot Above]O2peak and HRpeak were significantly lower than during LTM (p ≤ 0.05) and showed moderate correlations of 0.60 and 0.58 (p < 0.001) to LTM. O2pulse was similar (p > 0.05) for the SWR and LTM tests with a moderate correlation of 0.63. A standardized SWR test as a measure of CRF is a reliable, and to some degree, valid alternative to conventional protocols and may be used by strength and conditioning professionals to measure program outcomes and monitor training progress. Furthermore, this protocol provides a water-based option for CRF assessment among healthy women and offers insight toward the development of an effective protocol that can accommodate individuals with limited mobility, or those seeking less musculoskeletal impact from traditional land-based types of training.

Energy Drinks Improve Five-Kilometer Running Performance in Recreational Endurance Runners.

Prins, PJ, Goss, FL, Nagle, EF, Beals, K, Robertson, RJ, Lovalekar, MT, and Welton, GL. Energy drinks improve five-kilometer running performance in recreational endurance runners. J Strength Cond Res 30(11): 2979-2990, 2016-The purpose of this study was to evaluate exercise performance time and related physiological and perceptual responses of recreational endurance runners after they had ingested a commercially available energy drink (Red Bull, Red Bull GmbH, Fuschl am See, Austria) containing caffeine, glucose, and taurine. Recreational endurance runners (n = 18; 13 men and 5 women; age: 20.39 ± 3.27 years; weight: 71.25 ± 17.17 kg; height: 178.00 ± 7.57 cm; V[Combining Dot Above]O2max: 55.94 ± 7.66 ml·kg·min) participated in a double-blind, crossover, repeated-measures study where they were randomized to supplement with 500 ml of the commercially available energy drink Red Bull and a noncaffeinated, sugar-free placebo (PLA) 60 minutes before completing a 5-km time trial on a treadmill, separated by 7 days. Heart rate, rating of perceived exertion (RPE) (RPE-Overall; RPE-Chest; RPE-Legs), and affect were recorded at rest, 1 hour before ingestion, at 5-minute intervals during the 5-km time trial, and immediately after exercise. Session RPE and session affect were obtained 5 minutes after completion of the 5-km time trial. The distance covered at each 5-minute interval during the 5-km time trial was recorded. Performance improved with the energy drink compared with placebo (Red Bull: 1,413.2 ± 169.7 vs. PLA: 1,443.6 ± 179.2 seconds; p = 0.016), but there were no differences in RPE, affect, session RPE, session affect, or the distance covered at 5-minute splits between the two 5-km time trials (p > 0.05). These results demonstrate that consuming a commercially available energy drink before exercise can improve 5-km performance. These results may have application for altering pre-exercise nutritional strategies in recreational runners.

Prediction of VO2 peak using OMNI Ratings of Perceived Exertion from a submaximal cycle exercise test.

The primary aim of this study was to develop statistical models to predict peak oxygen consumption (VO2 peak) using OMNI Ratings of Perceived Exertion measured during submaximal cycle ergometry. Male (M = 20.9 yr., SE = 0.4) and female (M = 21.6 yr., SE = 0.5) participants (N = 81) completed a load-incremented maximal cycle ergometer exercise test. Simultaneous multiple linear regression was used to develop separate VO2 peak statistical models using submaximal ratings of perceived exertion for the overall body, legs, and chest/breathing as predictor variables. VO2 peak (L·min(-1)) predicted for men and women from ratings of perceived exertion for the overall body (3.02 ± 0.06; 2.03 ± 0.04), legs (3.02 ± 0.06; 2.04 ± 0.04), and chest/breathing (3.02 ± 0.05; 2.03 ± 0.03) were similar to measured VO2 peak (3.02 ± 0.10; 2.03 ± 0.06, ps > .05). Statistical models based on submaximal OMNI Ratings of Perceived Exertion provide an easily administered and accurate method to predict VO2 peak.

Session perceived exertion and affective responses to self-selected and imposed cycle exercise of the same intensity in young men.

Session perceived exertion (S-RPE) and session affective responses (S-AR) are post-exercise estimates of the global responses experienced during exercise. To compare S-RPE and S-AR to acute RPE (A-RPE) and acute AR (A-AR) during self-selected (SS) and imposed (IMP) exercise of the same workload. Thirty-two males (22.3 ± 2.2 years) performed two, 20-min cycle exercise trials. In the SS trial, subjects adjusted SS workload every 5 min. In the IMP trial, workload was automatically adjusted to the SS workload. Experimental (EXP, n = 16) subjects were unaware that workload was the same between the trials. Control (CON, n = 16) subjects were aware that both trials were of the same workload. A-RPE and A-AR were measured every 5 min using the OMNI Scale and Feeling Scale, respectively. Fifteen minutes following a cool-down, subjects rated S-RPE and S-AR. Session and exercise values were compared between trials and groups using ANOVA. No between-group differences were observed. There were no differences between the SS and IMP trials for S-RPE, A-RPE, S-AR and A-AR. For SS and IMP trials, S-RPE was greater than A-RPE (4.6 ± 1.5 vs. 3.9 ± 1.4; 4.3 ± 1.6 vs. 3.7 ± 1.4, respectively, p < 0.05). S-AR was greater than A-AR for the SS trial (1.9 ± 1.3 vs. 2.3 ± 1.5, p < 0.05), but not the IMP trial (1.9 ± 1.5 vs. 2.2 ± 1.4). A mismatch exists between the session and acute exercise values for RPE and AR during the SS cycle exercise in young males.

Just noticeable difference in perception of physical exertion during cycle exercise in young adult men and women.

The purpose of this investigation was to describe the just noticeable difference (JND) in perceived exertion during cycle exercise. Males (n = 20) and females (n = 26) (21.4 ± 3.1 year) performed load-incremented cycle exercise to peak intensity. At the end of each minute, subjects rated their overall-body perceived exertion using the OMNI (0-10) rating of perceived exertion (RPE) scale. Individual regression derived the power output (PO) corresponding to RPE 5. This PO served as the standard stimulus (SS). On a separate occasion, four 5-min cycling bouts were performed with 5 min rest between bouts. During bouts 1 and 3 subjects cycled at the SS. During bouts 2 and 4 subjects adjusted the resistance to achieve a level of exertion just noticeably above/below the SS. The difference in final 30-s oxygen consumption (VO2) and PO between each JND bout and the previous SS were the above (JND-A) and below (JND-B) perceived exertion JNDs. JND-A and JND-B were compared between genders and between subjects exhibiting lower versus higher ventilatory threshold (VT) and VO(2PEAK) within genders for VO2 (l · min(-1), %VO(2PEAK)) and PO (W, %SS). JND-B was significantly (P < 0.05) greater than JND-A for VO2 and PO, when expressed in absolute (l · min(-1), W) and relative units (%VO(2PEAK), %SS). Males exhibited greater JND values than females in absolute, but not relative, units. Subjects with lower and higher VT and VO(2)PEAK exhibited similar JND values. The JND can serve as an effective tool to measure perceptual acuity and to determine individual ability to self-regulate prescribed exercise intensities.

Energy expenditure, cardiorespiratory, and perceptual responses to shallow-water aquatic exercise in young adult women.

INTRODUCTION: Aquatic exercise (AE) is a popular form of physical activity, yet few studies have assessed the individual's energy expenditure (EE) associated with a continuous bout of AE. Studies using indirect calorimetry to measure EE have reported limitations associated with test methodology and the ability to control individual's exercise intensity or tempo. PURPOSE: To evaluate EE and cardiorespiratory (CR) responses during a 40-minute shallow-water AE session in young adult women. METHODS: Twenty-one healthy women (aged 21.7 ± 3.4 years) completed an orientation practice session and a 40-minute shallow-water AE session using a traditional exercise class format and the SWEAT video-based instructional cuing program. The high-intensity interval format included the following segments: 1) warm-up (3 minutes); 2) CR segment ( 22 minutes); 3) muscular endurance segment (ME; 10 minutes); and 4) cool-down (5 minutes). Subject oxygen consumption (VO2; mL/kg/min), heart rate (HR) and OMNI overall ratings of perceived exertion (RPE-O) were assessed each minute. Average kcal/min1, metabolic equivalents (METs; 1 MET = 3.5 mL/kg/min), and total kcals per segment and for the overall session were calculated. RESULTS: The total subject EE throughout the 40-minute trial (including warm-up and cool-down segments) was 264 kcals, with an overall average of 6.3 kcals/min (5.6 METs).The average kcals/min expended throughout CR segments 2 through 6 was 8.05 (7.1 METs), with the Hoverjog segment producing the greatest average kcals/min at 8.3 (7.3 METs). The CR portion (22 min) contributed 65% of the total EE (171 kcals) of the 40-minute AE trial. For the overall AE trial, the highest and average subject VO2 achieved were 33.3 and 19.7 mL/kg/min, respectively. The average highest subject heart rate achieved was 177 beats per minute (bpm), equivalent to 90% of the participant's age-predicted HRmax. CONCLUSION: Energy expenditure during a 40-minute AE session met national recommendations for a daily moderate-to-vigorous bout of physical activity offering a viable alternative to land-based exercise. Because AE serves as a partial-weight bearing modality, future studies are needed to clarify the EE of shallow-water AE in apparently healthy and clinical populations.

Self-efficacy and enjoyment of middle school children performing the progressive aerobic cardiovascular endurance run (PACER).

Self-efficacy and enjoyment were examined among 34 middle school children (M age = 12.5 yr.) performing the Progressive Aerobic Cardiovascular Endurance Run (PACER). Exercise self-efficacy (running) and physical activity enjoyment were measured after viewing a video illustrating the PACER, and subsequently following a PACER test. Significantly greater pre- than post-exercise self-efficacy was reported; enjoyment scores did not differ. Ratings of self-efficacy were higher before exercise than after, but enjoyment scores were not significantly different. A significant correlation was found between post-exercise self-efficacy and enjoyment, but not between pre-exercise self-efficacy and enjoyment. Although positive correlations were found between PACER laps and pre-/post-exercise self-efficacy, correlations with ratings of enjoyment were not significant. Exercise self-efficacy was associated with children's beliefs about the task-specific PACER aerobic exercise; however, exercise enjoyment was stable. Children's self-efficacy and enjoyment beliefs should be considered when developing interventional strategies to promote aerobic exercise participation.

Reliability and Validity of a Flume-Based Maximal Oxygen Uptake Swimming Test.

A mode-specific swimming protocol to assess maximal aerobic uptake (VO2maxsw) is vital to accurately evaluate swimming performance. A need exists for reliable and valid swimming protocols that assess VO2maxsw in a flume environment. The purpose was to assess: (a) reliability and (b) "performance" validity of a VO2maxsw flume protocol using the 457-m freestyle pool performance swim (PS) test as the criterion. Nineteen males (n = 9) and females (n = 10) (age, 28.5 ± 8.3 years.; height, 174.7 ± 8.2 cm; mass, 72.9 ± 12.5 kg; %body fat, 21.4 ± 5.9) performed two flume VO2maxsw tests (VO2maxswA and VO2maxswB) and one PS test [457 m (469.4 ± 94.7 s)]. For test-retest reliability (Trials A vs. B), moderately strong relationships were established for VO2maxsw (mL·kg-1·min-1)(r= 0.628, p = 0.002), O2pulse (mL O2·beat-1)(r = 0.502, p = 0.014), VEmax (L·min-1) (r = 0.671, p = 0.001), final test time (sec) (0.608, p = 0.004), and immediate post-test blood lactate (IPE (BLa)) (0.716, p = 0.001). For performance validity, moderately strong relationships (p < 0.05) were found between VO2maxswA (r =-0.648, p = 0.005), O2pulse (r= -0.623, p = 0.008), VEmax (r = -0.509 p = 0.037), and 457-m swim times. The swimming flume protocol examined is a reliable and valid assessment of VO2maxsw., and offers an alternative for military, open water, or those seeking complementary forms of training to improve swimming performance.

Loss of receptor activity-modifying protein 3 exacerbates cardiac hypertrophy and transition to heart failure in a sex-dependent manner.

Sex differences exist in the hypertrophic response, cardiac remodeling, and transition to heart failure of hypertensive patients, and while some of these differences are likely influenced by estrogen, the genetic pathways downstream of estrogen that impact on cardioprotection have yet to be fully elucidated. We have previously shown that the cardioprotective effects of adrenomedullin (AM), an emerging clinical biomarker for cardiovascular disease severity, vary with sex in mouse models. AM signaling during cardiovascular stress is strongly modulated by receptor activity-modifying protein 3 (RAMP3) via its interaction with the G protein-coupled receptor calcitonin receptor-like receptor (CLR). Like AM, RAMP3 expression is potently regulated by estrogen, and so we sought to determine the consequences of genetic Ramp3 loss on cardiac adaptation to chronic hypertension, with a particular focus on characterizing potential sex differences. We generated and bred RAMP3(-/-) mice to RenTgMK mice that consistently display severe angiotensin II-mediated CV disease and compared CV disease progression in RenTgMK to that of RenTgMK:RAMP3(-/-) offspring. As expected, RAMP3 gene expression was higher in cardiovascular tissues of RenTgMK mice and more strongly up-regulated in female RenTgMK mice relative to wildtype controls. RAMP3 loss did not affect the development of hypertension or the presence and severity of perivascular and interstitial fibrosis in the left ventricle (LV). However, echocardiography revealed that while RenTgMK mice developed concentric cardiac hypertrophy with sustained systolic function, male RenTgMK:RAMP3(-/-) mice showed evidence of LV chamber dilatation and depressed systolic function, suggestive of cardiac decompensation. Consistent with these measures of heart failure, male RenTgMK:RAMP3(-/-) mice had increased cardiac apoptosis and elevated activation of Akt. These phenotypes were not present in female RenTgMK:RAMP3(-/-) mice. Collectively, these data demonstrate a sex-dependant, cardioprotective role of RAMP3 in the setting of chronic hypertension.

Physical Activity in the Workplace: Does Just Working Meet Activity Recommendations?

Background: The physical activity (PA) health paradox hypothesizes that occupational physical activity (OPA) and leisure time PA have differential cardiovascular health effects due to increased cardiovascular load without adequate recovery; however, research describing worker PA lacks high-quality objective OPA measurement. This study aimed to objectively describe PA profiles of men reporting high OPA and make comparisons to aerobic PA and OPA recommendations. Methods: Male food service, material moving, health care, or maintenance workers wore activity (ActiGraph® and activPAL®) and heart rate monitors for 7 days. Participants recorded work, non-work, and sleep times in a diary. PA was operationalized as time spent in sedentary behavior, upright time, light, moderate, vigorous, and moderate-to-vigorous PA during work and non-work hours. PA profiles were described and compared with Centers for Disease Control and Prevention aerobic PA guidelines (≥21.4 minute/day) and OPA recommendations (<30 minute/hour upright and intensity of <30% heart rate reserve). Findings: Nineteen male workers (68% White, age = 46.6±7.9 years) were more active on workdays than non-workdays (sedentary: 492.3 vs. 629.7 minute/day; upright: 462.4 vs. 325.2 minute/day; moderate-to-vigorous PA: 72.4 vs. 41.5 minute/day, respectively; all p < .05). Most participants (17/19) achieved aerobic PA guidelines across all days with more achieving on workdays (19/19) than non-workdays (13/19). OPA often exceeded recommended limits with participants accumulating 39.6±12.2 minutes/work hour upright and 30.3±25.9% of working time >30% heart rate reserve. Conclusions/Application to Practice: Male workers reporting high OPA typically met aerobic PA guidelines but exceeded recommended OPA limits. The long-term health implications of such activity profiles should be investigated.

Gender Differences and the Influence of Body Composition on Land and Pool-Based Assessments of Anaerobic Power and Capacity.

Consistent differences between males and females have been shown in land-based measurements of anaerobic power and capacity. However, these differences have not been investigated for a tethered 30-s maximal swimming test (TST). The purpose of this study is to explore gender differences in land and pool-based assessments of anaerobic power (Fpeak) and capacity (Fmean), as well as the influence of body composition. Thirteen males and fifteen females completed land (Wingate (WAnT)) and pool-based (TST) measures of anaerobic power and capacity previously described in the literature. Additionally, the subjects completed assessments of body composition via air displacement plethysmography. The males produced higher force than the females for Fpeak (p < 0.001) and Fmean (p = 0.008) during the TST. However, linear regression analysis determined that lean mass significantly predicted Fpeak (p = 0.002) and Fmean (p < 0.001) during the TST, while gender was no longer significant (p = 0.694 and p = 0.136, respectively). In conclusion, increases in anaerobic power and capacity (Fpeak and Fmean) may be a function of increased lean mass in males and females, warranting future research on the impact of resistance training programs on force production and swimming performance.

Association of Physical Activity and Sedentary Time with Cardio-Autonomic Regulation in Women.

Background: Low moderate-to-vigorous-intensity physical activity (MVPA) and high sedentary time (ST) may contribute to cardiovascular disease (CVD) risk in women, perhaps via cardiac autonomic dysregulation. We examined associations of total, leisure, and occupational MVPA and ST with cardiac autonomic regulation in women. Methods: Data were from 522 women (age = 37.7 ± 5.7 years; 59%white) who participated in the follow-up study of the Pregnancy Outcomes and Community Health Study (between 2011 and 2014). MVPA and ST (hours/day) were self-reported using the Modifiable Activity Questionnaire. Cardiac autonomic regulation was assessed by calculating heart rate variability (HRV) indices (resting heart rate, natural logarithm standard deviation of normal R-R intervals; lnSDNN [total variability], natural logarithm root mean square of the successive differences; lnRMSSD [cardiac parasympathetic activity]) with Kubios software from a 5-minute, seated electrocardiogram. Progressive generalized linear models evaluated associations of total, leisure, and occupational MVPA and ST with HRV indices while adjusting for confounders (demographics, health-related factors), and then potential mediators (clinical variables). A final model evaluated the relationship between ST and HRV stratified by MVPA level. Results: Adjusting for confounders, total and leisure MVPA were associated with favorable lnSDNN (B = 0.027 [p = 0.014] and B = 0.074 [p = 0.009], respectively) and lnRMSSD (B = 0.036 [p = 0.015] and B = 0.075 [p = 0.043], respectively). Adjustment for mediators tended to strengthen the observed significant associations. No associations were found between occupational MVPA or any ST measure with HRV indices (p > 0.05). Neither MVPA nor ST were associated with heart rate. When stratified by MVPA level, leisure ST was associated with unfavorable lnRMSSD (B = -0.041, [p = 0.042]) only among women who did not meet leisure MVPA recommendations. Conclusion: Cardiac autonomic dysregulation may be a mechanism through which low leisure MVPA and, among low-active women, high leisure ST contribute to CVD risk among women.

Response normalized omni rating of perceived exertion at the ventilatory breakpoint in division I football players.

This investigation identified an OMNI Scale rating of perceived exertion associated with the ventilatory breakpoint in Division I football players. This easily applied prescriptive reference may be used to self-regulate training intensity. The OMNI Scale format may facilitate estimation of exertional perceptions. 34 participants completed a maximal treadmill test. OMNI ratings of perceived exertion were estimated during the last 15 sec. of each workload. The rating corresponding to the ventilatory breakpoint was determined for each participant using linear regression analysis. The ventilatory breakpoint corresponded to 68.8% of VO2max (SD = 7.5% of VO2 max) and a heart rate of 158.9 beats min(-1) (SD = 11.9 beats min(-1)). The rating of perceived exertion associated with the ventilatory breakpoint was 5.1 (SD = 1.2). This perceptual reference point, not previously identified in Division I football players, could be used in field settings to self-select exercise intensity around the ventilatory threshold.

Use of ratings of perceived exertion to anticipate treadmill test termination in patients taking beta-blockers.

This investigation identified a perceptually-based "warning zone" that can be used to anticipate termination of treadmill tests administered to individuals taking beta-blockers. The use of ratings of perceived exertion to anticipate test termination may be valuable given the attenuation of heart rate associated with the use of this class of medication. Sixteen men with coronary artery disease participated in this investigation. Ratings of perceived exertion (Borg 6-20 Scale) were estimated during the last 15 sec. of each minute of a progressively incremented treadmill test. Tests were terminated when participants indicated they were too fatigued to continue. The time-to-test termination was determined from the point that participants estimated a rating of 10, 11, 12, 13, 14, 15, 16, and 17 in order to determine the rating that was less than or equal to the time associated with a single exercise stage. A rating of 14 was associated with impending termination, which can be used as a "warning zone." The time to termination corresponding to a rating of 14 was 153.1 sec. (SD = 27.0). Participants exercised for 153.1 sec. (SE = 27.0) after estimating a rating of 14. Once a rating of 14 is reached, volitional test termination will occur before the next exercise stage is completed.