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1.
Pol J Vet Sci ; 19(3): 567-572, 2016 Sep 01.
Artículo en Inglés | MEDLINE | ID: mdl-27760033

RESUMEN

In this study, dogs were separated into two groups and treated with immunosuppressant (Cyclosporin A: CsA). The first group was the canine atopic dermatitis (CAD) group, which is similar to extrinsic atopic dermatitis (AD) in humans (treated with a CsA dose of 2.5-5.5 mg/kg, n=8), and the second group was the canine atopic-like dermatitis (ALD) group, which is similar to intrinsic AD in humans (treated with a CsA dose of 2.5-6.5 mg/kg, n=14). The canine atopic dermatitis extent and severity index (CADESI)-4 was evaluated before treatment (PRE) and after treatment (POST) to assess the effectiveness of CsA for the two groups. In the CAD group, CADESI-4 showed no change (PRE:79±29, POST:77±28) and out of the eight dogs, no dogs showed complete remission, three dogs showed partial remission, and five dogs showed no effect. Whereas in the ALD group, CADESI-4 showed a significant reduction (PRE: 61±42, POST: 32±25, p<0.01) and out of the 14 dogs, 11 dogs showed complete remission, two dogs showed partial remission, and one dog showed no effect. The results indicate that the immunosuppressant showed effectiveness for the dogs diagnosed with ALD. One dog had to be treated for a year and eight months, which was the longest period in the study, this dog presented with hyperplasia of the lymphoidgland and mammary tumor.


Asunto(s)
Ciclosporina/uso terapéutico , Dermatitis Atópica/veterinaria , Enfermedades de los Perros/tratamiento farmacológico , Inmunosupresores/uso terapéutico , Animales , Dermatitis Atópica/tratamiento farmacológico , Perros
2.
Pol J Vet Sci ; 17(2): 371-3, 2014.
Artículo en Inglés | MEDLINE | ID: mdl-24988868

RESUMEN

This study investigated effects of a fluoxetine (selective serotonin reuptake inhibitors; SSRI, 1 mg/kg) on pruritus in canine atopic dermatitis (CAD). After 4-weeks of base-line observation, 8 dogs with CAD entered a 2-months randomized, double-blind, placebo-controlled, crossover trial comparing fluoxetine with placebo. Clinical efficacy was evaluated using a Canine Atopic Dermatitis Extent and Severity Index (CADESI-03) and Pruritus Visual Analog Scale (PVAS). Six dogs completed the study [two out of eight dogs (both of them were Shiba Inu) dropped out from the study due to a depression]. CADESI-03 and PVAS between fluoxetine and placebo showed no significant difference statistically (P > 0.05 and P > 0.05 respectively). Fluoxetine showed no efficacy on pruritus in CAD. Further researches are needed for the treatment on pruritus of CAD.


Asunto(s)
Dermatitis Atópica/veterinaria , Fluoxetina/uso terapéutico , Animales , Estudios Cruzados , Dermatitis Atópica/tratamiento farmacológico , Perros , Método Doble Ciego , Femenino , Masculino
3.
Genes Immun ; 12(7): 523-30, 2011 Oct.
Artículo en Inglés | MEDLINE | ID: mdl-21654846

RESUMEN

Although several major histocompatibility complex (MHC)-wide single-nucleotide polymorphism (SNP) studies have been performed in populations of European descent, none have been performed in Asian populations. The objective of this study was to identify human leukocyte antigen (HLA) loci associated with multiple sclerosis (MS) in a Japanese population genotyped for 3534 MHC region SNPs. Using a logistic regression model, two SNPs (MHC Class III SNP rs422951 in the NOTCH4 gene and MHC Class II SNP rs3997849, susceptible alleles A and G, respectively) were independently associated with MS susceptibility (204 patients; 280 controls), two (MHC Class II SNP rs660895 and MHC Class I SNP rs2269704 in the NRM gene, susceptible alleles G and G, respectively) with aquaporin-4- (AQP4-) MS susceptibility (149 patients; 280 controls) and a single SNP (MHC Class II SNP rs1694112, susceptible allele G) was significant when contrasting AQP4+ against AQP4- patients. Haplotype analysis revealed a large susceptible association, likely DRB1*04 or a locus included in the DRB1*04 haplotype, with AQP4- MS, which excluded DRB1*15:01. This study is the largest study of the HLA's contribution to MS in Japanese individuals.


Asunto(s)
Antígenos HLA/genética , Esclerosis Múltiple/genética , Polimorfismo de Nucleótido Simple , Alelos , Pueblo Asiatico/genética , Femenino , Estudios de Asociación Genética , Cadenas HLA-DRB1/genética , Haplotipos , Humanos , Japón , Masculino , Fenotipo
4.
Science ; 271(5248): 505-8, 1996 Jan 26.
Artículo en Inglés | MEDLINE | ID: mdl-8560265

RESUMEN

An RNA virus, designated hepatitis G virus (HGV), was identified from the plasma of a patient with chronic hepatitis. Extension from an immunoreactive complementary DNA clone yielded the entire genome (9392 nucleotides) encoding a polyprotein of 2873 amino acids. The virus is closely related to GB virus C (GBV-C) and distantly related to hepatitis C virus, GBV-A, and GBV-B. HGV was associated with acute and chronic hepatitis. Persistent viremia was detected for up to 9 years in patients with hepatitis. The virus is transfusion-transmissible. It has a global distribution and is present within the volunteer blood donor population in the United States.


Asunto(s)
Virus de Hepatitis/genética , Hepatitis Viral Humana/virología , Virus ARN/genética , Reacción a la Transfusión , Enfermedad Aguda , Secuencia de Aminoácidos , Secuencia de Bases , Donantes de Sangre , Patógenos Transmitidos por la Sangre , Enfermedad Crónica , Clonación Molecular , Secuencia de Consenso , Transmisión de Enfermedad Infecciosa , Flaviviridae/genética , Genoma Viral , Virus de Hepatitis/química , Virus de Hepatitis/aislamiento & purificación , Hepatitis Viral Humana/epidemiología , Hepatitis Viral Humana/transmisión , Humanos , Datos de Secuencia Molecular , Reacción en Cadena de la Polimerasa , Virus ARN/química , Virus ARN/aislamiento & purificación , ARN Viral/sangre , ARN Viral/genética , Alineación de Secuencia , Estados Unidos/epidemiología , Proteínas Virales/química , Proteínas Virales/genética , Viremia/epidemiología , Viremia/virología
5.
Mol Cell Biol ; 12(10): 4384-90, 1992 Oct.
Artículo en Inglés | MEDLINE | ID: mdl-1328854

RESUMEN

In order to analyze the transcriptional regulation of the muscle-specific subunit of the human phosphoglycerate mutase (PGAM-M) gene, chimeric genes composed of the upstream region of the PGAM-M gene and the bacterial chloramphenicol acetyltransferase (CAT) gene were constructed and transfected into C2C12 skeletal myocytes, primary cultured cardiac muscle cells, and C3H10T1/2 fibroblasts. The expression of chimeric reporter genes was restricted in skeletal and cardiac muscle cells. In C2C12 myotubes and primary cultured cardiac muscle cells, the segment between nucleotides -165 and +41 relative to the transcription initiation site was sufficient to confer maximal CAT activity. This region contains two E boxes and one MEF-2 motif. Deletion and substitution mutation analysis showed that a single MEF-2 motif but not the E boxes had a substantial effect on skeletal and cardiac muscle-specific enhancer activity and that the cardiac muscle-specific negative regulatory region was located between nucleotides -505 and -165. When the PGAM-M gene constructs were cotransfected with MyoD into C3H10T1/2, the profile of CAT activity was similar to that observed in C2C12 myotubes. Gel mobility shift analysis revealed that when the nuclear extracts from skeletal and cardiac muscle cells were used, the PGAM-M MEF-2 site generated the specific band that was inhibited by unlabeled PGAM-M MEF-2 and muscle creatine kinase MEF-2 oligomers but not by a mutant PGAM-M MEF-2 oligomer. These observations define the PGAM-M enhancer as the only cardiac- and skeletal-muscle-specific enhancer characterized thus far that is mainly activated through MEF-2.


Asunto(s)
Bisfosfoglicerato Mutasa/genética , Proteínas de Unión al ADN/metabolismo , Elementos de Facilitación Genéticos , Músculos/enzimología , Miocardio/enzimología , Factores de Transcripción/metabolismo , Transcripción Genética , Animales , Secuencia de Bases , Bisfosfoglicerato Mutasa/metabolismo , Línea Celular , Clonación Molecular , ADN , Regulación Enzimológica de la Expresión Génica , Humanos , Factores de Transcripción MEF2 , Ratones , Datos de Secuencia Molecular , Proteínas Musculares/genética , Proteínas Musculares/metabolismo , Músculos/citología , Mutagénesis , Proteína MioD , Miocardio/citología , Factores Reguladores Miogénicos , Proteínas Recombinantes de Fusión/genética , Proteínas Recombinantes de Fusión/metabolismo , Transfección
6.
J Neuroimmunol ; 62(1): 103-12, 1995 Oct.
Artículo en Inglés | MEDLINE | ID: mdl-7499486

RESUMEN

A cytokine-mediated excessive increase in nitric oxide (NO) by macrophages or glial cells via an inducible isoform of NO synthase (iNOS) has been proposed to play an important role in demyelinating diseases. To further investigate the role of iNOS in demyelination, experimental allergic encephalomyelitis (EAE), a known animal model of multiple sclerosis (MS) in mice, was chosen in this study. A semiquantitative reverse transcriptase-polymerase chain reaction (RT/PCR) analysis revealed an increase in the mRNA levels of iNOS and cytokines known to induce iNOS or inflammatory cytokines (interleukin (IL)-1 alpha, IL-1 beta, IL-2, IL-6, interferon (IFN)-gamma, tumor necrosis factor (TNF)-alpha and TNF-beta) in the spinal cord corresponding to the severity of the disease without significant change in the mRNA levels of immunoregulatory cytokines (IL-4, IL-10 and transforming growth factor (TGF)-beta) during the course of EAE. An immunohistochemical examination of the spinal cord using an iNOS-specific antibody showed iNOS-positive cells to be mainly inflammatory cells with a higher frequency of iNOS-positive cells at the peak of EAE than in the early phase. These iNOS-positive cells at the peak appeared to be composed of infiltrating macrophages and most of them were located in the necrotic area. These results suggested that cytokine-induced excessive NO via iNOS by macrophages caused tissue damage in the central nervous system in EAE.


Asunto(s)
Encefalomielitis Autoinmune Experimental/inmunología , Isoenzimas/genética , Óxido Nítrico Sintasa/genética , Animales , Secuencia de Bases , Citocinas/inmunología , Encefalomielitis Autoinmune Experimental/enzimología , Encefalomielitis Autoinmune Experimental/genética , Femenino , Expresión Génica/inmunología , Inmunohistoquímica , Isoenzimas/inmunología , Ratones , Ratones Endogámicos , Datos de Secuencia Molecular , Proteína Básica de Mielina/inmunología , Óxido Nítrico Sintasa/inmunología , Reacción en Cadena de la Polimerasa , Médula Espinal/enzimología , Médula Espinal/inmunología , Médula Espinal/patología
7.
J Endocrinol ; 159(2): 323-30, 1998 Nov.
Artículo en Inglés | MEDLINE | ID: mdl-9795374

RESUMEN

Cytokines and steroid hormones use different sets of signal transduction pathways, which seem to be unrelated. Interleukin-6 (IL-6) uses JAK tyrosine kinase and STAT (signal transducer and activator of transcription) transcription factor. Glucocorticoid binds glucocorticoid receptor (GR), which is a member of the steroid receptor superfamily. We have studied the crosstalk between the IL-6-JAK-STAT and glucocorticoid-nuclear receptor pathways. IL-6 and glucocorticoid synergistically activated the IL-6 response element on the rat alpha2-macroglobulin promoter (APRE)-driven luciferase gene. The exogenous expression of GR enhanced the synergism. The exogenous expression of dominant negative STAT3 completely abolished the IL-6 plus glucocorticoid-induced activation of the APRE-luciferase gene. Tyrosine phosphorylation of STAT3 stimulated by IL-6 alone was not different from that by IL-6 plus glucocorticoid. The protein level of STAT3 was also not increased by glucocorticoid stimulation. The time course of STAT3 tyrosine phosphorylation by IL-6 plus glucocorticoid was not different from that by IL-6 alone. The synergism was studied on the two other IL-6 response elements, the junB promoter (JRE-IL-6) and the interferon regulatory factor-1 (IRF-1) promoter (IRF-GAS) which could be activated by STAT3. The synergistic activation by glucocorticoid on the IL-6-activated JRE-IL-6 and the IRF-GAS-driven luciferase gene was not detected. Glucocorticoid did not change the mobility of IL-6-induced APRE-binding proteins in a gel shift assay. These results suggest that the synergism was through the GR and STAT3, and the coactivation pathway which was specific for APRE was the target of glucocorticoid.


Asunto(s)
Glucocorticoides/metabolismo , Interleucina-6/metabolismo , Luciferasas/genética , Transducción de Señal , Proteínas de Unión al ADN/genética , Proteínas de Unión al ADN/metabolismo , Dexametasona/metabolismo , Sinergismo Farmacológico , Expresión Génica , Humanos , Factor 1 Regulador del Interferón , Janus Quinasa 1 , Fosfoproteínas/genética , Fosforilación , Regiones Promotoras Genéticas , Proteínas Tirosina Quinasas/metabolismo , Proteínas Proto-Oncogénicas c-jun/genética , Receptores de Glucocorticoides/metabolismo , Factor de Transcripción STAT3 , Transactivadores/metabolismo , Células Tumorales Cultivadas , Tirosina/metabolismo
8.
J Neurol Sci ; 177(2): 154-6, 2000 Aug 15.
Artículo en Inglés | MEDLINE | ID: mdl-10980313

RESUMEN

We report a patient with HTLV-I-associated myelopathy (HAM), who developed symptoms of myelopathy 4 years after cadaveric renal transplantation. Since he was seronegative before the transplantation, it is suggested that HTLV-I infection was transmitted via renal graft transplantation. He has been treated with immunosuppressive agents such as cyclosporin A (CsA), mycophenolate mofetil (MMF), and prednisolone (PSL) to prevent graft rejection. This case suggested that these immunosuppressive agents are poorly effective in suppressing either the onset or progression of HAM/TSP.


Asunto(s)
Trasplante de Riñón/efectos adversos , Paraparesia Espástica Tropical/patología , Humanos , Inmunosupresores/efectos adversos , Fallo Renal Crónico/cirugía , Masculino , Persona de Mediana Edad , Paraparesia Espástica Tropical/transmisión
9.
J Org Chem ; 65(11): 3326-33, 2000 Jun 02.
Artículo en Inglés | MEDLINE | ID: mdl-10843613

RESUMEN

Novel C(2)-symmetric chiral bisoxazoline ligands 1 were easily prepared from enantiomerically pure 2-amino alcohols and achiral 2, 2'-biphenyldicarboxylic acid via the corresponding amide and mesylate as intermediates. Since these ligands bear only two ortho-substituents on the biphenyl backbone, the biphenyl axis is not fixed, and the two diastereomers of these ligands exist in equilibrium in solution. Interestingly, when the ligands 1 were coordinated with a metal ion, only one of the two possible diastereomer complexes, an (S,aS,S)-complex, can be formed depending on the combination of the ligand and the metal ion. Thus, copper(I) afforded only the (S,aS,S)-complexes with all ligands 1, while zinc(II), palladium(II), and silver(I) afforded the (S,aS, S)-complexes as the sole product only with 1b, which has a bulky tert-butyl group on the oxazoline ring, and a mixture of the two diastereomer complexes with 1a,c,d. The copper(I)-catalyzed asymmetric cyclopropanation of styrene with diazoacetate proceeded successfully with these ligands and good to excellent enantioselectivities were afforded.

10.
J Gastroenterol ; 33(3): 419-23, 1998 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-9658324

RESUMEN

Two patients with acute hepatitis B with suggested sexual transmission of hepatitis G virus (HGV) are reported. A total of 18 patients with community acquired acute hepatitis B were analyzed in this study. Two of the 18 patients (patients 1 and 2) were positive for serum HGV RNA at the initial consultation. Both patients had had sexual contact with prostitutes several weeks before the onset of acute hepatitis, and hepatitis B virus (HBV) was suggested to be infected through the sexual contacts. These patients showed no other history of exposure to possible transmission routes for blood-borne hepatitis viruses. Patient 1 was diagnosed as with acute HGV infection because the antibody to HGV envelope-2 protein seroconverted to positive during the course of acute hepatitis. HGV RNA was negative in a serum sample collected from patient 2 before the onset of acute hepatitis, also suggesting acute HGV infection. These results indicate that in patients 1 and 2 HGV was infected along with HBV through sexual contact. The clinical manifestations of acute hepatitis in the two patients with HGV co-infection did not differ from those in the 16 patients with HBV infection alone.


Asunto(s)
Flaviviridae , Hepatitis B/complicaciones , Hepatitis Viral Humana/transmisión , Hepatitis Viral Humana/virología , Enfermedades de Transmisión Sexual/virología , Adolescente , Adulto , Anciano , Niño , Preescolar , Estudios de Cohortes , Infecciones Comunitarias Adquiridas/complicaciones , Femenino , Flaviviridae/genética , Flaviviridae/inmunología , Anticuerpos Antihepatitis/sangre , Hepatitis B/inmunología , Hepatitis Viral Humana/complicaciones , Hepatitis Viral Humana/inmunología , Humanos , Masculino , Persona de Mediana Edad , ARN Viral/sangre
11.
Clin Neuropathol ; 20(6): 272-5, 2001.
Artículo en Inglés | MEDLINE | ID: mdl-11758783

RESUMEN

We report a very rare case of occult leptomeningeal carcinomatosis (LC) in whom repeated cytological examination did not show malignant cells in cerebrospinal fluid (CSF) and the primary focus was not discovered by extensive survey. The patient presented with ophthalmoplegia, ataxia and areflexia mimicking Miller Fisher syndrome (MFS) at the initial stage, and later, the clinical profile and laboratory findings including CSF examination simulated tuberculous meningitis. Postmortem autopsy disclosed metastatic signet-ring cell carcinoma infiltrating into cranial nerves and leptomeninges. We would like to emphasize that LC sometimes shows symptoms and signs similar to MFS or tuberculous meningitis.


Asunto(s)
Ataxia/etiología , Carcinoma de Células en Anillo de Sello/secundario , Neoplasias Meníngeas/secundario , Neoplasias Primarias Desconocidas/patología , Oftalmoplejía/etiología , Reflejo Anormal/fisiología , Anciano , Carcinoma de Células en Anillo de Sello/patología , Ventrículos Cerebrales/patología , Diagnóstico Diferencial , Humanos , Nervio Hipogloso/patología , Imagen por Resonancia Magnética , Masculino , Neoplasias Meníngeas/patología , Meninges/patología
12.
Intern Med ; 40(11): 1140-3, 2001 Nov.
Artículo en Inglés | MEDLINE | ID: mdl-11757772

RESUMEN

A 66-year-old man developed progressive painful dysesthesia in his hands and feet over 3 months. His vibration sense was impaired and sensory nerve action potentials of the limbs were not evoked. Biopsy of the peroneal nerve revealed sensory neuropathy. Positive anti-Hu antibody facilitated delineation of a right hilar mass and a metastatic lymph node in thoracic CT scan. He was diagnosed as small cell lung cancer associated with paraneoplastic sensory neuropathy. A complete response was achieved through chemotherapy (carboplatin and etoposide) and subsequent radiation therapy. Notably, his neurological conditions, although not changed during the hospitalization, gradually improved afterwards.


Asunto(s)
Autoanticuerpos/sangre , Carcinoma de Células Pequeñas/tratamiento farmacológico , Carcinoma de Células Pequeñas/radioterapia , Neoplasias Pulmonares/tratamiento farmacológico , Neoplasias Pulmonares/radioterapia , Polineuropatía Paraneoplásica/inmunología , Anciano , Protocolos de Quimioterapia Combinada Antineoplásica/uso terapéutico , Carboplatino/administración & dosificación , Carcinoma de Células Pequeñas/inmunología , Etopósido/administración & dosificación , Humanos , Neoplasias Pulmonares/inmunología , Masculino , Resultado del Tratamiento
13.
Rinsho Shinkeigaku ; 40(9): 926-8, 2000 Sep.
Artículo en Japonés | MEDLINE | ID: mdl-11257791

RESUMEN

We report a patient with Gerstmann-Sträussler-Scheinker syndrome (GSS102) who developed optic atrophy. He had been complaining of slowly progressive postural unsteadiness and pain in both legs for 3 years. Visual acuity subacutely worsened in the last half year. His father and two aunts, who already died, had been diagnosed to have dementia. It is uncertain whether they had optic atrophy or not. He was alert but apathetic. Neurological examination revealed cerebellar ataxia, painful dysesthesia and loss of deep tendon reflexes in the lower limbs. Fundoscopic examination revealed bilateral optic atrophy without retinal degeneration, which has never been reported in GSS. A brain MRI showed mild atrophy of cerebellar hemispheres without signal abnormalities of optic nerves. DNA analysis of prion gene revealed point mutation at codon 102 (P102L), which was relatively common mutation in GSS. Other mutations were not found. Only two patients of Creutzfeldt-Jakob disease with optic atrophy have been reported. This case seems to be important to investigate why optic tracts are generally spared in prion disease.


Asunto(s)
Enfermedad de Gerstmann-Straussler-Scheinker/genética , Atrofia Óptica/etiología , Mutación Puntual , Priones/genética , Enfermedad de Gerstmann-Straussler-Scheinker/complicaciones , Humanos , Masculino , Persona de Mediana Edad
14.
Nihon Rinsho ; 52(11): 2946-51, 1994 Nov.
Artículo en Japonés | MEDLINE | ID: mdl-7996692

RESUMEN

Successful transfer of multiple sclerosis (MS) into severe combined immunodeficient (SCID) mice by intracisternal injection of cerebrospinal fluid (CSF) cells from six of 15 patients at exacerbation has been reported. The relative expression of mRNA for cytokines in demyelinated lesions from SCID-hu-MS by a semiquantitative reverse transcriptase-polymerase chain reaction analysis revealed marked increase in expression of mRNAs for TNF-alpha and IL-6. This result raised the possibility that these cytokines played an important role in the demyelinating process in SCID-hu-MS. To test whether or not an antibody (Ab) to murine TNF-alpha has a preventive effect on transfer of MS into SCID mice, intracisternal injection of CSF cells from two patients at exacerbation together with anti-murine TNF-alpha Ab was performed, and we observed effective prevention of transfer. In addition, we also found an Ab to human CD3 (OKT3) to have a preventive effect. The former result suggests that control of TNF-alpha may be effective in treatment of MS and the latter suggests that MS is a T cell-mediated disease.


Asunto(s)
Esclerosis Múltiple/inmunología , Linfocitos T/inmunología , Adolescente , Adulto , Animales , Femenino , Humanos , Masculino , Ratones , Ratones SCID , Persona de Mediana Edad , Esclerosis Múltiple/terapia , Linfocitos T/trasplante
15.
Nihon Rinsho ; 55(3): 604-8, 1997 Mar.
Artículo en Japonés | MEDLINE | ID: mdl-9086766

RESUMEN

Hepatitis G virus(HGV) is a new RNA virus that is most closely related to members of the Flaviviridae family. HGV RNA was found in only a low percentage of patients with either acute or chronic non-A-E hepatitis. HGV appears to co-infect or superinfect in 10-15% of HCV infections, and in 5-15% of HBV infections. The prevalence of HGV infection(0.8%) among voluntary blood donor in Japan is similar to that for HCV infection. A history of blood transfusion was obtained in 50-60% of HGV RNA positive subjects. HGV was also detected in about 10% of hemodialysis patients. Finally, isolated HGV infection appears to have a low disease burden.


Asunto(s)
Flaviviridae , Hepatitis Viral Humana/epidemiología , Biomarcadores/sangre , Donantes de Sangre , Hepatitis B/complicaciones , Hepatitis C/complicaciones , Hepatitis Viral Humana/complicaciones , Hepatitis Viral Humana/transmisión , Humanos , Japón/epidemiología , Reacción en Cadena de la Polimerasa , Prevalencia , ARN Viral/sangre , Diálisis Renal/efectos adversos , Reacción a la Transfusión
16.
J Oleo Sci ; 62(3): 123-32, 2013.
Artículo en Inglés | MEDLINE | ID: mdl-23470439

RESUMEN

Hydrogen bonding between surfactant molecules plays an important role in self-assembly formation. For long alkyl chain amine oxide surfactants, the specific protonation degree dependence of some solution properties has been considered to be due to hydrogen bonding between protonated and deprotonated species. In addition to this type of hydrogen bonding, we introduced a pyridyl group into an alkylamine oxide molecule as a new hydrogen-bonding site. The pyridyl group has three different structural isomers based on the position of the substituent. An amine oxide group in pyridylamine oxides was preferentially protonated. In addition, protonation of the pyridyl group revealed a pronounced substituent position effect on the critical micelle concentration, micellar size, and solubilization of oil-soluble dye into micelles. The intermolecular or intramolecular hydrogen bond formation could be controlled by altering the substituent position.


Asunto(s)
Aminas/química , Óxidos/química , Piridinas/química , Tensoactivos/química , Enlace de Hidrógeno , Estructura Molecular , Protones , Soluciones
17.
Nihon Rinsho ; 57 Suppl: 27-9, 1999 Dec.
Artículo en Japonés | MEDLINE | ID: mdl-10778053
19.
Cytokine ; 36(1-2): 69-74, 2006 Oct.
Artículo en Inglés | MEDLINE | ID: mdl-17161613

RESUMEN

In order to predict the clinical benefit of interferon-beta (IFN-beta) to patients with multiple sclerosis (MS), the following markers were investigated; (1) chronological change of cytokines (IFN-gamma, TNF-alpha, IL-6, IL-10, and TGF-beta) after administration of IFN-beta, (2) untoward effects of IFN-beta such as headache and arthralgia, (3) backgrounds of the patients such as age and relapse rate, (4) efficacy of IFN-beta therapy assessed by the change of relapse rate and progression of disability. Chronological blood sampling was performed 0, 10, and 24 h after injection of IFN-beta. The increase of serum IL-6 level in response to IFN-beta administration was associated with headache, arthralgia, relapse rate before treatment, and disability score at the initiation of the therapy. Significant association of change of serum TNF-alpha with age and headache was also observed. The important finding in this study was that patients with a transient increase in IL-6 in response to IFN-beta showed a slow disease progression. This result suggests that this transient increase in the serum IL-6 predicts favorable response to IFN-beta treatment.


Asunto(s)
Interferón beta/uso terapéutico , Interleucina-6/sangre , Esclerosis Múltiple/sangre , Esclerosis Múltiple/tratamiento farmacológico , Adulto , Envejecimiento/sangre , Personas con Discapacidad , Progresión de la Enfermedad , Femenino , Humanos , Inyecciones , Interferón beta/administración & dosificación , Masculino , Esclerosis Múltiple/patología , Factor de Necrosis Tumoral alfa/sangre
20.
Dev Neurosci ; 23(4-5): 356-63, 2001.
Artículo en Inglés | MEDLINE | ID: mdl-11756751

RESUMEN

The proliferation of oligodendrocyte precursors is closely regulated in the developing vertebrate CNS. In previous studies we have demonstrated a cell-type-specific density-dependent feedback mechanism that contributes to the control of oligodendrocyte progenitor cell clonal expansion in vitro. Here we demonstrate a density-dependent reduction in the proliferation of oligodendrocyte precursors. This inhibition of proliferation is correlated with increases in expression levels of the cell cycle inhibitor p27(Kip1), reductions in the expression of cyclin A and changes in the relative phosphorylation levels of Rb. These changes are reversible on replating at low density suggesting that additional signals are required for terminal oligodendrocyte differentiation.


Asunto(s)
Proteínas de Ciclo Celular/metabolismo , División Celular/fisiología , Sistema Nervioso Central/crecimiento & desarrollo , Regulación del Desarrollo de la Expresión Génica/fisiología , Oligodendroglía/metabolismo , Células Madre/metabolismo , Animales , Animales Recién Nacidos , Células Cultivadas , Sistema Nervioso Central/citología , Sistema Nervioso Central/metabolismo , Corteza Cerebral , Células Clonales/citología , Células Clonales/metabolismo , Ciclina A/metabolismo , Inhibidor p27 de las Quinasas Dependientes de la Ciclina , Regulación hacia Abajo/fisiología , Oligodendroglía/citología , Ratas , Células Madre/citología , Proteínas Supresoras de Tumor/metabolismo , Regulación hacia Arriba/fisiología
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