RESUMEN
Growth retardation resulting in short stature is a major concern for parents and due to its great variety of causes, a complex diagnostic challenge for clinicians. A major locus involved in linear growth has been implicated within the pseudoautosomal region (PAR1) of the human sex chromosomes. We have determined an interval of 170 kb of DNA within PAR1 which was deleted in 36 individuals with short stature and different rearrangements on Xp22 or Yp11.3. This deletion was not detected in any of the relatives with normal stature or in a further 30 individuals with rearrangements on Xp22 or Yp11.3 with normal height. We have isolated a homeobox-containing gene (SHOX) from this region, which has at least two alternatively spliced forms, encoding proteins with different patterns of expression. We also identified one functionally significant SHOX mutation by screening 91 individuals with idiopathic short stature. Our data suggest an involvement of SHOX in idiopathic growth retardation and in the short stature phenotype of Turner syndrome patients.
Asunto(s)
Estatura/genética , Eliminación de Gen , Genes Homeobox , Trastornos del Crecimiento/genética , Proteínas de Homeodominio/genética , Síndrome de Turner/genética , Adolescente , Secuencia de Aminoácidos , Secuencia de Bases , Niño , Mapeo Cromosómico , Clonación Molecular , Femenino , Proteínas de Homeodominio/metabolismo , Humanos , Lactante , Recién Nacido , Masculino , Datos de Secuencia Molecular , Mutación , Linaje , Embarazo , Análisis de Secuencia de ADN , Proteína de la Caja Homeótica de Baja Estatura , Distribución Tisular , Cromosoma X , Cromosoma YRESUMEN
Human-chimpanzee comparative genome research is essential for narrowing down genetic changes involved in the acquisition of unique human features, such as highly developed cognitive functions, bipedalism or the use of complex language. Here, we report the high-quality DNA sequence of 33.3 megabases of chimpanzee chromosome 22. By comparing the whole sequence with the human counterpart, chromosome 21, we found that 1.44% of the chromosome consists of single-base substitutions in addition to nearly 68,000 insertions or deletions. These differences are sufficient to generate changes in most of the proteins. Indeed, 83% of the 231 coding sequences, including functionally important genes, show differences at the amino acid sequence level. Furthermore, we demonstrate different expansion of particular subfamilies of retrotransposons between the lineages, suggesting different impacts of retrotranspositions on human and chimpanzee evolution. The genomic changes after speciation and their biological consequences seem more complex than originally hypothesized.
Asunto(s)
Cromosomas de los Mamíferos/genética , Evolución Molecular , Pan troglodytes/genética , Mapeo Físico de Cromosoma , Animales , Cromosomas Humanos Par 21/genética , Perfilación de la Expresión Génica , Genes/genética , Genómica , Humanos , Mutagénesis/genética , Filogenia , Polimorfismo de Nucleótido Simple/genética , Regiones Promotoras Genéticas/genética , Secuencias Repetitivas de Ácidos Nucleicos/genética , Retroelementos/genética , Análisis de Secuencia de ADNRESUMEN
Listeria monocytogenes is a food-borne pathogen with a high mortality rate that has also emerged as a paradigm for intracellular parasitism. We present and compare the genome sequences of L. monocytogenes (2,944,528 base pairs) and a nonpathogenic species, L. innocua (3,011,209 base pairs). We found a large number of predicted genes encoding surface and secreted proteins, transporters, and transcriptional regulators, consistent with the ability of both species to adapt to diverse environments. The presence of 270 L. monocytogenes and 149 L. innocua strain-specific genes (clustered in 100 and 63 islets, respectively) suggests that virulence in Listeria results from multiple gene acquisition and deletion events.
Asunto(s)
Proteínas Bacterianas/genética , Genoma Bacteriano , Listeria monocytogenes/genética , Listeria/genética , Adaptación Fisiológica , Secuencias de Aminoácidos , Bacillus subtilis/genética , Proteínas Bacterianas/química , Proteínas Bacterianas/fisiología , Composición de Base , Proteínas Portadoras/química , Proteínas Portadoras/genética , Cromosomas Bacterianos/genética , ADN Bacteriano/química , ADN Bacteriano/genética , Transferencia de Gen Horizontal , Genes Bacterianos , Genómica , Listeria/química , Listeria/fisiología , Listeria monocytogenes/química , Listeria monocytogenes/patogenicidad , Listeria monocytogenes/fisiología , Proteínas de la Membrana/química , Proteínas de la Membrana/genética , Análisis de Secuencia de ADN , Staphylococcus aureus/genética , Factores de Transcripción/química , Factores de Transcripción/genética , Virulencia/genéticaRESUMEN
BACKGROUND: Myxobacteria have been well established as a potent source for natural products with biological activity. They produce a considerable variety of compounds which represent typical polyketide structures with incorporated amino acids (e.g. the epothilons, the myxothiazols and the myxalamids). Several of these secondary metabolites are effective inhibitors of the electron transport via the respiratory chain and have been widely used. Molecular cloning and characterization of the genes governing the biosynthesis of these structures is of considerable interest, because such information adds to the limited knowledge as to how polyketide synthases (PKSs) and non-ribosomal peptide synthetases (NRPSs) interact and how they might be manipulated in order to form novel antibiotics. RESULTS: A DNA region of approximately 50000 base pairs from Stigmatella aurantiaca Sga15 was sequenced and shown by gene disruption to be involved in myxalamid biosynthesis. Sequence analysis reveals that the myxalamids are formed by a combined PKS/NRPS system. The terminal NRPS MxaA extends the assembled polyketide chain of the myxalamids with alanine. MxaA contains an N-terminal domain with homology to NAD binding proteins, which is responsible during the biogenesis for a novel type of reductive chain release giving rise to the 2-amino-propanol moiety of the myxalamids. The last module of the PKS reveals an unprecedented genetic organization; it is encoded on two genes (mxaB1 and mxaB2), subdividing the domains of one module from each other. A sequence comparison of myxobacterial acyl-transferase domains with known systems from streptomycetes and bacilli reveals that consensus sequences proposed to be specific for methylmalonyl-CoA and malonyl-CoA are not always reliable. CONCLUSIONS: The complete biosynthetic gene cluster of the myxalamid-type electron transport inhibitor from S. aurantiaca Sga15 has been cloned and analyzed. It represents one of the few examples of combined PKS/NRPS systems, the analysis and manipulation of which has the potential to generate novel hybrid structures via combinatorial biosynthesis (e.g. via module-swapping techniques). Additionally, a new type of reductive release from PKS/NRPS systems is described.
Asunto(s)
Genes Bacterianos , Complejos Multienzimáticos/genética , Familia de Multigenes , Péptido Sintasas/genética , Stigmatella aurantiaca/enzimología , Stigmatella aurantiaca/genética , Secuencia de Aminoácidos , Antibacterianos/biosíntesis , Secuencia de Bases , Clonación Molecular , Cartilla de ADN/genética , Modelos Biológicos , Datos de Secuencia Molecular , Polienos/metabolismo , Estructura Terciaria de Proteína , Homología de Secuencia de AminoácidoRESUMEN
The biosynthetic gene cluster of the myxochelin-type iron chelator was cloned from Stigmatella aurantiaca Sg a15 and characterized. This catecholate siderophore was only known from two other myxobacteria. The biosynthetic genes of 2,3-dihydroxybenzoic acid are located in the cluster (mxcC-mxcF). Two molecules of 2, 3-dihydroxybenzoic acid are activated and condensed with lysine in a unique way by a protein homologous to nonribosomal peptide synthetases (MxcG). Inactivation of mxcG, which encodes an adenylation domain for lysine, results in a myxochelin negative mutant unable to grow under iron-limiting conditions. Growth could be restored by adding Fe3+, myxochelin A or B to the medium. Inactivation of mxcD leads to the same phenotype. A new type of reductive release from nonribosomal peptide synthetases of the 2, 3-dihydroxybenzoic acid bis-amide of lysine from MxcG, catalyzed by a protein domain with homology to NAD(P) binding sites, is discussed. The product of a gene, encoding a protein similar to glutamate-1-semialdehyde 2,1-aminomutases (mxcL), is assumed to transaminate the aldehyde that is proposed as an intermediate. Further genes encoding proteins homologous to typical iron utilization and iron uptake polypeptides are reported.
Asunto(s)
Hierro/metabolismo , Lisina/análogos & derivados , Lisina/genética , Regulón/genética , Stigmatella aurantiaca/genética , Stigmatella aurantiaca/metabolismo , Secuencia de Aminoácidos , Transporte Biológico , Cromatografía Líquida de Alta Presión , Secuencia Conservada , Regulación Bacteriana de la Expresión Génica , Hidroxibenzoatos/metabolismo , Transferasas Intramoleculares/genética , Transferasas Intramoleculares/metabolismo , Quelantes del Hierro/metabolismo , Lisina/biosíntesis , Lisina/química , Lisina/metabolismo , Datos de Secuencia Molecular , Familia de Multigenes/genética , Mutación/genética , Operón/genética , Estructura Terciaria de Proteína , Secuencias Reguladoras de Ácidos Nucleicos/genética , Alineación de Secuencia , Stigmatella aurantiaca/enzimologíaRESUMEN
aDNA-analyses are presented for ancient bones, teeth, soft tissues and dried body fluids. The implications and perspectives for Historical Anthropology are discussed. Methods are described on examples of molecular sex determination and kinship analysis. Finally, first amplifications of DNA-sequences from pathogenes of tuberculosis and plague from ancient skeletal material are reported.
Asunto(s)
Antropología Física/métodos , ADN/análisis , Autoanálisis , Líquidos Corporales , Huesos/química , ADN/genética , Femenino , Humanos , Masculino , Reacción en Cadena de la Polimerasa/métodos , Análisis para Determinación del Sexo , Diente/química , Tuberculosis/microbiología , Cromosoma Y , Yersiniosis/microbiología , Yersinia enterocolitica/genética , Yersinia enterocolitica/aislamiento & purificación , Yersinia pestis/genética , Yersinia pestis/aislamiento & purificaciónRESUMEN
The biosynthetic mta gene cluster responsible for myxothiazol formation from the fruiting body forming myxobacterium Stigmatella aurantiaca DW4/3-1 was sequenced and analyzed. Myxothiazol, an inhibitor of the electron transport via the bc(1)-complex of the respiratory chain, is biosynthesized by a unique combination of several polyketide synthases (PKS) and nonribosomal peptide synthetases (NRPS), which are activated by the 4'-phosphopantetheinyl transferase MtaA. Genomic replacement of a fragment of mtaB and insertion of a kanamycin resistance gene into mtaA both impaired myxothiazol synthesis. Genes mtaC and mtaD encode the enzymes for bis-thiazol(ine) formation and chain extension on one pure NRPS (MtaC) and on a unique combination of PKS and NRPS (MtaD). The genes mtaE and mtaF encode PKSs including peptide fragments with homology to methyltransferases. These methyltransferase modules are assumed to be necessary for the formation of the proposed methoxy- and beta-methoxy-acrylate intermediates of myxothiazol biosynthesis. The last gene of the cluster, mtaG, again resembles a NRPS and provides insight into the mechanism of the formation of the terminal amide of myxothiazol. The carbon backbone of an amino acid added to the myxothiazol-acid is assumed to be removed via an unprecedented module with homology to monooxygenases within MtaG.
Asunto(s)
Genes Bacterianos , Familia de Multigenes , Stigmatella/genética , Secuencia de Aminoácidos , Clonación Molecular , Metacrilatos , Metiltransferasas/genética , Datos de Secuencia Molecular , Complejos Multienzimáticos/genética , Péptido Sintasas/genética , Plásmidos , Tiazoles/metabolismoRESUMEN
MTM1 is responsible for X-linked recessive myotubular myopathy, which is a congenital muscle disorder linked to Xq28. MTM1 is highly conserved from yeast to humans. A number of related genes also exist. The MTM1 gene family contains a consensus sequence consisting of the active enzyme site of protein tyrosine phosphatases (PTPs), suggesting that they belong to a new family of PTPs. Database searches revealed homology of myotubularin and all related peptides to the cisplatin resistance-associated alpha protein, which implicates an as yet unknown function. In addition, homology to the Sbf1 protein (SET binding factor 1), involved in the oncogenic transformation of fibroblasts and differentiation of myoblasts, was also evident. We describe 225 kb of genomic sequence containing MTM1 and the related gene, MTMR1, which lies 20 kb distal to MTM1. Although there is only moderate conservation of the exons, the striking similarity in the gene structures indicates that these two genes arose by duplication. Calculations suggest that this event occurred early in evolution long before separation of the human and mouse lineages. So far, mutations have been identified in the coding sequence of only 65% of the patients analyzed, indicating that the remaining mutations may lie in noncoding regions of MTM1 or possibly in MTMR1. Knowledge of the genomic sequence will facilitate mutation analyses of the coding and noncoding sequences of MTM1 and MTMR1.
Asunto(s)
Enfermedades Genéticas Congénitas/genética , Músculos/patología , Proteínas Tirosina Fosfatasas/genética , Cromosoma X/genética , Secuencia de Aminoácidos , Secuencia de Bases , Clonación Molecular , Repeticiones de Dinucleótido/genética , Exones/genética , Duplicación de Gen , Humanos , Intrones/genética , Repeticiones de Microsatélite/genética , Datos de Secuencia Molecular , Proteínas Tirosina Fosfatasas no Receptoras , Alineación de Secuencia , Análisis de Secuencia de ADN , Repeticiones de Trinucleótidos/genéticaRESUMEN
The progress of human and mouse genome sequencing programs presages the possibility of systematic cross-species comparison of the two genomes as a powerful tool for gene and regulatory element identification. As the opportunities to perform comparative sequence analysis emerge, it is important to develop parameters for such analyses and to examine the outcomes of cross-species comparison. Our analysis used gene prediction and a database search of 430 kb of genomic sequence covering the Bpa/Str region of the mouse X chromosome, and 745 kb of genomic sequence from the homologous human X chromosome region. We identified 11 genes in mouse and 13 genes and two pseudogenes in human. In addition, we compared the mouse and human sequences using pairwise alignment and searches for evolutionary conserved regions (ECRs) exceeding a defined threshold of sequence identity. This approach aided the identification of at least four further putative conserved genes in the region. Comparative sequencing revealed that this region is a mosaic in evolutionary terms, with considerably more rearrangement between the two species than realized previously from comparative mapping studies. Surprisingly, this region showed an extremely high LINE and low SINE content, low G+C content, and yet a relatively high gene density, in contrast to the low gene density usually associated with such regions.
Asunto(s)
Proteínas Cromosómicas no Histona , Análisis de Secuencia de ADN , Cromosoma X/genética , 3-Hidroxiesteroide Deshidrogenasas/genética , Secuencia de Aminoácidos , Animales , Antígenos de Neoplasias/genética , Antígenos de Neoplasias/aislamiento & purificación , Proteínas de Unión al Calcio/genética , Proteínas del Citoesqueleto , Proteínas de Unión al ADN/genética , Biblioteca Genómica , Humanos , Proteínas con Dominio LIM , Antígenos Específicos del Melanoma , Ratones , Datos de Secuencia Molecular , Familia de Multigenes , Proteínas de Neoplasias/genética , Proteínas Nucleares/genética , Homología de Secuencia de Ácido Nucleico , Dedos de Zinc/genéticaRESUMEN
Chromosome 21 is the smallest human autosome. An extra copy of chromosome 21 causes Down syndrome, the most frequent genetic cause of significant mental retardation, which affects up to 1 in 700 live births. Several anonymous loci for monogenic disorders and predispositions for common complex disorders have also been mapped to this chromosome, and loss of heterozygosity has been observed in regions associated with solid tumours. Here we report the sequence and gene catalogue of the long arm of chromosome 21. We have sequenced 33,546,361 base pairs (bp) of DNA with very high accuracy, the largest contig being 25,491,867 bp. Only three small clone gaps and seven sequencing gaps remain, comprising about 100 kilobases. Thus, we achieved 99.7% coverage of 21q. We also sequenced 281,116 bp from the short arm. The structural features identified include duplications that are probably involved in chromosomal abnormalities and repeat structures in the telomeric and pericentromeric regions. Analysis of the chromosome revealed 127 known genes, 98 predicted genes and 59 pseudogenes.