RESUMEN
A phage (PhiOT8) isolated on Serratia sp. ATCC 39006 was shown to be flagellum-dependent, and to mediate generalized transduction with high efficiency (up to 10(-4) transductants per p.f.u.). PhiOT8 was shown to have a broad host range because it also infected a strain of Pantoea agglomerans isolated from the rhizosphere. Transduction of plasmid-borne antibiotic resistance between the two bacterial genera was demonstrated, consistent with purported ecological roles of phages in dissemination of genes between bacterial genera. Serratia sp. ATCC 39006 and P. agglomerans produce a number of interesting secondary metabolites that have potential applications in cancer therapy and biocontrol of fungal infections. PhiOT8 has utility as a powerful functional genomics tool in these bacteria.
Asunto(s)
Bacteriófagos/fisiología , Flagelos/fisiología , Pantoea/virología , Serratia/virología , Transducción Genética , Bacteriófagos/genética , Bacteriófagos/aislamiento & purificación , Bacteriófagos/ultraestructura , ADN Viral/genética , Mutagénesis , Pantoea/genética , Serratia/genéticaRESUMEN
Arabidopsis cDNAs encoding ATJ11, the smallest known J-domain protein, have been isolated and characterized. The precursor protein of 161 amino acid residues was synthesized in vitro and imported by isolated pea chloroplasts where it was localized to the stroma and cleaved to a mature protein of 125 amino acid residues. The mature protein consists of an 80 amino acid J-domain, and N- and C-terminal extensions of 24 and 21 amino acid residues, respectively, which show no similarity to regions in other DnaJ-related proteins. ATJ11 produced in Escherichia coli stimulated the weak ATPase activity of E. coli DnaK, but was unable to stimulate refolding of firefly luciferase by DnaK, and inhibited refolding by DnaK, DnaJ and GrpE. ATJ11 is encoded by a single-copy gene on chromosome 4, and is expressed in all plant organs examined. A paralogue of ATJ11, showing 72% identity, is encoded in a 4.5 Mb duplication of chromosome 4 on chromosome 2. These proteins represent a novel class of J-domain proteins.
Asunto(s)
Proteínas de Arabidopsis , Arabidopsis/genética , Proteínas de Escherichia coli , Chaperonas Moleculares/genética , Proteínas de Plantas/genética , Plastidios/metabolismo , Adenosina Trifosfatasas/efectos de los fármacos , Adenosina Trifosfatasas/metabolismo , Secuencia de Aminoácidos , Arabidopsis/metabolismo , Sitios de Unión , Transporte Biológico , Northern Blotting , Southern Blotting , Cloroplastos/metabolismo , Mapeo Cromosómico , ADN Complementario/química , ADN Complementario/genética , ADN Complementario/aislamiento & purificación , ADN de Plantas/genética , Proteínas HSP70 de Choque Térmico/efectos de los fármacos , Proteínas HSP70 de Choque Térmico/metabolismo , Luciferasas/química , Luciferasas/efectos de los fármacos , Metaloendopeptidasas/metabolismo , Chaperonas Moleculares/metabolismo , Datos de Secuencia Molecular , Pisum sativum/metabolismo , Proteínas de Plantas/metabolismo , Proteínas de Plantas/farmacología , Desnaturalización Proteica , Pliegue de Proteína , Precursores de Proteínas/metabolismo , ARN de Planta/genética , ARN de Planta/metabolismo , Alineación de Secuencia , Análisis de Secuencia de ADN , Homología de Secuencia de Aminoácido , Especificidad de la Especie , Distribución TisularRESUMEN
Evaluation of the accuracy of computer interpretation of 2019 standard 12-lead ECG's has been carried out. In 762 records defined as normal by the cardiologist the computer agreed in 41%, found 49% with minor "abnormalities" and read 10% as having gross abnormalities.There were 1257 abnormal records. The computer read 43 of these as normal including five with infarction and three with LVH. Agreement in the various abnormal categories ranged from 31% to 85% and even after review disagreement remained in 33% of cases.