1.
Chem Commun (Camb)
; 52(68): 10400-2, 2016 Aug 16.
Artículo
en Inglés
| MEDLINE
| ID: mdl-27481332
RESUMEN
A mitochondria-targeting ratiometric probe was designed for γ-glutamyltranspeptidase (γGT). Mechanistic study by HPLC and an inhibitor assay showed that the probe underwent γGT-mediated amide-to-amine transformation and induced a ratiometric fluorescence response in cellular mitochondria. Further application was successful for the detection of cancerous colons in mice.
Asunto(s)
Neoplasias del Colon/diagnóstico por imagen , Colorantes Fluorescentes/química , Glutamina/análogos & derivados , Glutamina/química , Indoles/química , Mitocondrias/metabolismo , gamma-Glutamiltransferasa/análisis , Animales , Fluorescencia , Colorantes Fluorescentes/síntesis química , Colorantes Fluorescentes/metabolismo , Glutamina/síntesis química , Glutamina/metabolismo , Células HCT116 , Humanos , Hidrólisis , Indoles/síntesis química , Indoles/metabolismo , Límite de Detección , Ratones , gamma-Glutamiltransferasa/metabolismo
2.
Chem Commun (Camb)
; 46(48): 9197-9, 2010 Dec 28.
Artículo
en Inglés
| MEDLINE
| ID: mdl-21042601
RESUMEN
An activated Michael acceptor type of probe by an intramolecular hydrogen bond has shown a selective fluorescence turn-on response to cyanide through a conjugated addition of the nucleophilic anion to the enone probe with a 1300-fold increase in its fluorescence intensity.