The removal of thermo-tolerant coliform bacteria by immobilized waste stabilization pond algae.

This study investigated the potential of laboratory- scale columns of immobilized micro-algae to disinfect effluents using thermo-tolerant coliforms (TTC) as a model system. Cells of a Chlorella species isolated from a waste stabilization pond complex in Northeast Brazil were immobilized in calcium alginate, packed into glass columns and incubated in contact with TTC suspensions for up to 24 hours. Five to six log removals of TTC were achieved in 6 hours and 11 log removals in 12 hours contact time. The results were similar under artificial light and shaded sunlight. However little or no TTC removal occurred in the light in columns of alginate beads without immobilized algae present or when the immobilized algae were incubated in the dark suggesting that the presence of both algae and light were necessary for TTC decay. There was a positive correlation between K(b) values for TTC and increasing pH in the effluent from the immobilized algal columns within the range pH 7.2 and 8.9. The potential of immobilized algal technology for wastewater disinfection may warrant further investigation.

Thermo-tolerant coliform bacteria decay rates in a full scale waste stabilization pond system in northeast Brazil.

This paper presents the results for thermo-tolerant coliform (TTC) decay rates (K(b)) in a full scale WSP system located in Natal-RN, northeast Brazil. The series comprises a primary facultative pond (2 m deep), followed by two maturation ponds (1.5 m deep) giving a total area of 11 ha. The influent sewage and the pond effluents were monitored weekly during a seven month period. The results showed that the K(b) values predicted by the Marais equation assuming a hydraulic regime of complete mixing overestimated TTC die-off rates. The K(b) value adopted in the project design was 6.20 d(-1) but the mean value found for the WSP system during the monitoring programme was only 0.85 d(-1). This value is low compared to the values cited in the literature for shallow ponds (<1.25 m deep) but similar to values for deeper ponds. The sub optimal TTC removal rate in this WSP system may be caused by the adoption of too high a K(b) value at the design stage and the negative influence of high wind conditions on the mixing regime in the water columns of the ponds. Thus values for K(b) adopted at the design stage of WSP systems should be coherent with the hydraulic flow model, the type of pond, pond depth, and with the surface organic loading.

Baffled primary facultative ponds with inlets and outlets set at different levels treating domestic wastewater in northeast Brazil.

This study evaluates the performance of four 2.3 m deep pilot-scale, independently loaded, primary facultative ponds treating predominantly domestic sewage in northeast Brazil. The ponds contained longitudinal baffles giving different length to width ratios from 3.55 to 32.4. The ponds had mean hydraulic retention times of ~15 days, and mean surface organic loadings of 330 kg BOD(5).ha.d(-1) during the first experimental phase and 375 kg BOD(5).ha.d(-1) in the second. The vertical inlets and outlets pipes were positioned at 1.8 m and 5 cm respectively below the pond surface in the first phase and at 50 cm and 1.8 m respectively in the second. All the ponds functioned as efficient primary facultative ponds but statistical analysis demonstrated no differences in effluent quality for most of the parameters measured for the various configurations of baffles and inlet and outlet depths. All behaved similarly to the unbaffled pond. The only exceptions were suspended solids and chlorophyll a concentrations which tended to be lower for all combinations of baffles with the outlets set 1.8 m below the surface. This study suggested that the longitudinal baffling of primary facultative ponds when using vertical inlets and outlets may well not significantly improve pond performance.

The removal of ammonia from sanitary landfill leachate using a series of shallow waste stabilization ponds.

This study evaluated the efficiency of a shallow (0.5 m deep) waste stabilization pond series to remove high concentrations of ammonia from sanitary landfill leachate. The pond system was located at EXTRABES, Campina Grande, Paraiba, Northeast Brazil. The pond series was fed with sanitary landfill leachate transported by road tanker to the experimental site from the sanitary landfill of the City of Joao Pessoa, Paraiba. The ammoniacal-N surface loading on the first pond of the series was equivalent to 364 kg ha(-1) d(-1) and the COD surface loading equivalent to 3,690 kg ha(-1) d(-1). The maximum mean ammonia removal efficiency was 99.5% achieved by the third pond in the series which had an effluent concentration of 5.3 mg L(-1) ammoniacal-N for an accumulative HRT of 39.5 days. The removal process was mainly attributed to ammonia volatilization (stripping) from the pond surfaces as a result of high surface pH values and water temperatures of 22-26°C. Shallow pond systems would appear to be a promising technology for stripping ammonia from landfill leachate under tropical conditions.

Implications for physical design: The effect of depth on the performance of waste stabilization ponds.

Studies on experimental primary facultative ponds showed that varying the depth from 1.25 m to 2.3 m had no effect on the rates of BOD removal. In contrast k values for FC removal rates were higher in the shallower (1.25 m) facultative ponds. The risk of odour release via H2S production was higher in the 2.2 m ponds than the 1.25 m ponds and NH3 removal was much better in the 1.25 m facultative ponds. A comparison of the efficiency of shallow 5-pond series (1.0 m and 0.61 m deep) with a 2.2 m deep series showed that the shallow systems were more efficient at FC removal, but the deeper series actually saved land area for the same FC final effluent quality under tropical conditions. However, efficient nutrient removal (N and P) only occurred in the shallow series and effluent standards for nutrient concentrations are unlikely to be met by 2.2 m deep 5-pond series in contrast to the norms for pathogen removal.

Variations in BOD, algal biomass and organic matter biodegradation constants in a wind-mixed tropical facultative waste stabilization pond.

This study considered the impact of wind mixing on the efficiency of BOD removal and the first order biodegradation constant for organic matter in a primary facultative pond. Wind speeds of 1-4 m/s blowing from the effluent end of the pond towards the influent created surface-water flows of up to 0.94 m/s as determined by orange and coconut drogues moving in the opposite direction to the bulk hydraulic flow of 0.217 m/s. This was sufficient to cause mixing of the water column resulting in loss of stratification in terms of chlorophyll a, temperature and dissolved oxygen. BOD and chlorophyll a concentrations were spatially and temporally homogeneous throughout this large pond. BOD removal efficiency was only 50.30% as opposed to a projected value of 79% despite an acceptable surface organic loading of 350 kgBOD5/ha/d and an actual k value for BOD removal using influent sewage samples of 0.29 d-1 close to the projected value of 0.30 d-1. It would seem that wind mixing reduced pond efficiency by destroying stratification and thus reducing the microbial activity necessary to consume organic material. Mixing also increased the mean chlorophyll a concentration compared to stratified facultative ponds receiving similar loads and non-motile algae dominated the water column.

Marine-based waste stabilisation ponds: an evaluation of the hydraulic viability.

Marine-Based Waste Stabilisation Ponds, formed by floating boom/skirt curtain enclosures anchored in sheltered waters, have recently been proposed for provision of low-cost effluent treatment facilities where land availability is limited in rapidly expanding coastal cities in the Third World. This paper outlines findings from a viability assessment, which drew on a preliminary site evaluation and baseline environmental survey (for a site in sheltered waters in the Far East), laboratory studies involving scale model tests in wave/wind/current flumes, and a structural and design loading study. It focuses on hydraulic and hydrodynamic considerations and reports the study's conclusions.

Copper toxicity to cyanobacteria and its dependence on extracellular ligand concentration and degradation.

Copper toxicity to the cyanobacteriumPlectonema boryanum (UTEX 594) has been investigated in the presence of citrate and nitrilotriacetic acid (NTA) using a copper-saturated culture medium. The coppercitrate complex was biodegradable, and toxicity was dependent on the free ligand concentration. The ratio of citrate to copper also affected the extent of metal toxicity. NTA was not degraded byP. boryanum. Ligand degradation in the cyanobacterial culture increased the ionic copper concentration and caused a concomitant reduction in growth. The ecological implications of these findings are discussed.