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1.
Nat Genet ; 33(4): 527-32, 2003 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-12612585

RESUMEN

Idiopathic generalized epilepsy (IGE) is an inherited neurological disorder affecting about 0.4% of the world's population. Mutations in ten genes causing distinct forms of idiopathic epilepsy have been identified so far, but the genetic basis of many IGE subtypes is still unknown. Here we report a gene associated with the four most common IGE subtypes: childhood and juvenile absence epilepsy (CAE and JAE), juvenile myoclonic epilepsy (JME), and epilepsy with grand mal seizures on awakening (EGMA; ref. 8). We identified three different heterozygous mutations in the chloride-channel gene CLCN2 in three unrelated families with IGE. These mutations result in (i) a premature stop codon (M200fsX231), (ii) an atypical splicing (del74-117) and (iii) a single amino-acid substitution (G715E). All mutations produce functional alterations that provide distinct explanations for their pathogenic phenotypes. M200fsX231 and del74-117 cause a loss of function of ClC-2 channels and are expected to lower the transmembrane chloride gradient essential for GABAergic inhibition. G715E alters voltage-dependent gating, which may cause membrane depolarization and hyperexcitability.


Asunto(s)
Canales de Cloruro/genética , Epilepsia Generalizada/genética , Mutación , Adolescente , Adulto , Secuencia de Bases , Membrana Celular/metabolismo , Codón de Terminación , Análisis Mutacional de ADN , ADN Complementario/metabolismo , Electrofisiología , Salud de la Familia , Femenino , Heterocigoto , Humanos , Masculino , Microscopía Confocal , Microscopía Fluorescente , Modelos Biológicos , Datos de Secuencia Molecular , Linaje , Plásmidos/metabolismo , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa , Transfección
2.
J Neurosci ; 22(17): 7462-70, 2002 Sep 01.
Artículo en Inglés | MEDLINE | ID: mdl-12196568

RESUMEN

Mutations in the muscle chloride channel gene CLCN1 cause myotonia congenita, an inherited disorder of skeletal muscle excitability leading to a delayed relaxation after muscle contraction. Here, we examine the functional consequences of a novel disease-causing mutation that predicts the substitution of alanine by threonine at position 331 (A331T) by whole-cell patch-clamp recording of recombinant mutant channels. A331T hClC-1 channels exhibit a novel slow gate that activates during membrane hyperpolarization and closes at positive potentials. This novel gate acts in series with fast opening and closing transitions that are common to wild-type (WT) and mutant channels. Under conditions at which this novel gate is not activated, i.e., a holding potential of 0 mV, the typical depolarization-induced activation gating of WT hClC-1 was only slightly affected by the mutation. In contrast, A331T hClC-1 channels with an open slow gate display an altered voltage dependence of open probability. These novel gating features of mutant channels produce a decreased open probability at -85 mV, the normal muscle resting potential, leading to a reduced resting chloride conductance of affected muscle fibers. The A331T mutation causes an unprecedented alteration of ClC-1 gating and reveals novel processes defining transitions between open and closed states in ClC chloride channels.


Asunto(s)
Canales de Cloruro/genética , Canales de Cloruro/metabolismo , Activación del Canal Iónico , Miotonía Congénita/genética , Miotonía Congénita/fisiopatología , Adulto , Secuencia de Aminoácidos , Sustitución de Aminoácidos , Línea Celular , Secuencia Conservada , Dimerización , Expresión Génica , Heterocigoto , Humanos , Activación del Canal Iónico/fisiología , Riñón/citología , Riñón/metabolismo , Masculino , Potenciales de la Membrana , Datos de Secuencia Molecular , Mutación , Técnicas de Placa-Clamp , Transfección
3.
Biophys J ; 84(4): 2306-18, 2003 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-12668439

RESUMEN

ClC-4 and ClC-5 are mammalian ClC isoforms with unique ion conduction and gating properties. Macroscopic current recordings in heterologous expression systems revealed very small currents at negative potentials, whereas a substantially larger instantaneous current amplitude and a subsequent activation were observed upon depolarization. Neither the functional basis nor the physiological impact of these channel features are currently understood. Here, we used whole-cell recordings to study pore properties of human ClC-4 channels heterologously expressed in tsA201 or HEK293 cells. Variance analysis demonstrated that the prominent rectification of the instantaneous macroscopic current amplitude is due to a voltage-dependent unitary current conductance. The single channel amplitudes are very small, i.e., 0.10 +/- 0.02 pA at +140 mV for external Cl(-) and internal I(-). Conductivity and permeability sequences were determined for various external and internal anions, and both values increase for anions with lower dehydration energies. ClC-4 exhibits pore properties that are distinct from other ClC isoforms. These differences can be explained by assuming differences in the size of the pore narrowing and the electrostatic potentials within the ion conduction pathways.


Asunto(s)
Permeabilidad de la Membrana Celular/fisiología , Canales de Cloruro/clasificación , Canales de Cloruro/fisiología , Cloro/fisiología , Riñón/embriología , Potenciales de la Membrana/fisiología , Aniones/farmacocinética , Línea Celular , Conductividad Eléctrica , Humanos , Activación del Canal Iónico/fisiología , Riñón/fisiología , Porosidad , Proteínas Recombinantes de Fusión/clasificación , Proteínas Recombinantes de Fusión/fisiología , Especificidad de la Especie
4.
Brain ; 125(Pt 11): 2392-407, 2002 Nov.
Artículo en Inglés | MEDLINE | ID: mdl-12390967

RESUMEN

Myotonia is a condition characterized by impaired relaxation of muscle following sudden forceful contraction. We systematically screened all 23 exons of the CLCN1 gene in 88 unrelated patients with myotonia and identified mutations in 14 patients. Six novel mutations were discovered: five were missense (S132C, L283F, T310M, F428S and T550M) found in heterozygous patients, and one was a nonsense mutation (E193X) in a homozygous patient. While five patients had a clinical diagnosis of myotonia congenita, the patient with the F428S mutation exhibited symptoms characteristic of paramyotonia congenita--a condition usually thought to be caused by mutations in the sodium channel gene SCN4A. Nevertheless, no mutations in SCN4A were identified in this patient. The functional consequences of the novel CLCN1 sequence variants were explored by recording chloride currents from human embryonic kidney cells transiently expressing homo- or heterodimeric mutant channels. The five tested mutations caused distinct functional alterations of the homodimeric human muscle chloride ion channel hClC-1. S132C and T550M conferred novel hyperpolarization-induced gating steps, L283F and T310M caused a shift of the activation curve to more positive potentials and F428S reduced the expression level of hClC-1 channels. All showed a dominant-negative effect. For S132C, L283F, T310M and T550M, heterodimeric channels consisting of one wild-type (WT) and one mutant subunit exhibited a shifted activation curve at low intracellular [Cl(-)]. WT-F428S channels displayed properties similar to WT hClC-1, but expressed at significantly lower levels. The novel mutations exhibit a broad variety of functional defects that, by distinct mechanisms, cause a significant reduction of the resting chloride conductance in muscle of heterozygous patients. Our results provide novel insights into functional alterations and clinical symptoms caused by mutations in CLCN1.


Asunto(s)
Canales de Cloruro/deficiencia , Músculo Esquelético/metabolismo , Mutación/genética , Miotonía Congénita/genética , Adulto , Empalme Alternativo/genética , Secuencia de Aminoácidos/genética , Membrana Celular/genética , Membrana Celular/metabolismo , Canales de Cloruro/genética , Análisis Mutacional de ADN , Femenino , Genotipo , Humanos , Masculino , Persona de Mediana Edad , Músculo Esquelético/patología , Músculo Esquelético/fisiopatología , Mutación Missense/genética , Miotonía Congénita/metabolismo , Miotonía Congénita/fisiopatología
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