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1.
Proc Natl Acad Sci U S A ; 120(28): e2218900120, 2023 07 11.
Artículo en Inglés | MEDLINE | ID: mdl-37399384

RESUMEN

Milk production is an ancient adaptation that unites all mammals. Milk contains a microbiome that can contribute to offspring health and microbial-immunological development. We generated a comprehensive milk microbiome dataset (16S rRNA gene) for the class Mammalia, representing 47 species from all placental superorders, to determine processes structuring milk microbiomes. We show that across Mammalia, milk exposes offspring to maternal bacterial and archaeal symbionts throughout lactation. Deterministic processes of environmental selection accounted for 20% of milk microbiome assembly processes; milk microbiomes were similar from mammals with the same host superorder (Afrotheria, Laurasiathera, Euarchontoglires, and Xenarthra: 6%), environment (marine captive, marine wild, terrestrial captive, and terrestrial wild: 6%), diet (carnivore, omnivore, herbivore, and insectivore: 5%), and milk nutrient content (sugar, fat, and protein: 3%). We found that diet directly and indirectly impacted milk microbiomes, with indirect effects being mediated by milk sugar content. Stochastic processes, such as ecological drift, accounted for 80% of milk microbiome assembly processes, which was high compared to mammalian gut and mammalian skin microbiomes (69% and 45%, respectively). Even amid high stochasticity and indirect effects, our results of direct dietary effects on milk microbiomes provide support for enteromammary trafficking, representing a mechanism by which bacteria are transferred from the mother's gut to mammary gland and then to offspring postnatally. The microbial species present in milk reflect both selective pressures and stochastic processes at the host level, exemplifying various ecological and evolutionary factors acting on milk microbiomes, which, in turn, set the stage for offspring health and development.


Asunto(s)
Microbioma Gastrointestinal , Microbiota , Animales , Femenino , Embarazo , Leche , Microbioma Gastrointestinal/genética , ARN Ribosómico 16S/genética , Placenta , Microbiota/genética , Mamíferos/genética , Bacterias/genética , Euterios/genética
2.
Environ Microbiol ; 26(6): e16664, 2024 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-38830671

RESUMEN

Milk is a complex biochemical fluid that includes macronutrients and microbiota, which, together, are known to facilitate infant growth, mediate the colonization of infant microbiomes, and promote immune development. Examining factors that shape milk microbiomes and milk-nutrient interplay across host taxa is critical to resolving the evolution of the milk environment. Using a comparative approach across four cercopithecine primate species housed at three facilities under similar management conditions, we test for the respective influences of the local environment (housing facility) and host species on milk (a) macronutrients (fat, sugar, and protein), (b) microbiomes (16S rRNA), and (c) predicted microbial functions. We found that milk macronutrients were structured according to host species, while milk microbiomes and predicted function were strongly shaped by the local environment and, to a lesser extent, host species. The milk microbiomes of rhesus macaques (Macaca mulatta) at two different facilities more closely resembled those of heterospecific facility-mates compared to conspecifics at a different facility. We found similar, facility-driven patterns of microbial functions linked to physiology and immune modulation, suggesting that milk microbiomes may influence infant health and development. These results provide novel insight into the complexity of milk and its potential impact on infants across species and environments.


Asunto(s)
Microbiota , Leche , Nutrientes , ARN Ribosómico 16S , Animales , Leche/microbiología , Nutrientes/metabolismo , ARN Ribosómico 16S/genética , Macaca mulatta/microbiología , Femenino , Cercopithecidae/microbiología , Bacterias/clasificación , Bacterias/genética , Bacterias/aislamiento & purificación , Evolución Biológica
3.
Glob Chang Biol ; 30(2): e17193, 2024 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-38380447

RESUMEN

The Arctic is the fastest warming biome on the planet, and environmental changes are having striking effects on freshwater ecosystems that may impact the regional carbon cycle. The metabolic state of Arctic lakes is often considered net heterotrophic, due to an assumed supply of allochthonous organic matter that supports ecosystem respiration and carbon mineralization in excess of rates of primary production. However, lake metabolic patterns vary according to regional climatic characteristics, hydrological connectivity, organic matter sources and intrinsic lake properties, and the metabolism of most Arctic lakes is unknown. We sampled 35 waterbodies along a connectivity gradient from headwater to downstream lakes, on southern Victoria Island, Nunavut, in an area characterized by low precipitation, organic-poor soils, and high evaporation rates. We evaluated whether lakes were net autotrophic or heterotrophic during the open water period using an oxygen isotopic mass balance approach. Most of the waterbodies were autotrophic and sites of net organic matter production or close to metabolic equilibrium. Autotrophy was associated with higher benthic primary production, as compared to its pelagic counterpart, due to the high irradiance reaching the bottom and efficient internal carbon and nutrient cycling. Highly connected midstream and downstream lakes showed efficient organic matter cycling, as evidenced by the strong coupling between gross primary production (GPP) and ecosystem respiration, while decoupling was observed in some headwater lakes with significantly higher GPP. The shallow nature of lakes in the flat, arid region of southern Victoria Island supports net autotrophy in most lakes during the open water season. Ongoing climate changes that lengthen the ice-free irradiance period and increase rates of nutrient evapoconcentration may further promote net autotrophy, with uncertain long-term effects for lake functioning.


Asunto(s)
Ecosistema , Lagos , Nunavut , Canadá , Procesos Autotróficos , Ciclo del Carbono , Carbono/metabolismo , Agua
4.
Am J Primatol ; 86(1): e23570, 2024 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-37876290

RESUMEN

Milk composition is a fundamental aspect of mammalian reproduction. Differences in milk composition between species may reflect phylogeny, dietary ecology, lactation strategy, and infant growth patterns, but may also vary within a species due to maternal body condition. This study presents the first published data on milk macronutrient composition of southern pig-tailed macaques (Macaca nemestrina) and compares the results with data on two other Cercopithecine species. Milk samples were obtained from five dams at 10- and 14-weeks postparturition. Macronutrient composition was determined at the Smithsonian's National Zoo and Conservation Biology Institute using proven methods developed over 30 years. On average (±SEM), the milk contained 83.9 ± 0.4% water, 6.7 ± 0.4% fat, 7.6 ± 0.1% sugar, 1.8 ± 0.1% protein, and 0.22 ± 0.01% mineral content. The Ca:P ratio was 1.8; concentrations of Ca and protein were correlated. Mean gross energy was 1.02 ± 0.03 kcal/g with most of the energy coming from fat (59.6 ± 1.5%), followed by sugar (29.9 ± 1.4%) and protein (10.5 ± 0.5%). The milks at 14 weeks of infant age were higher in energy than the milks at 10 weeks, with an increase in energy from fat (p = 0.005) and decrease in energy from sugar (p = 0.018). The energy from protein did not change (p = 0.272). Compared to captive rhesus macaque (Macaca mulatta) and olive baboon (Papio anubis) milk assayed by identical methods, captive pig-tailed macaque milk was higher in energy, but after accounting for the higher milk energy there was no difference in the proportions of milk energy from protein, fat, and sugar. The captive pig-tailed dams were significantly heavier than reported values for wild pig-tailed macaques, suggesting high body condition. High body condition in captive Cercopithecines appears to result in milk higher in energy, with more energy coming from fat and less from sugar. However, variation in the proportion of milk energy from protein in captive Cercopithecine milks appears relatively constrained.


Asunto(s)
Leche , Nutrientes , Animales , Femenino , Macaca mulatta , Macaca nemestrina , Leche/química , Azúcares
5.
Artículo en Inglés | MEDLINE | ID: mdl-38253199

RESUMEN

Fish gills are complex organs that have direct contact with the environment and perform numerous functions including gas exchange and ion regulation. Determining if gill morphometry can change under different environmental conditions to maintain and/or improve gas exchange and ion regulation is important for understanding if gill plasticity can improve survival with increasing environmental change. We assessed gill morphology (gas exchange and ion regulation metrics), hematocrit and gill Na+/K+ ATPase activity of wild-captured blackside darter (Percina maculata), greenside darter (Etheostoma blennioides), and johnny darter (Etheostoma nigrum) at two temperatures (10 and 25 °C) and turbidity levels (8 and 94 NTU). Samples were collected August and October 2020 in the Grand River to assess temperature differences, and August 2020 in the Thames River to assess turbidity differences. Significant effects of temperature and/or turbidity only impacted ionocyte number, lamellae width, and hematocrit. An increase in temperature decreased ionocyte number while an increase in turbidity increased lamellae width. Hematocrit had a species-specific response for both temperature and turbidity. Findings suggest that the three darter species have limited plasticity in gill morphology, with no observed compensatory changes in hematocrit or Na+/K+ ATPase activity to maintain homeostasis under the different environmental conditions.


Asunto(s)
Branquias , Ríos , Animales , Temperatura , Branquias/metabolismo , Sodio/metabolismo , Adenosina Trifosfatasas , ATPasa Intercambiadora de Sodio-Potasio/metabolismo
6.
Telemed J E Health ; 30(1): 198-203, 2024 01.
Artículo en Inglés | MEDLINE | ID: mdl-37466478

RESUMEN

Background: Maternal-fetal medicine (MFM) is a medical subspecialty that cares for patients with high-risk pregnancies. Methods: An IRB-approved survey was offered to patients in MFM offices of a tertiary health care system from March 2022 to May 2022. Demographics and responses to 15 statements about telemedicine were collected via a 5-point Likert scale. Exploratory factor analysis was performed to identify factors affecting patient perception of the telemedicine experience. We sought to examine perception of telemedicine in MFM, identify factors affecting perception, and identify whether specific demographics were associated with patients who view telemedicine appointments favorably. Results: Surveys were completed by 327 of 347 (94%) patients. A total of 233 (71%) patients felt that the telemedicine experience was equal in quality to in-person appointments, and 257 (79%) were open to telemedicine appointments in the future. Exploratory factor analysis yielded two factors: "physician attentiveness" and "technology comfort." Telemedicine was viewed favorably or neutrally for both factors. Education (lower) and marital status (single) were associated with a favorable perception of physician attentiveness. Ethnicity (Hispanic), employment status (employed), and prior telemedicine experience were associated with a favorable perception of technology comfort. Conclusion: Most patients felt the quality of telemedicine appointments was equal to those completed in person. Physician attentiveness and technology comfort affect telemedicine perception. Specific patient demographic characteristics were associated with differing perceptions of telemedicine in MFM. Our findings suggest that telemedicine is positively viewed for MFM and can be used for improving health care delivery efficiency in MFM.


Asunto(s)
Obstetricia , Telemedicina , Embarazo , Femenino , Humanos , Perinatología , Atención a la Salud , Percepción
7.
J Fish Biol ; 104(3): 797-806, 2024 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-37986023

RESUMEN

The recent, rapid spreading of non-native pink salmon Oncorhynchus gorbuscha in the North Atlantic area has raised concerns about their possible negative impacts on native salmonid species. Potential interactions include competition for food resources during the short freshwater phase of juvenile O. gorbuscha, but little is known about their feeding behavior in the newly occupied North Atlantic rivers. Using stable isotope and stomach content analyses, patterns of freshwater feeding of non-native O. gorbuscha fry were studied in a large Fennoscandian river, the Teno, that discharges to the Barents Sea. Changes in stable isotope values (δ13 C, δ15 N, δ34 S) and stomach contents from the period of emergence (April to mid-May) to estuarine entry (late May/June) were examined and provided both temporally integrated and short-term indicators of freshwater feeding dependency. In addition, the occurrence of juvenile O. gorbuscha and changes in their length and weight during their emergence/migration period were investigated. Juvenile O. gorbuscha were at the spawning grounds from April through to mid-May with abundance peaking in mid-May. Fish moved to the estuary by late May and their abundance decreased toward June, and their body size increased concurrently. Stomach analyses indicated no feeding activity in April-early May in the spawning areas, but the stomach fullness indices increased markedly in fish sampled in the estuary in May and June. The most important prey items in stomachs were Chironomidae and Ephemeroptera larvae. Significant changes in all analysed stable isotopes were detected among sample periods, with a peak in mid-May and June showing significantly lower values than other sample periods. A change from the higher values reflective of parental marine feeding to the lower values reflective of freshwater feeding indicated active in-river feeding by juveniles during the study period. The documented active freshwater feeding of non-native juvenile O. gorbuscha suggests potential resource competition with native fluvial fishes, particularly salmonids.


Asunto(s)
Ríos , Salmón , Animales , Agua Dulce , Dieta/veterinaria , Isótopos
8.
J Fish Biol ; 104(6): 1888-1898, 2024 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-38506425

RESUMEN

Anthropogenic stressors such as agriculture and urbanization can increase river turbidity, which can negatively impact fish gill morphology and growth due to reduced oxygen in the benthic environment. We assessed the gill morphology, field metabolic rate (FMR), and two hypoxia tolerance metrics (oxygen partial pressure at loss of equilibrium, PO2 at LOE, and critical oxygen tension, Pcrit) of eastern sand darter (Ammocrypta pellucida), a small benthic fish listed as threatened under the Species at Risk Act in Canada, from rivers in southern Ontario. Field trials were conducted streamside in the Grand River (August 2019; mean NTU 8) and in the comparatively more turbid Thames River (August 2020; mean NTU 94) to test the effect of turbidity on each physiological endpoint. Gills were collected from incidental mortalities and museum specimens, and were assessed using hematoxylin and eosin and immunofluorescent staining. The between-river comparison indicated that turbidity significantly increased interlamellar space and filament width but had no significant influence on other gill morphometrics or FMR. Turbidity significantly increased PO2 at LOE (i.e., fish had a lower hypoxia tolerance) but did not significantly impact Pcrit. Therefore, although turbidity influences hypoxia tolerance through LOE, turbidity levels were not sufficiently high in the study rivers to contribute to measurable changes in gill morphology or metabolism in the wild. Determining whether changes in gill morphology or metabolism occur under higherturbidity levels would help resolve the ecological importance of turbidity on species physiology in urban and agricultural ecosystems.


Asunto(s)
Branquias , Oxígeno , Ríos , Animales , Branquias/anatomía & histología , Branquias/fisiología , Ontario , Oxígeno/metabolismo , Hipoxia , Perciformes/fisiología , Perciformes/anatomía & histología
9.
Proc Biol Sci ; 290(1996): 20230262, 2023 04 12.
Artículo en Inglés | MEDLINE | ID: mdl-37040803

RESUMEN

Understanding the factors shaping patterns of ecological resilience is critical for mitigating the loss of global biodiversity. Throughout aquatic environments, highly mobile predators are thought to serve as important vectors of energy between ecosystems thereby promoting stability and resilience. However, the role these predators play in connecting food webs and promoting energy flow remains poorly understood in most contexts. Using carbon and nitrogen isotopes, we quantified the use of several prey resource pools (small oceanic forage, large oceanics, coral reef, and seagrass) by 17 species of elasmobranch fishes (n = 351 individuals) in The Bahamas to determine their functional diversity and roles as ecosystem links. We observed remarkable functional diversity across species and identified four major groups responsible for connecting discrete regions of the seascape. Elasmobranchs were responsible for promoting energetic connectivity between neritic, oceanic and deep-sea ecosystems. Our findings illustrate how mobile predators promote ecosystem connectivity, underscoring their functional significance and role in supporting ecological resilience. More broadly, strong predator conservation efforts in developing island nations, such as The Bahamas, are likely to yield ecological benefits that enhance the resilience of marine ecosystems to combat imminent threats such as habitat degradation and climate change.


Asunto(s)
Ecosistema , Elasmobranquios , Animales , Arrecifes de Coral , Biodiversidad , Peces
10.
J Anim Ecol ; 92(12): 2333-2347, 2023 12.
Artículo en Inglés | MEDLINE | ID: mdl-37843043

RESUMEN

Foraging is a behavioural process and, therefore, individual behaviour and diet are theorized to covary. However, few comparisons of individual behaviour type and diet exist in the wild. We tested whether behaviour type and diet covary in a protected population of Atlantic cod, Gadus morhua. Working in a no-take marine reserve, we could collect data on natural behavioural variation and diet choice with minimal anthropogenic disturbance. We inferred behaviour using acoustic telemetry and diet from stable isotope compositions (expressed as δ13 C and δ15 N values). We further investigated whether behaviour and diet could have survival costs. We found cod with shorter diel vertical migration distances fed at higher trophic levels. Cod δ13 C and δ15 N values scaled positively with body size. Neither behaviour nor diet predicted survival, indicating phenotypic diversity is maintained without survival costs for cod in a protected ecosystem. The links between diet and diel vertical migration highlight that future work is needed to understand whether the shifts in this behaviour during environmental change (e.g. fishing or climate), could lead to trophic cascades.


Asunto(s)
Ecosistema , Gadus morhua , Animales , Clima , Isótopos , Conducta Espacial
11.
Oecologia ; 202(2): 353-368, 2023 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-37291257

RESUMEN

Research from terrestrial communities shows that diminished predation risk is a principal driver of heterospecific grouping behavior, with foraging ecology predicting the roles that species play in groups, as more vulnerable foragers preferentially join more vigilant ones from whom they can benefit. Meanwhile, field studies examining the adaptive significance of heterospecific shoaling among marine fish have focused disproportionately on feeding advantages such as scrounging or prey-flushing. Juvenile bonefish (Albula vulpes) occur almost exclusively among mojarras (Eucinostomus spp.) and even elect to join them over conspecifics, suggesting they benefit from doing so. We evaluated the roles of risk-related and food-related factors in motivating this pattern of affiliation, estimating: (1) the relative levels of risk associated with each species' search and prey capture activities, via behavioral vulnerability traits discerned from in situ video of heterospecific shoals, and (2) resource use redundancy, using stable isotopes (δ13C, δ15N, and δ34S) to quantify niche overlap. Across four distinct metrics, bonefish behaviors implied a markedly greater level of risk than those of mojarras, typified by higher activity levels and a reduced capacity for overt vigilance; consistent with expectations if their association conformed to patterns of joining observed in terrestrial habitats. Resource use overlap inferred from stable isotopes was low, indicating that the two species partitioned resources and making it unlikely that bonefish derived substantive food-related benefits. Collectively, these findings suggest that the attraction of juvenile bonefish to mojarras is motivated primarily by antipredator advantages, which may include the exploitation of risk-related social cues.


Asunto(s)
Ecosistema , Peces , Animales , Conducta Predatoria , Isótopos , Ecología
12.
J Fish Biol ; 103(5): 884-896, 2023 Nov.
Artículo en Inglés | MEDLINE | ID: mdl-37349978

RESUMEN

Alternative migratory tactics in salmonids reflect the large observed interindividual variation in spatial behaviour which may range from strict freshwater residency to uninterrupted anadromy. In Salvelinus, sea migrations are performed during the ice-free period as freshwater overwintering is thought to be obligatory due to physiological constraints. As a result, individuals can either migrate the next spring or remain in freshwater, as anadromy is generally considered facultative. In Arctic charr (Salvelinus alpinus), skipped migrations are known to occur, but limited data are available regarding their frequencies within and among populations. Here, the authors used an otolith microchemistry approach relying on strontium (88 Sr) to infer movements between freshwater and marine habitats, and annual oscillations in zinc (64 Zn) to help with age identification. They determined the age-at-first-migration and the occurrence of subsequent annual migrations in two Nunavik Arctic charr populations sampled in Deception Bay (Salluit) and river systems linked to Hopes Advance Bay (Aupaluk), northern Québec, Canada. The mode for age-at-first-migration was 4+ for both populations, although it exhibited large variation (range: 0+ to 8+). Skipped migrations constituted a rare event, as 97.7% and 95.6% of the examined Arctic charr at Salluit (n = 43, mean age = 10.3 ± 2.0 years) and Aupaluk (n = 45, mean age = 6.0 ± 1.9 years), respectively, were found to have performed uninterrupted annual migrations after initiation of the behaviour. The consistency of the annual migrations suggests that the tactic is sufficiently fitness rewarding to be maintained under current environmental conditions. From a fisheries management perspective, these repeated migrations combined with low site fidelity in this species may lead to large interannual variations in abundance at the local scale, which may represent a challenge for monitoring Arctic charr demographics on a river-by-river basis.


Asunto(s)
Agua Dulce , Membrana Otolítica , Humanos , Animales , Canadá , Quebec , Trucha/fisiología
13.
J Proteome Res ; 21(11): 2647-2654, 2022 11 04.
Artículo en Inglés | MEDLINE | ID: mdl-36278968

RESUMEN

Milk is a critical nutrition source for all neonatal mammals. In addition to nutrition, milk contains a multitude of bioactive molecules that likely affect neonatal physiology, metabolism, and immune function. We suggest that changes in the milk proteome across lactation reflect the changing need of the neonate and juvenile offspring. We used mass spectrometry to characterize the milk proteomes from a Pongo pygmaeus (12 samples) and a Gorilla gorilla (6 samples) housed at the Smithsonian's National Zoo and Conservation Biology Institute and trained to give milk samples. We found a total of 454 proteins from P. pygmaeus and 428 proteins from G. gorilla. We specifically characterized changes across lactation in 13 proteins representing multiple compartments of milk, including the milk fat globule membrane and whey. Additionally, we characterized changes in various immunoglobulin types, finding similarities to previously published studies on primate milks. Despite broad similarities between the milk proteomes of these two apes, we demonstrated that proteomes from samples from 8 to 12 months clustered by species/individual and were distinct. Samples from more individuals are required to distinguish whether our result demonstrates species differences or individual differences. This study represents a baseline study that other zoo-based milk studies can build from. All RAW data, MetaMorpheus search results, and PAW_BLAST results are available on MassIVE at ftp://massive.ucsd.edu/MSV000089723/.


Asunto(s)
Gorilla gorilla , Pongo pygmaeus , Animales , Femenino , Gorilla gorilla/fisiología , Proteínas de la Leche , Proteoma , Lactancia/fisiología , Primates , Pan troglodytes , Mamíferos
14.
J Fish Biol ; 100(2): 416-424, 2022 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-34786715

RESUMEN

Reproductive phenology and the length of the growing season vary in response to interannual environmental variability, with implications for population dynamics of freshwater fishes. Understanding the reproductive phenology of imperilled species in relation to environmental conditions is needed to better evaluate potential responses to changing environmental conditions, estimate future population dynamics and develop comprehensive recovery strategies. We examined Silver Shiner, a species listed as "Threatened" under Canada's Species at Risk Act, during spring 2018 and 2019 to better understand the reproductive phenology of the species at the northern edge of its range in Canada. The initiation of Silver Shiner spawning occurred on the descending limb of the hydrograph and was completed before the onset of the extended period of low summer flow. In addition, both the initiation and cessation of spawning occurred in response to a cumulative growing degree day base 5 (GDD5 ) cue, with logistic regression models indicating a 50% probability the population initiated and ceased spawning when GDD5 reached 68°C•days and 368°C•days, respectively. Logistic regression incorporating GDD5 effectively predicted spawning initiation and cessation, providing useful models for examining the impacts of alterations to the thermal regime on reproductive phenology and improving the ability to evaluate changes in the larval growth period. Furthermore, the models can facilitate the development of real-time estimates of spawning activity, and therefore ensure that disturbance to the species is minimized during the sensitive reproductive period.


Asunto(s)
Señales (Psicología) , Cyprinidae , Animales , Reproducción , Estaciones del Año
15.
Zoo Biol ; 41(4): 360-364, 2022 Jul.
Artículo en Inglés | MEDLINE | ID: mdl-35049088

RESUMEN

In January 2017, a Nile hippopotamus (Hippopotamus amphibious) was born approximately six weeks premature at the Cincinnati Zoo & Botanical Garden. Due to the calf's weakened condition and lack of interest from the dam, management at the zoo made the decision to hand-rear the calf. Limited published information was available on hand-rearing this species of hippopotamus (hippo). To devise a nutritionally appropriate recipe, milk samples were acquired from the dam on Days 1, 3, 8, and 9 postpartum, and assayed for sugar, protein, fat, mineral, and water content using standard methods validated for multiple species of mammals at the Smithsonian National Zoo's Nutrition Science Laboratory. The sugar content stayed relatively constant (mean = 4.5%; range: 4.3%-4.7%). The fat consistently increased from 0.48% to 4.24% (mean = 2%). Excluding Day 9, the protein content gradually decreased from 9.56% to 6.39% (mean = 8%). The dry matter (DM) ranged from 14.38% to 16.72% (i.e., water content of 85.62%-83.28%), with the sum of the solids (sugar, protein, fat, and ash) averaging 98.5% of measured DM. Fat content was lower than expected but within the range of other artiodactyls. Between Days 1 and 8, the trend of decreasing protein and increasing fat was consistent with a change from colostrum to mature milk. The sharp increase in fat and protein with a decrease in sugar on Day 9 may indicate the beginning of the involution of the mammary gland due to lack of nursing stimulus. Utilizing this information, the Cincinnati Zoo was able to formulate a successful milk replacement recipe that allowed the calf to be raised through weaning to maturity.


Asunto(s)
Artiodáctilos , Leche , Animales , Animales de Zoológico , Carbohidratos , Femenino , Proteínas , Azúcares , Agua
16.
J Med Primatol ; 50(3): 164-175, 2021 06.
Artículo en Inglés | MEDLINE | ID: mdl-33913156

RESUMEN

BACKGROUND: A survey was developed to characterize disease incidence, common pathology lesions, environmental characteristics, and nutrition programs within captive research marmoset colonies. METHODS: Seventeen research facilities completed the electronic survey. RESULTS: Nutritional management programs varied amongst research institutions housing marmosets; eight primary base diets were reported. The most common clinical syndromes reported were gastrointestinal disease (i.e. inflammatory bowel disease like disease, chronic lymphocytic enteritis, chronic malabsorption, chronic diarrhea), metabolic bone disease or fracture, infectious diarrhea, and oral disease (tooth root abscesses, gingivitis, tooth root resorption). The five most common pathology morphologic diagnoses were colitis, nephropathy/nephritis, enteritis, chronic lymphoplasmacytic enteritis, and cholecystitis. Obesity was more common (average 20% of a reporting institution's population) than thin body condition (average 5%). CONCLUSIONS: Through review of current practices, we aim to inspire development of evidence-based practices to standardize husbandry and nutrition practices for marmoset research colonies.


Asunto(s)
Enfermedades Óseas Metabólicas , Callithrix , Animales , Dieta/veterinaria , Incidencia , Obesidad
17.
Am J Respir Crit Care Med ; 202(6): 812-821, 2020 09 15.
Artículo en Inglés | MEDLINE | ID: mdl-32584597

RESUMEN

Rationale: Coronavirus disease (COVID-19) is a global threat to health. Its inflammatory characteristics are incompletely understood.Objectives: To define the cytokine profile of COVID-19 and to identify evidence of immunometabolic alterations in those with severe illness.Methods: Levels of IL-1ß, IL-6, IL-8, IL-10, and sTNFR1 (soluble tumor necrosis factor receptor 1) were assessed in plasma from healthy volunteers, hospitalized but stable patients with COVID-19 (COVIDstable patients), patients with COVID-19 requiring ICU admission (COVIDICU patients), and patients with severe community-acquired pneumonia requiring ICU support (CAPICU patients). Immunometabolic markers were measured in circulating neutrophils from patients with severe COVID-19. The acute phase response of AAT (alpha-1 antitrypsin) to COVID-19 was also evaluated.Measurements and Main Results: IL-1ß, IL-6, IL-8, and sTNFR1 were all increased in patients with COVID-19. COVIDICU patients could be clearly differentiated from COVIDstable patients, and demonstrated higher levels of IL-1ß, IL-6, and sTNFR1 but lower IL-10 than CAPICU patients. COVID-19 neutrophils displayed altered immunometabolism, with increased cytosolic PKM2 (pyruvate kinase M2), phosphorylated PKM2, HIF-1α (hypoxia-inducible factor-1α), and lactate. The production and sialylation of AAT increased in COVID-19, but this antiinflammatory response was overwhelmed in severe illness, with the IL-6:AAT ratio markedly higher in patients requiring ICU admission (P < 0.0001). In critically unwell patients with COVID-19, increases in IL-6:AAT predicted prolonged ICU stay and mortality, whereas improvement in IL-6:AAT was associated with clinical resolution (P < 0.0001).Conclusions: The COVID-19 cytokinemia is distinct from that of other types of pneumonia, leading to organ failure and ICU need. Neutrophils undergo immunometabolic reprogramming in severe COVID-19 illness. Cytokine ratios may predict outcomes in this population.


Asunto(s)
Reacción de Fase Aguda/inmunología , Proteínas Portadoras/metabolismo , Infecciones por Coronavirus/inmunología , Infecciones por Coronavirus/metabolismo , Citocinas/inmunología , Subunidad alfa del Factor 1 Inducible por Hipoxia/metabolismo , Ácido Láctico/metabolismo , Proteínas de la Membrana/metabolismo , Neumonía Viral/inmunología , Neumonía Viral/metabolismo , Hormonas Tiroideas/metabolismo , alfa 1-Antitripsina/inmunología , Reacción de Fase Aguda/metabolismo , Adulto , Anciano , Betacoronavirus , Western Blotting , COVID-19 , Estudios de Casos y Controles , Infecciones Comunitarias Adquiridas/inmunología , Infecciones Comunitarias Adquiridas/metabolismo , Infecciones por Coronavirus/mortalidad , Infecciones por Coronavirus/fisiopatología , Enfermedad Crítica , Electroforesis en Gel de Poliacrilamida , Ensayo de Inmunoadsorción Enzimática , Femenino , Hospitalización , Humanos , Unidades de Cuidados Intensivos , Interleucina-10/inmunología , Interleucina-1beta/inmunología , Interleucina-6/inmunología , Interleucina-8/inmunología , Tiempo de Internación , Masculino , Persona de Mediana Edad , Neutrófilos/inmunología , Neutrófilos/metabolismo , Pandemias , Fosforilación , Neumonía/inmunología , Neumonía/metabolismo , Neumonía Viral/mortalidad , Neumonía Viral/fisiopatología , Receptores Tipo I de Factores de Necrosis Tumoral/inmunología , SARS-CoV-2 , Índice de Severidad de la Enfermedad , alfa 1-Antitripsina/metabolismo , Proteínas de Unión a Hormona Tiroide
18.
Am J Primatol ; 83(9): e23315, 2021 09.
Artículo en Inglés | MEDLINE | ID: mdl-34339526

RESUMEN

This study was designed to (1) characterize the macronutrient composition of olive baboon (Papio anubis) milk, (2) compare baboon milk composition to that of rhesus macaques (Macaca mulatta), and (3) evaluate the association between the proportion of milk energy derived from protein and relative growth rate within anthropoid primates. A single milk sample was collected from each of eight lactating olive baboons ranging between 47- and 129-days postparturition and six rhesus macaques from 15- to 92-days living at the same institution under identical management conditions. Macronutrient composition (water, fat, protein sugar, and ash) was determined using standard techniques developed at the Nutrition Laboratory at the Smithsonian National Zoological Park. Baboon milk on average contained 86.0% ± 0.6% water, 4.7% ± 0.5% fat, 1.6% ± 0.04% protein, 7.3% ± 0.07% sugar, and 0.165% ± 0.007% ash. Baboon milk gross energy (GE) averaged 0.81 ± 0.04 kcal/g with 51.9% ± 2.6% from fat, 11.8% ± 0.7% from protein, and 36.2% ± 2.0% from sugar. Baboon milk demonstrated strong similarity to milk composition of the closely phylogenetically related rhesus macaque (86.1% ± 0.3% water, 4.1% ± 0.4% fat, 1.69% ± 0.05% protein, 7.71% ± 0.08% sugar, 0.19% ± 0.01% ash, and 0.78 kcal/g). There was no statistical difference between baboon and macaque milk in the proportions of energy from fat, sugar, and protein. Baboon milk can be described as a high sugar, moderate fat, and low protein milk with moderate energy density, which is consistent with their lactation strategy characterized by frequent, on-demand nursing and relatively slow life history compared to nonprimate mammal taxa. The milk energy from protein of both baboon and macaque (12.8% ± 0.3%) milk was intermediate between the protein milk energy of platyrrhine (19.3%-23.2%) and hominoid (8.9%-12.6%) primates, consistent with their relative growth rates also being intermediate. Compared to these cercopithecid monkeys, platyrrhine primates have both higher relative growth rates and higher milk energy from protein, while apes tend to be lower in both.


Asunto(s)
Leche , Papio anubis , Animales , Femenino , Lactancia , Macaca mulatta , Nutrientes , Papio
19.
J Fish Biol ; 98(2): 526-536, 2021 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-33085087

RESUMEN

Subsequent to their introduction in the 1950s, Arctic charr Salvelinus alpinus have been able to establish a self-sustaining population that has adapted to the unique conditions of the sub-Antarctic Kerguelen Islands. Here, 48 individuals (198-415 mm) were caught with gillnets and their basic biology and feeding ecology were examined using stable isotope analysis. The Lac des Fougères population split use of littoral and pelagic resources evenly, although larger fish relied more heavily on littoral production and appear to follow the size-dependent life history habitat template seen in many Scandinavian lakes where smaller sized individuals occupy the pelagic zone and larger individuals dominate the littoral habitat. In Kerguelen, Arctic charr mature at the same ages (5.6 years) as Arctic charr in both sub-Arctic and Arctic lakes. Although mortality was average in comparison to comparator sub-Arctic lakes, it was high in comparison to Arctic lakes. Maximal age (>7+) was at the lower end of the range typically seen in sub-Arctic lakes. Although they inhabit a resource-poor environment, Kerguelen Arctic charr showed no evidence of cannibalism. Thus, while Arctic charr can survive and reproduce in the relatively unproductive Kerguelen lake environments, survival and growth nevertheless appear to be traded off against survival and longevity. The uniqueness of the population location and the recency of its introduction suggest that further monitoring of the population has the potential to yield valuable insights into both the adaptability of the species and its likely responses to ongoing large-scale environmental change as represented by climate change.


Asunto(s)
Conducta Alimentaria/fisiología , Reproducción/fisiología , Trucha/fisiología , Animales , Regiones Antárticas , Tamaño Corporal , Cambio Climático , Ecosistema , Islas , Lagos
20.
J Fish Biol ; 99(3): 1110-1124, 2021 Sep.
Artículo en Inglés | MEDLINE | ID: mdl-34060093

RESUMEN

Lipids, particularly fatty acids (FAs), are major sources of energy and nutrients in aquatic ecosystems and play key roles during vertebrate development. The European eel Anguilla anguilla goes through major biochemical and physiological changes throughout its lifecycle as it inhabits sea- (SW), and/or brackish- (BW) and/or freshwater (FW) habitats. With the ultimate goal being to understand the reasons for eels adopting a certain life history strategy (FW or SW residency vs. 'habitat shifting'), we explored differences in lipid content and FA composition of muscle, liver and eyes from eels collected across Norwegian SW, BW and FW habitats, and at different lifecycle stages (yellow to silver). FW and SW eels had a higher lipid content overall compared to BW eels, reflecting differences in food availability and life history strategies. SW eels had higher proportions of certain monounsaturated FAs (MUFAs; 18:1n-9, 20:1n-9), and of the essential polyunsaturated FAs 20:5n-3 (eicosapentaenoic acid, EPA) and 22:6n-3 (docosahexaenoic acid) than FW eels, reflecting a marine-based diet. In contrast, the muscle of FW eels had higher proportions of 18:3n-3, 18:2n-6 and 20:4n-6 (arachidonic acid), as is typical of FW organisms. MUFA proportions increased in later stage eels, consistent with the hypothesis that the eels accumulate energy stores prior to migration. In addition, the decrease of EPA with advancing stage may be associated with the critical role that this FA plays in eel sexual development. Lipid and FA information provided further understanding of the habitat use and overall ecology of this critically endangered species.


Asunto(s)
Anguilla , Ecosistema , Anguilla/metabolismo , Anguilla/fisiología , Animales , Ácidos Grasos , Agua Dulce
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