High-throughput characterization of the expressed antibiotic resistance genes in sewage sludge with transcriptional analysis.

Antibiotic resistance genes (ARGs) are emerging micro-pollutants that pose potential threats to environments and humans. Sewage sludge from wastewater is an important source for ARGs and current studies mainly focus on their existence in microbial genomes. However, little is known about which ARGs are expressed even though ARGs expression remains a better proxy for functional activity. In this study, the expressed ARGs in sewage sludge were characterized by high-throughput quantitative PCR (296 primer sets) combined with transcriptional analysis. A total of 202 ARG transcripts were detected and their abundances ranged from 3.1 × 109 to 1.2 × 1010 copies/g dry weight. The sum abundance of five most abundant ARG transcripts (qacEdelta1-02, sul2, qacEdelta1-01, aadA2-03, tetX) exhibited a linear correlation with the total abundance of ARG transcripts (R2 = 0.88, p < 10-4), suggesting that these genes could be regarded as indicators to quantitatively predict the total abundance of expressed ARGs. Dynamics of expressed ARGs were observed with lower abundances in summer and winter than those in other seasons (p < 0.05, Kruskal-Wallis test). Variation partitioning analysis indicated that the shift in bacterial community structures induced by changes in environmental attributes might be the main driver for the dynamics of expressed ARGs. Results of this study provided new insights into the ARGs in sewage sludge.

A review of the global climate change impacts, adaptation, and sustainable mitigation measures.

Climate change is a long-lasting change in the weather arrays across tropics to polls. It is a global threat that has embarked on to put stress on various sectors. This study is aimed to conceptually engineer how climate variability is deteriorating the sustainability of diverse sectors worldwide. Specifically, the agricultural sector's vulnerability is a globally concerning scenario, as sufficient production and food supplies are threatened due to irreversible weather fluctuations. In turn, it is challenging the global feeding patterns, particularly in countries with agriculture as an integral part of their economy and total productivity. Climate change has also put the integrity and survival of many species at stake due to shifts in optimum temperature ranges, thereby accelerating biodiversity loss by progressively changing the ecosystem structures. Climate variations increase the likelihood of particular food and waterborne and vector-borne diseases, and a recent example is a coronavirus pandemic. Climate change also accelerates the enigma of antimicrobial resistance, another threat to human health due to the increasing incidence of resistant pathogenic infections. Besides, the global tourism industry is devastated as climate change impacts unfavorable tourism spots. The methodology investigates hypothetical scenarios of climate variability and attempts to describe the quality of evidence to facilitate readers' careful, critical engagement. Secondary data is used to identify sustainability issues such as environmental, social, and economic viability. To better understand the problem, gathered the information in this report from various media outlets, research agencies, policy papers, newspapers, and other sources. This review is a sectorial assessment of climate change mitigation and adaptation approaches worldwide in the aforementioned sectors and the associated economic costs. According to the findings, government involvement is necessary for the country's long-term development through strict accountability of resources and regulations implemented in the past to generate cutting-edge climate policy. Therefore, mitigating the impacts of climate change must be of the utmost importance, and hence, this global threat requires global commitment to address its dreadful implications to ensure global sustenance.

Diversity, abundance and expression of the antibiotic resistance genes in a Chinese landfill: Effect of deposit age.

Landfills are the hotspots for the occurrence of antibiotic resistance genes (ARGs) in the environment. However, limited information is available on the profile of ARGs in response to the varying age of refuse in landfills. In this study, the diversity, abundance and expression of ARGs in a Chinese landfill were assessed by high-throughput quantitative PCR. A total of 154 ARGs were detected and 66% of them were transcriptionally active. The total abundance of ARG transcripts was one magnitude lower than that of ARGs. The ermT-01, tetX, sul2, aadA-02 and aadA2-03 genes were found to be the most abundant ARGs (ARG transcripts) and their sum abundance showed a linear relation with the total abundance of ARGs (ARG transcripts). The total abundance of ARGs (ARG transcripts) in young refuse was significantly higher than that in old refuse (p < 0.01) and the profile of ARGs (ARG transcripts) between the old and young refuse was distinct as revealed by the principal coordinates analysis. The variation partitioning analysis showed heavy metals (mainly Cr and Zn) were the major drivers that affect the profile of ARGs (ARG transcripts). These findings provided new insights into the ARGs in landfills and indicated their potential threats should not be neglected.

The potential applications of picotechnology in biomedical and environmental sciences.

Picotechnology development in vast disciplines is mainly attributed to the research and development (R and D) on nanotechnology. Being a parent technology, nanotechnology is the cornerstone of picotechnology. Like nanotechnology, the reference standard for picotechnology is nature, the cellular and subcellular functioning. Some studies have highlighted that the functional margin of similar type of molecules at picoscale (10-12) goes higher than at nanoscale (10-9). In this review, the potential applications of picotechnology have been evaluated especially in the disciplines of biomedical and environmental sciences. Extended surface area and improved electrical, chemical, optical, and mechanical properties make picotechnological products even better than nanomaterials. The fundamental objective of this study is to bring the attention of the scientific world towards the picoscale interventions and to highlight the wide scope of picotechnology as a newly emerging technology with applications in numerous sectors. Picotechnology has made it possible to measure very small structure in advance biomedical and environmental sciences studies. Adequate developments in picotechnology will certainly change human lives in near future because it will make possible for the research world to dive into systems and structures on picoscale. It will render a platform through which explorers can travel into ultra-small areas, which will lead to the creation of new dimensions as well as new opportunities. Eventually, in future, the picotechnology will become smaller enough to give birth to femtotechnology (10-15) in real-world applications.