RESUMEN
This study aimed to confirm that vertical transmission of hepatitis B virus (HBV) can occur via the infected ovum. Specimens studied were obtained from discarded test-tube embryos from mothers with chronic HBV infection who had received in vitro fertilization treatment. Single-cell reverse transcriptase-polymerase chain reaction was used to detect HBV mRNA in the embryos. HBV mRNA was detected in the cleavage embryos of patients with chronic HBV infection, with a detection rate of 13.2% (5/38). The level of serum HBV DNA was not related to the HBV mRNA positivity rates in embryos. In this study, HBV mRNA was detected in test-tube embryos from HBV-infected mothers who had received in vitro fertilization treatment. This confirms the theory of vertical transmission of HBV via the ovum, thereby providing an important theoretical basis for further study on the mechanism of HBV vertical transmission, influencing factors and blocking measures.
Asunto(s)
Virus de la Hepatitis B/aislamiento & purificación , Hepatitis B/transmisión , Transmisión Vertical de Enfermedad Infecciosa , Óvulo/virología , ARN Mensajero/metabolismo , ADN Complementario , ADN Viral/aislamiento & purificación , Técnicas de Cultivo de Embriones , Femenino , Fertilización In Vitro , Hepatitis B/virología , Virus de la Hepatitis B/genética , Humanos , Embarazo , ARN Mensajero/genética , Reacción en Cadena de la Polimerasa de Transcriptasa InversaRESUMEN
Objective:Imaging findings and treatment of sharp foreign bodies penetrating the esophagus to migrate in the neck were collected. Method:All of 9 cases were determined by CT imaging. The foreign bodies were removed in 8 cases through the lateral cervical approach. The last case was taken out by bronchoscope. Eight cases successfully removed the foreign bodies. No definite foreign body was found in 1 case. Result:There were no postoperative complications in 8 patients that successful removed of foreign bodies. All patients begin to eat normally after operation and were discharged from hospital in 7 days. Tracheoesophageal fistula occurred that happened to the patient that no foreign body was found in operation. So gastric tube was retained for 7 days. Conclusion:CT imaging should be performed to determine whether there is the possibility of foreign body penetrating out of the esophagus, for the patients who had explicit history but the foreignbodies could not be found by barium meal examination. If necessary, three-dimensional CT reconstruction of the neck should be performed to locate the foreign body. Surgical exploration should be done as soon as possible to avoid aimless migration of foreign bodies, and it is difficult to find it during operation.
Asunto(s)
Esófago , Cuerpos Extraños , Cuello , Fístula Traqueoesofágica , Broncoscopios , HumanosRESUMEN
Nasal glioma is a rare congenital malformation in maxillofacial region. It is sluggish, benign, non-tumorous and with low recurrence rate. It is important to evaluate whether there is any intracranial connection before the operation through CT or MRI to avoid the serious intracranial complications. We reported a case of a adult with a nasal glioma in sphenoid sinus, and discuss clinical characteristics, diagnosis, and pathology.