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Virus Genes ; 51(1): 136-9, 2015 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-25956292

RESUMEN

Bats are natural hosts of many zoonotic viruses. Monitoring bat viruses is important to detect novel bat-borne infectious diseases. In this study, next generation sequencing techniques and conventional PCR were used to analyze intestine, lung, and blood clot samples collected from wild bats captured at three locations in Davao region, in the Philippines in 2012. Different viral genes belonging to the Retroviridae and Herpesviridae families were identified using next generation sequencing. The existence of herpesvirus in the samples was confirmed by PCR using herpesvirus consensus primers. The nucleotide sequences of the resulting PCR amplicons were 166-bp. Further phylogenetic analysis identified that the virus from which this nucleotide sequence was obtained belonged to the Gammaherpesvirinae subfamily. PCR using primers specific to the nucleotide sequence obtained revealed that the infection rate among the captured bats was 30 %. In this study, we present the partial genome of a novel gammaherpesvirus detected from wild bats. Our observations also indicate that this herpesvirus may be widely distributed in bat populations in Davao region.


Asunto(s)
Quirópteros/virología , ADN Viral/química , ADN Viral/genética , Gammaherpesvirinae/clasificación , Gammaherpesvirinae/aislamiento & purificación , Infecciones por Herpesviridae/veterinaria , Animales , Análisis por Conglomerados , Gammaherpesvirinae/genética , Infecciones por Herpesviridae/virología , Datos de Secuencia Molecular , Filipinas , Filogenia , Reacción en Cadena de la Polimerasa , Análisis de Secuencia de ADN , Homología de Secuencia
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