Streptomyces sp. M54: an actinobacteria associated with a neotropical social wasp with high potential for antibiotic production.

Streptomyces symbionts in insects have shown to be a valuable source of new antibiotics. Here, we report the genome sequence and the potential for antibiotic production of "Streptomyces sp. M54", an Actinobacteria associated with the eusocial wasp, Polybia plebeja. The Streptomyces sp. M54 genome is composed of a chromosome (7.96 Mb), and a plasmid (1.91 Kb) and harbors 30 biosynthetic gene clusters for secondary metabolites, of which only one third has been previously characterized. Growth inhibition bioassays show that this bacterium produces antimicrobial compounds that are active against Hirsutella citriformis, a natural fungal enemy of its host, and the human pathogens Staphylococcus aureus and Candida albicans. Analyses through TLC-bioautography, LC-MS/MS and NMR allowed the identification of five macrocyclic ionophore antibiotics, with previously reported antibacterial, antitumor and antiviral properties. Phylogenetic analyses placed Streptomyces sp. M54 in a clade of other host-associated strains taxonomically related to Streptomyces griseus. Pangenomic and ANI analyses confirm the identity of one of its closest relatives as Streptomyces sp. LaPpAH-199, a strain isolated from an ant-plant symbiosis in Africa. In summary, our results suggest an insect-microbe association in distant geographic areas and showcase the potential of Streptomyces sp. M54 and related strains for the discovery of novel antibiotics.

Isolation of major components from the roots of Godmania aesculifolia and determination of their antifungal activities.

From the methanol root extract of Godmania aesculifolia, a species selected in a multinational OAS program aimed at discovering antifungal compounds from Latin American plants, a new chavicol diglycoside (1), the known 3,4-dihydroxy-2-(3-methylbut-2-en-1-yl)-3,4-dihydronaphthalen-1(2H)-one (2), and lapachol (3) were isolated and characterized by 1D and 2D NMR and MS techniques. Only 3 exhibited fairly good activity against a panel of clinical isolates of Cryptococcus neoformans (MIC50 between 7.8 and 31.2 µg/mL) and moderate activities against Candida spp. and non-albicans Candida spp.

Conhecimento da equipe de enfermagem sobre reanimação cardiopulmonar antes e após capacitação / Conocimiento del equipo de enfermería sobre reanimación cardiopulmonar antes y después de capacitación / Knowledge of the nursing team on cardiopulmonary resuscitation before and after training

Objetivo: avaliar o conhecimento dos profissionais da equipe de enfermagem no atendimento à parada cardiorrespiratória e comparar antes e após capacitação. Método: pesquisa prospectiva e comparativa, realizada em um hospital público em São Paulo, em 2021, com 68 profissionais. Foi aplicado um questionário sobre parada cardiorrespiratória antes e após capacitação, para comparação. Resultados: a maioria dos profissionais reconhece os sinais clássicos de parada cardiorrespiratória e a sequência correta de atendimento, todavia, teve dificuldade em identificar os ritmos chocáveis e as ações após a desfibrilação. A média de acertos antes e após a realização da capacitação foi de 43,65 e 66,11, respectivamente, e corrobora com a eficácia da ação educativa. Conclusão: é extremamente relevante ofertar, sistematicamente, capacitações à equipe, de modo a garantir uma assistência mais segura e eficaz.

Objetivo: evaluar el conocimiento de los profesionales del equipo de enfermería en la atención a la parada cardiorrespiratoria y comparar antes y después de capacitación. Método: investigación prospectiva y comparativa, realizada en un hospital público en São Paulo, en 2021, con 68 profesionales. Se aplicó un cuestionario sobre parada cardiorrespiratoria antes y después de capacitación, para comparación. Resultados: la mayoría de los profesionales reconocen los signos clásicos de parada cardiorrespiratoria y la secuencia correcta de atención, sin embargo, tuvo dificultad en identificar los ritmos chocables y las acciones después de la desfibrilación. El promedio de aciertos antes y después de la realización de la capacitación fue de 43,65 y 66,11, respectivamente, y corrobora con la eficacia de la acción educativa. Conclusión: es extremadamente relevante ofrecer, sistemáticamente, capacitaciones al equipo, de modo a garantizar una asistencia más segura y eficaz.

Objective: to evaluate the knowledge of nursing staff professionals in the care of cardiorespiratory arrest and to compare before and after training. Method: prospective and comparative research, conducted in a public hospital in São Paulo, in 2021, with 68 professionals. A questionnaire on cardiorespiratory arrest before and after training was applied for comparison. Results: most professionals recognize the classic signs of cardiorespiratory arrest and the correct sequence of care, however, had difficulty identifying the shockable rhythms and actions after defibrillation. The average number of correct answers before and after the training was 43.65 and 66.11, respectively, and corroborates the effectiveness of the educational action. Conclusion: it is extremely relevant to systematically offer training to the team in order to ensure safer and more effective care.