Synergy between polypyrrol and benzoic acid against antibiotic-resistant Salmonella spp.

AIMS: The purpose was to characterize Salmonella Heidelberg (SH) and Minnesota (SM) isolates in terms of their resistance and persistence profile and to assess the antimicrobial effect of benzoic acid (BA) and polypyrrole (PPy). METHODS AND RESULTS: The 20 isolates from broiler litter drag swabs were submitted to antibiogram and efflux pump expression. The minimum inhibitory/bactericidal concentration (MIC/MBC) of the compounds, synergistic activity, time kill, biofilm production, presence of related genes, and molecular docking between compounds and bacterial target sites were evaluated. All isolates showed multidrug resistance (MDR) and BA and PPy showed mean MIC (1750 and 342 µg ml-1) and MBC (3167 and 1000 µg ml-1), respectively. None of the isolates expressed an efflux pump. The compounds showed synergism against an SH isolate and reduced the count by 3 logs in the presence of the compounds after 4 h. Most isolates (16/20) produced weak to moderate biofilm and 17 showed genes related to biofilm. The compounds interacted with two essential proteins, 3,4-dihydroxy-2-butanone 4-phosphate synthase proteins and ferritin-like domain-containing protein, in bacterial metabolism at different target sites. CONCLUSIONS: It can be concluded that BA and PPy showed activity on SH and SM, MDR, and biofilm producers, with a potential synergistic effect.

Dietary Intake of Flaxseed Oil since Early Stages of Life Promotes Femur Quality in Male Rats.

BACKGROUND: Flaxseed oil (FO) is an alpha linolenic acid source important for growth and body development. However, there is little literature on the role of FO in critical stages of bone development and formation. OBJECTIVE: This study evaluated the influence of a diet containing FO on rat femurs. METHODS: After birth, mothers and pups were divided into control and flaxseed groups (n = 6 pups each) fed diets containing 7% soybean oil (C) or 7% FO. At 21 days, pups were weaned and separated from the mothers, and control or experimental diets were continued. At 67 days, the following were analyzed: osteocalcin and osteoprotegerin (OPG) levels, bone mineral density (BMD) and content, and bone area; the dimension, BMD, head radiodensity, and biomechanical proprieties of the right femur; and histomorphometric parameters of the left femur. RESULTS: Compared to the C group, the FO group presented (p < 0.05) a lower body mass (-3.7%) and medullary area (-10.1%) and higher osteocalcin (+36.7%), OPG (+52.5%), femur width (+3.8%), absolute mass (+2.3%), femur BMD (+3.6%), head radiodensity (+6.1%), maximum force (+7.4%), breaking strength (+17.3), and cortical thickness (+7.0). CONCLUSION: The FO diet contributed to femur quality in healthy male Wistar rats.

The influence of low-fidelity simulator training on canine peripheral venous puncture procedure.

BACKGROUND AND AIM: Blood collection from dogs is the most commonly performed procedure in the medical clinic. However, different factors can interfere with the quality of the material collected, potentially causing complications for patients. Simulated skill training is a teaching strategy designed to provide early training to students, develop their skills and self-confidence, and increase the procedure's success while reducing complications. Therefore, the aim of this study was to evaluate skill training using a low-fidelity simulator in the peripheral venipuncture procedure and examine the training's influence on the in vivo procedure. MATERIALS AND METHODS: To assess skill training, this study used a low-fidelity simulator in the peripheral venipuncture procedure and examines the training's effect on the in vivo procedure. In total, 100 dogs, 65 undergraduate students, 3 veterinarians, and 4 previously trained evaluators participated. The canine in vivo venipuncture procedure was evaluated both before and after the simulated skill training and the low-fidelity simulator training. Data were collected on participants' self-confidence levels. RESULTS: Local complications occurred during in vivo practice; however, after training, they decreased. Gloves were more frequently used during the procedure, resulting in a reduction of both harvest attempts and complications, as well as increased levels of self-confidence in post-training participants. The simulator developed had low fidelity, low cost, and was easy to create. CONCLUSION: Skill training in peripheral venipuncture using a low-fidelity simulator positively influences student learning, increases their self-confidence during in vivo harvesting, and reduces the complications of the procedure, improving patient well-being.

Análise do marketing dos produtos alimentícios infantís / Marketing analysis of children's food products

Objetivo ­ Analisar o marketing nos produtos alimentícios destinados ao público infantil. Métodos ­ O método de abordagem utilizado foi de observação, hipotético dedutivo, de forma quantitativa, dos produtos voltados para o público infantil, comercializados em hipermercados (representados por Y¹ e Y²), em mercado de bairro (representados por X¹ e X²) e atacadistas (representados por Z¹ e Z²), no município de Taubaté/SP. Cada produto analisado foi classificado em sete categorias. Resultados ­ Foi feita a observação de 432 produtos que possuem em seus rótulos personagens ou desenhos infantis, sendo 419 produtos industrializados e 13 produtos naturais. Foram encontrados 30,09% de guloseimas; 27,54% de produtos lácteos; 15,5% de biscoito recheado; 14,81% salgadinho de milho; 9,02% de suco de fruta; 1,38% de frutas e 1,62% produtos integrais. Conclusão ­ Ao analisar quantos produtos naturais e industrializados fazem uso do mesmo personagem, não foi encontrado nenhum em comum. No momento da coleta dos dados foi possível observar que os produtos que possuem um marketing mais infantilizado estão dispostos em prateleiras mais baixas nos mercados de grande e médio porte, já nos mercados de pequeno porte estes produtos nem sempre estão em prateleiras baixas.

Objective ­ To analyze the marketing of food products related to children. Methods ­ The method of approach used for observation, hypothetical deductive, quantitative form, products aimed at children, marketed in hypermarkets (represented by Y and Y²), neighborhood market (represented by X and X²) and wholesalers (represented Z¹ and Z²), in the municipality of Taubaté / SP. Each product analyzed was classified into seven categories. Results ­ An observation was made of 432 products that have on their labels characters or children's drawings, being 419 processed products and 13 natural products. We found 30.09% of fat, 27.54% of dairy products, 15.5% of stuffed crackers, 14.81% of corn chips, 9.02% of fruit juice, 1.38% of fruits and 1, 62% whole products. Conclusion ­ When analyzing how many natural and industrialized products make use of the same character, none were found in common. At no time in the data collection was it possible to observe that products that have a more childish marketing are arranged on the lower shelves in the medium and large markets, whereas in the small markets these products are not always on the lower shelves

ATIVIDADE FÍSICA E AMBIENTE: A INFLUÊNCIA DOS Parques verdes urbanos NA SAÚDE / Physical activity and environment: the influence of urban green spaces on health / Actividad física y ambiente: la influencia de los parques verdes urbanos en la salud

ABSTRACT Introduction: Urban green spaces can offer opportunities for regular physical activity, providing benefits in the lives of members of the population, such as physical, social and affective health. The aim of this article consists of analyzing the influence of urban green spaces on physical activity, focusing on urban environmental quality and the health of those who engage in physical activities in open areas. The study has a multidisciplinary approach from the perspective of environmental health. Method: The study design was based on qualitative research, using a bibliographic and document search as well as field surveys, whose technique involved direct observation (DO). The sample selection was based on Miot (2011), consisting of a total of 36 individuals aged between 18 and 40 years, who perform physical activities regularly, at least three times a week, for at least 50 minutes. Questionnaires were also applied using the IPAQ (assessment of physical activity) and Borg Scale parameters. Results: The study showed that the research participants became more active, possibly being stimulated and motivated by their surroundings with widespread vegetation cover, the good infrastructure of the venue and the guidance provided during the research. Conclusion: The research project showed that the greater the frequency and intensity of physical activity in open areas, the better the body's physiological adaptability. Level of Evidence II; Prognostic study.

RESUMO Introdução: Os parques verdes urbanos podem oferecer oportunidades para a prática regular de atividade física, proporcionando benefícios para a vida da população, como por exemplo, saúde física, social e afetiva. O objetivo do presente artigo consiste em analisar a influência dos parques verdes urbanos na prática de atividades físicas, focando-se na qualidade ambiental urbana e na saúde dos praticantes de atividades físicas em áreas abertas. O estudo tem uma abordagem multidisciplinar segundo a perspectiva da saúde ambiental. Método: O projeto de estudo baseou-se em pesquisa qualitativa, utilizando pesquisa bibliográfica e documental e levantamentos de campo, cuja técnica contou com a observação direta (DO). A seleção da amostra foi baseada em Miot (2011), contando com36 indivíduos com idade entre 18 e 40 anos que praticam regularmente atividades físicas, pelo menos, três vezes por semana, por no mínimo 50 minutos. Também foram realizados questionários utilizando o IPAC (nível de atividade física avaliado) e os parâmetros da Escala de Borg. Resultados: O estudo mostrou que os participantes da pesquisa se tornaram mais ativos, possivelmente, sendo estimulados e motivados pelo ambiente com grande presença de vegetação, a boa infraestrutura do local e as orientações feitas durante a pesquisa. Conclusão: A pesquisa mostrou que quanto maior a frequência e a intensidade da atividade física em áreas abertas, melhor é a adaptabilidade fisiológica do organismo. Nível de evidência II; Estudo prognóstico.

RESUMEN Introducción: Los parques verdes urbanos pueden ofrecer oportunidades para la práctica regular de actividad física, proporcionando beneficios para la vida de la población, como por ejemplo, salud física, social y afectiva. El objetivo del presente artículo consiste en analizar la influencia de los parques verdes urbanos en la práctica de actividades físicas, enfocándose en la calidad ambiental urbana y la salud de los practicantes de actividades físicas en áreas abiertas. El estudio tiene un abordaje multidisciplinario según la perspectiva de la salud ambiental. Método: El proyecto de estudio se basó en la investigación cualitativa, mediante búsqueda bibliográfica y documental, y levantamientos de campo, cuya técnica contó con la observación directa (OD). La selección de la muestra se basó en Miot (2011), contando con 36 individuos con edad entre 18 y 40 años, que practican regularmente actividades físicas, al menos tres veces por semana, durante al menos 50 minutos. También fueron realizados cuestionarios utilizando el IPAC (nivel de actividad física evaluado) y los parámetros de la Escala de Borg. Resultados: El estudio mostró que los participantes de la investigación se volvieron más activos, posiblemente siendo estimulados y motivados por el ambiente con gran presencia de vegetación y la buena infraestructura del local y las orientaciones realizadas durante la investigación. Conclusión: La investigación mostró que cuanto mayor es la frecuencia e intensidad de la actividad física en áreas abiertas, mejor es la adaptabilidad fisiológica del organismo. Nivel de evidencia II; Estudio pronóstico.