Detection of bone particles in non-carbonate soils.

A routine geologic test for the presence of carbonate minerals in soil is the application of dilute hydrochloric acid (HCl) and observation for an effervescent reaction. This study tests whether non-carbonate soils can exhibit effervescence in the presence of HCl if bone particles are present in the soil. Five bone fragments displaying various taphonomic alterations were ground and sieved to achieve uniform particle size fractions. A non-carbonate soil was mixed with each bone particle fraction, and 1 molar HCl solution was applied while observing the reaction using a stereo microscope. All tests resulted in the effervescence of bone particles, which could be easily located within the soil based on the presence of small bubbles surrounding the bone particle. These results show that a simple, quick, cost-effective test can be used to presumptively determine whether soil may contain bone particles, even if they are too small to be identified morphologically. Results also suggest that, since bone particles in soil can produce the same type of effervescence expected in carbonate soil, additional tests may be needed to determine the source of an effervescent reaction. These findings also highlight the insight that can be gained through interdisciplinary discussions and investigations.

Soil survey laboratory grain count data to substantiate the rarity of mineral grains in forensic soil reports of examination.

The Natural Resources Conservation Service-Kellogg Soil Survey Laboratory has a large publicly available database of laboratory analyses of soil horizons collected from soil profiles largely from the United States. Among these soil properties are mineral grain counts from selected sand and silt fractions of soil horizons, performed by polarized light microscopy (PLM). These grain counts of over 20,000 fractions from 7534 sites provide a substantial reference that a forensic soil examiner could use to substantiate the rarity or commonness of a mineral species. The statement of the rarity or commonness of various minerals provide juries with additional context for the interpreting the results of a forensic soil comparison within the framework of a trial. The grain count data at specific locations can also be assessed to aid in soil provenance investigations, for cases where there are grain-counted sites in relevant locations. Two examples of application of these to data to soil evidence are included, one relating soil the rarity of a mineral (andalusite) to provide context in a soil comparison and one to aid in narrowing target regions in a soil provenance investigation.

Strengths, Limitations, and Recommendations for Instrumental Color Measurement in Forensic Soil Characterization.

Color determination of soil evidence is often done by visual comparison to soil color charts. A handheld spectrophotometer was tested with representative materials for its suitability for forensic soil characterization. Instrumental colorimetry provides accurate colorimetry with ~10-fold better precision than a soil color chart. The minimum sample size for accurate color determination was between 0.02 and 0.04 mg of fine soil for the specific instrument tested. Reporting colors in the L*a*b* space permits quantification of ΔE00 , a measure of perceptible color difference, could enable objective quantification of small color differences and thresholds for forensic soil comparisons. A ΔE00 greater than ~ 3.5 to 6 likely indicates disparate soil sources in a forensic comparison, in the absence of confounding factors like sample alteration. Despite the superior precision of instrumental colorimetry, this approach is inappropriate for samples which are mottled at an inseparable scale, attached to a substrate, or too small for instrumental measurement.