Extracellular HSP90 promotes differentiation of lens epithelial cells to fiber cells by activating LRP1-YAP-PROX1 axis.

Capsular residual lens epithelial cells (CRLEC) undergo differentiation to fiber cells for lens regeneration or tansdifferentiation to myofibroblasts leading to posterior capsular opacification (PCO) after cataract surgery. The underlying regulatory mechanism remains unclear. Using human lens epithelial cell lines and the ex vivo cultured rat lens capsular bag model, we found that the lens epithelial cells secrete HSP90α extracellularly (eHSP90) through an autophagy-associated pathway. Administration of recombinant GST-HSP90α protein or its M-domain induces the elongation of rat CRLEC cells with concomitant upregulation of the crucial fiber cell transcriptional factor PROX1and its downstream targets, ß- and γ-crystallins and structure proteins. This regulation is abolished by PROX1 siRNA. GST-HSP90α upregulates PROX1 by binding to LRP1 and activating LRP1-AKT mediated YAP degradation. The upregulation of GST-HSP90α on PROX1 expression and CRLEC cell elongation is inhibited by LRP1 and AKT inhibitors, but activated by YAP-1 inhibitor (VP). These data demonstrated that the capsular residue epithelial cells upregulate and secrete eHSP90α, which in turn drive the differentiation of lens epithelial cell to fiber cells. The recombinant HSP90α protein is a potential novel differentiation regulator during lens regeneration.

When biochar is involved in rhizosphere dissipation and plant absorption of pesticides: A meta-analysis.

Clarifying the influences of biochar input on the rhizosphere dissipation and plant absorption of pesticides is a crucial prerequisite for utilizing biochar in the restoration of pesticide-contaminated soils. Nevertheless, the application of biochar to pesticide-contaminated soils does not always achieve consistent results on the rhizosphere dissipation and plant absorption of pesticides. Under the new situation of vigorously promoting the application of biochar in soil management and carbon sequestration, a timely review is needed to further understand the key factors affecting biochar remediation of pesticide-contaminated soil. In this study, a meta-analysis was conducted utilizing variables from three dimensions of biochar, remediation treatment, and pesticide/plant type. The pesticide residues in soil and the pesticide uptake by plant were used as response variables. Biochar with high adsorption capacity can impede the dissipation of pesticides in soil and mitigate their absorption by plants. The specific surface area of biochar and the type of pesticide are critical factors that affect pesticide residues in soil and plant uptake, respectively. Applying biochar with high adsorption capacity, based on specific dosages and soil characteristics, is recommended for the remediation of continuously cultivated soil contaminated with pesticides. This article aims to provide a valuable reference and understanding for the application of biochar-based soil remediation technology and the treatment of pesticide pollution in soil.

Managing Fenton-treated sediment with biochar and sheep manure compost: Effects on the evolutionary characteristics of bacterial community.

Fenton oxidation is a widely used method for the fast and efficient treatment of contaminated sediment, but few studies have investigated the management of Fenton-treated sediment for resource utilization. In this study, the evolutionary characteristics of bacterial community composition in Fenton-treated riverine sediment were investigated using 16S rRNA gene sequencing after the incorporation of rice straw biochar and sheep manure compost. The Fenton treatment caused a decline in the relative abundance of Bacteroidetes from 39% to 8% on the 7th day, and using biochar and compost rapidly increased the relative abundance of Firmicutes from 13% to 61% and 57%, respectively. Applying 1.25 wt% biochar after the Fenton treatment contributed to high Shannon diversity indices of 4.80, 4.69, and 4.76 on the 7th, 28th, and 56th day, respectively. The reduced differences of Shannon indexes on the 56th day indicated that the bacterial diversity among different treatments tended to be similar over time. The genera Flavisolibacter and Bacillus were representatively detected on the 7th day in the untreated sediment and Fenton/biochar-treated sediment, respectively. The number of feature bacteria decreased significantly from 88 on the 7th day to 29 on the 56th day. The community functions for the carbon, nitrogen, and sulfur cycles were sensitive to the Fenton-treatment and the subsequent treatment with biochar and compost. This study may provide a useful reference for follow-up work on the remediation of contaminated sediment using advanced oxidation processes, and promote the development of resource utilization of amended sediment.

The engineered expression of secreted HSPB5-Fc in CHO cells exhibits cytoprotection in vitro.

BACKGROUND: HSPB5 is an ATP-independent molecular chaperone that is induced by heat shock or other proteotoxic stresses. HSPB5 is cytoprotective against stress both intracellularly and extracellularly. It acts as a potential therapeutic candidate in ischemia-reperfusion and neurodegenerative diseases. RESULTS: In this paper, we constructed a recombinant plasmid that expresses and extracellularly secrets a HSPB5-Fc fusion protein (sHSPB5-Fc) at 0.42 µg/ml in CHO-K1 cells. This sHSPB5-Fc protein contains a Fc-tag at the C-terminal extension of HSPB5, facilitating protein-affinity purification. Our study shows that sHSPB5-Fc inhibits heat-induced aggregation of citrate synthase in a time and dose dependent manner in vitro. Administration of sHSPB5-Fc protects lens epithelial cells against cisplatin- or UVB-induced cell apoptosis. It also decreases GFP-Httex1-Q74 insolubility, and reduces the size and cytotoxicity of GFP-Httex1-Q74 aggregates in PC-12 cells. CONCLUSION: This recombinant sHSPB5-Fc exhibits chaperone activity to protect cells against proteotoxicity.

Double-charged self-assembled rGO/g-C3N4 membrane prepared by "functional group substitution" for heavy metal ions rejection at low pressure.

Heavy metals are still the critical pollutants in industrial wastewater and there is an urgent need for efficient and environmentally friendly treatment technologies. Reduced graphene oxide (rGO) is widely used for preparations of nanofiltration (NF) membranes but suffers from poor hydrophilicity and electronegativity. In this work, a double-charged rGO/g-C3N4-P membrane was prepared for removal of heavy metals at low pressure. Graphitic carbon nitride (g-C3N4) assisted reduction of GO membranes under ultraviolet (UV) irradiation, and the modification of functional groups with high polarity improved the hydrophilicity of membrane surface. The filtration performance for heavy metals of rGO/g-C3N4-P membrane was evaluated under low pressure (1-2 bar). The rejection rates of Cu2+, Cr3+, Mn2+, Cd2+, and Pb2+ by membranes reduced by UV for 18 h (rGO/g-C3N4-18-P membrane) reached 94.72 %, 98.05 %, 82.32 %, 88.2 % and 77.15 %, respectively. In the experiment of mixed simulated wastewater, the rejection rates of NO3- and SO42- both reached >95 %. Outstanding rejection rates were attributed to the interaction and the synergy effect of double-charged layers as well as steric effects. Meanwhile, the water flux of rGO/g-C3N4-18-P membrane was as high as 37.14-50.16 L m-2h-1bar-1 (1-2 bar). The high flux was due to the reduced degree of oxidation so that water molecules transported between GO nanochannels more smoothly and the transport path was shortened through the nanopores of g-C3N4. Obviously, flux and heavy metal rejection of rGO/g-C3N4-18-P membrane were simultaneously improved, breaking "trade-off" effect. Furthermore, rGO/g-C3N4-18-P membrane showed excellent antifouling ability and the potential for heavy metal wastewater filtration in comparison with other NF membranes reported in literature.

Effects of activated carbon, biochar, and carbon nanotubes on the heterogeneous Fenton oxidation catalyzed by pyrite for ciprofloxacin degradation.

Pyrite and engineering carbon materials have received increasing attention for their catalytic potential in Fenton reactions due to their extensive sources and low cost. However, effects of carbon materials on the degradation of pollutants by pyrite-catalyzed heterogeneous Fenton oxidation have not been fully understood. In this study, the performance of pyrite-catalyzed heterogeneous Fenton system on the degradation of ciprofloxacin (CIP) was investigated in the presence of activated carbon (AC), biochar (BC), and carbon nanotubes (CNTs). Synchronous and asynchronous experiments (adsorption and catalysis) were conducted to elucidate the roles of the carbon materials in pyrite-catalyzed Fenton reactions. The results demonstrated that all the three carbon materials accelerated the pyrite-catalyzed Fenton oxidation of CIP. Under the experimental conditions, the reaction rates, which were obtained by fitting the synchronous experimental results with the pseudo-first-order kinetic model, of pyrite/AC, pyrite/BC and pyrite/CNTs with H2O2 for the removal of CIP were 8.28, 3.40 and 3.37 times faster than that of pyrite alone. Adsorption experiments and characterization analysis showed that AC had a higher adsorption capacity than BC and CNTs for CIP, which enabled it to distinguish itself in assisting the pyrite-catalyzed Fenton oxidation. In the presence of the carbon materials, the adsorption effect should not be neglected when studying the catalytic performance of pyrite. Free radical quenching experiments and electron spin-resonance spectroscopy (ESR) were used to detect and identify free radical species in the reactions. The results showed that hydroxyl radicals (•OH) contributed significantly to the degradation of CIP. The addition of carbon materials promoted the production of •OH, which favored the degradation of CIP. The results of this study suggested that the synergistic effect of oxidation and adsorption promoted the removal of CIP in pyrite/carbon materials/H2O2 systems, and coupling pyrite and carbon materials shows great potential in treating antibiotic wastewater.