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2.
Emerg Infect Dis ; 18(2): 290-3, 2012 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-22305204

RESUMEN

Francisella tularensis subsp. holarctica isolates from Austria, Germany, Hungary, Italy, and Romania were placed into an existing phylogeographic framework. Isolates from Italy were assigned to phylogenetic group B.FTNF002-00; the other isolates, to group B.13. Most F. tularensis subsp. holarctica isolates from Europe belong to these 2 geographically segregated groups.


Asunto(s)
Francisella tularensis/genética , Europa (Continente) , Francisella tularensis/clasificación , Tipificación Molecular , Filogenia , Filogeografía , Polimorfismo de Nucleótido Simple , Análisis de Secuencia de ADN
3.
Emerg Infect Dis ; 16(2): 238-43, 2010 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-20113553

RESUMEN

In November 2005, an outbreak of tularemia occurred among 39 participants in a hare hunt in Hesse, Germany. Previously reported tularemia outbreaks in Germany dated back to the 1950s. We conducted a retrospective cohort study among participants and investigated the environment to identify risk factors for infection. Ten participants had serologic evidence of acute Francisella tularensis infection; 1 other participant died before laboratory confirmation was obtained. Presence within 5 meters of the place where disemboweled hares were rinsed with a water hose was the risk factor most strongly associated with infection (risk ratio 22.1; 95% confidence interval 13.2-154.3). Swabs taken at the game chamber and water samples were PCR negative for F. tularensis. Eleven of 14 hare parts showed low-level concentrations of F. tularensis, compatible with cross-contamination. More than half of case-patients may have acquired infection through inhalation of aerosolized droplets containing F. tularensis generated during rinsing of infected hares.


Asunto(s)
Brotes de Enfermedades , Francisella tularensis , Exposición por Inhalación , Tularemia/epidemiología , Tularemia/transmisión , Zoonosis/transmisión , Adolescente , Adulto , Aerosoles , Anciano , Animales , Niño , Alemania/epidemiología , Liebres , Humanos , Masculino , Persona de Mediana Edad , Oportunidad Relativa , Reacción en Cadena de la Polimerasa , Estudios Retrospectivos , Pruebas Serológicas , Adulto Joven
4.
BMC Microbiol ; 10: 72, 2010 Mar 08.
Artículo en Inglés | MEDLINE | ID: mdl-20205957

RESUMEN

BACKGROUND: Francisella (F.) tularensis is the causative agent of tularemia. Due to its low infectious dose, ease of dissemination and high case fatality rate, F. tularensis was the subject in diverse biological weapons programs and is among the top six agents with high potential if misused in bioterrorism. Microbiological diagnosis is cumbersome and time-consuming. Methods for the direct detection of the pathogen (immunofluorescence, PCR) have been developed but are restricted to reference laboratories. RESULTS: The complete 23S rRNA genes of representative strains of F. philomiragia and all subspecies of F. tularensis were sequenced. Single nucleotide polymorphisms on species and subspecies level were confirmed by partial amplification and sequencing of 24 additional strains. Fluorescent In Situ Hybridization (FISH) assays were established using species- and subspecies-specific probes.Different FISH protocols allowed the positive identification of all 4 F. philomiragia strains, and more than 40 F. tularensis strains tested. By combination of different probes, it was possible to differentiate the F. tularensis subspecies holarctica, tularensis, mediasiatica and novicida. No cross reactivity with strains of 71 clinically relevant bacterial species was observed. FISH was also successfully applied to detect different F. tularensis strains in infected cells or tissue samples. In blood culture systems spiked with F. tularensis, bacterial cells of different subspecies could be separated within single samples. CONCLUSION: We could show that FISH targeting the 23S rRNA gene is a rapid and versatile method for the identification and differentiation of F. tularensis isolates from both laboratory cultures and clinical samples.


Asunto(s)
Francisella/clasificación , Hibridación Fluorescente in Situ/métodos , ARN Bacteriano/análisis , ARN Ribosómico 23S/análisis , Algoritmos , Animales , Bacteriemia/microbiología , Francisella/genética , Humanos , Hígado/microbiología , Ratones , Microscopía de Contraste de Fase , Modelos Genéticos , Filogenia , Polimorfismo de Nucleótido Simple , ARN Bacteriano/genética , ARN Ribosómico 23S/genética , Sensibilidad y Especificidad , Células U937
5.
BMC Infect Dis ; 8: 157, 2008 Nov 17.
Artículo en Inglés | MEDLINE | ID: mdl-19014635

RESUMEN

BACKGROUND: Tularemia re-emerged in Germany starting in 2004 (with 39 human cases from 2004 to 2007) after over 40 years of only sporadic human infections. The reasons for this rise in case numbers are unknown as is the possible reservoir of the etiologic agent Francisella (F.) tularensis. No systematic study on the reservoir situation of F. tularensis has been published for Germany so far. METHODS: We investigated three areas six to ten months after the initial tularemia outbreaks for the presence of F. tularensis among small mammals, ticks/fleas and water. The investigations consisted of animal live-trapping, serologic testing, screening by real-time-PCR and cultivation. RESULTS: A total of 386 small mammals were trapped. F. tularensis was detected in five different rodent species with carrier rates of 2.04, 6.94 and 10.87% per trapping area. None of the ticks or fleas (n = 432) tested positive for F. tularensis. We were able to demonstrate F. tularensis-specific DNA in one of 28 water samples taken in one of the outbreak areas. CONCLUSION: The findings of our study stress the need for long-term surveillance of natural foci in order to get a better understanding of the reasons for the temporal and spatial patterns of tularemia in Germany.


Asunto(s)
Francisella tularensis/aislamiento & purificación , Roedores/microbiología , Tularemia/epidemiología , Microbiología del Agua , Animales , Brotes de Enfermedades , Reservorios de Enfermedades/microbiología , Enfermedades Endémicas/estadística & datos numéricos , Francisella tularensis/genética , Alemania/epidemiología , Humanos , Recurrencia , Siphonaptera/microbiología , Garrapatas/microbiología , Tularemia/microbiología
6.
Int J Syst Evol Microbiol ; 60(Pt 8): 1887-1896, 2010 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-19783615

RESUMEN

Strain FhSp1T, isolated from human blood in Spain in 2003, was studied for its taxonomic allocation. By 16S rRNA and recA gene sequencing, the strain was shown to belong to the genus Francisella. In the 16S rRNA gene sequence, Francisella sp. FhSp1T shared similarity of more than 99% with strains of Francisella tularensis subspecies and Francisella novicida U112T, 98% with Francisella piscicida GM2212T and 98.4% with Francisella philomiragia ATCC 25015T. In the recA gene sequence, Francisella sp. FhSp1T exhibited 91.6-91.7% similarity to strains of F. tularensis subspecies, 91.2% to F. novicida U112T and 84% to F. philomiragia ATCC 25017. The genus affiliation was supported by a quinone system typical of Francisella (Q-8 as the major component), a complex polar lipid profile similar to that of F. tularensis with the major components diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine and an unknown aminophospholipid (APL4) and a fatty acid profile consisting mainly of C10:0 (17.2%), C14:0 (11.2%), C16:0 (13.1%), C18:0 3-OH (14.2%) and C18:1omega9c (7.1%). DNA-DNA hybridization, which showed unambiguously that FhSp1T represents a novel species, and the results of biochemical tests allowed genotypic and phenotypic differentiation of the isolate from all hitherto-described Francisella species. A multiplex PCR developed in the course of this study discriminated FhSp1T from representatives of all other Francisella species and subspecies, clades A.I and A.II of F. tularensis subsp. tularensis and F. tularensis subsp. holarctica biovar japonica and also between these representatives of the genus. Therefore, we propose the name Francisella hispaniensis sp. nov., with the type strain FhSp1T (=FnSp1T =FSC454T =F62T =DSM 22475T =CCUG 58020T). Furthermore, we formally propose the transfer of the species Francisella novicida to the species Francisella tularensis as Francisella tularensis subsp. novicida comb. nov. (type strain ATCC 15482T =CCUG 33449T =CIP 56.12T). We also present an emended description of the genus Francisella.


Asunto(s)
Sangre/microbiología , Francisella/clasificación , Francisella/aislamiento & purificación , Infecciones por Bacterias Gramnegativas/microbiología , ADN Bacteriano/genética , ADN Ribosómico/genética , Ácidos Grasos/metabolismo , Francisella/genética , Francisella/metabolismo , Humanos , Datos de Secuencia Molecular , Filogenia , ARN Ribosómico 16S/genética
7.
Mol Cell Probes ; 21(1): 12-6, 2007 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-16893624

RESUMEN

Tularemia is a plague-like infection caused by Francisella (F.) tularensis classified as a biological warfare agent. F. tularensis subsp. tularensis is the most virulent subspecies demanding rapid diagnosis. Typing systems for this fastidious bacterium to the subspecies level are laborious and time consuming. Therefore, the aim of this study was to develop a real-time PCR for the rapid and specific identification of F. tularensis subsp. tularensis. The specificity of the assay was determined using a comprehensive panel of Francisella strains, clinically relevant bacteria, and DNA preparations of potential hosts. F. tularensis subsp. tularensis was specifically detected but no other organisms. The range of linearity was determined to be 100 fg to 10 ng, the lower limit of detection was 25 fg of DNA (13 genome equivalents). An internal amplification control PCR system targeting lambda phage DNA was included. Neither the internal amplification control nor host DNA influenced the cycle threshold values obtained for F. tularensis subsp. tularensis. In conclusion, we have developed a highly sensitive and specific assay that can be integrated into real-time PCR-based identification procedures for biological agents. This is a major diagnostic improvement, as all other methods for the specific identification of F. tularensis subsp. tularensis are more time consuming.


Asunto(s)
Sondas de ADN/genética , Francisella tularensis/genética , Francisella tularensis/aislamiento & purificación , Reacción en Cadena de la Polimerasa/métodos , Animales , Cartilla de ADN , ADN Bacteriano/análisis , ADN Bacteriano/genética , Francisella tularensis/clasificación , Humanos , Hibridación de Ácido Nucleico , Análisis de Regresión , Sensibilidad y Especificidad , Factores de Tiempo
9.
Intervirology ; 45(4-6): 267-74, 2002.
Artículo en Inglés | MEDLINE | ID: mdl-12566709

RESUMEN

OBJECTIVE: A number of trials in primates using a wide range of putative vaccines based on simian immunodeficiency virus (SIV) have been performed and are summarised here. METHODS: Rhesus macaques and African green monkey (AGMs) were immunised with the test vaccines and challenged with live virus to test the efficacy of the induced or transferred immune responses to protect from infection or disease development. RESULTS: In initial studies, successful protection from challenge by whole inactivated virus vaccines was subsequently shown to be mediated by immune responses to human cell rather than viral proteins. Passive transfer of neutralising antibodies failed to protect against challenge. The induction of SIV-specific cytotoxic T lymphocytes (CTLs) using lipopeptides also failed to protect from infection, and whereas the frequency of post-infection CTLs (as measured by limiting dilution CTL assay and MHC/tetramer staining) correlated inversely with the cell-associated virus load, no correlation with the plasma virus load was observed. No immunological correlation of protection could be identified in macaques immunised with live attenuated SIV, with sterilising immunity being induced as early as 10 weeks after infection with the attenuated virus. Similarly, whole inactivated virus and passive IgG transfer failed to protect the natural host AGM species from challenge with apathogenic SIVagm, although live attenuated SIVagm afforded some protection despite the lack of overt vaccine virus replication. CONCLUSIONS: 'Traditional' types of vaccine are either ineffective or inappropriate for use in humans. Current efforts are therefore focusing on the rapidly evolving field of genetic vaccines based on vector DNA and recombinant, attenuated viral and bacterial vectors.


Asunto(s)
Vacunas contra el SIDAS/inmunología , Animales , Chlorocebus aethiops , Humanos , Inmunización Pasiva , Macaca mulatta , Linfocitos T Citotóxicos/inmunología , Vacunas de ADN/inmunología , Vacunas de Productos Inactivados/inmunología , Vacunas Sintéticas/inmunología
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