Conditions for Collection of Placental Tissue Samples for Culturing of Multipotent Mesenchymal Stromal Cells.

Placentas from women aged 25-32 years with normal course of gestation were studied. It is essential to stick to certain methodological approaches for preparing viable multipotent mesenchymal stromal cell culture and to carry out morphological (macro and micro) evaluation of the chorionic villi, umbilical cords, and placentas. At stage I of the study, patients' histories, labor course, and examinations of the newborns should be analyzed to exclude women with genital and extragenital diseases. At stage II, it is essential to stick to special regulations and methods for collection of specimens of the cord, amnion, and placental tissue proper. Histological control of the placental structures collected for multipotent mesenchymal stromal cell culturing is obligatory.

Ultrastructural Characteristics of Placental Telocytes.

Telocytes of placental villi were studied by electronic microscopy during physiological pregnancy. Ultrastructural features of telocytes indicating their heterogeneity and presence of at least three types of villi depending on their localization and kind were observed. All placental telocytes were characterized by small amount of organelles including mitochondria. Presence of long thin processes, which generated a branching network by contacting with each other, served as a typical feature of telocytes including telocytes of the stroma and intermediate villi. Telocytes were absent in the terminal villi.

Characteristics of Lung Injury in Rats after Inhalation of Ultradisperse Bacterial LPS in Lipophilic and Hydrophilic Phases.

We compared cellular and tissue reactions of the lungs in Wistar rats after inhalation of LPS from E. coli 0128: B12 in the form of ultradispersion in water-salt phase and lipid phase. The estimated dose of the toxin was 200 mg/kg in both series. The content of LPS and TNF-α in lung homogenates, bronchoalveolar fluid, and blood serum was determined 3, 8, and 24 h after inhalation, morphological changes in the lungs were analyzed. Inhalation of LPS in the lipid phase was accompanied by its less intensive accumulation in the lung tissue and bloodstream, and more pronounced accumulation in the bronchoalveolar fluid as well as less intense release of TNF-α in comparison with the effects of LPS in water-salt phase. Macrophage reaction, leukocyte infiltration of the interalveolar septa, and reduction of α-antitrypsin in the lung tissue was more pronounced in series with inhalation with LPS ultradispersions in water-salt phase and lipid phase, and damage to the alveolar epithelium, in the series with the inhalation of the lipid phase.

[MicroRNAs As An Important Precursors of Diagnostic Obstetric Pathology].

MicroRNAs (miRs) are the class of short nucleotide sequences (21-27 nucleotides) RNA, non-coding protein synthesis. miRs are known as effective posttranscriptional negative regulators of gene expression with specific binding sites of targeted messenger RNA (mRNA) in the cytoplasm, providing translational repression or degradation of the target miR transcript. In this review we studied the role of miRNAs in the development of a physiological pregnancy and obstetric complications. The placenta is a unique organ which provides modulation of the immune system of the maternal organism during pregnancy including miRs which determine immunological tolerance of the body to the tissues of the fetus. Thus the "placental" miRs in maternal circulation may be the potential biomarker revealed at various obstetric pathology on the early stages before clinical manifestation of the diseases.

Comparative characterization of the skull bones after implantation of titanium biocomposites containing BMP-2 in their coating structure.

The skull bone tissue was studied under conditions of implantation of Grey nanostructured titanium containing biocomposites and bone morphogenetic protein-2 in the coating structure. Implantation of sandblast processed titanium led to more rapid formation of the bone tissue than the use of titanium biocomposites with microarch oxidation (due to porous surface). The type of metal processing was inessential for nanocomposites. Morphogenetic proteins stimulated regeneration and facilitated the formation of structures characteristic of the bone tissue.

Role of innate immunity in pregnant patients with vulvovaginal infections in the development of intrauterine infection in the newborn.

A total of 115 pregnant women were examined: 59 patients with opportunistic vulvovaginal infections and 56 without infection. Congenital immunity parameters (TLR-2, NF-κB, and IL-2 receptors in placental tissue) were studied by immunohistochemical methods. Realization of congenital infection was associated with activation of IL-6 receptors and TLR-2 in the placenta and an increase of NF-κB level. These changes seemed to reflect the strained status of congenital immunity and were responsible for the intensity of local inflammatory response.

Caspase expression in placental terminal villi in spontaneous and induced pregnancy.

Placental levels of caspase 1, 8, and 10 were studied by the immunohistochemical method in full-term spontaneous and induced pregnancies. The levels of these caspases in the placental terminal villi were significantly higher in induced pregnancy. The changes were more marked after intracytoplasmatic injection of spermatozoa.

Expression of insulin-like growth factors in the placenta in preeclampsia.

Comparative morphological study of the placentas in women with preeclampsia and small-for-date fetuses was carried out. Expression of insulin-like growth factor-1 (IGF-1), insulin-like growth factor-2 (IGF-2), and insulin-like growth factor binding protein-3 (IGFBP-3) was detected by immunohistochemical methods. Low expression of IGF-1 and high expression of IGF-2 and IGFBP-3 in the placental tissue depending on preeclampsia severity were detected. The most pronounced changes were found in preeclampsia associated with small-for-date fetuses.

Morphometric evaluation of vascularization of hepatic focal nodular hyperplasia.

Comparative morphometric evaluation of the quantitative characteristics of vessels in hepatic focal nodular hyperplasia tissue was carried out on preparations stained with hematoxylin and eosin and by immunohistochemical detection of CD34 and CD105. Analysis of histological preparations stained with hematoxylin and eosin allowed the most ample characterization. Immunohistochemical detection of CD34 promoted better visualization of endotheliocytes, but just some sinusoids in the preparations were labeled. Expression of CD105 visualized the active endothelial cells and hence, helped to evaluate neoangiogenesis.

Vascular endothelial growth factor and its receptors in the placental villi of pregnant patients with pre-eclampsia.

Comparative morphological study of the placentas from women with pre-eclampsia of different severity was carried out. The expression of vascular endothelial growth factor and its receptors (VEGFR-1, VEGFR-2, VEGFR-3) was studied by immunohistochemical methods. Branched angiogenesis processes predominated in the placentas of patients with pre-eclampsia. The syncytiocapillary membranes were thickened, the number of syncytial buds was greater than normally. Immunohistochemical studies showed high expression of VEGF and VEGFR-1 and low expression of VEGFR-2 in the placental villous structures.

Morphological characteristics of the terminal villi in placental failure.

A comparative morphological study of the placentas in spontaneous and induced pregnancy aggravated by placental failure was carried out. Placental failure manifested by shrinkage of the terminal villi, lesser number and section areas of their capillaries, and higher expression of vascular endothelium growth factor and endothelial NO synthase in the syncytiotrophoblast and villous endotheliocytes. The changes were more pronounced in pregnancy developing after in vitro fertilization.

The expression of matrix metalloproteinases in placental tissue depends on the severity of undifferentiated connective tissue dysplasia.

Immunohistochemical study of MMP-2 and MMP-9 expression in placental tissue of pregnant patients with undifferentiated connective tissue dysplasia of different severity showed that more severe condition was associated with higher expression of these MMP, this underlying the development of pregnancy and labor complications. The most pronounced elevation of the studied MMP levels was found in the basal plate decidual cells in women with undifferentiated connective tissue dysplasia of more than 18 score.

Vascularization of hepatocellular carcinoma tissue depends on its differentiation degree.

Quantitative characteristics of vessels in hepatocellular carcinoma and adjacent liver tissue were studied by morphometric methods after immunohistochemical staining for CD34 and CD105. The number of immunopositive vessels decreased with reduction of tumor histological differentiation degree.

Morphofunctional changes in the kidneys in chronic endotoxemia against the background of hypothyroidism.

We studied morphological structure of the kidneys in chronic endotoxemia modeled against the background of hypothyroidism. The detected changes did not differ from those under conditions of basic endotoxemia model and were characterized by the development of glomerulosclerosis and glomerulohyalinosis. The changes were less severe in rats with hypothyroidism and appeared at the late terms of the experiment. This indicated a moderate protective effect of preexisting thyroid hormone deficiency.

Expression of antiangiogenic factors in the placental structures in pre-eclampsia.

Comparative morphological study of the placentas was carried out in women with pregnancy aggravated by the development of pre-eclampsia. Immunohistochemical methods showed increased expression of endoglin (CD105) and VEGFR1 in all placental structures. The intensity of endoglin and VEGFR1 expression was maximum in the syncytiotrophoblast and extravillous cytotrophoblast cells in severe pre-eclampsia.

Immunohistochemical characteristics of desmoid tumors.

A comparative morphological study of primary and relapsing desmoid tumors was carried out. Immunohistochemical analysis showed that the progress of desmoid tumors in the form of relapses was paralleled by an increase in the counts of immunopositive cells and intensity of ß-catenin and cycloxygenase-2 expression.

Morphometric characteristics of terminal villi of the placenta in pre-eclampsia.

Comparative morphological study of the placentas from women suffering from pre-eclampsia was carried out. Morphometric studies of histological preparations showed shrinkage and low vascularization of the placental terminal villi determining the development of hypoxia.

Morphology of endocrine organs in chronic endogenous intoxication.

Experimental study of the effects of endogenous toxic compounds on endocrine organs showed that the pathomorphological changes were caused by the cytopathic effects of endogenous toxic compounds involving the development of stereotypical reactions to injury. The severity of these reactions depended on the initial functional status of the gland and its involvement in systemic mechanisms of adaptation to the toxic process.

Nuclear ploidy is an indicator of malignancy endocrine pancreatic tumor.

Comparative morphometric and densitometric studies of cell nuclei in endocrine tumors of various differentiation degree and endocrinocytes of Langerhans islet in the adjacent pancreatic tissue were carried out. Index of proliferative activity, aneuploidy coefficient, and histogram of nuclear DNA content are recommended as additional criteria for the diagnosis of endocrine tumor malignancy.

Nuclear ploidy as an indicator of malignancy of intraductal pancreatic papillary mucinous tumors.

We carried out comparative morphometric and densitometric analysis of cell nuclei in pancreatic intraductal papillary mucinous tumors. Proliferative activity values, aneuploidy coefficient, and histogram of nuclear DNA content are recommended as additional criteria for the diagnosis of tumor malignancy.