Influence of the gut microbiota on blood acute-phase proteins.

Little is known about the bovine intestinal microbiota influence on systemic innate immune responses. The objective of the present study was to determine relationships between acute-phase proteins in blood serum of cows [C-reactive protein (CRP), LPS-binding protein (LBP) and haptoglobin (Hp)] and the faecal microbiota. Fifty-two healthy cows (2-8 years old) were investigated. Faecal bacteria were determent characterized by in situ hybridization with 16S/23S rRNA-targeted probes and by conventional culture methods. The population of Gram-negative faecal bacteria (Enterobacteriaceae) was correlated negatively with CRP and positively with LBP in blood plasma, independent of the method used. Similar results were observed with Clostridium perfringens. No correlation was found between the faecal population of intestinal bacteria and Hp levels in blood plasma. This datum indicates that intestinal bacteria, especially Enterobacteriaceae and C. perfringens, may influence the level of CRP and LBP in blood plasma. These findings can be very important for diagnostic evaluations of the intestinal microbiota and provide specific information about its regulation.

Effect of a Potential Probiotic Candidate Enterococcus faecalis-1 on Growth Performance, Intestinal Microbiota, and Immune Response of Commercial Broiler Chickens.

The probiotic effect of Enterococcus faecalis-1 (isolated from healthy chickens) on growth performance, immune response, and modulation of the intestinal microbiota of broilers was assessed with a total of 100-day-old commercial Cobb chicks. The chicks were randomly divided into two equal groups. The control group received a basal diet, while the test group received a basal diet and was orally supplied with E. faecalis at a dose of 108 CFU/bird/day. Results showed that E. faecalis-1 supplement significantly (P < 0.05) improved the body weight and feed conversion ratio of treated broilers compared with the control ones. The mortality percentage was reduced in E. faecalis-1-supplemented group. The total IgY serum level was significantly (P < 0.05) increased in broilers receiving E. faecalis-1 supplement (7.1 ± 0.39) compared with the control group (5.8 ± 0.3), while the serum avidin level was significantly (P < 0.05) decreased in E. faecalis-1-supplemented broilers (76 ± 11.1). There was no significant change in the immune response towards avian influenza and Newcastle vaccines in both groups. The total Lactobacillus and Enterococcus counts were significantly (P < 0.05) higher in the cecal contents of broilers given E. faecalis-1 than those that received the control treatment. E. faecalis-1 supplement enhanced the enzyme activities, antioxidant system, and liver functions of treated broilers compared with those in the control group. Collectively, these results showed that E. faecalis-1 could promote growth performance and immunological status and convey beneficial modulation of the cecal microbiota in broilers.

Necrotic enteritis in chickens: development of a straightforward disease model system.

The interaction between Eimeria species and Clostridium perfringens was investigated in two different necrotic enteritis (NE) models: 120-day-old broilers were used in two separate experiments consisting of six groups (n=10) each. Besides controls, chickens were infected with coccidia on study day (SD) 18 (Eimeria maxima and Eimeria acervulina (experiment 1) or Eimeria tenella and Eimeria brunetti (experiment 2) and/or a NetB toxin positive C perfringens strain (both experiments: SD 14 or SD 22, respectively)). Body weight, feed intake, mortality rate, clinical disease, Eimeria species oocyst excretion and C perfringens counts were recorded. NE and coccidiosis specific lesion scores were assessed (SD 24 and SD 30). In coinfected groups, NE-typical clinical signs occurred. Coccidiosis-specific lesions were most severe in coinfected groups (significant for E tenella, P<0.05). Most pronounced NE lesions occurred in coinfected chickens compared with C perfringens monoinfected groups (experiment 2, C perfringens infections on SD 22: P<0.05). In experiment 2, E tenella antibody levels were (non-significantly) higher in coinfected groups than in Eimeria species monoinfected groups. Thus, infection with E tenella and Eimeria brunetti followed by C perfringens inoculation is regarded as an easy to handle and suitable model for investigations into NE of chickens.

Development of a recombinant ELISA using yeast (Pichia pastoris)-expressed polypeptides for detection of antibodies against avian influenza A subtype H5.

Two truncated sequences (designated P1 and rHA1) of influenza A virus subtype H5 haemagglutinin (HA) were cloned and expressed in yeast Pichia pastoris (P. pastoris). These polypeptides were used in an indirect recombinant ELISA (rELISA) for detection of H5 antibodies in poultry. Serum samples obtained from broiler chickens vaccinated with commercial inactivated vaccine (H5N2) and control negative sera from non-vaccinated chickens against influenza were tested using rP1-ELISA, rHA1-ELISA, whole H5N1-ELISA, Western blot, agar gel immunodiffusion (AGID) and haemagglutination inhibition (HI) tests. The rHA1-ELISA proved to be highly sensitive and specific. To study the validity of rHA1-ELISA, a total of 179 serum samples obtained from commercial broiler chickens vaccinated previously with commercial H5N2 inactivated vaccines, were tested by rHA1-ELISA, commercial ELISA (cELISA) and HI. The relative sensitivity and specificity between rHA1-ELISA, and HI tests were 100% and 70%, respectively, and between cELISA and HI were 100% and 57%, respectively. The agreement ratio between rHA1-ELISA and HI was 84.9% and between cELISA and HI tests was 76.5%. Serum samples obtained from ducks vaccinated with commercial inactivated H5N2 were tested by rHA1-ELISA and the results showed significant reactivity with duck sera. In conclusion, the results demonstrate the potential applicability of the rELISA for the determination of antibodies to H5 influenza virus in chickens and ducks.

Chemical and amino acid composition of Iraqi mung beans.

The chemical composition of five samples of Iraqi mung beans chosen from different geographical regions were investigated as well as their amino acid contents. The results indicated that all the samples were good sources of protein, carbohydrates and minerals. They contained 24.95-28.04% protein, 64,15-66.32% carbohydrates, 0.86-0.96% crude fat, 3.37-4.05% ash and 4.13-5.01% crude fibre. The amounts of phosphorus, calcium and iron were within the ranges; 381-528, 128-143 and 5.14-5.76 milligrams per 100 grams of flour, respectively (all results on a dry weight basis). Further, all samples were rich in most essential amino acids, especially lysine (5.85-8.24 grams per 100 grams of protein) but they were deficient in methionine (0.96-1.48 gram per 100 grams of protein).

Selected nutritional, physical and sensory characteristics of pan and flat breads prepared from composite flours containing fababean.

Composite flour blends containing wheat (W), fababean (F), cottonseed and sesame flours were formulated to provide the FAO/WHO/UNU protein requirements for the 2-5 year old child, and evaluated in pan and flat bread applications. Water absorption of composite flour doughs was up to 35% greater than the control but gluten strength and slurry viscosities were markedly reduced. Loaf volume and specific volume of pan breads prepared from composite flours were 25-60% less than that of the control bread but flat breads tolerated the protein supplements extremely well. The W/F flat bread, containing 27% of fababean flour, received acceptable taste, texture and colour scores and was only slightly inferior to the control in puffing and layer separation. Additions of cottonseed or sesame flours to the W/F blend failed to improve sensory properties of the flat breads.