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1.
Korean J Parasitol ; 57(3): 319-324, 2019 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-31284358

RESUMEN

This study aimed to investigate the tick species and give background for tick-borne investigations in Korea. Ticks were collected from the area within 2 km radius of the 4 domestic animal farms, where they were located in mountainous areas and raising animals on pasture, and from animal bodies in 2014 and 2015. In total, 7,973 nymphal and adult ticks were collected from the farms - 7,758 Haemaphysalis longicornis, 198 Haemaphysalis flava, and 17 Ixodes nipponensis, and 1,763 were collected from animals - 729 H. longicornis from cattle; 569 H. longicornis from goats; and 297 H. longicornis, 118 H. flava, 1 I. nipponensis, and 49 Amblyomma testudinarium from wild boars. As more species of ticks were collected from wild boars than domesticated animals and their habitats, various animal hosts should be considered while investigating tick species.


Asunto(s)
Bovinos/parasitología , Cabras/parasitología , Ixodidae/clasificación , Sus scrofa/parasitología , Infestaciones por Garrapatas/veterinaria , Animales , Animales Domésticos/parasitología , Femenino , Ixodidae/genética , Ixodidae/fisiología , Masculino , República de Corea/epidemiología , Infestaciones por Garrapatas/epidemiología , Infestaciones por Garrapatas/parasitología
2.
Korean J Parasitol ; 55(2): 185-191, 2017 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-28506041

RESUMEN

Tick is one of the most important arthropods in the transmission of vector-borne diseases. In this study, we investigated the abundance and species of ticks associated with swine and their habitats to assess the risk of spread of tick-borne diseases in host species, such as wild boars. Ticks were collected from 24 grazing or traditionally reared domestic pig farms and 8 habitats of wild boars in 8 provinces and 1 city in the Republic of Korea, by using the dragging and flagging methods. Ticks were also collected directly from 49 wild boars by using fine forceps. A total of 9,846 hard ticks were collected, including 4,977 Haemaphysalis longicornis, 4,313 Haemaphysalis flava, 508 Ixodes nipponensis, 1 Ixodes turdus, and 47 Amblyomma testudinarium. A total of 240 hard ticks were collected from 49 wild boars, including 109 H. flava, 84 H. longicornis, and 47 A. testudinarium. A total of 578 hard ticks were collected from areas around domestic pig farms. Only 2 hard tick species, 546 H. longicornis and 32 H. flava, were collected from these areas. A total of 9,028 hard ticks were collected from wild boars of 8 habitats, including 4,347 H. longicornis, 4,172 H. flava, 508 I. nipponensis, and 1 I. turdus. A. testudinarium was collected only from wild boars, and I. nipponensis and I. turdus were collected only from the habitats of wild boars.


Asunto(s)
Animales Domésticos/parasitología , Animales Salvajes/parasitología , Ecosistema , Sus scrofa/parasitología , Infestaciones por Garrapatas/parasitología , Infestaciones por Garrapatas/veterinaria , Garrapatas , Animales , República de Corea/epidemiología , Infestaciones por Garrapatas/epidemiología , Enfermedades por Picaduras de Garrapatas/parasitología , Enfermedades por Picaduras de Garrapatas/prevención & control , Enfermedades por Picaduras de Garrapatas/transmisión , Garrapatas/clasificación , Garrapatas/patogenicidad
3.
Jpn J Vet Res ; 64(2): 123-9, 2016 May.
Artículo en Inglés | MEDLINE | ID: mdl-27506086

RESUMEN

Canine atopic dermatitis (CAD) is a chronic relapsing inflammatory skin disease occurring in 10% of the canine population. Although most studies have focused on the pathophysiological mechanism involved in CAD, the detrimental impact of CAD on quality of life has received only little attention. Hair cortisol analysis is becoming a valuable tool in monitoring chronic stress. To further validate this approach in CAD, we compared the hair cortisol concentration of atopic dogs with that of healthy conditioned dogs. The extent and severity of cutaneous lesions of atopic dermatitis were assessed according to modified CADESI-03 scores. In addition, skin barrier function was evaluated by measuring transepidermal water loss (TEWL) and stratum corneum conductance. The correlation between CAD severity and hair cortisol concentration was evaluated. The level of hair cortisol evaluated by ELISA assay showed that the atopic dermatitis group had significantly increased cortisol levels compared to that of the healthy control group. A significant positive correlation was identified between hair cortisol level and the CADESI score in CAD patients. The TEWL value of the cubital flexor of the forelimb in the atopic group was significantly higher compared to the healthy controls. These findings imply that the hair cortisol analysis can be an effective and objective biomarker in assessment of long-term stress of CAD patients.


Asunto(s)
Dermatitis Atópica/veterinaria , Enfermedades de los Perros/metabolismo , Cabello/química , Hidrocortisona/metabolismo , Animales , Dermatitis Atópica/metabolismo , Perros , Femenino , Hidrocortisona/química , Masculino
4.
Can Vet J ; 54(9): 869-72, 2013 Sep.
Artículo en Inglés | MEDLINE | ID: mdl-24155492

RESUMEN

A 50-day-old, female miniature schnauzer dog was presented for astasia, dorsoventral flattening of the thorax, hypoplasia of hind-limb muscles, stiffness of hind-limb joints, paddling leg motion, and panting. The dog was diagnosed with swimmers syndrome. The dog recovered completely following 40 days of home-care treatment that involved environmental and nutritional management along with intensive physiotherapy.


Traitement à domicile du syndrome du chiot nageur chez un Schnauzer miniature. Une chienne Schnauzer miniature âgée de 50 jours est présentée pour de l'astasie, un aplatissement dorsiventral du thorax, l'hypoplasie des muscles des pattes arrières, une rigidité des articulations des pattes arrières, un mouvement de pédalage des pattes et des halètements. La chienne a été diagnostiquée avec le syndrome du chiot nageur. La chienne s'est complètement rétablie après 40 jours de traitements à domicile qui ont inclus une gestion de l'environnement et de la nutrition avec une physiothérapie intensive.(Traduit par Isabelle Vallières).


Asunto(s)
Enfermedades de los Perros/congénito , Modalidades de Fisioterapia/veterinaria , Crianza de Animales Domésticos , Animales , Enfermedades de los Perros/terapia , Perros , Femenino
5.
J Zoo Wildl Med ; 43(1): 174-6, 2012 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-22448528

RESUMEN

Rabies has been reported in dogs, cattle, and raccoon dogs in Korea, especially in the provinces of Gyeonggi and Gangwon. The aim of this study was to investigate the seroprevalence of the rabies in wild Korean raccoon dogs by using 50 blood samples collected in Gyeonggi and Gangwon provinces. Antibodies against rabies were measured by using a fluorescent antibody virus neutralization test. Of the 50 blood samples tested, 20 raccoon dogs (40%) tested positive. Seropositive animals were distributed in Paju, Yangpyeong, Goyang, Yangju, Yanggu, and Hoengseong cities.


Asunto(s)
Anticuerpos Antivirales/sangre , Vacunas Antirrábicas/inmunología , Virus de la Rabia/inmunología , Rabia/veterinaria , Perros Mapache , Animales , Rabia/sangre , Rabia/epidemiología , Rabia/prevención & control , Vacunas Antirrábicas/administración & dosificación , República de Corea/epidemiología
6.
Ticks Tick Borne Dis ; 12(4): 101689, 2021 07.
Artículo en Inglés | MEDLINE | ID: mdl-33676201

RESUMEN

Ticks are considered important vectors among arthropods and are linked to serious medical and veterinary health problems. In this study, we investigated tick-borne pathogens (TBPs) of Ornithodoros (Carios) sawaii and a newly identified Ornithodoros species from migratory bird nests in the uninhabited islands of the Republic of Korea (ROK). Ticks were collected from seabird nests with soil using a Tullgren funnel. Polymerase chain reaction (PCR) was performed using specific primer sets targeting genes of Borrelia spp., Rickettsia sp., Anaplasma phagocytophilum, Anaplasma bovis, and Bartonella spp. for molecular identification of TBPs, and two pathogens, Borrelia sp. and Rickettsia sp. were detected via PCR. Sequence data were analyzed and a phylogenetic analysis was conducted using the maximum-likelihood method in MEGA v.7. The detection rate of Borrelia sp. in O.(C.) sawaii was 6.8 % (5/74), and that of Rickettsia sp. in O. sawaii and the newly identified Ornithodoros species. was 36.5 % (27/74). Sequencing analysis revealed that the 16S ribosomal (r) RNA and flagellin genes of Borrelia sp., and the citrate synthase (gltA) and 17-kDa antigen gene of Rickettsia sp. were closely phylogenetically related to those of Borrelia turicatae and Rickettsia asembonensis. This is the first report identifying Borrelia sp. and Rickettsia sp. from O. sawaii, and Rickettsia sp. from the newly identified Ornithodoros species in the ROK, and these results imply that soft ticks (O. sawaii, and the newly identified Ornithodoros species) may function as pathogen carriers with important implications for public health throughout their distribution areas in Asia.


Asunto(s)
Borrelia/aislamiento & purificación , Ornithodoros/microbiología , Rickettsia/aislamiento & purificación , Animales , Femenino , Masculino , Ninfa/crecimiento & desarrollo , Ninfa/microbiología , Ornithodoros/crecimiento & desarrollo , República de Corea , Especificidad de la Especie
7.
J Vet Med Sci ; 82(11): 1685-1692, 2020 Dec 05.
Artículo en Inglés | MEDLINE | ID: mdl-33028748

RESUMEN

The field distribution of the oral rabies vaccine is effective in controlling the spread of rabies. The present study aimed to investigate efficient distribution locations based on the environment, contact rate, and consumption by target wildlife species in South Korea. The target species (Korean raccoon dogs, domestic dogs, and feral cats) accounted for 945 contacts (52.2%), in total 1,808 contacts. There were 863 (47.8%) contacts by non-target species. Raccoon dogs, a main reservoir of rabies in South Korea, had the highest contact rate (34.1%) among all species. The contact rate by target species was highest at riparian sites and bushy mountainous vegetation, where raccoon dogs are abundant. There was remarkable contact by raccoon dogs in mountainous areas below 150 m with bushy vegetation. Our results indicate that these locations are efficient areas for vaccine distribution, especially targeting the raccoon dog. Vaccines were continuously contacted with intervals ranging from one hour to one day. Vaccines at 94.4% of the distribution points were completely consumed within two weeks. The mean consumption rate was 95.2 ± 1.93% during the overall study period. These findings suggest that the oral rabies vaccine attracts wildlife including domestic dogs and feral cats. Our results suggest that low sections of mountainous areas with bushy vegetation and/or neighboring riparian areas are rich in target wildlife species (especially raccoon dogs) and are efficient locations for vaccine distribution to control rabies in South Korea.


Asunto(s)
Enfermedades de los Gatos , Vacunas Antirrábicas , Virus de la Rabia , Rabia , Animales , Animales Salvajes , Gatos , Rabia/epidemiología , Rabia/prevención & control , Rabia/veterinaria , Perros Mapache , República de Corea/epidemiología
8.
J Parasitol ; 106(5): 546-563, 2020 10 01.
Artículo en Inglés | MEDLINE | ID: mdl-32916707

RESUMEN

Ticks and tick-borne diseases are important issues worldwide because of their effects on animal and human health. The genus Ornithodoros, which is included in the family Argasidae, is typically associated with wild animals, including seabirds. In this study, samples from the nests of seabirds and surrounding soil were collected to investigate Ornithodoros spp. from 9 uninhabited islands in the western, eastern, and southern parts of Korea from April 2017 to October 2018. The islands are known as the breeding places of migratory and resident birds. Ticks were collected from soil and nest material of seabirds using a Tullgren funnel and identified using 16S rRNA and the cytochrome c oxidase 1 gene (COI), and host animals of soft ticks were identified using the mitochondrial DNA cytochrome b gene by a polymerase chain reaction. In the sequence identity of the 16S rRNA gene fragment of Ornithodoros sp., Ornithodoros sawaii was identified as the closest homologous sequence, and the new Ornithodoros sp. was newly identified. We found that the newly identified Ornithodoros sp. in the Republic of Korea was located in uninhabited islands used as breeding places by the black-tailed gull, Larus crassirostris.


Asunto(s)
Enfermedades de las Aves/parasitología , Ornithodoros/clasificación , Infestaciones por Garrapatas/veterinaria , Animales , Aves , Clonación Molecular , ADN/química , ADN/aislamiento & purificación , Complejo IV de Transporte de Electrones/genética , Islas , Funciones de Verosimilitud , Microscopía Electrónica de Rastreo , Ornithodoros/genética , Ornithodoros/ultraestructura , Filogenia , Reacción en Cadena de la Polimerasa , ARN Ribosómico 16S/genética , República de Corea , Suelo/parasitología , Infestaciones por Garrapatas/parasitología
9.
J Vet Sci ; 10(1): 85-7, 2009 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-19255530

RESUMEN

Blood, saliva, and nail samples were collected from 54 dogs and 151 cats and analyzed for the presence of Bartonella henselae with a novel nested polymerase chain reaction (PCR) method. Bartonella (B.) henselae was detected in feral cat blood (41.8%), saliva (44.1%), and nail (42.7%) samples. B. henselae was also detected in pet cat blood (33.3%), saliva (43.5%), and nail (29.5%) samples and in pet dog blood (16.6%), saliva (18.5%), and nail (29.6%) samples. Nine samples were infected with B. clarridgeiae and 2 were co-infected with B. henselae and B. clarridgeiae of blood samples of dogs. This report is the first to investigate the prevalence of B. henselae and B. clarridgeiae in dogs and cats in Korea, and suggests that dogs and cats may serve as potential Bartonella reservoirs.


Asunto(s)
Infecciones por Bartonella/veterinaria , Bartonella/clasificación , Enfermedades de los Gatos/microbiología , Enfermedades de los Perros/microbiología , Animales , Infecciones por Bartonella/sangre , Infecciones por Bartonella/epidemiología , Infecciones por Bartonella/microbiología , Enfermedades de los Gatos/sangre , Enfermedades de los Gatos/epidemiología , Gatos , Reservorios de Enfermedades/veterinaria , Enfermedades de los Perros/epidemiología , Perros , Pezuñas y Garras/microbiología , Corea (Geográfico)/epidemiología , Prevalencia , Saliva/microbiología
10.
J Zoo Wildl Med ; 39(4): 671-3, 2008 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-19110717

RESUMEN

Infestation with Sarcoptes scabiei was diagnosed from four wild raccoon dogs (Nyctereutes procyonoides) accidentally captured and presented to the Animal Health Center in Seoul Grand Park Zoo, Korea. Diagnosis was done by microscopic and histologic examination from skin lesions. Sarcoptes scabiei was the only species detected from the lesions and characterized by dorsoventrally flattened and round bodies, sucker-like pulvilli borne on long nonjointed pretarsi, triangular scales and spinelike setae on the dorsum, and three epimeres that are chitinous extensions of the coxae of the legs. In addition, infiltration of mast cells in the dermis was associated with infestation of the burrowing mite. This is the first report of sarcoptic mange in raccoon dogs in Korea. Because heavy infestation with S. scabiei was found in all of the captured wild raccoon dogs, further work is necessary to develop prophylactic interventions to prevent the spread of sarcoptic mange in free-living raccoon dogs in Korea.


Asunto(s)
Perros Mapache , Sarcoptes scabiei/crecimiento & desarrollo , Escabiosis/veterinaria , Animales , Animales Salvajes/parasitología , Femenino , Corea (Geográfico)/epidemiología , Masculino , Perros Mapache/parasitología , Sarcoptes scabiei/patogenicidad , Escabiosis/epidemiología , Escabiosis/patología , Índice de Severidad de la Enfermedad
11.
J Zoo Wildl Med ; 39(1): 118-20, 2008 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-18432107

RESUMEN

A 10-yr-old male South American sea lion (Otaria byronia) died after several weeks of depression, anorexia, weight loss, and progressive respiratory distress. At necropsy, three confluent, lobulated, dark-red masses were noted in the mesentery. Similar masses were also observed in the lung and both kidneys. Hemangiosarcoma was diagnosed based on gross findings, histopathology, and immunohistochemistry. This is the first case of hemangiosarcoma reported in pinnipeds.


Asunto(s)
Hemangiosarcoma/veterinaria , Neoplasias Renales/veterinaria , Neoplasias Pulmonares/veterinaria , Leones Marinos , Animales , Resultado Fatal , Hemangiosarcoma/diagnóstico , Hemangiosarcoma/patología , Inmunohistoquímica/veterinaria , Neoplasias Renales/diagnóstico , Neoplasias Renales/patología , Neoplasias Pulmonares/diagnóstico , Neoplasias Pulmonares/patología , Masculino
12.
Am J Trop Med Hyg ; 98(4): 1061-1068, 2018 04.
Artículo en Inglés | MEDLINE | ID: mdl-29436346

RESUMEN

Anaplasmosis, cat-scratch disease, and Lyme disease are emerging vector-borne infectious diseases in Korea. Although the prevalence of vector-borne pathogens (VBPs) in domestic animals and vector arthropods has been documented, there is limited information on the presence of VBPs in wild animals. The raccoon dog (Nyctereutes procyonoides), a wild canid found in East Asia and Europe, represents a potential wildlife reservoir for zoonotic diseases. To investigate the prevalence of VBPs in raccoon dogs, 142 carcasses and 51 blood samples from captured raccoon dogs were collected from 2003 to 2010 and from 2008 to 2009, respectively, in Korea. In addition, 105 Haemaphysalis flava (14 larvae, 43 nymphs, 32 males, and 16 females) and nine Haemaphysalis longicornis (all female) were collected from three raccoon dogs. Samples of the spleen and blood were tested for the presence of VBPs by using nested polymerase chain reaction. Among the samples collected from 193 raccoon dogs and 114 ticks, two samples were positive for Anaplasma phagocytophilum, four for Anaplasma bovis, two for Borrelia theileri, and two for Bartonella henselae. To the best of our knowledge, this study is the largest survey of raccoon dogs aimed at the analysis of VBPs in this species. Moreover, the present study represents the first identification of A. phagocytophilum, B. henselae, and B. theileri in raccoon dogs in their native habitat (East Asia).


Asunto(s)
Anaplasma/aislamiento & purificación , Bartonella/aislamiento & purificación , Borrelia/aislamiento & purificación , Perros Mapache/microbiología , Anaplasma/genética , Animales , Bartonella/genética , Borrelia/genética , Femenino , Masculino , Reacción en Cadena de la Polimerasa
13.
Cloning Stem Cells ; 9(1): 130-7, 2007.
Artículo en Inglés | MEDLINE | ID: mdl-17386020

RESUMEN

Over the world, canine species, including the gray wolf, have been gradually endangered or extinct. Many efforts have been made to recover and conserve these canids. The aim of this study was to produce the endangered gray wolf with somatic cell nuclear transfer (SCNT) for conservation. Adult ear fibroblasts from a female gray wolf (Canis lupus) were isolated and cultured in vitro as donor cells. Because of limitations in obtaining gray wolf matured oocytes, in vivo matured canine oocytes obtained by flushing the oviducts from the isthmus to the infundibulum were used. After removing the cumulus cells, the oocyte was enucleated, microinjected, fused with a donor cell, and activated. The reconstructed cloned wolf embryos were transferred into the oviducts of the naturally synchronized surrogate mothers. Two pregnancies were detected by ultrasonography at 23 days of gestation in recipient dogs. In each surrogate dog, two fetal sacs were confirmed by early pregnancy diagnosis at 23 days, but only two cloned wolves were delivered. The first cloned wolf was delivered by cesarean section on October 18, 2005, 60 days after embryo transfer. The second cloned wolf was delivered on October 26, 2005, at 61 days postembryo transfer. Microsatellite analysis was performed with genomic DNA from the donor wolf, the two cloned wolves, and the two surrogate female recipients to confirm the genetic identity of the cloned wolves. Analysis of 19 microsatellite loci confirmed that the cloned wolves were genetically identical to the donor wolf. In conclusion, we demonstrated live birth of two cloned gray wolves by nuclear transfer of wolf somatic cells into enucleated canine oocyte, indicating that SCNT is a practical approach for conserving endangered canids.


Asunto(s)
Clonación de Organismos , Fibroblastos , Técnicas de Transferencia Nuclear , Oocitos , Lobos , Animales , Perros , Extinción Biológica , Femenino , Fibroblastos/citología , Genoma/genética , Masculino , Repeticiones de Microsatélite/genética , Embarazo , Lobos/genética
14.
J Vet Med Sci ; 69(8): 851-2, 2007 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-17827895

RESUMEN

Pyelonephritis, in which Staphylococcus intermedius was isolated, was diagnosed in a 4-year-old female Siberian tiger (Panthera tigris altaica). At necropsy, the renal pelvis was dilated with a large amount of purulent exudates. Microscopically, the lesions of renal pelvis and medulla consisted of necrotic foci intermingled primarily with numerous degenerative neutrophils and a few lymphocytes, plasma cells and macrophages. Bacteriology showed the presence of S. intermedius. This is the first report on pyelonephritis associated with S. intermedius in wild felidae.


Asunto(s)
Pielonefritis/veterinaria , Infecciones Estafilocócicas/veterinaria , Tigres/microbiología , Absceso/microbiología , Absceso/patología , Animales , Resultado Fatal , Femenino , Riñón/microbiología , Riñón/patología , Pielonefritis/microbiología , Infecciones Estafilocócicas/microbiología
15.
J Vet Sci ; 8(1): 103-5, 2007 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-17322782

RESUMEN

A 7-year-old female Eurasian otter (Lutra lutra) at the Seoul Grand Park, Korea, died after displaying depression, anorexia, weight loss and rough skin for several days. At necropsy, a solitary friable round mass, which was approximately 12 x 9 x 5 cm and mottled dark red and yellow, was found bulging from the right hepatic lobe. Microscopically, the nonencapsulated, poorly circumscribed mass was composed of solid sheets of neoplastic hepatocytes. In addition, numerous small tan foci, ranging from 0.5 to 1.0 cm in diameter, were evenly scattered throughout the pancreatic tissue. These foci were found to be nonencapsulated, well-demarcated hyperplastic nodules of the exocrine pancreatic gland. We observed neither intrahepatic nor extrahepatic metastases. Based on the gross and microscopic changes, we diagnosed the animal as having a hepatocellular adenoma accompanied by exocrine pancreatic nodular hyperplasia.


Asunto(s)
Adenoma de Células Hepáticas/patología , Adenoma de Células Hepáticas/veterinaria , Neoplasias Hepáticas/patología , Neoplasias Hepáticas/veterinaria , Nutrias , Animales , Resultado Fatal , Femenino , Corea (Geográfico) , Páncreas Exocrino/patología
16.
J Vet Med Sci ; 79(7): 1278-1283, 2017 Jul 28.
Artículo en Inglés | MEDLINE | ID: mdl-28539532

RESUMEN

Sociability is an essential trait for dogs to successfully interact with humans. In this study, the relationship between sociability and physiological stress was examined. Additionally, whether differences exist between companion dogs (C group) and shelter dogs (S group) was examined. Overall, healthy 37 dogs (C group=21 and S group=16) were examined. After 5 min of walking, the dog and the owner (or the chief manager) rested freely in the experimental location for 5 min. The behavioral test with 6 categories was conducted to evaluate sociability over 4 min. The establishment of two groups (H group=dogs with high sociability; L group=dogs with low sociability) was supported by the statistical results of the behavioral tests. Saliva was collected before (P1) and after the test period (P2), and salivary cortisol levels were determined and statistically analyzed. The cortisol concentrations at P2 and the differences in concentrations between P1 and P2 (P2-P1) in the groups with high sociability were significantly lower than those in the groups with low sociability. These results may demonstrate that sociable dogs adapt more comfortably to strangers and unfamiliar situations. Meanwhile, there were significant differences in hormonal results between the C and S groups. For this reason, their sociability should be evaluated using behavioral and physiological assessments before re-adoption to ensure their successful adaptation.


Asunto(s)
Perros/psicología , Conducta Social , Animales , Perros/fisiología , Femenino , Humanos , Hidrocortisona/análisis , Masculino , Saliva/química , Estrés Fisiológico
17.
J Vet Diagn Invest ; 18(1): 130-3, 2006 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-16566273

RESUMEN

Transmissible venereal tumor (TVT) is a well-documented transplantable tumor in dogs, with no breed or sex predilection and a low metastatic rate. In this report, a 2-year-old intact female Mastiff that had numerous, rapidly growing masses throughout the subcutis mainly at the dorsal body plane, the caudal half of the ventral abdomen, and around the vulva was euthanized due to poor prognosis. Neoplastic nodules similar to those seen in the subcutis were also noted in the lung, anterior mediastinum, liver, spleen, kidney, and superficial and deep lymph nodes in both abdominal and thoracic cavities. The neoplastic nodules from the subcutis as well as metastatic foci revealed similar cytologic and histologic features, which were consistent with canine TVT. By immunohistochemical staining, the neoplastic cells were positive for lysozyme and vimentin but were negative for cytokeratin, desmin, CD3, and CD79a. The diagnosis of the TVT was further supported by the identification and analysis of long interspersed nuclear elements (LINE) from paraffin-embedded tumor tissue. This case is a rare example of TVT with multiorgan metastasis. In this case, the polymerase chain reaction technique was useful in differential diagnosis of canine round cell tumors because this technique can be applied in retrospective as well as future study.


Asunto(s)
Enfermedades de los Perros/diagnóstico , Tumores Venéreos Veterinarios/diagnóstico , Animales , Biomarcadores de Tumor/análisis , Diagnóstico Diferencial , Enfermedades de los Perros/patología , Perros , Resultado Fatal , Femenino , Inmunohistoquímica/veterinaria , Muramidasa/análisis , Reacción en Cadena de la Polimerasa/veterinaria , Pronóstico , Tumores Venéreos Veterinarios/patología , Tumores Venéreos Veterinarios/secundario , Vimentina/análisis
18.
J Vet Med Sci ; 68(11): 1167-71, 2006 Nov.
Artículo en Inglés | MEDLINE | ID: mdl-17146173

RESUMEN

Interspecies cloning may be a useful method to help conserve endangered species and to study nuclear-cytoplasm interaction. The present study investigated in vitro development of goral (Naemorhedus goral) intergeneric nuclear transfer embryos produced by fusing goral fibroblasts with enucleated metaphase II (MII) bovine oocytes. After two to five passages, serum-starved or non-starved goral skin fibroblast cells were transferred into enucleated MII bovine oocytes. Couplets were electrically fused and chemically activated, and then cultured in either modified synthetic oviduct fluid (mSOF) or tissue culture medium-199 (TCM-199) supplemented with 10% FBS. Serum starvation of donor cells did not affect the fusion rate and or development to of cells to the two-cell stage, to more than 9-cells, or to morulae, regardless of culture medium. Three blastocysts from 202 fused embryos were obtained when embryos reconstructed with non- serum- starved donor cells were cultured in mSOF. However, no blastocysts were obtained when the embryos reconstructed with serum-starved donor cells were cultured in mSOF. The total cell number of goral intergeneric embryos averaged 130.3 (range 105-180). In conclusion, this study demonstrated that bovine oocytes can support blastocyst development after intergeneric SCNT with goral fibroblasts.


Asunto(s)
Clonación de Organismos/veterinaria , Técnicas de Transferencia Nuclear/veterinaria , Rumiantes/embriología , Animales , Blastocisto/fisiología , Clonación de Organismos/métodos , Fibroblastos/fisiología , Modelos Lineales , Oocitos/fisiología , Especificidad de la Especie
19.
J Vet Sci ; 7(4): 369-74, 2006 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-17106229

RESUMEN

Betanodaviruses are the causative agents of viral nervous necrosis (VNN) in cultured marine fish. A total of 237 apparently healthy aquarium fish, marine (65 species) and freshwater (12 species) fishes and marine invertebrates (4 species), which were stocked in a commercial aquarium in Seoul, South Korea, were collected from November 2005 to February 2006. The brains of the fish and other tissues of the invertebrates were examined by reverse transcriptase-polymerase chain reaction (RT-PCR) and nested PCR to detect betanodavirus. Positive nested PCR results were obtained from the brains of 8 marine fish species (shrimp fish Aeoliscus strigatus, milkfish Chanos chanos, three spot damsel Dascyllus trimaculatus, Japanese anchovy Engraulis japonicus, pinecone fish Monocentris japonica, blue ribbon eel Rhinomuraena quaesita, look down fish Selene vomer, yellow tang Zebrasoma flavesenes), 1 marine invertebrate species (spiny lobster Pamulirus versicolor), and 2 freshwater fish species (South American leaf fish Monocirrhus polyacanthus and red piranha Pygocentrus nattereri). The detection rate in nested PCR was 11/237 (4.64%). These subclinically infected aquarium fish and invertebrates may constitute an inoculum source of betanodaviruses for cultured fishes in the Korean Peninsula.


Asunto(s)
Enfermedades de los Peces/virología , Nodaviridae/aislamiento & purificación , Infecciones por Virus ARN/veterinaria , Animales , Crustáceos , Enfermedades de los Peces/epidemiología , Peces , Corea (Geográfico)/epidemiología , Nodaviridae/genética , Infecciones por Virus ARN/epidemiología , Infecciones por Virus ARN/virología , ARN Viral/química , ARN Viral/genética , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa/veterinaria
20.
J Vet Sci ; 17(2): 153-8, 2016 Jun 30.
Artículo en Inglés | MEDLINE | ID: mdl-26645334

RESUMEN

Separation anxiety (SA) is a serious behavioral problem in dogs. In this study, salivary cortisol was studied to determine if the owner's odor or voice could reduce SA in dogs. Twenty-eight dogs with SA were divided into three groups: group 1 (control), group 2 (with owner's clothes during the separation period; SP) and group 3 (a recording of the owner's voice was played during SP). The dog's saliva was collected after the owner and their dog were in the experimental room for 5 min (PRE). The dog was then separated from the owner for 20 min and saliva collected four times at intervals of 5 min (SP1-4). Finally, the owner was allowed back into the room to calm the dog for 5 min, after which saliva was collected (POST). Evaluation of salivary cortisol concentrations by ELISA revealed that the ratios of SP1 concentration to PRE or POST concentrations were significantly higher in group 1 than in group 2 or 3. Additionally, the concentrations of SP1-PRE and SP1-POST among groups differed significantly. These findings indicate that the owner's odor or voice may be helpful to managing stress in dogs with SA.


Asunto(s)
Estimulación Acústica/veterinaria , Ansiedad de Separación/prevención & control , Enfermedades de los Perros/prevención & control , Hidrocortisona/metabolismo , Olfatometría/veterinaria , Animales , Biomarcadores/análisis , Perros , Femenino , Masculino , Saliva/química , Estrés Fisiológico
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