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1.
Int J Syst Evol Microbiol ; 69(8): 2431-2439, 2019 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-31188092

RESUMEN

A novel actinobacterium, designated strain 1AS2cT, was isolated from the wheat rhizosphere collected from the Brazilian Cerrado biome. A polyphasic approach, including phenotypic characterization and phylogenetic multilocus sequence analysis (MLSA), was used to determine the taxonomic position of this strain. Analysis of the 16S rRNA gene sequence indicated that the novel strain is closely related to Streptomyces cavourensis NBRC 13026T, Streptomyces albolongus NBRC 13465T and Streptomyces araujoniae ASBV-1T, sharing a similarity value of 98.2, 98.1 and 97.9 %, respectively. Additionally, MLSA of five housekeeping genes (atpD, gyrB, recA, rpoB and trpB) showed evolutionary distances beyond the 0.007 threshold, as well as low DNA-DNA relatedness between strain 1AS2cT and its closest phylogenetic neighbours (S. cavourensis NBRC 13026T, S. albolongus NBRC 13465T and S. araujoniae ASBV-1T: 56, 62.5 and 63.0 %, respectively), indicating a new phylogenetic lineage. The phylogenetic, chemotaxonomic and phenotypic characteristics support the assignment of strain 1AS2cT to the genus Streptomyces, representing a novel species. It is concluded that strain 1AS2cT (=CMAA 1679T=NRRL B-65479T=DSM 105299T) can be classified as the type strain of a novel species of the genus Streptomyces, for which the name Streptomyces rhizosphaericola sp. nov. is proposed.


Asunto(s)
Filogenia , Rizosfera , Microbiología del Suelo , Streptomyces/clasificación , Triticum/microbiología , Técnicas de Tipificación Bacteriana , Composición de Base , Brasil , ADN Bacteriano/genética , Ácidos Grasos/química , Genes Bacterianos , Tipificación de Secuencias Multilocus , Fosfolípidos/química , ARN Ribosómico 16S/genética , Análisis de Secuencia de ADN , Streptomyces/aislamiento & purificación
2.
Antonie Van Leeuwenhoek ; 111(4): 629-636, 2018 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-29143212

RESUMEN

A novel actinobacterium, designated strain CMAA 1533T, was isolated from the rhizosphere of Deschampsia antarctica collected at King George Island, Antarctic Peninsula. Strain CMAA 1533T was found to grow over a wide range of temperatures (4-28 °C) and pH (4-10). Macroscopically, the colonies were observed to be circular shaped, smooth, brittle and opaque-cream on most of the culture media tested. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain CMAA 1533T belongs to the family Nocardiaceae and forms a distinct phyletic line within the genus Rhodococcus. Sequence similarity calculations indicated that the novel strain is closely related to Rhodococcus degradans CCM 4446T, Rhodococcus erythropolis NBRC 15567T and Rhodococcus triatomae DSM 44892T (≤ 96.9%). The organism was found to contain meso-diaminopimelic acid, galactose and arabinose in whole cell hydrolysates. Its predominant isoprenologue was identified as MK-8(H2) and the polar lipids as diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and phosphatidylinositol mannosides. The major fatty acids were identified as Summed feature (C16:1 ω6c and/or C16:1 ω7c), C16:0, C18:1 ω9c and 10-methyl C18:0. The G+C content of genomic DNA was determined to be 65.5 mol%. Unlike the closely related type strains, CMAA 1533T can grow at 4 °C but not at 37 °C and was able to utilise adonitol and galactose as sole carbon sources. Based on phylogenetic, chemotaxonomic and physiological data, it is concluded that strain CMAA 1533T (= NRRL B-65465T = DSM 104532T) represents a new species of the genus Rhodococcus, for which the name Rhodococcus psychrotolerans sp. nov. is proposed.


Asunto(s)
Filogenia , Poaceae/microbiología , Rizosfera , Rhodococcus/clasificación , Microbiología del Suelo , Regiones Antárticas , Composición de Base , Metabolismo de los Hidratos de Carbono , Ácido Diaminopimélico/análisis , Ácidos Grasos/análisis , Genoma Bacteriano/genética , Peptidoglicano/química , Fosfolípidos/análisis , ARN Ribosómico 16S/genética , Rhodococcus/química , Rhodococcus/genética , Rhodococcus/metabolismo , Especificidad de la Especie , Temperatura , Vitamina K 2/análogos & derivados , Vitamina K 2/análisis
3.
Int J Syst Evol Microbiol ; 67(6): 2019-2025, 2017 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-28632117

RESUMEN

A novel marine actinomycete, designated strain CMAA 1452T, was isolated from the sponge Scopalina ruetzleri collected from Saint Peter and Saint Paul Archipelago, in Brazil, and subjected to a polyphasic taxonomic investigation. The organism formed a distinct phyletic line in the Saccharopolyspora 16S rRNA gene tree and had chemotaxonomic and morphological properties consistent with its classification in this genus. It was found to be closely related to Saccharopolyspora dendranthemae KLBMP 1305T (99.5% 16S rRNA gene sequence similarity) and shared similarities of 99.3, 99.2 and 99.0 % with 'Saccharopolyspora endophytica' YIM 61095, Saccharopolyspora tripterygii YIM 65359T and 'Saccharopolyspora pathumthaniensis' S582, respectively. DNA-DNA relatedness values between the isolate and its closest phylogenetic neighbours, namely S. dendranthemae KLBMP 1305T, 'S. endophytica' YIM 61095 and S. tripterygii YIM 65359T, were 53.5, 25.8 and 53.2 %, respectively. Strain CMAA 1452T was also distinguished from the type strains of these species using a range of phenotypic features. On the basis of these results, it is proposed that strain CMAA 1452T (=DSM 103218T=NRRL B-65384T) merits recognition as the type strain of a novel Saccharopolyspora species, Saccharopolyspora spongiae sp. nov.


Asunto(s)
Filogenia , Poríferos/microbiología , Saccharopolyspora/clasificación , Animales , Técnicas de Tipificación Bacteriana , Composición de Base , Brasil , ADN Bacteriano/genética , Hibridación de Ácido Nucleico , ARN Ribosómico 16S/genética , Saccharopolyspora/genética , Saccharopolyspora/aislamiento & purificación , Análisis de Secuencia de ADN
4.
Antonie Van Leeuwenhoek ; 109(2): 319-34, 2016 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-26809280

RESUMEN

The taxonomic position of 26 filamentous actinobacteria isolated from a hyper-arid Atacama Desert soil and 2 from an arid Australian composite soil was established using a polyphasic approach. All of the isolates gave the diagnostic amplification product using 16S rRNA oligonucleotide primers specific for the genus Amycolatopsis. Representative isolates had chemotaxonomic and morphological properties typical of members of the genus Amycolatopsis. 16S rRNA gene analyses showed that all of the isolates belong to the Amycolatopsis methanolica 16S rRNA gene clade. The Atacama Desert isolates were assigned to one or other of two recognised species, namely Amycolatopsis ruanii and Amycolatopsis thermalba, based on 16S rRNA gene sequence, DNA:DNA relatedness and phenotypic data; emended descriptions are given for these species. In contrast, the two strains from the arid Australian composite soil, isolates GY024(T) and GY142, formed a distinct branch at the periphery of the A. methanolica 16S rRNA phyletic line, a taxon that was supported by all of the tree-making algorithms and by a 100 % bootstrap value. These strains shared a high degree of DNA:DNA relatedness and have many phenotypic properties in common, some of which distinguished them from all of the constituent species classified in the A. methanolica 16S rRNA clade. Isolates GY024(T) and GY142 merit recognition as a new species within the A. methanolica group of thermophilic strains. The name proposed for the new species is Amycolatopsis deserti sp. nov.; the type strain is GY024(T) (=NCIMB 14972(T) = NRRL B-65266(T)).


Asunto(s)
Actinomycetales/aislamiento & purificación , Microbiología del Suelo , Actinomycetales/clasificación , Actinomycetales/genética , Actinomycetales/metabolismo , Australia , ADN Bacteriano/genética , ADN Ribosómico/genética , Clima Desértico , Calor , Datos de Secuencia Molecular , Filogenia , ARN Ribosómico 16S/genética , Suelo/química
5.
Int J Syst Evol Microbiol ; 65(7): 2286-2291, 2015 Jul.
Artículo en Inglés | MEDLINE | ID: mdl-26231541

RESUMEN

A novel marine actinomycete, designated B374(T), was isolated from a marine sponge, Glodia corticostylifera, which was collected from São Paulo, Brasil. The taxonomic position of B374(T) was established by using data derived from a polyphasic approach. The organism showed a combination of chemotaxonomic and morphological characteristics consistent with its classification in the genus Marmoricola and it formed a distinct phyletic line in the clade of the genus Marmoricola, based on 16S rRNA gene sequences. Strain B374(T) was most closely related to Marmoricola aequoreus SST-45(T) (98.5% 16S rRNA gene sequence similarity), but was distinguished from this strain and from the other type strains of species of the genus Marmoricola on the basis of a combination of phenotypic properties. The data obtained, therefore, indicates that isolate B374(T) ( = CBMAI 1089(T) = DSM 28169(T)) should be classified as a novel species of the genus Marmoricola, for which the name Marmoricola aquaticus sp. nov. is proposed.


Asunto(s)
Actinomycetales/clasificación , Filogenia , Poríferos/microbiología , Actinomycetales/genética , Actinomycetales/aislamiento & purificación , Animales , Composición de Base , Brasil , ADN Bacteriano/genética , Ácidos Grasos/química , Datos de Secuencia Molecular , Hibridación de Ácido Nucleico , ARN Ribosómico 16S/genética , Análisis de Secuencia de ADN
6.
Phytopathology ; 104(12): 1298-305, 2014 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-24983843

RESUMEN

A recently described actinomycete species (Streptomyces araujoniae ASBV-1(T)) is effective against many phytopathogenic fungi. In this study, we evaluated the capacity of this species to inhibit Botrytis cinerea development in strawberry pseudofruit, and we identified the chemical structures of its bioactive compounds. An ethyl acetate crude extract (0.1 mg ml(-1)) of ASBV-1(T) fermentation broth completely inhibited fungus growth in strawberry pseudofruit under storage conditions. The crude extract was fractionated by preparative high-performance liquid chromatography; the active fraction was further evaluated by tandem mass spectrometry. ASBV-1(T) produced a multiantibiotic complex with ionophoric properties. This complex contained members of the macrotetralides class (including monactin, dinactin, trinactin, and tetranactin) and the cyclodepsipeptide valinomycin, all of which were active against B. cinerea. Furthermore, the addition of 2 mM MgSO4 and 1 mM ZnSO4 enhanced macrotetralide and valinomycin production, respectively, in the culture broth. These compounds are considered to be the main active molecules that S. araujoniae produces to control B. cinerea. Their low to moderate toxicity to humans and the environment justifies the application of ASBV-1(T) in biological control programs that aim to mitigate the damage caused by this phytopathogen.


Asunto(s)
Antiinfecciosos/farmacología , Botrytis/efectos de los fármacos , Fragaria/microbiología , Control Biológico de Vectores , Enfermedades de las Plantas/prevención & control , Streptomyces/química , Acetatos , Antiinfecciosos/química , Antiinfecciosos/aislamiento & purificación , Frutas/microbiología , Enfermedades de las Plantas/microbiología , Streptomyces/aislamiento & purificación , Streptomyces/fisiología
7.
Antonie Van Leeuwenhoek ; 103(6): 1235-44, 2013 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-23504246

RESUMEN

The taxonomic position of a streptomycete isolated from a potato tubercle was determined by using a polyphasic approach. The organism had chemotaxonomic and morphological properties consistent with its classification in the genus Streptomyces and formed a distinct phyletic line in the Streptomyces 16S rRNA gene tree. It was found to be closely related to Streptomyces celluloflavus NRRL B-2493(T) (99.4 % 16S rRNA gene similarity) and shared a 99.0 % 16S rRNA gene similarity value with Streptomyces albolongus NRRL B-3604(T) and Streptomyces cavourensis subsp. cavourensis NBRC 13026(T); low levels of DNA-DNA relatedness with these organisms showed that the isolate belonged to a distinct genomic species. The isolate was distinguished readily from the type strains of these species using a combination of morphological and other phenotypic properties. On the basis of these results, it is proposed that isolate ASBV-1(T) (= CBMAI 1465(T) = CCMA 894(T) = NRRL B-24922(T)) be classified as the type strain of Streptomyces araujoniae sp. nov.


Asunto(s)
Solanum tuberosum/microbiología , Streptomyces/clasificación , Streptomyces/aislamiento & purificación , Antibiosis , Aspergillus/crecimiento & desarrollo , Técnicas de Tipificación Bacteriana , Secuencia de Bases , ADN Bacteriano/genética , Genes de ARNr , Datos de Secuencia Molecular , Filogenia , ARN Ribosómico 16S/genética , Análisis de Secuencia de ADN , Microbiología del Suelo , Streptomyces/genética
8.
Sci Rep ; 10(1): 13870, 2020 08 17.
Artículo en Inglés | MEDLINE | ID: mdl-32807803

RESUMEN

Although many advances have been achieved to treat aggressive tumours, cancer remains a leading cause of death and a public health problem worldwide. Among the main approaches for the discovery of new bioactive agents, the prospect of microbial secondary metabolites represents an effective source for the development of drug leads. In this study, we investigated the actinobacterial diversity associated with an endemic Antarctic species, Deschampsia antarctica, by integrated culture-dependent and culture-independent methods and acknowledged this niche as a reservoir of bioactive strains for the production of antitumour compounds. The 16S rRNA-based analysis showed the predominance of the Actinomycetales order, a well-known group of bioactive metabolite producers belonging to the Actinobacteria phylum. Cultivation techniques were applied, and 72 psychrotolerant Actinobacteria strains belonging to the genera Actinoplanes, Arthrobacter, Kribbella, Mycobacterium, Nocardia, Pilimelia, Pseudarthrobacter, Rhodococcus, Streptacidiphilus, Streptomyces and Tsukamurella were identified. The secondary metabolites were screened, and 17 isolates were identified as promising antitumour compound producers. However, the bio-guided assay showed a pronounced antiproliferative activity for the crude extracts of Streptomyces sp. CMAA 1527 and Streptomyces sp. CMAA 1653. The TGI and LC50 values revealed the potential of these natural products to control the proliferation of breast (MCF-7), glioblastoma (U251), lung/non-small (NCI-H460) and kidney (786-0) human cancer cell lines. Cinerubin B and actinomycin V were the predominant compounds identified in Streptomyces sp. CMAA 1527 and Streptomyces sp. CMAA 1653, respectively. Our results suggest that the rhizosphere of D. antarctica represents a prominent reservoir of bioactive actinobacteria strains and reveals it as an important environment for potential antitumour agents.


Asunto(s)
Actinobacteria , Técnicas de Cultivo/métodos , Descubrimiento de Drogas , Neoplasias/patología , Actinobacteria/metabolismo , Actinomycetales/metabolismo , Regiones Antárticas , Antraciclinas/aislamiento & purificación , Antraciclinas/metabolismo , Antraciclinas/farmacología , Antineoplásicos/aislamiento & purificación , Antineoplásicos/metabolismo , Antineoplásicos/farmacología , Factores Biológicos/biosíntesis , Factores Biológicos/aislamiento & purificación , Factores Biológicos/farmacología , Línea Celular Tumoral , Proliferación Celular/efectos de los fármacos , Dactinomicina/biosíntesis , Dactinomicina/aislamiento & purificación , Dactinomicina/farmacología , Humanos , Streptomyces/metabolismo
9.
Rev. epidemiol. controle infecç ; 12(4): 180-183, out.-dez. 2022. ilus
Artículo en Inglés, Portugués | LILACS | ID: biblio-1425989

RESUMEN

Background and objectives: In 2015, Microsoft launched a Business Intelligence service, called Power BI, which can be used in several areas of knowledge if operated by a qualified professional. Power BI has several utilities, including the integration of data from various sources and formats, case mapping, real-time data visualizations, and remote work. This study aimed to report the use experience of Power BI by utilizing data from a State Department of Health Western Amazon, Brazil. Methods: As an example, cases of tuberculosis in the state of Acre, from 2010 to 2020, were used. These data wee extracted from the Notifiable Diseases Information System, provided by the State Health Department of Acre ­ SESACRE. Results: Power BI offers a simple and intuitive interface. To share the experience, we provided a link (http://tiny.cc/tbacre) to explore the tool and understand the speed and practicality in data visualization. Conclusion: We recommend the use of Power BI mainly in agencies that need agile decision-making based on evidence.(AU)


Justificativa e objetivos: Em 2015, a Microsoft lançou um serviço de Business Intelligence (Inteligência de Negócios), chamado Power BI, que pode ser empregado em diversas áreas do conhecimento, desde que operado por um profissional capacitado. Possui diversas utilidades, entre elas: a integração de dados provenientes de diversas fontes e formatos, mapeamento de casos, visualizações de dados em tempo real e trabalho remoto. Este trabalho propôs relatar a experiência de uso do Microsoft Power BI utilizando dados de uma Secretaria Estadual de Saúde da Amazônia Ocidental, Brasil. Métodos: Como exemplo, utilizam-se casos de tuberculose no estado do Acre, de 2010 a 2020, extraídos do Sistema de Informação de Agravos de Notificação (Sinan), fornecidos pela Secretaria de Estado da Saúde do Acre (Sesacre). Resultados: O Power BI oferece uma interface simples e intuitiva. Para compartilhar a experiência, foi fornecido um link (http://tiny.cc/tbacre) por meio do qual foi possível explorar a ferramenta e perceber a rapidez e praticidade na visualização de dados. Conclusão: Recomenda-se sua utilização sobretudo em órgãos que necessitam de tomadas ágeis de decisões baseadas em evidências.(AU)


Justificación y objetivos: En 2015, Microsoft lanzó un servicio de Business Intelligence, denominado Power BI, que puede ser utilizado en diversas áreas del conocimiento siempre que sea realizado por un profesional capacitado. Tiene varias utilidades, incluyendo la integración de datos de diferentes fuentes y formatos, mapeo de casos, visualización de datos en tiempo real y trabajo remoto. Este trabajo propuso relatar la experiencia de uso de Microsoft Power BI utilizando datos de un departamento de salud estatal en la Amazonía Occidental, Brasil. Methods: Como ejemplo, se utilizaron casos de tuberculosis en el estado de Acre, de 2010 a 2020, extraídos del Sistema de Información de Enfermedades de Declaración Obligatoria ­ SINAN, proporcionado por la Secretaría de Salud del Estado de Acre ­ SESACRE. Results: Power BI ofrece una interfaz sencilla e intuitiva. Para compartir la experiencia, se facilitó el enlace (http://tiny.cc/tbacre). A través de esto, fue posible explorar la herramienta y darse cuenta de la rapidez y practicidad en la visualización de datos. Conclusión: Recomendamos su uso, sobre todo, en órganos que necesiten una toma de decisiones ágil y basada en evidencias.(AU)


Asunto(s)
Control de Enfermedades Transmisibles , Gestión en Salud , Tecnología de la Información , Monitoreo Epidemiológico , Gestión de la Información en Salud
10.
Cien Saude Colet ; 21(4): 1095-103, 2016 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-27076008

RESUMEN

The current study evaluated physical activity (PA) level and its associated effects among students in the public network of São Paulo, Brazil. A cross-sectional study was taken using a representative sample of students in São Paulo public school system. International Physical Activity Questionnaire determined PA level. Students who reported at least 300 minutes/week of PA were considered active. The independente variables were: gender, age, body mass index, education, region, recognition of the Agita São Paulo program. The prevalence of regular PA was 71.7%. Males (PR = 1.09, 95% CIs = 1.04 -1.15) at least 17 years old (PR = 1.16, 95% CIs 1.09-1.24) in their 3rd year of high school (PR = 1.20, 95% CIs = 1.12-1.29) who resided in the Midwest region (PR = 1.27, 95% CIs 1.16-1.38) were most likely to be active. Males at least 17 years old in their 3rd year of high school who lived in the Midwest and recognized the Agita São Paulo/Agita Galera program had higher levels of PA. São Paulo students presented a high level of PA. Moreover, males older than 17 years, attending their 3rd year of high school, who lived in the Midwest region, and recognized the Agita São Paulo/Agita Galera program were the most likely to be more active.


Asunto(s)
Ejercicio Físico , Estudiantes , Adolescente , Brasil/epidemiología , Niño , Estudios Transversales , Femenino , Humanos , Masculino , Prevalencia
11.
Rev Bras Ortop ; 48(1): 46-51, 2013.
Artículo en Inglés | MEDLINE | ID: mdl-31304110

RESUMEN

OBJECTIVE: To assess the association between malalignment of the knees (genu valgum) and variables of physical fitness among schoolchildren. METHODS: We analyzed data collected between the years 2000 to 2009. The sample comprised 1,141 schoolchildren of both sexes aged 06 to 18 years. To participate in the research, the students must meet the following criteria: age between 6 and 18 years and a full assessment of physical fitness, including measurement of genu valgus in at least one of the semester assessments. Postural evaluation (valgus) was determined by the intermalleolar distance, in centimeter. Body Mass Index (BMI) determined through the growth curves of the World Health Organization. Physical fitness variables (strength of upper and lower limbs, agility, speed and flexibility), were taken according to CELAFISCS standardization. RESULTS: Among male students it was found a prevalence of 23.2% obese, 44.4% overweight and 32.4% eutrophic. Among females, the values were: 30.9% obese, overweight 39.5% and 20.6% eutrophic. When analyzing the prevalence of valgus according to the BMI classifications it was, found a significant positive association in both sexes. Association was found between genu valgum and upper limbs strength only in crude analysis. The other variables, agility, speed and flexibility were not associated even when the analysis was adjusted. CONCLUSION: there was a positive association between malalignment of the knees, body mass index and physical fitness among schoolchildren.

12.
Cad Saude Publica ; 28(3): 463-71, 2012 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-22415178

RESUMEN

The aim of this study was to determine the association between levels of physical activity and usage of medication in older women. The level of physical activity was assessed using a pedometer. Use of medication was assessed through medical records supplied in reports kept by the Family Health Program, City Health Department, São Caetano do Sul, São Paulo State, Brazil. Regular use of pharmaceuticals, regardless of type of illness or treatment, was listed. Data analysis was performed using Poisson regression to estimate the prevalence ratio. The results of the study indicated that, amongst the 271 eligible women, 84.9% had been classified as active. Only 23.2% did not use any type of medication while 29.8% used three or more medications. The level of physical activity was inversely associated with the number of medications used, under both crude analysis and after adjustment. The study concluded that higher volumes of physical activity were significantly associated with lower usage of pharmaceuticals in women who are involved in a physical activity program.


Asunto(s)
Quimioterapia/estadística & datos numéricos , Ejercicio Físico/fisiología , Factores de Edad , Anciano , Anciano de 80 o más Años , Brasil , Femenino , Humanos , Persona de Mediana Edad , Distribución de Poisson , Factores Socioeconómicos , Factores de Tiempo
13.
J Pediatr (Rio J) ; 88(3): 239-45, 2012 May.
Artículo en Inglés | MEDLINE | ID: mdl-22622801

RESUMEN

OBJECTIVE: To analyze adiposity changes in school-age children over a 20 year-period, according to nutritional status. METHODS: The study is part of the Ilhabela Longitudinal Mixed Project on Growth, Development and Physical Fitness. A sample of 1,095 school students of both sexes, from 7 to 10 years, met the following inclusion criteria: (a) at least one complete assessment in one of the analyzed periods; (b) to be in prepubertal stage of sexual maturation; (c) to be apparently healthy. The periods analyzed were 1990/1991 (initial), 2000/2001 (10 years) and 2010/2011 (20 years). The variables analyzed were: body weight, height and adiposity through individual analysis of each skinfold. Children were classified as eutrophic, overweight and obese, according to the curves of body mass index for age and sex proposed by the World Health Organization. The statistical analysis used was one-way ANOVA, followed by Scheffé's post-hoc test, with p < 0.01. RESULTS: In boys, the largest increase occurred in the overweight group, followed by the obese and eutrophic groups. In girls, the largest increases occurred in the groups with overweight and eutrophic children, followed by the obese group. CONCLUSION: During the 20-year period analyzed, there were changes in adiposity, even when the nutritional status was controlled, showing that individuals who have similar body mass indexes may vary in proportion and distribution of subcutaneous adipose tissue. In both sexes, the increase was higher in the overweight group, and mainly in central skinfolds.


Asunto(s)
Adiposidad/fisiología , Estado Nutricional/fisiología , Obesidad Abdominal/epidemiología , Grosor de los Pliegues Cutáneos , Adolescente , Análisis de Varianza , Índice de Masa Corporal , Brasil/epidemiología , Niño , Femenino , Humanos , Estudios Longitudinales , Masculino , Instituciones Académicas , Factores Sexuales , Factores de Tiempo
14.
Ciênc. Saúde Colet. (Impr.) ; 21(4): 1095-1103, Abr. 2016. tab, graf
Artículo en Inglés | LILACS | ID: lil-778588

RESUMEN

Abstract The current study evaluated physical activity (PA) level and its associated effects among students in the public network of São Paulo, Brazil. A cross-sectional study was taken using a representative sample of students in São Paulo public school system. International Physical Activity Questionnaire determined PA level. Students who reported at least 300 minutes/week of PA were considered active. The independente variables were: gender, age, body mass index, education, region, recognition of the Agita São Paulo program. The prevalence of regular PA was 71.7%. Males (PR = 1.09, 95% CIs = 1.04 -1.15) at least 17 years old (PR = 1.16, 95% CIs 1.09-1.24) in their 3rd year of high school (PR = 1.20, 95% CIs = 1.12-1.29) who resided in the Midwest region (PR = 1.27, 95% CIs 1.16-1.38) were most likely to be active. Males at least 17 years old in their 3rd year of high school who lived in the Midwest and recognized the Agita São Paulo/Agita Galera program had higher levels of PA. São Paulo students presented a high level of PA. Moreover, males older than 17 years, attending their 3rd year of high school, who lived in the Midwest region, and recognized the Agita São Paulo/Agita Galera program were the most likely to be more active.


Resumo O presente estudo avaliou nível de atividade física (AF) e seus efeitos associados entre estudantes da rede pública de São Paulo. Um estudo transversal foi feito usando uma amostra representativa de estudantes da rede pública de ensino de São Paulo. Foi utilizado o International Physical Activity Questionnaire para determinar o nível AF. Estudantes que relataram pelo menos 300 minutos/semana de AF, foram considerados ativos. As variáveis independentes foram: sexo, idade, índice de massa corporal, educação, região e o conhecimento do programa Agita São Paulo. A prevalência de AF regular foi de 71,7%. Os meninos (RP = 1,09, IC95% = 1,04 -1,15), 17 anos de idade (RP = 1,16, 95% IC 1,09-1,24), terceiro ano do ensino médio (RP = 1,20, IC95% = 1,12-1,29 ) e aqueles que residiam na região do Centro-Oeste (RP = 1,27, IC95% 1,16-1,38) tinham maior probabilidade de ser ativos. Meninos de 17 anos de idade, no terceiro ano do ensino médio, que residiam no Centro-Oeste e reconhecia o programa Agita São Paulo/Agita Galera tinham níveis mais elevados de AF. Alunos de São Paulo apresentaram um elevado nível de AF. Meninos com idade superior a 17 anos, do 3º ano do ensino médio, que residiam na região Centro-Oeste, e reconhecia o programa Agita São Paulo/Agita Galera foram mais propensos a ser mais ativos.


Asunto(s)
Humanos , Masculino , Femenino , Niño , Adolescente , Estudiantes , Ejercicio Físico , Brasil/epidemiología , Prevalencia , Estudios Transversales
15.
Rev. bras. ortop ; 48(1): 46-51, Jan-Feb/2013. tab, graf
Artículo en Inglés | LILACS | ID: lil-674581

RESUMEN

OBJECTIVE: To assess the association between malalignment of the knees (genu valgum) and variables of physical fitness among schoolchildren. METHODS: We analyzed data collected between the years 2000 to 2009. The sample comprised 1,141 schoolchildren of both sexes aged 06 to 18 years. To participate in the research, the students must meet the following criteria: age between 6 and 18 years and a full assessment of physical fitness, including measurement of genu valgus in at least one of the semester assessments. Postural evaluation (valgus) was determined by the intermalleolar distance, in centimeter. Body Mass Index (BMI) determined through the growth curves of the World Health Organization. Physical fitness variables (strength of upper and lower limbs, agility, speed and flexibility), were taken according to CELAFISCS standardization. RESULTS: Among male students it was found a prevalence of 23.2% obese, 44.4% overweight and 32.4% eutrophic. Among females, the values were: 30.9% obese, overweight 39.5% and 20.6% eutrophic. When analyzing the prevalence of valgus according to the BMI classifications it was, found a significant positive association in both sexes. Association was found between genu valgum and upper limbs strength only in crude analysis. The other variables, agility, speed and flexibility were not associated even when the analysis was adjusted. CONCLUSION: there was a positive association between malalignment of the knees, body mass index and physical fitness among schoolchildren.


OBJETIVO: Verificar associação entre o mau alinhamento de joelhos (geno valgo) e variáveis de aptidão física em estudantes. MÉTODOS: Foram analisados dados coletados entre 2000 a 2009. A amostra foi composta por 1.141 estudantes de ambos os sexos, de 6 a 18 anos. Para participar da pesquisa os estudantes deveriam atender os seguintes critérios: ter entre 6 e 18 anos e uma avaliação completa da aptidão física, incluindo a medida de geno valgo. O valgismo foi determinado pela distância intermaleolar, com régua em centímetros. Já para a classificação do Índice de Massa Corporal (IMC) usaram-se as curvas de crescimento da Organização Mundial da Saúde e as variáveis de aptidão física analisadas (força de membros inferiores e superiores, agilidade, velocidade e flexibilidade) seguiram a padronização CELAFISCS. RESULTADOS: No sexo masculino foi encontra uma prevalência de 23,2% de obesos, 44,4% com excesso de peso e 32,4% eutróficos. Entre o sexo feminino, 30,9% eram obesas, 39,5% com excesso de peso 39,5% e 20,6% eutróficas. Quando analisada a prevalência de valgismo de acordo com as classificações do IMC, foi encontrada associação positiva e significativa em ambos os sexos. Foi encontrada associação entre geno valgo à força de membros superiores apenas na análise bruta. As demais variáveis não apresentaram associação. CONCLUSÃO: Houve associação positiva entre mau alinhamento dos joelhos, índice de massa corporal e aptidão física em estudantes.


Asunto(s)
Humanos , Masculino , Femenino , Niño , Adolescente , Adulto Joven , Índice de Masa Corporal , Genu Valgum , Aptitud Física
16.
Cad. saúde pública ; 28(3): 463-471, mar. 2012. graf, tab
Artículo en Inglés | LILACS | ID: lil-616959

RESUMEN

The aim of this study was to determine the association between levels of physical activity and usage of medication in older women. The level of physical activity was assessed using a pedometer. Use of medication was assessed through medical records supplied in reports kept by the Family Health Program, City Health Department, São Caetano do Sul, São Paulo State, Brazil. Regular use of pharmaceuticals, regardless of type of illness or treatment, was listed. Data analysis was performed using Poisson regression to estimate the prevalence ratio. The results of the study indicated that, amongst the 271 eligible women, 84.9 percent had been classified as active. Only 23.2 percent did not use any type of medication while 29.8 percent used three or more medications. The level of physical activity was inversely associated with the number of medications used, under both crude analysis and after adjustment. The study concluded that higher volumes of physical activity were significantly associated with lower usage of pharmaceuticals in women who are involved in a physical activity program.


O objetivo deste estudo foi avaliar a associação entre o nível de atividade física e uso de medicamentos em mulheres com 60 anos de idade ou mais. O nível de atividade física foi avaliado utilizando pedômetro. O consumo de medicamento foi avaliado mediante o prontuário de cadastro da Estratégia Saúde da Família em São Caetano do Sul, São Paulo, Brasil. Foram registrados os medicamentos de uso regular independente do tipo da doença ou do tratamento. Foi realizada análise de regressão de Poisson para estimar a razão de prevalências. Das 271 mulheres elegíveis, 84,9 por cento foram classificadas como ativas. Apenas 23,2 por cento não utilizam nenhum tipo de medicamento, enquanto 29,8 por cento utilizaram três ou mais medicamentos. O nível de atividade física foi inversamente associado com o número de medicamentos utilizados tanto na análise bruta como na ajustada. Maiores volumes de atividade física associaram-se significativamente com menor consumo de medicamentos em mulheres envolvidas em um programa de atividade física.


Asunto(s)
Anciano , Anciano de 80 o más Años , Femenino , Humanos , Persona de Mediana Edad , Quimioterapia , Ejercicio Físico/fisiología , Factores de Edad , Brasil , Distribución de Poisson , Factores Socioeconómicos , Factores de Tiempo
17.
J. pediatr. (Rio J.) ; 88(3): 239-245, maio-jun. 2012. tab
Artículo en Portugués | LILACS | ID: lil-640779

RESUMEN

OBJETIVO: Analisar as modificações da adiposidade de escolares durante 20 anos, de acordo com o estado nutricional. MÉTODOS: O estudo faz parte do Projeto Misto Longitudinal de Crescimento, Desenvolvimento e Aptidão Física de Ilhabela. Uma amostra de 1.095 escolares de ambos os sexos, de 7 a 10 anos, atendiam aos seguintes critérios de inclusão: (a) pelo menos uma avaliação completa em um dos períodos analisados; (b) estar no estágio pré-púbere de maturação sexual; (c) ser aparentemente saudável. Os períodos analisados foram 1990/1991 (inicial), 2000/2001 (10 anos) e 2010/2011 (20 anos). As variáveis analisadas foram: massa corporal, estatura e adiposidade, pela análise individual de cada dobra cutânea. Os indivíduos foram classificados em eutróficos, com excesso de peso e obesos mediante as curvas propostas pela Organização Mundial da Saúde de índice de massa corporal para idade e sexo. Foi utilizada a Anova one-way, seguida do post-hoc de Scheffé, com p < 0,01. RESULTADOS: Nos meninos, o maior aumento ocorreu no grupo com excesso de peso, seguido pelos grupos obesos e eutróficos. Já nas meninas, os maiores aumentos ocorreram nos grupos com excesso de peso e eutróficas, seguidos pelo grupo obesas. CONCLUSÃO: Houve modificações da adiposidade, mesmo com o controle do estado nutricional, durante o período de 20 anos analisado, mostrando que os indivíduos que têm semelhantes índices de massa corporal podem variar em proporção e distribuição de tecido adiposo subcutâneo. Para ambos os sexos, o aumento foi maior nos grupos classificados como excesso de peso e, principalmente, nas dobras cutâneas centrais.


OBJECTIVE: To analyze adiposity changes in school-age children over a 20 year-period, according to nutritional status. METHODS: The study is part of the Ilhabela Longitudinal Mixed Project on Growth, Development and Physical Fitness. A sample of 1,095 school students of both sexes, from 7 to 10 years, met the following inclusion criteria: (a) at least one complete assessment in one of the analyzed periods; (b) to be in prepubertal stage of sexual maturation; (c) to be apparently healthy. The periods analyzed were 1990/1991 (initial), 2000/2001 (10 years) and 2010/2011 (20 years). The variables analyzed were: body weight, height and adiposity through individual analysis of each skinfold. Children were classified as eutrophic, overweight and obese, according to the curves of body mass index for age and sex proposed by the World Health Organization. The statistical analysis used was one-way ANOVA, followed by Scheffé's post-hoc test, with p < 0.01. RESULTS: In boys, the largest increase occurred in the overweight group, followed by the obese and eutrophic groups. In girls, the largest increases occurred in the groups with overweight and eutrophic children, followed by the obese group. CONCLUSION: in adiposity, even when the nutritional status was controlled, showing that individuals who have similar body mass indexes may vary in proportion and distribution of subcutaneous adipose tissue. In both sexes, the increase was higher in the overweight group, and mainly in central skinfolds.


Asunto(s)
Adolescente , Niño , Femenino , Humanos , Masculino , Adiposidad/fisiología , Estado Nutricional/fisiología , Obesidad Abdominal/epidemiología , Grosor de los Pliegues Cutáneos , Análisis de Varianza , Índice de Masa Corporal , Brasil/epidemiología , Estudios Longitudinales , Instituciones Académicas , Factores Sexuales , Factores de Tiempo
18.
Artículo en Portugués | LILACS-Express | LILACS | ID: lil-574548

RESUMEN

O objetivo da pesquisa foi verificar o efeito de um programa de atividade física e estabilidade das variáveis de aptidão física e capacidade funcional de mulheres adultas fisicamente ativas. A amostra foi composta por 34 mulheres entre 50-89 anos, participantes de um grupo de atividade física da Universidade Camilo Castelo Branco. Critérios de inclusão: adesão mínima de 75% de frequência às aulas (3x/semana) e ter realizado, nomínimo, uma avaliação por ano entre 2005-2007. Todas as avaliações foram realizadas no mês de junho. As medidas antropométricas foram: massa corporal, estatura, circunferência da cintura e quadril; neuromotoras: levantar da cadeira em segundos, levantar da cadeira em30 segundos, flexão de cotovelo, shuttle-run, equilíbrio estático e dinâmico; e metabólica: marcha estacionária de 2 minutos, foi seguida a padronização CELAFISCS. A análise dos dados foi feita pela ANOVA - One Way seguida do ?post hoc de Scheffé?, delta percentual e correlação de Spearman Rho. O nível de significância adotado foi o p<0,05. Nas trêsavaliações realizadas, a massa corporal, índice de massa corporal e relação cintura quadril indicaram valores de excesso de peso. A capacidade funcional demonstrou incremento na força de membros superiores (42%) e inferiores (5,2%) e para o equilíbrio (14,1%), essas mudanças foram significativas, o mesmo não aconteceu para agilidade (2,1%), isso comparando 2005-2007. O índice de massa corporal, relação cintura quadril, força e equilíbrio estático a estabilidade variou de 0,26 a 0,91 (p<0,05). O programa de atividade física contribuiu para a manutenção da aptidão física e capacidade funcional de mulheres adultas.


The objective of this study was to evaluate the effect of a physical activity program and [on ?] the stability of physical fitness and functional capacity in physically active adult women. Thirty-four adult women aged 50-89 years, who participated in a physical activity group at Camilo Castelo Branco University, were studied. Criteria for inclusion were attendanceof at least 75% of the classes (3x/week) and undergoing at least one assessment per year between 2005 and 2007. All evaluations were conducted in June. Anthropometric (body weight, height, waist-hip ratio), neuromotor (rising from a chair in seconds, rising from a chair in 30 seconds, elbow flexion, shuttle-run, static and dynamic balance), and metabolic parameters (2-min step in place test according to the CELAFISCS standard) were evaluated. One-way ANOVA followed by the post hoc Scheffé test, delta percentage and Spearman?s rho correlation were used for data analysis, with the level of significance set at p<0.05. In the three evaluations, body weight, body mass index and waist-hip ratio indicated overweight. Analysis of functional capacity showed a significant increase in upper (42%) and lower (5.2%) limb strength and in balance (14.1%) when comparing the results from 2005 to 2007. The samewas not observed for agility (2.1%). For body mass index, waist-hip ratio, strength and static balance, stability ranged from 0.26 to 0.91 (p<0.05). The physical activity program contributed to the maintenance of physical fitness and functional capacity in adult women.

19.
Rev. bras. ciênc. mov ; 16(4): 1-18, jan.-mar. 2008. tab, graf
Artículo en Portugués | LILACS | ID: lil-727507

RESUMEN

O objetivo deste estudo foi analisar o impacto de um programa supervisionado de caminhada sobre o nível de atividade física e mudança dos estágios de comportamento na comunidade. A amostra foi composta por 114 indivíduos, de ambos os gêneros, com média de idade de 38,14±11,41 anos. O presente estudo foi dividido em dois momentos, totalizando 33 semanas, sendo: 16 semanas com orientação de um profissional e 17 semanas sem supervisão do grupo de caminhada por um profissional. Foi utilizada análise descritiva, análise de variância não paramétrica, delta percentual, Qui-Quadrado e análise de regressão univariada, sendo o nível de significância adotado de p<0,05. Foram classificados como insuficientemente ativos 68% dos indivíduos e apenas 32% como suficientemente ativos. Após o período de orientação foi observado 43% de indivíduos insuficientemente e 57% indivíduos suficientemente ativos. Após 17 semanas sem supervisão as proporções foram parecidas com o momento de orientação, sendo estas diferentes do “baseline” (p<0,05). Foi observada uma diminuição de 97,14% entre os indivíduos classificados como pré-contemplativos entre o momento inicial e o momento de orientação e aumento de 96,3% nos indivíduos classificados no estágio de manutenção no mesmo período.. Programas não supervisionados de caminhada aumentam o nível de atividade física. O conhecimento adquirido durante o processo de orientação e o desenvolvimento da habilidade para a caminhada possivelmente influenciaram na manutenção do nível de atividade física a curto e médio prazo após a suspensão da orientação para a prática da caminhada.


The aim of this study was to examine the impact of a non supervised walking program on the physical activity level and change in behavior stages in the community. Sample comprised 114 individuals of both genders, with average age of 38.14 ± 11.41 years old. This study was divided into two periods, totalizing 33 weeks, being 16 professionally oriented weeks and 17 weeks without supervision of the walking group. The analysis methods used were: descriptive analysis, non-parametric analysis of variance, delta percentage, chi-square test and univariate regression, with the adopted level of significance p< 0.05. The subjects were classified as insufficiently active (68%) and as sufficiently active (only 32%). After an orientation period, 43% of individuals were classified as insufficiently active, and 57% as sufficiently active. After 17 weeks without supervision, the proportions were similar to the period of orientation, being significantly different from the baseline (p <0.05). A 97,14% decrease in the number of individuals classified as pre-contemplative was observed between baseline and orientation period, also an increase of 96,3% in subjects classified in the maintenance stage in the same period was noticed. Non supervised walking interventions are able to increase the level of physical activity. The knowledge obtained during the supervision and the development of the ability to walk probably influenced the maintenance of the physical activity level in the short and medium term after the suspension of the orientation of the walking program.


Asunto(s)
Humanos , Masculino , Femenino , Adulto , Conducta , Actividad Motora , Caminata , Planificación Social , Estilo de Vida
20.
Rev. bras. ciênc. mov ; 14(3): 7-14, 2006. tab
Artículo en Portugués | LILACS | ID: lil-524624

RESUMEN

Os objetivos do estudo foram: verificar a associação entre os testes de agilidade “Shuttle Run” (SR) e “Shuttle Run” com bola (SRB) em jogadores de futebol em diferentes categorias, posição de jogo e estágios maturacionais; e determinar a associação entre o SRB e o desempenho do passe em partida oficial. Metodologia: 96 meninos, entre 10 e 17 anos, divididos em quatro categorias: pré-mirim, mirim, infantil e juvenil, considerando ainda a posição de jogo (laterais, zagueiros, volantes, meio-atacantes e atacantes); e estágios maturacionais: pré púbere, púbere e pós púbere. Foram analisadas as variáveis: peso, estatura, maturação sexual, agilidade (SR) e agilidade específica para jogadores de futebol (SRB). Foi determinada a correlação entre a agilidade dos praticantes e o desempenho do passe na partida, mediante a avaliação de 4 jogos (2 vitórias e 2 derrotas). Resultados: Quandoanalisados todos os jogadores, encontramos uma correlação moderada significativa (r = 0,66) entre os testes SR e SRB. As associações encontradas em todas as posições variaram de baixa (r= 0,34) a alta (r = 0,81), evidenciando correlação alta e significativa nos zagueiros (r= 0,81), moderada significativa nos atacantes (r= 0,51), laterais (r= 0,58) e volantes (r= 0,68). Correlação baixa não significativa foi encontrada somente entre os meio-atacantes (r= 0,51). Foi evidenciada alta correlação entre SR e SRB na categoria juvenil (r= 0,78) e moderada nas categoriasinfantil (r= 0,63), mirim (r= 0,64) e pré-mirim (r=0,64), todos os valores foram significativos. Nos estágios de maturação sexual foram evidenciadas correlações moderadas significativas entre os testes, pré-púbere (r= 0,56), púbere (r= 0,61) e pós-púbere (r= 0,63). Os resultados encontrados mostraram correlação negativa, moderada e significativa (r= -0,40) entre o teste SRB e o desempenho do passe bom com coeficiente de determinação de 16%.


The objectives of this study were: a) to verify the association between the agility tests Shuttle Run (SR), and Shuttle Run with ball (SRB) in soccer players in different categories, match position, as well as maturational stages. b) Also to determine the association between SRB and pass performance in official matches. Methodology: 96 male students from a soccer school, ranging between 10 to 17 years old were divided into four categories: “Pre-Mirim” (range 10-11 years old) , “Mirim” (range 12-13 years old), “Infantil” (14 -15 years old) and “Juvenil” (16-17 years old), taking into consideration their position in the match(side back, defender, full back, midfielder, forward); and maturational stages: pre pubertal, pubertal, and post pubertal. Weight, height, sexual maturation and agility were analyzed, being agility measured through SR test and specific agility for soccer players through SRB test. The correlation between players’ agility and pass performance in the game was determined, being the passes analyzed through Scout obtained in four matches (2 victories and 2 defeats). Results: When all the players were analyzed altogether, a significant moderate correlation (r= 0.66) between SR and SRB was found. The associations found in all positions varied from high (r= 0.81) to low (0.34), highlighting significant and high correlation in the defenders, but a significant and moderate correlation in the forwards, side backs and full backs, and a non-significant low correlation was only found in the midfielders. It was evidenced a high correlation between SR and SRB in “Juvenil” category (16-17), and moderate in the other three ones – “Infantil” (14-15), “Mirim” (12 -13), “Pre Mirim” (10-11), being all of them significant. In the sexual maturation stages significant moderate evidences between the tests were evidenced: pre pubertal (r= 0.56), pubertal (r= 0.61) and post pubertal (r= 0.63).


Asunto(s)
Humanos , Masculino , Adolescente , Educación y Entrenamiento Físico/métodos , Destreza Motora , Aptitud Física , Fútbol , Prueba de Esfuerzo/métodos
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