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Conventional liquid treatments for large-scale, low-level radioactive wastewater, such as ion exchange and waste solidification, face challenges due to the large amounts of secondary waste and high disposal costs. A new large-scale decontamination method is proposed that uses kapok fiber composites for rapid radionuclide adsorption and high volume reduction to minimize secondary waste. The composite consists of natural zeolite and kapok holocellulose, which has high water-soaking ability and low-temperature pyrolysis. The kapok composites, fabricated using a commercial wet-laid nonwoven manufacturing process, absorbs 99% of low-level radioactive cesium in 20 min, reducing the volume by 98% and the weight by 47% at 300 °C. The low-temperature pyrolysis process below 300 °C prevents cesium desorption and gasification by avoiding zeolite destruction. The mass-producible kapok composites can be used for adsorbing various radionuclides in large-scale wastewater by attaching specific adsorbents for target isotopes to the composites.
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Residuos Radiactivos , Zeolitas , Aguas Residuales , Cesio , Radioisótopos , Adsorción , Residuos Radiactivos/prevención & controlRESUMEN
OBJECTIVES: The clinical outcomes and prevalence of adverse events associated with biliary biodegradable stents (BS) can differ according to degradation process and time. The aim of this study was to observe the degradation process and time of different BS prototypes, and to evaluate sequential changes in their mechanical properties. METHODS: Using an in vitro bile flow phantom model, we compared degradation time, radial force changes, and morphologic changes among four different BS prototypes: polydioxanone (PDO) BS, polyglycolide (PGA) BS, polydioxanone/poly-l-lactic acid (PDO/PLLA) sheath-core BS, and polydioxaone/magnesium (PDO/Mg) sheath-core BS. Using an in vivo swine bile duct dilation model, we performed a direct peroral cholangioscopy (DPOC) examination to observe the biodegradation process and related adverse events at regular intervals. RESULTS: In the bile flow phantom model, the PGA BS and PDO/Mg BS prototypes showed rapid radial force reduction and morphological changes and complete degradation within six weeks. PDO/PLLA BS maintained high radial force and kept their original shape for longer than the PDO BS, up to 16 weeks. A total of 24 BS were inserted into the dilated bile ducts of 12 swine. In this animal model, DPOC examination revealed that PDO BS and PDO/PLLA BS maintained their original shapes for approximately 12 weeks, but PDO BS showed a greater degree of fragmentation and induced biliary stones and bile duct obstruction. CONCLUSION: Our results showed that PDO/PLLA BS maintained their original shape and radial force for a relatively long time and minimized adverse events.
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Procedimientos Quirúrgicos del Sistema Biliar , Colestasis , Animales , Conductos Biliares/cirugía , Colangiopancreatografia Retrógrada Endoscópica , Colestasis/etiología , Colestasis/cirugía , Dilatación , Stents , PorcinosRESUMEN
Recently, a simple surface modification treatment of titanium (Ti) was developed to produce nano-and micro-scale features on the surfaces via simple immersion in an oxidative aqueous solution (30% hydrogen peroxide/5% sodium bicarbonate). However, this treatment method of Ti surfaces requires a relatively long immersion time (4 h) in the oxidative solution. In this study, we investigated whether an increase in the temperature of the oxidative etching solution can shorten the immersion time of Ti effectively. Polished grade 5 dental Ti (Ti-6Al-4V) discs were immersed in the oxidative aqueous solution either for 30 or 60 min. The temperature of the etching solution was maintained at 25 (similar to room temperature), 35, or 45 °C during etching. The etched surfaces were studied in terms of micro- and nano-structures, surface roughness, and wettability (surface energy). The increase in the temperature of the solution accelerated the etching effect of Ti and created both micro- and nano-structures on the surfaces more effectively. In particular, immersion for 60 min at the solution temperature of 35 °C significantly increased the surface roughness and wettability, although the etching effect was enhanced further at the solution temperature of 45 °C.
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In this in vitro study, nystatin-alginate microparticles were successfully fabricated to control the release of nystatin from a commercial dental tissue conditioner. These nystatin-alginate microparticles were spherical and had a slightly rough surface. The microparticles incorporated into the tissue conditioner were distributed homogeneously throughout the tissue conditioner matrix. The incorporation of the microparticles did not deteriorate the mechanical properties of the original material. The agar diffusion test results showed that the tissue conditioner containing the microparticles had a good antifungal effect against Candida albicans. The nystatin-alginate microparticles efficiently controlled the release of nystatin from the tissue conditioner matrix over the experimental period of 14 days. Moreover, the nystatin-alginate microparticles incorporated in the tissue conditioner showed effective antifungal function even at lower concentrations of nystatin. The current study suggests that the tissue conditioner containing the nystatin-alginate microparticle carrier system has potential as an effective antifungal material.
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Alginatos/farmacología , Antifúngicos/farmacología , Nistatina/farmacología , Acondicionamiento de Tejidos Dentales , Candida albicans/efectos de los fármacos , Alineadores Dentales , Ácido Glucurónico/farmacología , Ácidos Hexurónicos/farmacologíaRESUMEN
The selective laser melting (SLM) process parameters, which directly determine the melting behavior of the metallic powders, greatly affect the nanostructure and surface roughness of the resulting 3D object. This study investigated the effect of various laser process parameters (laser power, scan rate, and scan line spacing) on the surface roughness of a nickel-chromium (Ni-Cr) alloy that was three-dimensionally (3D) constructed using SLM. Single-line formation tests were used to determine the optimal laser power of 200 W and scan rate of 98.8 mm/s, which resulted in beads with an optimal profile. In the subsequent multi-layer formation tests, the 3D object with the smoothest surface (Ra = 1.3 µm) was fabricated at a scan line spacing of 60 µm (overlap ratio = 73%). Narrow scan line spacing (and thus large overlap ratios) was preferred over wide scan line spacing to reduce the surface roughness of the 3D body. The findings of this study suggest that the laser power, scan rate, and scan line spacing are the key factors that control the surface quality of Ni-Cr alloys produced by SLM.
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Self-assembled monolayers of thiols have been used to link a range of materials to planar gold surfaces or gold nanoparticles in nanoscience and nanotechnology. Novel mercapto silane systems are a promising alternative to dental noble metal alloys for enhanced resin bonding durability Goldbased alloys for full-cast restorations contain various base metal elements, which may bond to acidic functional monomers chemically, in addition to noble metal elements. This study examined how the additional incorporation of a phosphate monomer (di-2-hydroxyethyl methacryl hydrogenphosphate, DHP) into novel mercapto silane primer systems affected the resin bond strength to a type IV gold alloy pretreated with the primers. One of three commercial primers (Alloy Primer and M. L. Primer) and three experimental primer systems ((1) blend of γ-mercaptopropyltrimethoxysilane (SPS) and γ-methacryloxypropyltrimethoxysilane (MPS) (both 1.0 wt%), (2) 1.0 wt% DHP-containing primer, and (3) blend of SPS, MPS, and DHP (each 1.0 wt%)) was applied to the alloy surfaces after sandblasting. Resin cylinders (diameter: 2.38 mm) were bonded to the surfaces and light-cured. All bonded specimens were stored in water at 37 °C for 24 h and then half of them additionally water immersed for 7 days (37 °C) and thermocycled 10,000 times before the shear bond strength test (n = 10). The mercapto silane systems (SPS + MPS) were found to show superior resin bonding durability to the commercial primers and the only DHP-containing primer, regardless of additional incorporation of the phosphate monomer.
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PURPOSE: To develop a methodology for the synthesis of ß-tricalcium phosphate (ß-TCP, Ca3(PO4)2) from the shell of Haliotis sp. (abalone shell) and to verify its characterization and biocompatibility. MATERIALS AND METHODS: Calcium oxide (CaO) was synthesized from abalone shell by sintering and was suspended in distilled water to prepare calcium hydroxide (Ca(OH)2). For the synthesis of calcium carbonate (CaCO3), carbon dioxide was used to infuse Ca(OH)2 at pH 7.4. CaCO3 was reacted with phosphoric acid at pH 6.0 to obtain dicalcium phosphate (CaHPO4). Subsequently, ß-TCP was synthesized by a chemical reaction between CaHPO4 and CaO at 950°C to 1100°C for 3 hours. Fourier transform infrared spectroscopy (FT-IR) and x-ray diffraction (XRD) was performed to verify the physiochemical characteristics of the composite synthesized from abalone shell. RESULTS: FT-IR and XRD results showed that ß-TCP was successfully synthesized from abalone shell. The synthesized ß-TCP did not affect cell viability of either normal human oral keratinocytes or osteoblastic MG-63 cells. These data indicate that ß-TCP synthesized from abalone shell is biologically safe. CONCLUSIONS: ß-TCP (Ca3(PO4)2) synthesized from abalone shell can be used as a potential source of bone grafting material.
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Exoesqueleto/química , Materiales Biocompatibles/síntesis química , Fosfatos de Calcio/síntesis química , Gastrópodos/química , Animales , Espectroscopía Infrarroja por Transformada de Fourier , Difracción de Rayos XRESUMEN
A functional dental restorative system with antimicrobial properties was developed using zeolite (ZE) nanoparticles (NPs) as a drug delivery carrier. ZE NPs loaded with chlorhexidine (CHX) were prepared using the ionic immobilization method. The resulting CHX-loaded ZE NPs were then incorporated into commercial dental glass ionomer cement (GIC). The average size of the CHX-loaded ZE NPs was about 100 to 200 nm, and the NPs were dispersed homogeneously in the GIC. The in vitro release profile of encapsulated GIC containing CHX showed an early release burst of approximately 30% of the total CHX by day 7, whereas GIC containing CHX-loaded ZE NPs showed a sustained release of CHX without the early release burst in a 4-week immersion study. The agar diffusion test results showed that the GIC incorporated with CHX-loaded ZE NPs showed a larger growth inhibition zone of Streptococcus mutans than GIC alone, indicating that this innovative delivery platform potently imparted antimicrobial activity to the GIC. Moreover, these findings suggest that a range of antimicrobial drugs that inhibit the growth of oral bacteria can be incorporated efficiently into dental GIC using CHX-loaded ZE NPs.
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Antiinfecciosos , Clorhexidina , Cementos de Ionómero Vítreo , Nanopartículas/química , Streptococcus mutans/crecimiento & desarrollo , Zeolitas , Antiinfecciosos/química , Antiinfecciosos/farmacocinética , Antiinfecciosos/farmacología , Clorhexidina/química , Clorhexidina/farmacocinética , Clorhexidina/farmacología , Preparaciones de Acción Retardada/química , Preparaciones de Acción Retardada/farmacocinética , Preparaciones de Acción Retardada/farmacología , Cementos de Ionómero Vítreo/química , Cementos de Ionómero Vítreo/farmacocinética , Cementos de Ionómero Vítreo/farmacología , Zeolitas/química , Zeolitas/farmacocinética , Zeolitas/farmacologíaRESUMEN
Novel porous biphasic calcium phosphate (BCP) granules incorporated with drug-releasing poly(lactic-co-glycolic acid) (PLGA) nanoparticles were developed as a drug delivery platform for bone regeneration. The charge interaction between the BCP and PLGA nanoparticle surfaces was manipulated to create this combination system. Spherical BCP granules with open micro-channels and PLGA nanoparticles loaded with dexamethasone (DEX) as a model drug were fabricated using a liquid nitrogen method and standard emulsion method, respectively. Polyethyleneimine was coated on the DEX-loaded PLGA nanoparticle surfaces, resulting in a net positively charged surface. Such modified nanoparticles were immobilized physically on the negatively charged BCP granule surfaces. An in vitro evaluation of MG 63 cells cultured for 1 and 2 weeks on the BCP granules containing DEX-loaded PLGA nanoparticles showed greater cell proliferation, differentiation, and a more extensively connected-tissue network than those cultured on the BCP granules alone. This innovative platform for bioactive molecule delivery more potently induced osteogenesis in vitro, which might be exploited in implantable bioceramic bone graft materials for stem cell therapy or improved in vivo performance.
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Materiales Biocompatibles/química , Fosfatos de Calcio/química , Portadores de Fármacos/química , Nanopartículas/química , Materiales Biocompatibles/farmacología , Fosfatos de Calcio/farmacología , Diferenciación Celular/efectos de los fármacos , Línea Celular Tumoral , Supervivencia Celular/efectos de los fármacos , Humanos , Ácido Láctico , Microscopía Fluorescente , Osteogénesis , Tamaño de la Partícula , Ácido Poliglicólico , Copolímero de Ácido Poliláctico-Ácido Poliglicólico , Porosidad , Ingeniería de TejidosRESUMEN
Plasma treatments are becoming a popular method for modifying the characteristics of a range of substrate surfaces. Atmospheric pressure plasma is cost-efficient, safe and simple compared to high-pressure plasma. This study examined the effects of atmospheric pressure plasma to a titanium (Ti) surface on osteoblast-like cell (osteoblast) spreading and cellular networks. The characteristics of the Ti surface before and after the atmospheric plasma treatment were analyzed by X-ray photoemission spectroscopy (XPS), scanning electron microscopy (SEM), contact angle measurements, and an optical 3D profiling system. The morphology of osteoblasts attached to the Ti surfaces was observed by SEM and confocal laser scanning microscopy. The atmospheric pressure plasma made the Ti surfaces more hydrophilic. The osteoblasts that adhered to the untreated surface were round and spherical, whereas the cells covered a larger surface area on the plasma-treated surface. The plasma-treated Ti surface showed enhanced cell spreading and migration with more developed cellular networks. In conclusion, an atmospheric plasma treatment is a potential surface modifying method that can enhance the initial the cell affinity at the early stages in vitro.
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Proliferación Celular/efectos de los fármacos , Forma de la Célula/efectos de los fármacos , Osteoblastos/efectos de los fármacos , Gases em Plasma/farmacología , Titanio/química , Animales , Presión Atmosférica , Línea Celular , Ratones , Gases em Plasma/química , Propiedades de SuperficieRESUMEN
Establishing a strong resin bond to dental zirconia ceramic remains difficult. Previous studies have shown that the conventional application of silane does not work well with zirconia. This paper reports that a silane pre-treatment of dental zirconia ceramic combined with subsequent heat treatment has potential as an adhesive cementation protocol for improving zirconia-resin bonding. Among the various concentrations (0.1 to 16 vol%) of experimental γ-methacryloxypropyltrimethoxysilane (γ-MPTS) primers assessed, the 1% solution was found to be the most effective in terms of the shear bond strength of the resin cement to dental zirconia ceramic. A high shear bond strength (approx. 30 MPa) was obtained when zirconia specimens were pre-treated with this primer and then heat-treated in a furnace for 60 min at 150 degrees C. Heat treatment appeared to remove the hydrophilic constituents from the silane film formed on the zirconia ceramic surface and accelerate the condensation reactions between the silanol groups of the hydrolyzed silane molecules at the zirconia/resin interface, finally making a more desirable surface for bonding with resin. This estimation was supported by Fourier transform infrared spectroscopy of the silanes prepared in this study.
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Cerámica/química , Metacrilatos/química , Nanopartículas/química , Silanos/química , Circonio/química , Recubrimiento Dental Adhesivo , Calor , Resinas Sintéticas , Resistencia al Corte , Propiedades de SuperficieRESUMEN
Self-assembled monolayers of thiols (RSH), which are key elements in nanoscience and nanotechnology, have been used to link a range of materials to planar gold surfaces or gold nanoparticles. In this study, the adhesive performance of mercapto silane systems to dental noble metal alloys was evaluated in vitro and compared with that of commercial dental primers. Dental gold-palladium-platinum (Au-Pd-Pt), gold-palladium-silver (Au-Pd-Ag), and palladium-silver (Pd-Ag) alloys were used as the bonding substrates after air-abrasion (sandblasting). One of the following primers was applied to each alloy: (1) no primer treatment (control), (2) three commer- cial primers: V-Primer, Metal Primer II, and M.L. Primer, and (3) two experimental silane primer systems: 2-step application with 3-mercaptopropyltrimethoxysilane (SPS) (1.0 wt%) and then 3-methacryloxypropyltrimethoxysilane (MPS) (1.0 wt%), and a silane blend consisting of SPS and MPS (both 1.0 wt%). Composite resin cylinders with a diameter of 2.38 mm were bonded to the surfaces and irradiated for 40 sec using a curing light. After storage in water at 37 °C for 24 h, all the bonded specimens were thermocycled 5000 times before the shear bond strength test. Regardless of the alloy type, the mercapto silane systems (both the 2-step and blend systems) consistently showed superior bonding performance than the commercial primers. Contact angle analysis of the primed surfaces indicated that higher resin bond strengths were produced on more hydrophilic alloy surfaces. These novel mercapto silane systems are a promising alternative for improving resin bonding to dental noble metal alloys.
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Aleaciones Dentales/química , Resinas Sintéticas/química , Silanos/química , Compuestos de Sulfhidrilo/química , Oro/química , Paladio/química , Platino (Metal)/químicaRESUMEN
Plasma treatments are becoming a popular method for modifying the characteristics of a range of substrate surfaces. Atmospheric pressure plasma (APP) is cost-efficient, safe and simple compared to high-pressure plasma. This study examined the effects of a low-temperature APP treatment of a hydroxyapatite (HA)-coated Ti (HA-Ti) surface. The APP treatment made the HA-Ti surfaces more hydrophilic without changing surface morphologies. The APP-treated HA-Ti (APP-HA-Ti) surface showed enhanced cell spreading, cell proliferation, and alkaline phosphatase (ALP) levels with more developed cellular networks, and the formed extracellular matrix (ECM) was fused perfectly with the HA substrate than that on the HA-Ti surface. In conclusion, an APP treatment is a potential surface-modifying method that can enhance the cell affinity at the early stages in vitro.
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Durapatita/química , Nanopartículas/química , Osteoblastos/citología , Gases em Plasma/química , Prótesis e Implantes , Titanio/química , Adsorción , Animales , Presión Atmosférica , Sustitutos de Huesos/síntesis química , Adhesión Celular/fisiología , Línea Celular , Proliferación Celular/fisiología , Supervivencia Celular/fisiología , Materiales Biocompatibles Revestidos/síntesis química , Ensayo de Materiales , Ratones , Nanopartículas/ultraestructura , Osteoblastos/fisiología , Tamaño de la Partícula , Propiedades de SuperficieRESUMEN
We developed a simple and highly efficient method for delivery from titanium (Ti) surfaces using albumin nanoparticle carriers. A Ti disc with a resorbable blasting media surface was used as a metal implant with a localized drug delivery structure. Human serum albumin (HSA) nanoparticles loaded with chlorhexidine (CHX) diacetate salt hydrate as the model drug were fabricated using a desolvation technique. The CHX-loaded HSA nanoparticles produced were cross linked with glutaraldehyde (GA). The nanoparticles were pre-coated with positively-charged polyethylenimine (PEI) molecules and then immobilized via electrical interactions on the negatively charged Ti disc surface. Our results suggested that the PEI-coated HSA nanoparticles loaded with CHX (PEI-CHX-HSA) were incorporated successfully and well-dispersed on the Ti disc surfaces. The agar diffusion test on the Ti surface treated with PEI-CHX-HSA nanoparticles showed a larger growth inhibition zone of Streptococcus mutans versus the control Ti surface, suggesting that this innovative delivery platform imparts potent antibacterial activity to the Ti surface. Thus, CHX, which inhibits the growth of oral bacteria, can be efficiently incorporated onto Ti surfaces by using HSA nanoparticles.
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Antibacterianos/administración & dosificación , Antibacterianos/química , Nanopartículas/química , Albúmina Sérica/química , Titanio/química , Antibacterianos/farmacocinética , Clorhexidina/administración & dosificación , Clorhexidina/química , Clorhexidina/farmacocinética , Sistemas de Liberación de Medicamentos/métodos , Humanos , Nanopartículas/administración & dosificación , Albúmina Sérica/administración & dosificación , Streptococcus mutans/efectos de los fármacos , Propiedades de SuperficieRESUMEN
OBJECTIVE: This study investigated the effect of different drying methods of dentin surface on the bonding efficacy of self-adhesive resin cements (SRCs). MATERIALS AND METHODS: Three SRCs (RelyX U200, RU; Maxcem Elite, ME; and BisCem, BC) and one resin-modified glass ionomer cement (RelyX Luting 2, RL) were used. The characteristics of the materials were evaluated using thermogravimetric analysis and surface roughness and contact angle measurements. Human dentin surfaces were finished with 600-grit silicon carbide paper and assigned to three groups according to these drying methods: ethanol dehydration, drying by waiting for 10 s after blot-drying and blot-drying. The four cements were used for luting composite overlays to the dried dentin. After 24 h storage at 37°C and 100% relative humidity, stick-shaped specimens with a cross-sectional area of 0.8 mm(2) were prepared and stressed to failure in tension at a crosshead speed of 0.5 mm/min (n = 27). Failure modes of fractured specimens were assessed by optical and scanning electron microscopy. RESULTS: RL was the most hydrophilic, followed by BC and ME and then RU. All the luting cements luted to ethanol-dehydrated dentin showed zero bond strengths. For the three SRCs, drying by waiting produced higher microtensile bond strengths than blot-drying. RU showed the best bonding performance in the above two dentin conditions. RL showed significantly higher bond strength in blot-drying condition than in drying-by-waiting (p < 0.001). CONCLUSIONS: This study suggests that dentin surface moisture has a crucial effect on the bond strength of SRCs.
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Recubrimiento Dental Adhesivo , Dentina/ultraestructura , Cementos de Resina/química , Resinas Compuestas/química , Materiales Dentales/química , Análisis del Estrés Dental/instrumentación , Recubrimientos Dentinarios/química , Desecación , Etanol/química , Cementos de Ionómero Vítreo/química , Humanos , Humedad , Ensayo de Materiales , Microscopía Electrónica de Rastreo , Solventes/química , Espectroscopía Infrarroja por Transformada de Fourier/métodos , Estrés Mecánico , Propiedades de Superficie , Temperatura , Resistencia a la Tracción , Factores de Tiempo , HumectabilidadRESUMEN
Endoscopic biliary stent insertion has been widely used for the treatment of benign biliary stricture (BBS). Thus, the development of stent materials in the perspectives of structure, mechanical properties, and biocompatibility has been also studied. However, conventional metal and plastic stents have several disadvantages, such as repeated procedures to remove or exchange them, dislodgment, restenosis, biocompatibility, and poor mechanical properties. Sustainable effectiveness, attenuation and prevention of fibrosis, and biocompatibility are key factors for the clinical application of stents to BBS treatment. In addition, loading drugs could show synergistic effects with stents' own performance. We developed a dexamethasone-eluting biodegradable stent (DBS) consisting of a sheath/core structure with outstanding mechanical properties and sustained release of dexamethasone, which maintained its functions in a BBS duct over 12 weeks in a swine model. The insertion of our DBS not only expanded BBS areas but also healed secondary ulcers as a result of the attenuation of fibrosis. After 16 weeks from the insertion, BBS areas were totally improved, and the DBS was degraded and thoroughly disappeared without re-intervention for stent removal. Our DBS would be an effective clinical tool for non-vascular diseases. STATEMENT OF SIGNIFICANCE: This study describes the insertion of a drug-eluting biodegradable stent (DBS) into the bile duct. The sheath/core structure of DBS confers substantial durability and a sustained drug release profile. Drug released from the DBS exhibited anti-fibrotic effects without inflammatory responses in both in vitro and in vivo experiments. The DBS maintained its function over 12 weeks after insertion into the common bile duct, expanding benign biliary stricture (BBS) and reducing inflammation to heal secondary ulcers in a swine BBS model. After 16 weeks from the DBS insertion, the DBS thoroughly disappeared without re-intervention for stent removal, resulting in totally improved BBS areas. Our findings not only spotlight the understanding of the sheath/core structure of the biodegradable stent, but also pave the way for the further application for non-vascular diseases.
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Colestasis , Úlcera , Animales , Porcinos , Constricción Patológica , Stents , Colestasis/terapia , Fibrosis , Dexametasona/farmacologíaRESUMEN
Barrier membranes for guided bone regeneration (GBR) were prepared by a solvent casting method using solutions of poly(L-lactic acid) (PLLA) and chitosan. PLLA and PLLA/chitosan membranes were treated with ammonia gas plasma. PLLA/chitosan membranes were successfully fabricated, and the surface of the PLLA/chitosan membrane was clearly modified by NH3 plasma treatment according to attenuated total reflectance (ATR-FTIR), X-ray photoelectron spectroscopy (XPS) and scanning electron microscopy (SEM) analyses. Additionally, water contact angle testing indicated that the hydrophilicity of these membranes was significantly increased. MG-63 cells were cultured on each type of membrane, and cell viability was examined using an MTT assay. After one week of culturing, MG-63 cells were more abundant on PLLA/chitosan membranes than on PLLA membranes. The cell viability of PLLA/chitosan membranes with plasma treatment was significantly higher than that of PLLA membranes. These results suggest that this plasma-treated membrane is suitable for GBR and is a promising source of bioactive membrane material for bone regeneration.
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Amoníaco/química , Regeneración Ósea , Adhesión Celular , Regeneración Tisular Dirigida/instrumentación , Ácido Láctico/química , Membranas Artificiales , Osteoblastos/citología , Polímeros/química , Línea Celular , Humanos , Microscopía Electrónica de Rastreo , Espectroscopía de Fotoelectrones , Poliésteres , Espectroscopía Infrarroja por Transformada de FourierRESUMEN
A novel barrier membrane composed of poly(lactic-co-glycolic acid) particles loaded with dexamethasone (DEX) as a bioactive molecule was produced via a modified nanoprecipitation method without any mixing. The particle membranes had a bilayer structure: one side was smooth and had a compact surface that was connected to larger particles, while the opposite side was rough, porous and connected to smaller particles. Additionally, a cross-section of the particle membrane had a porous structure with nano and micro sized irregular pores. Process optimization revealed that NaCl concentration in the water phase, with acetone as solvent and water as a non-solvent, played critical roles in determining the properties of the particle membranes, such as DEX encapsulation efficiency, thickness and surface morphologies of the particle membranes. A novel barrier membrane containing DEX using polymer particle drug capture technology has been successfully developed.
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Antiinflamatorios/administración & dosificación , Regeneración Ósea/efectos de los fármacos , Dexametasona/administración & dosificación , Portadores de Fármacos/administración & dosificación , Ácido Láctico/administración & dosificación , Nanopartículas , Ácido Poliglicólico/administración & dosificación , Portadores de Fármacos/química , Sistemas de Liberación de Medicamentos , Ácido Láctico/química , Ácido Poliglicólico/química , Copolímero de Ácido Poliláctico-Ácido PoliglicólicoRESUMEN
PURPOSE: The current drain tubes for preventing surgically biliary anastomotic stricture are not naturally and easily removed. If a drain tube using biodegradable material is easily available and the degradation time of the tube is well controlled, surgical anastomotic stricture and fibrosis could be prevented. The aim of this animal study was to evaluate the preventive effect of novel biodegradable stents (BS) on biliary stricture and fibrosis after duct-to-duct (DD) biliary anastomosis. METHODS: Ten mini-pigs were allocated to the control group (n = 5) and or the stent group (n = 5). The common bile duct was exposed through surgical laparotomy and then resected transversely. In the stent group, a 4-mm or 6-mm polydioxanone/magnesium sheath-core BS was inserted according to the width of the bile duct, followed by DD biliary anastomosis. In the control group, DD biliary anastomosis was performed without BS insertion. RESULTS: In the stent group, stents were observed without deformity for up to 4 weeks in all animals. Eight weeks later, histopathologic examination revealed that the common bile duct of the anastomosis site was relatively narrower in circumference in the control group compared to the stent group. The degree of fibrosis in the control group was more marked than in the stent group (3.84 mm vs. 0.68 mm, respectively; P < 0.05). CONCLUSION: Our study showed that novel BS maintained their original shape and radial force for an adequate time and then disappeared without adverse events. The BS could prevent postoperative complications and strictures after DD biliary anastomosis.
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This study was performed to identify the calcium phosphate minerals, chemical element and Ca/P ratio and to examine the surface structure of autogenous tooth bone grafting material (AutoBT) which recently developed and applied clinically as a bone graft materials. The analytical results showed that AutoBT is composed of low-crystalline hydroxyapatite (HA) and possibly other calcium phosphate minerals, which is similar to the minerals of human bone tissues. And the dental crown portion was composed of high-crystalline calcium phosphate minerals (mainly HA) with higher Ca/P ratio while the root portion was mainly composed of low-crystalline calcium phosphates with relatively low Ca/P ratio.