MicroRNA-143-3p inhibits colorectal cancer metastases by targeting ITGA6 and ASAP3.

MicroRNAs, which regulate mRNAs, operate through a variety of signaling pathways to participate in the development of colorectal cancer (CRC). In this study, we found that microRNA (miR)-143-3p expression was significantly lower in both CRC and liver metastatic CRC tissues from liver compared with normal colonic tissues. Functional assays showed that miR-143-3p inhibited CRC cell invasion and migration in vitro. Using a bioinformatics approach, we identified miR-143-3p target mRNAs. Among the candidate targets, only the expression of integrin alpha 6 (ITGA6) and ArfGAP with the SH3 domain and ankyrin repeat and PH domain 3 (ASAP3) were significantly reduced by miR-143-3p mimics as examined by western blot, and the metastasis potential of CRC cells was attenuated by endogenous ITGA6 and ASAP3 knockdown, determined by migration and invasion assays. Both ITGA6 and ASAP3 were upregulated in CRC tissues compared to normal tissues. Analysis of the relationship between clinicopathological features and ITGA6/ASAP3 protein expression in 200 patients with CRC showed a significant difference in positive ITGA6 expression between the early stage (I + II) and the advanced stage (III + IV), and ASAP3 expression levels positively correlated with metastasis in the lymph nodes. These results indicate that miR-143-3p acts as an anti-oncogene by downregulating ITGA6/ASAP3 protein expression and could offer new insight into potential therapeutic targets for CRC.

Leakage Evaluation by Virtual Entropy Generation (VEG) Method.

Leakage through microscale or nanoscale cracks is usually hard to observe, difficult to control, and causes significant economic loss. In the present research, the leakage in a pipe was evaluated by the virtual entropy generation (VEG) method. In virtual entropy generation method, the "measured entropy generation" is forced to follow the "experimental second law of thermodynamics". Taking the leakage as the source virtual entropy generation, a new pipe leakage evaluation criterion was analytically derived, which indicates that the mass leakage rate should be smaller than the pressure drop rate inside a pipe. A numerical study based on computational fluid dynamics showed the existence of an unrealistic virtual entropy generation at a high mass leakage rate. Finally, the new criterion was used in the evaluation of leakage available in the literature. These results could be useful for leakage control or industry criteria design in the future.

Mechanism of emergency phytoremediation technology based on a 3D-QSAR pharmacological model.

Introduction: The ability of transgenic plants to respond to sudden environmental pollution accidents has become viable. Nonetheless, there is a dearth of research regarding the mechanism by which transgenic plants degrade organic pollutants. Hence, this study aimed to elucidate the process of organic pollutant degradation by plants, offering theoretical support for the application of transgenic plant emergency phytoremediation technology. Methods: In this investigation, we developed a 3D-QSAR pharmacophore model to represent the collective impact of plant resistance and phytodegradation. This was achieved by employing integrated effect values following treatment with a sine function approach. Moreover, we have undertaken an inaugural exploration of the coregulatory mechanism involved in plant resistance and pollutant degradation within plants. Additionally, we applied virtual molecular modification techniques for analysis and validation, striving for a more indepth understanding of the molecular-level enhancement mechanism related to the degradation of pollutants within plant organisms. Results and discussion: The mechanism analysis results of the Hypo 1 pharmacophore model were verified, indicating that hydrophobic characteristics affect the resistance and degradation of PCBs in plants, significantly affecting the degradation effect of pollutants in plants.

Potential Environmental Risk Characteristics of PCB Transformation Products in the Environmental Medium.

The complementary construction of polychlorinated biphenyl (PCB) phytotoxicity and the biotoxicity 3D-QSAR model, combined with the constructed PCB environmental risk characterization model, was carried out to evaluate the persistent organic pollutant (POP) properties (toxicity (phytotoxicity and biotoxicity), bioconcentration, migration, and persistence) of PCBs and their corresponding transformation products (phytodegradation, microbial degradation, biometabolism, and photodegradation). The transformation path with a significant increase in environmental risks was analyzed. Some environmentally friendly PCB derivatives, exhibiting a good modification effect, and their parent molecules were selected as precursor molecules. Their transformation processes were simulated and evaluated for assessing the environmental risks. Some transformation products displayed increased environmental risks. The environmental risks of plant degradation products of the PCBs in the environmental media showed the maximum risk, indicating that the potential risks of the transformation products of the PCBs and their environmentally friendly derivatives could not be neglected. It is essential to further improve the ability of plants to degrade their transformation products. The improvement of some degradation products for environmentally friendly PCB derivatives indicates that the theoretical modification of a single environmental feature cannot completely control the potential environmental risks of molecules. In addition, this method can be used to analyze and evaluate environmentally friendly PCB derivatives to avoid and reduce the potential environmental and human health risks caused by environmentally friendly PCB derivatives.

Reliable Interlayer Based on Hybrid Nanocomposites and Carbon Nanotubes for Lithium-Sulfur Batteries.

The future energy needs have triggered research interest in finding novel energy storage systems with high energy density. Lithium-sulfur batteries are regarded as one of the most promising options for the next-generation energy storage applications because of their high theoretical energy and low cost. However, the electrochemical performances of lithium-sulfur batteries are seriously compromised by the polysulfide (LiPS) shuttling and the insulating nature of sulfur. To overcome these issues, novel CoNi1/3Fe2O4 (CNFO) nanoparticles uniformly covered on the carbon nanotubes are now reported as an efficient functional interlayer. Benefiting from the sufficient sulfiphilic sites of the CNFO for chemically bonding with LiPSs, as well as the conductive interconnected skeleton of carbon nanotubes, this composite material showed great enhancement on the rate capability and cycle stability of Li-S batteries. The Li-S battery using this interlayer exhibited a high initial capacity of 897 mA h g-1 and a low capacity decay of 0.063% per cycle within 250 cycles at 2 C. Meanwhile, an reversible specific capacity of 869 mA h g-1 (at 0.5 C) with high Coulombic efficiency could be obtained over 100 cycles at an elevated temperature (60 °C). We speculated that the chemical adsorption of CNFO for polysulfide-anchoring is extremely critical for the performances of Li-S batteries under high temperature.

Fabrication of Slippery Lubricant-Infused Porous Surface with High Underwater Transparency for the Control of Marine Biofouling.

Marine optical instruments are bearing serious biofouling problem, which affects the accuracy of data collected. To solve the biofouling problem of marine optical instruments, a novel instance of slippery lubricant-infused porous surface (SLIPS) with high underwater-transparency was designed over glass substrate via infusing lubricant into its porous microstructure fabricated with hydrothermal method. The advantage of SLIPS as antibiofouling strategy to marine optical instruments was proven by comparing its underwater optical and antibiofouling performances with three kinds of samples (hydrophilic glass sample, textured hydrophilic glass sample, and superhydrophobic glass sample). The modification of SLIPS enhances the underwater-transparency of glass sample within the wavelength of 500-800 nm, for the infusion of lubricant with lower refractive index than glass substrate. In contrast with hydrophilic surface, textured hydrophilic surface and superhydrophobic surface, SLIPS can significantly inhibit bacterial and algal settlements, thereby maintaining high underwater-transparency in both dynamic and static seawater. The inhibition of bacterial and algal settlements over SLIPS results from its liquid-like property. The contact angle hysteresis of water over SLIPS increases with immersion time in seawater under different conditions (static, dynamic, and vibration conditions). Both dynamic and vibration conditions accelerate the failure of SLIPS exposed in seawater. This research provides valuable information for solving biofouling problem of marine optical instruments with SLIPS.

Fabrication of Slippery Lubricant-Infused Porous Surface for Inhibition of Microbially Influenced Corrosion.

Microbially influenced corrosion (MIC) accelerates the failure of metal in a marine environment. In this research, slippery lubricant-infused porous surface (SLIPS) was designed on aluminum, and its great potential for inhibiting MIC induced by sulfate-reducing bacteria (SRB) was demonstrated in a simulated marine environment. The inhibition mechanism of SLIPS to MIC was proposed based on its effective roles in the suppression of SRB settlement and isolation effect to corrosive metabolites. The liquid-like property is demonstrated to be the major contributor to the suppression effect of SLIPS to SRB settlement. The effects of environmental factors (static and dynamic conditions) and lubricant type to SRB settlement over SLIPS were also investigated. It was indicated that the as-fabricated SLIPS can inhibit the SRB settlement in both static and dynamic marine conditions, and lubricant type presents a negligible effect on the SRB settlement. These results will provide a series of foundational data for the future practical application of SLIPS in the marine environment, and also a lubricant selecting instruction to construct SLIPS for MIC control.