RESUMEN
The synthesis of a novel carboxylate-type organic linker-based luminescent MOF (Zn(H2L) (L1)) (named PUC2) (H2L = 2-aminoterephtalic acid, L1 = 1-(3-aminopropyl) imidazole) is reported by the solvothermal method and comprehensively characterized using single-crystal XRD, PXRD, FTIR, TGA, XPS, FESEM, HRTEM, and BET. PUC2 selectively reacts with nitric oxide (âªNO) with a detection limit of 0.08 µM, and a quenching constant (0.5 × 104 M-1) indicating a strong interaction with âªNO. PUC2 sensitivity remains unaffected by cellular proteins or biologically relevant metals (Cu2+/ Fe3+/Mg2+/ Na+/K+/Zn2+), RNS/ROS, or H2S to score âªNO in living cells. Lastly, we used PUC2 to demonstrate that H2S inhibition increases âªNO production by ~ 14-30% in various living cells while exogenous H2S suppresses âªNO production, indicating that the modulation of cellular âªNO production by H2S is rather generic and not restricted to a particular cell type. In conclusion, PUC2 can successfully detect âªNO production in living cells and environmental samples with considerable potential for its application in improving the understanding of the role of âªNO in biological samples and study the inter-relationship between âªNO and H2S.