Microplastic Pollution in High Population Density Zones of Selected Rivers from Southeast Asia.

Southeast Asia (SEA) faces significant environmental challenges due to rapid population growth and economic activity. Rivers in the region are major sources of plastic waste in oceans. Concerns about their contribution have grown, but knowledge of microplastics in the area is still limited. This article compares microplastic levels in sediment and water from urban zones of three major rivers in SEA: Chao Phraya River (Thailand), Saigon River (Vietnam), and Citarum River (Indonesia). The study reveals that in all three rivers, microplastics were found, with the highest concentrations in Chao Phraya's water (80 ± 60 items/m3) and Saigon's sediment (9167 ± 4559 items/kg). The variations in microplastic sizes and concentrations among these rivers may be attributed to environmental factors and the exposure duration of plastic to the environment. Since these rivers are important water supply sources, rigorous land-use regulations and raising public awareness are crucial to mitigate plastic and microplastic pollution.

Functional networks in parallel with cortical development associate with executive functions in children.

Children begin performing similarly to adults on tasks requiring executive functions in late childhood, a transition that is probably due to neuroanatomical fine-tuning processes, including myelination and synaptic pruning. In parallel to such structural changes in neuroanatomical organization, development of functional organization may also be associated with cognitive behaviors in children. We examined 6- to 10-year-old children's cortical thickness, functional organization, and cognitive performance. We used structural magnetic resonance imaging (MRI) to identify areas with cortical thinning, resting-state fMRI to identify functional organization in parallel to cortical development, and working memory/response inhibition tasks to assess executive functioning. We found that neuroanatomical changes in the form of cortical thinning spread over bilateral frontal, parietal, and occipital regions. These regions were engaged in 3 functional networks: sensorimotor and auditory, executive control, and default mode network. Furthermore, we found that working memory and response inhibition only associated with regional functional connectivity, but not topological organization (i.e., local and global efficiency of information transfer) of these functional networks. Interestingly, functional connections associated with "bottom-up" as opposed to "top-down" processing were more clearly related to children's performance on working memory and response inhibition, implying an important role for brain systems involved in late childhood.

Abnormalities of cortical thickness, subcortical shapes, and white matter integrity in subcortical vascular cognitive impairment.

Subcortical vascular cognitive impairment (sVCI) is caused by lacunar infarcts or extensive and/or diffuse lesions in the white matter that may disrupt the white matter circuitry connecting cortical and subcortical regions and result in the degeneration of neurons in these regions. This study used structural magnetic resonance imaging (MRI) and high angular resolution diffusion imaging (HARDI) techniques to examine cortical thickness, subcortical shapes, and white matter integrity in mild vascular cognitive impairment no dementia (VCIND Mild) and moderate-to-severe VCI (MSVCI). Our study found that compared to controls (n = 25), VCIND Mild (n = 25), and MSVCI (n = 30) showed thinner cortex predominantly in the frontal cortex. The cortex in MSVCI was thinner in the parietal and lateral temporal cortices than that in VCIND Mild. Moreover, compared to controls, VCIND Mild and MSVCI showed smaller shapes (i.e., volume reduction) in the thalamus, putamen, and globus pallidus and ventricular enlargement. Finally, compared to controls, VCIND Mild, and MSVCI showed an increased mean diffusivity in the white matter, while decreased generalized fractional anisotropy was only found in the MSVCI subjects. The major axonal bundles involved in the white matter abnormalities were mainly toward the frontal regions, including the internal capsule/corona radiata, uncinate fasciculus, and anterior section of the inferior fronto-occipital fasciculus, and were anatomically connected to the affected cortical and subcortical structures. Our findings suggest that abnormalities in cortical, subcortical, and white matter morphology in sVCI occur in anatomically connected structures, and that abnormalities progress along a similar trajectory from the mild to moderate and severe conditions.

High Prevalence of Panton-Valentine Leukocidin Among Staphylococcus aureus Causing Acute Hematogenous Bone and Joint Infections From a Tertiary Children's Hospital in Vietnam.

BACKGROUND: We aimed to investigate the clinical features, antimicrobial susceptibility and pvl gene expression in Staphylococcus aureus causing acute hematogenous bone and joint infections (BJIs) in children in Vietnam. METHODS: In this prospective study, the demographics, microbiology and clinical outcomes of pediatric patients with acute hematogenous BJIs were collected from September 2022 to September 2023. Antimicrobial susceptibility profiles were determined using VITEK2 Compact system. The pvl gene encoding the Panton-Valentine leukocidin (PVL) toxin was detected by using polymerase chain reaction. Mann-Whitney, χ 2 and Fisher test were used for statistical analysis. RESULTS: In total, 78 patients (46 boys) with S. aureus acute hematogenous BJIs were recruited at the National Children's Hospital, Hanoi, Vietnam. Of all S. aureus isolates, 84.6% were methicillin-resistant S. aureus . All S. aureus isolates were susceptible to vancomycin, ciprofloxacin and levofloxacin; 97% of methicillin-resistant S. aureus isolates was resistant to clindamycin (minimum inhibitory concentration ≥8 µg/mL). The pvl gene was detected in 83.3% of isolates, including 57 methicillin-resistant S. aureus isolates. Patients in the pvl -positive group had significantly higher C-reactive protein levels than those in the pvl -negative group ( P = 0.04). In addition, all 8 children with septic shock were infected with pvl -positive S. aureus . CONCLUSIONS: PVL is a prevalent virulence factor of S. aureus in Vietnam. Furthermore, high inflammatory parameters (C-reactive protein) may be present at the time of diagnosis in PVL positivity-related acute hematogenous BJIs. Further research is necessary to enhance our understanding of the varying correlations between virulence factors and outcomes of S. aureus BJIs.

Microplastics and heavy metals in a tropical river: Understanding spatial and seasonal trends and developing response strategies using DPSIR framework.

Microplastics (MPs) have become an increasingly popular topic in recent years due to the growing concern about their impact on human health and the environment. Rivers in Southeast Asia are the dominant source of plastics and MPs into the environment; however, research on MPs in rivers from the region is insufficient. This study aims to investigate the impacts of spatial and seasonal variations on the distribution of MPs with heavy metals in one of the top 15 rivers releasing plastics into oceans (Chao Phraya, Thailand). Findings from this study are analyzed using the Driver-Pressure-State-Impact-Response (DPSIR) framework for proposing strategies to minimize plastic and MPs in this tropical river. Spatially, most MPs were detected in the urban zone, while the lowest was in the agricultural zone. Also, MP levels in the dry season are higher than at the end but lower than at the beginning of the rainy season. MPs with fragment morphology were mainly found in the river (70-78 %). Polypropylene was found with the highest percentage (54-59 %). MPs in the river were mostly detected in the size range of 0.05-0.3 mm (36-60 %). Heavy metals were also found in all MPs collected from the river. Higher metal concentrations were detected in the agricultural and estuary zones in the rainy season. Potential responses, including regulatory and policy instruments, environmental education, and environmental cleanups, were drawn from the DPSIR framework.

Occurrence and spatial distribution of microplastic contaminated with heavy metals in a tropical river: Effect of land use and population density.

Microplastics are of concern due to their potential environmental risks. This research aims to find the effect of land use (agriculture, urban, and aquaculture) and population density on the abundance of microplastics contaminated with heavy metals in surface water and sediment of the Chao Phraya River in Thailand. Results indicated that population density is directly correlated with increased microplastic abundance. Most microplastics were found in the urban zone (water: 80 ± 38 items/m3 and sediment: 62 ± 11 items/kg) and were small fragments (0.05 to 0.3 mm). Polymer types of polypropylene and polyethylene were commonly found. Heavy metals of Cr, Cu, Cd, Pb, Zn, Ni, and Ti were detected in microplastics in water and sediment, with Cu, Pb, and Zn being the most dominant. Heavy metals in microplastics were highest in the agriculture zone. In general, the presence of metals on microplastics may potentially impact the ecosystem and human health.

Age-related vulnerabilities along the hippocampal longitudinal axis.

Evidence for an anterior-posterior gradient of age-related volume reduction along the hippocampal longitudinal axis has been reported in normal aging, but functional changes have yet to be systematically investigated. The current study applied an advanced brain mapping technique, large deformation diffeomorphic metric mapping (LDDMM), automatically delineating the hippocampus into the anterior and posterior segments based on anatomical landmarks. We studied this anterior-posterior gradient in terms of structural and functional MRI in 66 participants aged from 19 to 79 years. The results showed age-related structural volume reduction in both anterior and posterior hippocampi, with greater tendency for anterior decrease. FMRI task contrasts that robustly activated the anterior (associative/relational processing) and posterior (novelty) hippocampus independently, showed only significant reduction of activation in the anterior hippocampus as age increased. Our results revealed positive correlation between structural atrophy and functional decrease in the anterior hippocampi, regardless of task performance in normal aging. These findings suggest that anatomy and functions related to the anterior hippocampus may be more vulnerable to aging, than previously thought.

Investigation of microplastic contamination in blood cockles and green mussels from selected aquaculture farms and markets in Thailand.

Recent studies have indicated that bivalves are bioindicators for microplastic pollution since they are filter feeders and accumulate microplastics (MPs) during their feeding process. This study focused on the investigation of MPs in blood cockles (Tegillarca granosa) and green mussels (Perna viridis) from selected aquaculture farms and markets in Thailand. The abundance of MPs was identified by fluorescence microscopy with Nile Red tagging and the polymer detection by micro-Fourier Transform Infrared Spectroscopy (micro-FTIR). The number of MPs in blood cockle samples from the Khlong Dan market and the BangBo aquaculture farm was 11 ± 5 and 6 ± 1 particles/individual, respectively. For green mussel samples, the number of MPs from the Talaad Thai market and the Sriracha fisheries research station was 96 ± 19 and 11 ± 7 particles/individual, respectively. The results of the study indicate that the number of MPs in bivalve samples from the markets is much higher than in the aquaculture farms. This probably is due to the contamination of MPs in bivalves during the packaging and transportation processes. However, further studies are needed to confirm this. The predominant MPs were fragments and fibres with a size range of 0.05-0.3 mm. Most polymers were polypropylene, polyethylene, copolymer, polyester, and nylon. In brief, this study showed the contamination of MPs in bivalves cultivated for human consumption. The accumulation of MPs in these marine bivalves can lead to the biomagnification of MPs along the food chain. This can impact food safety and human health.

Severe recurrent pneumonia in children: Underlying causes and clinical profile in Vietnam.

BACKGROUND: There is still limited data on severe recurrent pneumonia in children, especially in developing countries as Vietnam. This study was conducted to identify the underlying causes and clinical profile of children with severe recurrent pneumonia admitted to the Pediatric Intensive Care Unit (PICU), National Children's Hospital. METHODS: This was a prospective and descriptive study on 110 children with severe pneumonia admitted to the PICU from November 2019 to August 2020. Data were collected to investigate the clinical profile and underlying diseases. RESULTS: Severe recurrent pneumonia accounted for 29.4%. Underlying causes were diagnosed in 91.8% of sRP children, in which the most common causes were abnormalities in respiratory, cardiovascular system and immune disorders. 74.5% of sRP children admitted to ICU had been previously intubated or ventilated, 34.5% had shock, 7.3% had multiple organ failure. Recurrent lesions on chest x-ray in the same lobe accounted for 18.2%. CONCLUSIONS: The majority of patients with severe recurrent pneumonia had an underlying disease. Comprehensive management is necessary for severe recurrent pneumonia.

Microplastics pollution with heavy metals in the aquaculture zone of the Chao Phraya River Estuary, Thailand.

Microplastics have received special attention due to their potential risks to environments. However, data on microplastics in estuaries of the Southeast Asian region is still insufficient. This study investigated the abundance and characteristics of microplastics in the sediment and surface water of the Chao Phraya River Estuary, Thailand. Concentrations of heavy metals extracted from microplastics were also reported. The abundance of microplastics in surface water and sediment was 48 ± 8 items/m3 and 39 ± 14 items/kg, respectively. Microplastics with small sizes of 0.05-0.3 mm were predominant in all collected samples. Polypropylene and polyethylene particles were the most abundant in the surface water and sediment, respectively. Toxic metals (Cr, Cu, Ni, Pb, Cd, and Zn) were found in microplastics from surface water samples. Since Chao Phraya River Estuary is an important aquaculture zone in Thailand, the presence of microplastics in this area may severely impact on aquaculture and human health.

Microplastic contamination on the lower Chao Phraya: Abundance, characteristic and interaction with heavy metals.

While a large number of studies on microplastics (MPs) in the western hemisphere have been performed, few studies are available in the eastern hemisphere, especially in Southeast Asia. In this study, the abundance of MPs in water and sediment samples from the Chao Phraya River at the Tha Pra Chan area, Thailand, was investigated. Water samples were collected by a manta trawl, and sediment samples were collected by a Van Veen grab sampler. The average number and concentration of MPs in water samples were 80 ± 65 items/m³ and 53.3 ± 58.4 mg/m³, respectively. The average number and concentration of MPs in sediment samples were 91 ± 13 items/kg and 4.9 ± 3.4 mg/kg, respectively. The main morphologies of MPs were fragments and fibers, mostly white. Polypropylene (PP) was the dominant polymer type found in both water and sediment. Moreover, the content of heavy metals (Cr, Cu, Ni, and Pb) on MPs was extracted by aqua regia solution and analyzed by ICP-OES. Results show high concentrations of Pb and Cu adsorbed on MPs in the study area. In brief, this study suggests that there are high levels of MPs in the water and sediments of the Chao Phraya River at the Tha Pra Chan area.