Effects of intercropping on Se accumulation and growth of pakchoi, lettuce and radish.

Selenium is one of the most basic trace elements in the human body. It is necessary to improve the selenium content in agricultural products through agricultural planting technology to ensure that human nutrition and health need selenium. Therefore, our research passed the effect of intercropping on the growth and selenium accumulation of pakchoi, lettuce and radish were studied through pot experiments to determine whether intercropping of the three crop species can improve their selenium accumulation ability. The results showed that intercropping increased the root and shoot biomass of pakchoi and radish compared with the monocultures, while the biomass of roots and shoots decreased in other intercropping combinations. Intercropping also affected the photosynthetic pigment content of the three crop species. Specifically, the photosynthetic pigments increased in pakchoi and decreased in radish after intercropping. Notably, intercropping the three crop species together increased the SOD (superoxide dismutase) activities of the three crops compared with the monocultures. Meanwhile, intercropping radish with lettuce significantly increased the activities of SOD and CAT (catalase) in radish. Intercropping also increased the soluble sugar content in pakchoi and soluble protein content of radish relative to the monocultures. Furthermore, intercropping decreased the selenium content and the bioconcentration factor of the roots of the three vegetable crops, but improved the shoot selenium content, the bioconcentration factor and the transport factor of Se in pakchoi and radish. In conclusion, intercropping combination of pakchoi and radish can improve selenium accumulation in the edible parts of the crops, which is significant for efficient production of selenium-enriched vegetables.

This research is significant because this study provides some basis for improving the selenium content of plants and efficient production of pakchoi and radish. Under the condition of selenium application in soil, the intercropping of pakchoi and lettuce can promote the growth of both and improve their selenium enrichment ability.

Intercropping affects the physiology and cadmium absorption of pakchoi, lettuce, and radish seedlings.

Intercropping can affect the growth and elemental absorption of vegetables. This study investigated the physiology and cadmium (Cd) content of pakchoi (Brassica chinensis L.), lettuce (Lactuca sativa L. var. ramosa Hort.), and radish (Raphanus sativus L.) seedlings under monoculture, mutual intercropping of two or three varieties. Intercropping is not conducive to the accumulation of chlorophyll and biomass content of pakchoi, lettuce, and radish. When three seedlings were intercropped together, the antioxidant enzyme activity of pakchoi, lettuce, and radish increased and the content of malondialdehyde decreased, except that the superoxide dismutase activity of radish is inferior to the value of radish and pakchoi intercropping. Intercropping increased the soluble sugar and proline content in the lettuce seedlings, while those in the radish and lettuce seedlings reduced or had no significant effect. When intercropped with pakchoi and lettuce, the Cd content in the roots and shoots of pakchoi is higher and lower, respectively. At the same time, root or shoot bio-concentration factors also performed the same trend, and TF was the smallest and less than 1; however, the TF of lettuce is greater than 1. When intercropping with pakchoi or lettuce separately or together, it promoted the accumulation of Cd in radish root; when intercropping with pakchoi, the value of TF was the smallest. From the antioxidant system, the performance of the three seedlings intercropped together is better than the two; however, the accumulation of Cd shows the opposite trend, and the participation of cabbage in the intercropping is relatively conducive to reducing the Cd content in the edible parts.

Potential regulatory genes of light induced anthocyanin accumulation in sweet cherry identified by combining transcriptome and metabolome analysis.

Anthocyanins exist widely in various plant tissues and organs, and they play an important role in plant reproduction, disease resistance, stress resistance, and protection of human vision. Most fruit anthocyanins can be induced to accumulate by light. Here, we shaded the "Hong Deng" sweet cherry and performed an integrated analysis of its transcriptome and metabolome to explore the role of light in anthocyanin accumulation. The total anthocyanin content of the fruit and two of its anthocyanin components were significantly reduced after the shading. Transcriptome and metabolomics analysis revealed that PAL, 4CL, HCT, ANS and other structural genes of the anthocyanin pathway and cyanidin 3-O-glucoside, cyanidin 3-O-rutinoside, and other metabolites were significantly affected by shading. Weighted total gene network analysis and correlation analysis showed that the upstream and middle structural genes 4CL2, 4CL3, and HCT2 of anthocyanin biosynthesis may be the key genes affecting the anthocyanin content variations in fruits after light shading. Their expression levels may be regulated by transcription factors such as LBD, ERF4, NAC2, NAC3, FKF1, LHY, RVE1, and RVE2. This study revealed for the first time the possible role of LBD, FKF1, and other transcription factors in the light-induced anthocyanin accumulation of sweet cherry, thereby laying a preliminary foundation for further research on the role of light in anthocyanin accumulation of deep red fruit varieties and the genetic breeding of sweet cherry.