RESUMEN
Sheep flocks from Hokkaido, Iwate and Aomori, three northern prefectures of Japan, were screened for antibodies to Mycoplasma ovipneumoniae and Mycoplasma agalactiae by ELISA. Sixty four animals out of 246 (26%) were seropositive to M. ovipneumoniae, with positive results obtained from all three prefectures. None of the sera tested were serologically positive to M. agalactiae.
Asunto(s)
Anticuerpos Antibacterianos/sangre , Infecciones por Mycoplasma/veterinaria , Mycoplasma agalactiae/fisiología , Mycoplasma ovipneumoniae/fisiología , Neumonía por Mycoplasma/veterinaria , Enfermedades de las Ovejas/epidemiología , Factores de Edad , Animales , Ensayo de Inmunoadsorción Enzimática/veterinaria , Femenino , Japón/epidemiología , Masculino , Infecciones por Mycoplasma/sangre , Infecciones por Mycoplasma/epidemiología , Infecciones por Mycoplasma/microbiología , Neumonía por Mycoplasma/sangre , Neumonía por Mycoplasma/epidemiología , Neumonía por Mycoplasma/microbiología , Prevalencia , Estudios Seroepidemiológicos , Ovinos , Enfermedades de las Ovejas/sangre , Enfermedades de las Ovejas/microbiologíaRESUMEN
The first epidemiological survey of Border disease virus (BDV) was undertaken in small ruminants in Japan. Ovine sera, collected from the northern prefectures of Hokkaido, Aomori and Iwate, were examined for the presence of antibodies against BDV using the neutralization peroxidase-linked antibody test. Twenty-nine (17.6%) of one hundred and sixty-five samples were seropositive for BDV. Results were specific, excluding cross-reactions with bovine viral diarrhea virus (BVDV). Only one sample (0.6%) was positive for BVDV, and was negative for BDV. Despite serological evidence of virus circulation, there have been no clinical cases of border disease in sheep in Japan. Although no diagnostic measures were performed, the infection did not appear to be associated with a reduction in ewe fertility nor with lamb mortality.
Asunto(s)
Enfermedad de la Frontera/epidemiología , Virus de la Enfermedad de la Frontera/aislamiento & purificación , Animales , Femenino , Japón/epidemiología , Masculino , Estudios Seroepidemiológicos , OvinosRESUMEN
Ovine sera collected from the northern Prefectures of Hokkaido, Iwate and Aomori in Japan, were examined for the presence of antibodies against visna-maedi virus using the agar gel immunodiffusion test and enzyme-linked immunosorbent assays. Three animals (1.12%), out of 267 samples tested, were found to be seropositive to the visna-maedi antigens in both tests. Levels of infection were found in flocks from Hokkaido and Iwate Prefectures, but not in the Aomori Prefecture. Nucleic acid detection by polymerase chain reaction on serum samples did not give positive results. Although no diagnostic measures were in place, the infection could not be related to losses in sheep production or to reduced survival rates. The very limited visna-maedi distribution indicates a highly favourable condition for the application of eradication strategies in this area.