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1.
Proc Natl Acad Sci U S A ; 121(5): e2319475121, 2024 Jan 30.
Artículo en Inglés | MEDLINE | ID: mdl-38252824

RESUMEN

miR-137 is a highly conserved brain-enriched microRNA (miRNA) that has been associated with neuronal function and proliferation. Here, we show that Drosophila miR-137 null mutants display increased body weight with enhanced triglyceride content and decreased locomotor activity. In addition, when challenged by nutrient deprivation, miR-137 mutants exhibit reduced motivation to feed and prolonged survival. We show through genetic epistasis and rescue experiments that this starvation resistance is due to a disruption in insulin signaling. Our studies further show that miR-137 null mutants exhibit a drastic reduction in levels of the phosphorylated/activated insulin receptor, InR (InR-P). We investigated if this is due to the predicted miR-137 target, Protein Tyrosine Phosphatase 61F (PTP61F), ortholog of mammalian TC-PTP/PTP1B, which are known to dephosphorylate InR-P. Indeed, levels of an endogenously tagged GFP-PTP61F are significantly elevated in miR-137 null mutants, and we show that overexpression of PTP61F alone is sufficient to mimic many of the metabolic phenotypes of miR-137 mutants. Finally, we knocked-down elevated levels of PTP61F in the miR-137 null mutant background and show that this rescues levels of InR-P, restores normal body weight and triglyceride content, starvation sensitivity, as well as attenuates locomotor and starvation-induced feeding defects. Our study supports a model in which miR-137 is critical for dampening levels of PTP61F, thereby maintaining normal insulin signaling and energy homeostasis.


Asunto(s)
Proteínas de Drosophila , Insulina , MicroARNs , Proteínas Tirosina Fosfatasas no Receptoras , Transducción de Señal , Animales , Drosophila , Homeostasis , Insulina/metabolismo , Mamíferos , MicroARNs/metabolismo , Monoéster Fosfórico Hidrolasas , Triglicéridos/metabolismo , Proteínas Tirosina Fosfatasas no Receptoras/metabolismo , Proteínas de Drosophila/metabolismo
2.
J Neurosci ; 41(43): 9047-9063, 2021 10 27.
Artículo en Inglés | MEDLINE | ID: mdl-34544836

RESUMEN

Na+ sensitivity is a unique feature of Na+-activated K+ (KNa) channels, making them naturally suited to counter a sudden influx in Na+ ions. As such, it has long been suggested that KNa channels may serve a protective function against excessive excitation associated with neuronal injury and disease. This hypothesis, however, has remained largely untested. Here, we examine KNa channels encoded by the Drosophila Slo2 (dSlo2) gene in males and females. We show that dSlo2/KNa channels are selectively expressed in cholinergic neurons in the adult brain, as well as in glutamatergic motor neurons, where dampening excitation may function to inhibit global hyperactivity and seizure-like behavior. Indeed, we show that effects of feeding Drosophila a cholinergic agonist are exacerbated by the loss of dSlo2/KNa channels. Similar to mammalian Slo2/KNa channels, we show that dSlo2/KNa channels encode a TTX-sensitive K+ conductance, indicating that dSlo2/KNa channels can be activated by Na+ carried by voltage-dependent Na+ channels. We then tested the role of dSlo2/KNa channels in established genetic seizure models in which the voltage-dependent persistent Na+ current (INap) is elevated. We show that the absence of dSlo2/KNa channels increased susceptibility to mechanically induced seizure-like behavior. Similar results were observed in WT flies treated with veratridine, an enhancer of INap Finally, we show that loss of dSlo2/KNa channels in both genetic and pharmacologically primed seizure models resulted in the appearance of spontaneous seizures. Together, our results support a model in which dSlo2/KNa channels, activated by neuronal overexcitation, contribute to a protective threshold to suppress the induction of seizure-like activity.SIGNIFICANCE STATEMENT Slo2/KNa channels are unique in that they constitute a repolarizing K+ pore that is activated by the depolarizing Na+ ion, making them naturally suited to function as a protective "brake" against overexcitation and Na+ overload. Here, we test this hypothesis in vivo by examining how a null mutation of the Drosophila Slo2 (dSlo2)/KNa gene affects seizure-like behavior in genetic and pharmacological models of epilepsy. We show that indeed the loss of dSlo2/KNa channels results in increased incidence and severity of induced seizure behavior, as well as the appearance of spontaneous seizure activity. Our results advance our understanding of neuronal excitability and protective mechanisms that preserve normal physiology and the suppression of seizure susceptibility.


Asunto(s)
Proteínas del Tejido Nervioso/biosíntesis , Canales de potasio activados por Sodio/biosíntesis , Convulsiones/metabolismo , Convulsiones/prevención & control , Animales , Animales Modificados Genéticamente , Drosophila , Femenino , Masculino , Proteínas del Tejido Nervioso/genética , Canales de potasio activados por Sodio/genética , Convulsiones/genética
3.
Exp Cell Res ; 372(1): 1-15, 2018 11 01.
Artículo en Inglés | MEDLINE | ID: mdl-30144444

RESUMEN

Regulating the residence time of membrane proteins on the cell surface can modify their response to extracellular cues and allow for cellular adaptation in response to changing environmental conditions. The fate of membrane proteins that are internalized from the plasma membrane and arrive at the limiting membrane of the late endosome/multivesicular body (MVB) is dictated by whether they remain on the limiting membrane, bud into internal MVB vesicles, or bud outwardly from the membrane. The molecular details underlying the disposition of membrane proteins that transit this pathway and the mechanisms regulating these trafficking events are unclear. We established a cell-free system that reconstitutes budding of membrane protein cargo into internal MVB vesicles and onto vesicles that bud outwardly from the MVB membrane. Both budding reactions are cytosol-dependent and supported by Saccharomyces cerevisiae (yeast) cytosol. We observed that inward and outward budding from the MVB membrane are mechanistically distinct but may be linked, such that inhibition of inward budding triggers a re-routing of cargo from inward to outward budding vesicles, without affecting the number of vesicles that bud outwardly from MVBs.


Asunto(s)
Membrana Celular/metabolismo , Complejos de Clasificación Endosomal Requeridos para el Transporte/metabolismo , Membranas Intracelulares/metabolismo , Lisosomas/metabolismo , Cuerpos Multivesiculares/metabolismo , Proteínas Adaptadoras Transductoras de Señales/genética , Proteínas Adaptadoras Transductoras de Señales/metabolismo , Membrana Celular/química , Sistema Libre de Células/química , Sistema Libre de Células/metabolismo , Complejos de Clasificación Endosomal Requeridos para el Transporte/genética , Complejos de Clasificación Endosomal Requeridos para el Transporte/ultraestructura , Regulación de la Expresión Génica , Células HeLa , Humanos , Membranas Intracelulares/ultraestructura , Lisosomas/ultraestructura , Cuerpos Multivesiculares/ultraestructura , Fosfoproteínas/genética , Fosfoproteínas/metabolismo , Transporte de Proteínas , Saccharomyces cerevisiae/química , Saccharomyces cerevisiae/metabolismo , Transducción de Señal
4.
PLoS Genet ; 11(3): e1005025, 2015 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-25774758

RESUMEN

Alzheimer's disease (AD) is the most prevalent form of dementia in the elderly. ß-amyloid (Aß) accumulation in the brain is thought to be a primary event leading to eventual cognitive and motor dysfunction in AD. Aß has been shown to promote neuronal hyperactivity, which is consistent with enhanced seizure activity in mouse models and AD patients. Little, however, is known about whether, and how, increased excitability contributes to downstream pathologies of AD. Here, we show that overexpression of human Aß42 in a Drosophila model indeed induces increased neuronal activity. We found that the underlying mechanism involves the selective degradation of the A-type K+ channel, Kv4. An age-dependent loss of Kv4 leads to an increased probability of AP firing. Interestingly, we find that loss of Kv4 alone results in learning and locomotion defects, as well as a shortened lifespan. To test whether the Aß42-induced increase in neuronal excitability contributes to, or exacerbates, downstream pathologies, we transgenically over-expressed Kv4 to near wild-type levels in Aß42-expressing animals. We show that restoration of Kv4 attenuated age-dependent learning and locomotor deficits, slowed the onset of neurodegeneration, and partially rescued premature death seen in Aß42-expressing animals. We conclude that Aß42-induced hyperactivity plays a critical role in the age-dependent cognitive and motor decline of this Aß42-Drosophila model, and possibly in AD.


Asunto(s)
Enfermedad de Alzheimer/metabolismo , Enfermedad de Alzheimer/patología , Péptidos beta-Amiloides/metabolismo , Fragmentos de Péptidos/metabolismo , Animales , Animales Modificados Genéticamente , Modelos Animales de Enfermedad , Drosophila , Humanos , Lisosomas/metabolismo , Ratones , Neuronas/metabolismo , Complejo de la Endopetidasa Proteasomal/metabolismo , Canales de Potasio Shal/metabolismo
5.
Neuron ; 57(1): 69-79, 2008 Jan 10.
Artículo en Inglés | MEDLINE | ID: mdl-18184565

RESUMEN

Neutral ceramidase, a key enzyme of sphingolipid metabolism, hydrolyzes ceramide to sphingosine. These sphingolipids are critical structural components of cell membranes and act as second messengers in diverse signal transduction cascades. Here, we have isolated and characterized functional null mutants of Drosophila ceramidase. We show that secreted ceramidase functions in a cell-nonautonomous manner to maintain photoreceptor homeostasis. In the absence of ceramidase, photoreceptors degenerate in a light-dependent manner, are defective in normal endocytic turnover of rhodopsin, and do not respond to light stimulus. Consistent with a cell-nonautonomous function, overexpression of ceramidase in tissues distant from photoreceptors suppresses photoreceptor degeneration in an arrestin mutant and facilitates membrane turnover in a rhodopsin null mutant. Furthermore, our results show that secreted ceramidase is internalized and localizes to endosomes. Our findings establish a role for a secreted sphingolipid enzyme in the regulation of photoreceptor structure and function.


Asunto(s)
Amidohidrolasas/fisiología , Proteínas de Drosophila/metabolismo , Regulación del Desarrollo de la Expresión Génica/fisiología , Homeostasis/fisiología , Células Fotorreceptoras de Invertebrados/fisiología , Animales , Animales Modificados Genéticamente , Apoptosis/genética , Apoptosis/efectos de la radiación , Arrestina/metabolismo , Ceramidasas , Drosophila , Proteínas de Drosophila/genética , Electrorretinografía/métodos , Embrión no Mamífero , Ojo/metabolismo , Ojo/ultraestructura , Cuerpo Adiposo/metabolismo , Cuerpo Adiposo/ultraestructura , Potenciales de la Membrana/genética , Potenciales de la Membrana/efectos de la radiación , Mutación/fisiología , Estimulación Luminosa/métodos , Unión Proteica/genética , Degeneración Retiniana/etiología , Degeneración Retiniana/genética , Degeneración Retiniana/metabolismo , Rodopsina/metabolismo , Esfingosina/metabolismo
6.
Mol Cell Neurosci ; 45(1): 75-83, 2010 Sep.
Artículo en Inglés | MEDLINE | ID: mdl-20550966

RESUMEN

Shal K(+) (K(v)4) channels in mammalian neurons have been shown to be localized exclusively to somato-dendritic regions of neurons, where they function as key determinants of dendritic excitability. To gain insight into the mechanisms underlying dendritic localization of K(v)4 channels, we use Drosophila melanogaster as our model system. We show that Shal K(+) channels display a conserved somato-dendritic localization in vivo in Drosophila. From a yeast-2-hybrid screen, we identify the novel interactor, SIDL (for Shal Interactor of Di-Leucine Motif), as the first target protein reported to bind the highly conserved di-leucine motif (LL-motif) implicated in dendritic targeting. We show that SIDL is expressed primarily in the nervous system, co-localizes with GFP-Shal channels in neurons, and interacts specifically with the LL-motif of Drosophila and mouse Shal channels. We disrupt the Shal-SIDL interaction by mutating the LL-motif on Shal channels, and show that Shal K(+) channels are then mislocalized to some, but not all, axons in vivo. These results suggest that there are multiple mechanisms underlying Shal K(+) channel targeting, one of which depends on the LL-motif. The identification of SIDL may provide the first step for future investigation into the molecular machinery regulating the LL-motif-dependent targeting of K(+) channels.


Asunto(s)
Dendritas/metabolismo , Proteínas de Drosophila/metabolismo , Drosophila melanogaster/metabolismo , Canales de Potasio Shal/metabolismo , Animales , Proteínas de Drosophila/genética , Drosophila melanogaster/anatomía & histología , Drosophila melanogaster/embriología , Humanos , Ratones , Neuronas/citología , Neuronas/metabolismo , Proteínas Recombinantes de Fusión/genética , Proteínas Recombinantes de Fusión/metabolismo , Canales de Potasio Shal/genética , Técnicas del Sistema de Dos Híbridos
7.
PLoS One ; 16(12): e0261087, 2021.
Artículo en Inglés | MEDLINE | ID: mdl-34932577

RESUMEN

Age-related changes in ion channel expression are likely to affect neuronal signaling. Here, we examine how age affects Kv4/Shal and Kv1/Shaker K+ channel protein levels in Drosophila. We show that Kv4/Shal protein levels decline sharply from 3 days to 10 days, then more gradually from 10 to 40 days after eclosion. In contrast, Kv1/Shaker protein exhibits a transient increase at 10 days that then stabilizes and eventually declines at 40 days. We present data that begin to show a relationship between reactive oxygen species (ROS), Kv4/Shal, and locomotor performance. We show that Kv4/Shal levels are negatively affected by ROS, and that over-expression of Catalase or RNAi knock-down of the ROS-generating enzyme, Nicotinamide Adenine Dinucleotide Phosphate (NADPH) Oxidase (NOX), can attenuate the loss of Kv4/Shal protein. Finally, we compare levels of Kv4.2 and Kv4.3 in the hippocampus, olfactory bulb, cerebellum, and motor cortex of mice aged 6 weeks and 1 year. While there was no global decline in Kv4.2/4.3 that parallels what we report in Drosophila, we did find that Kv4.2/4.3 are differentially affected in various brain regions; this survey of changes may help inform mammalian studies that examine neuronal function with age.


Asunto(s)
Potenciales de Acción , Proteínas de Drosophila/metabolismo , Drosophila melanogaster/metabolismo , Neuronas/fisiología , Especies Reactivas de Oxígeno/metabolismo , Canales de Potasio de la Superfamilia Shaker/metabolismo , Canales de Potasio Shal/metabolismo , Factores de Edad , Animales , Proteínas de Drosophila/genética , Drosophila melanogaster/genética , Masculino , Neuronas/citología , Canales de Potasio de la Superfamilia Shaker/genética , Canales de Potasio Shal/genética
8.
Mol Cell Neurosci ; 42(1): 33-44, 2009 Sep.
Artículo en Inglés | MEDLINE | ID: mdl-19463952

RESUMEN

Shal K+ (K(v)4) channels across species carry the major A-type K+ current present in neurons. Shal currents are activated by small EPSPs and modulate post-synaptic potentials, backpropagation of action potentials, and induction of LTP. Fast inactivation of Shal channels regulates the impact of this post-synaptic modulation. Here, we introduce SKIP3, as the first protein interactor of Drosophila Shal K+ channels. The SKIP gene encodes three isoforms with multiple protein-protein interaction domains. SKIP3 is nervous system specific and co-localizes with Shal channels in neuronal cell bodies, and in puncta along processes. Using a genetic deficiency of SKIP, we show that the proportion of neurons displaying a very fast inactivation, consistent with Shal channels exclusively in a "fast" gating mode, is increased in the absence of SKIP3. As a scaffold-like protein, SKIP3 is likely to lead to the identification of a novel regulatory complex that modulates Shal channel inactivation.


Asunto(s)
Proteínas de Drosophila/metabolismo , Regulación de la Expresión Génica/fisiología , Neuronas/fisiología , Proteínas Serina-Treonina Quinasas/metabolismo , Canales de Potasio Shal/fisiología , Animales , Animales Modificados Genéticamente , Biofisica , Línea Celular , Células Cultivadas , Drosophila , Proteínas de Drosophila/deficiencia , Proteínas de Drosophila/genética , Estimulación Eléctrica/métodos , Embrión no Mamífero , Regulación de la Expresión Génica/genética , Proteínas Fluorescentes Verdes/genética , Potenciales de la Membrana/genética , Potenciales de la Membrana/fisiología , Técnicas de Placa-Clamp/métodos , Proteínas Serina-Treonina Quinasas/deficiencia , Proteínas Serina-Treonina Quinasas/genética , Transfección/métodos
9.
Cell Rep ; 32(10): 108119, 2020 09 08.
Artículo en Inglés | MEDLINE | ID: mdl-32905767

RESUMEN

Homeostatic synaptic plasticity (HSP) involves compensatory mechanisms employed by neurons and circuits to preserve signaling when confronted with global changes in activity that may occur during physiological and pathological conditions. Cholinergic neurons, which are especially affected in some pathologies, have recently been shown to exhibit HSP mediated by nicotinic acetylcholine receptors (nAChRs). In Drosophila central neurons, pharmacological blockade of activity induces a homeostatic response mediated by the Drosophila α7 (Dα7) nAChR, which is tuned by a subsequent increase in expression of the voltage-dependent Kv4/Shal channel. Here, we show that an in vivo reduction of cholinergic signaling induces HSP mediated by Dα7 nAChRs, and this upregulation of Dα7 itself is sufficient to trigger transcriptional activation, mediated by nuclear factor of activated T cells (NFAT), of the Kv4/Shal gene, revealing a receptor-ion channel system coupled for homeostatic tuning in cholinergic neurons.


Asunto(s)
Proteínas de Drosophila/metabolismo , Canales de Potasio Shal/metabolismo , Transmisión Sináptica/genética , Receptor Nicotínico de Acetilcolina alfa 7/metabolismo , Animales , Homeostasis
10.
J Neurosci ; 28(1): 304-14, 2008 Jan 02.
Artículo en Inglés | MEDLINE | ID: mdl-18171947

RESUMEN

The synaptic membrane-associated guanylate kinase (MAGUK) scaffolding protein family is thought to play key roles in synapse assembly and synaptic plasticity. Evidence supporting these roles in vivo is scarce, as a consequence of gene redundancy in mammals. The genome of Drosophila contains only one MAGUK gene, discs large (dlg), from which two major proteins originate: DLGA [PSD95 (postsynaptic density 95)-like] and DLGS97 [SAP97 (synapse-associated protein)-like]. These differ only by the inclusion in DLGS97 of an L27 domain, important for the formation of supramolecular assemblies. Known dlg mutations affect both forms and are lethal at larval stages attributable to tumoral overgrowth of epithelia. We generated independent null mutations for each, dlgA and dlgS97. These allowed unveiling of a shift in expression during the development of the nervous system: predominant expression of DLGA in the embryo, balanced expression of both during larval stages, and almost exclusive DLGS97 expression in the adult brain. Loss of embryonic DLGS97 does not alter the development of the nervous system. At larval stages, DLGA and DLGS97 fulfill both unique and partially redundant functions in the neuromuscular junction. Contrary to dlg and dlgA mutants, dlgS97 mutants are viable to adulthood, but they exhibit marked alterations in complex behaviors such as phototaxis, circadian activity, and courtship, whereas simpler behaviors like locomotion and odor and light perception are spared. We propose that the increased repertoire of associations of a synaptic scaffold protein given by an additional domain of protein-protein interaction underlies its ability to integrate molecular networks required for complex functions in adult synapses.


Asunto(s)
Conducta Animal/fisiología , Proteínas de Drosophila/metabolismo , Regulación del Desarrollo de la Expresión Génica/fisiología , Unión Neuromuscular/fisiología , Isoformas de Proteínas/metabolismo , Proteínas Supresoras de Tumor/metabolismo , Animales , Animales Modificados Genéticamente , Ritmo Circadiano/fisiología , Drosophila , Proteínas de Drosophila/genética , Embrión no Mamífero , Proteínas Fluorescentes Verdes/genética , Proteínas Fluorescentes Verdes/metabolismo , Potenciales de la Membrana/fisiología , Microscopía Electrónica de Transmisión/métodos , Actividad Motora , Mutación/fisiología , Unión Neuromuscular/ultraestructura , Isoformas de Proteínas/genética , Conducta Sexual Animal/fisiología , Proteínas Supresoras de Tumor/genética
11.
Neurobiol Aging ; 84: 166-177, 2019 12.
Artículo en Inglés | MEDLINE | ID: mdl-31629115

RESUMEN

Beta-amyloid (Aß) peptide accumulation has long been implicated in the pathogenesis of Alzheimer's disease (AD). Hippocampal network hyperexcitability in the early stages of the disease leads to increased epileptiform activity and eventually cognitive decline. We found that acute application of 250 nM soluble Aß42 oligomers increased Ca2+ activity in hippocampal neurons in parallel with a significant decrease in activity in Aß42-treated interneurons. A potential target of Aß42 is the nicotinic acetylcholine receptor (nAChR). Three major subtypes of nAChRs (α7, α4ß2, and α3ß4) have been reported in the human hippocampus. Simultaneous inhibition of both α7 and α4ß2 nAChRs mimicked the Aß42 effects on both excitatory and inhibitory neurons. However, inhibition of all 3 subtypes showed the opposite effect. Importantly, simultaneous activation of α7 and α4ß2 nAChRs was required to reverse Aß42-induced neuronal hyperexcitation. We suggest co-activation of α7 and α4ß2 nAChRs is required to reverse Aß42-induced Ca2+ hyperexcitation.


Asunto(s)
Péptidos beta-Amiloides/metabolismo , Encéfalo/metabolismo , Calcio/metabolismo , Receptores Nicotínicos/metabolismo , Enfermedad de Alzheimer , Humanos
12.
Cell Rep ; 24(2): 342-354, 2018 07 10.
Artículo en Inglés | MEDLINE | ID: mdl-29996096

RESUMEN

Homeostatic synaptic plasticity (HSP) is the ability of neurons to exert compensatory changes in response to altered neural activity. How pathologically induced activity changes are intertwined with HSP mechanisms is unclear. We show that, in cholinergic neurons from Drosophila, beta-amyloid (Aß) peptides Aß40 and Aß42 both induce an increase in spontaneous activity. In a transgenic line expressing Aß42, we observe that this early increase in spontaneous activity is followed by a dramatic reduction in spontaneous events, a progression that has been suggested to occur in cholinergic brain regions of mammalian models of Alzheimer's disease. We present evidence that the early enhancement in synaptic activity is mediated by the Drosophila α7 nicotinic acetylcholine receptor (nAChR) and that, later, Aß42-induced inhibition of synaptic events is a consequence of Dα7-dependent HSP mechanisms induced by earlier hyperactivity. Thus, while HSP may initially be an adaptive response, it may also drive maladaptive changes and downstream pathologies.


Asunto(s)
Péptidos beta-Amiloides/toxicidad , Colinérgicos/metabolismo , Homeostasis , Plasticidad Neuronal , Neuronas/metabolismo , Animales , Drosophila melanogaster/efectos de los fármacos , Drosophila melanogaster/metabolismo , Potenciales Postsinápticos Excitadores/efectos de los fármacos , Homeostasis/efectos de los fármacos , Humanos , Cinética , Inhibición Neural/efectos de los fármacos , Plasticidad Neuronal/efectos de los fármacos , Neuronas/efectos de los fármacos , Ratas , Sinapsis/efectos de los fármacos , Sinapsis/metabolismo , Receptor Nicotínico de Acetilcolina alfa 7/metabolismo
13.
Methods Mol Biol ; 1270: 115-24, 2015.
Artículo en Inglés | MEDLINE | ID: mdl-25702113

RESUMEN

The signaling activity of cell surface localized membrane proteins occurs primarily while these proteins are located on the plasma membrane but is, in some cases, not terminated until the proteins are degraded. Following internalization and movement through the endocytic pathway en route to lysosomes, membrane proteins transit a late endosomal organelle called the multivesicular body (MVB). MVBs are formed by invagination of the limiting membrane of endosomes, resulting in an organelle possessing a limiting membrane and containing internal vesicles. The fate of an internalized membrane protein depends on whether it buds outwardly from the endosomal membrane, promoting recycling and continued signaling, or is internalized into internal MVB vesicles and is ultimately degraded upon MVB-lysosome fusion. The molecular machinery that regulates the separation of membrane proteins destined for degradation from those resulting in surface expression is not well understood.To elucidate the molecular mechanisms that underlie membrane protein sorting, we have reconstituted an endosomal sorting event under cell-free conditions. We took advantage of the itinerary of a prototypical membrane protein, the epidermal growth factor receptor (EGFR) and designed a biochemical monitor for cargo movement into internal MVB vesicles that is generally modifiable for other membrane proteins. Since is it not known how internal vesicle formation is related to cargo sorting, morphological examination using transmission electron microscopy (TEM) allows separate monitoring of vesicle formation. We have determined that MVB sorting is dependent on cytosolic components, adenosine triphosphate (ATP), time, temperature, and an intact proton gradient. This assay reconstitutes the maturation of late endosomes and allows the morphological and biochemical examination of vesicle formation and membrane protein sorting.


Asunto(s)
Sistema Libre de Células , Cuerpos Multivesiculares/metabolismo , Proteínas/metabolismo , Animales , Membrana Celular/metabolismo , Drosophila melanogaster/metabolismo , Transporte de Proteínas , Saccharomyces cerevisiae/metabolismo , Proteínas de Saccharomyces cerevisiae/metabolismo
14.
Fly (Austin) ; 6(3): 153-7, 2012.
Artículo en Inglés | MEDLINE | ID: mdl-22735167

RESUMEN

Synaptic homeostasis is a form of neuronal plasticity that stabilizes activity of neural networks. Both presynaptic and postsynaptic effects are well documented in response to activity changes. The electrical signaling machinery of individual neurons, or intrinsic properties, have also been implicated in this plasticity. How synaptic and intrinsic changes are coordinated, however, is still a puzzle. A recent study by Ping and Tsunoda shows both synaptic and intrinsic changes in Drosophila central neurons in response to prolonged inactivity. ( 1) Changes include the upregulation of Dα7 nicotinic acetylcholine receptors (nAChRs) and Shal (Kv 4) potassium channels. This work has two noteworthy findings. First, although mediated by different receptors, synaptic homeostasis in the central nervous system (CNS) is conserved across species. This is perhaps the most direct demonstration that nAChRs mediate synaptic homeostasis. Changes in the expression of nAChRs have long been noted during development, as well as during pathological conditions, such as nicotine addiction ( 2) and Alzheimer disease. ( 3) The second interesting finding is the relationship between synaptic and intrinsic plasticity: nAChRs are upregulated immediately, subsequently triggering a rapid increase in Shal K (+) channels. This novel mechanism regulates synaptic homeostasis to stabilize synaptic potentials. This study sets the stage for Drosophila central neurons as a model for cholinergic synaptic homeostasis, its regulation and role in disease.


Asunto(s)
Sistema Nervioso Central/fisiología , Drosophila/citología , Modelos Biológicos , Animales , Sistema Nervioso Central/patología , Homeostasis , Humanos , Potenciales Sinápticos
15.
PLoS One ; 7(2): e31622, 2012.
Artículo en Inglés | MEDLINE | ID: mdl-22363689

RESUMEN

BACKGROUND: TRP channels function as key mediators of sensory transduction and other cellular signaling pathways. In Drosophila, TRP and TRPL are the light-activated channels in photoreceptors. While TRP is statically localized in the signaling compartment of the cell (the rhabdomere), TRPL localization is regulated by light. TRPL channels translocate out of the rhabdomere in two distinct stages, returning to the rhabdomere with dark-incubation. Translocation of TRPL channels regulates their availability, and thereby the gain of the signal. Little, however, is known about the mechanisms underlying this trafficking of TRPL channels. METHODOLOGY/PRINCIPAL FINDINGS: We first examine the involvement of de novo protein synthesis in TRPL translocation. We feed flies cycloheximide, verify inhibition of protein synthesis, and test for TRPL translocation in photoreceptors. We find that protein synthesis is not involved in either stage of TRPL translocation out of the rhabdomere, but that re-localization to the rhabdomere from stage-1, but not stage-2, depends on protein synthesis. We also characterize an ex vivo eye preparation that is amenable to biochemical and genetic manipulation. We use this preparation to examine mechanisms of stage-1 TRPL translocation. We find that stage-1 translocation is: induced with ATP depletion, unaltered with perturbation of the actin cytoskeleton or inhibition of endocytosis, and slowed with increased membrane sterol content. CONCLUSIONS/SIGNIFICANCE: Our results indicate that translocation of TRPL out of the rhabdomere is likely due to protein transport, and not degradation/re-synthesis. Re-localization from each stage to the rhabdomere likely involves different strategies. Since TRPL channels can translocate to stage-1 in the absence of ATP, with no major requirement of the cytoskeleton, we suggest that stage-1 translocation involves simple diffusion through the apical membrane, which may be regulated by release of a light-dependent anchor in the rhabdomere.


Asunto(s)
Proteínas de Drosophila/metabolismo , Drosophila melanogaster/metabolismo , Células Fotorreceptoras de Invertebrados/metabolismo , Canales de Potencial de Receptor Transitorio/metabolismo , Citoesqueleto de Actina/efectos de los fármacos , Citoesqueleto de Actina/metabolismo , Citoesqueleto de Actina/efectos de la radiación , Adenosina Trifosfato/farmacología , Animales , Membrana Celular/efectos de los fármacos , Membrana Celular/metabolismo , Membrana Celular/efectos de la radiación , Citocalasina D/farmacología , Oscuridad , Dieta , Drosophila melanogaster/citología , Drosophila melanogaster/efectos de la radiación , Dinaminas/metabolismo , Endocitosis/efectos de los fármacos , Endocitosis/efectos de la radiación , Ergosterol/metabolismo , Técnicas In Vitro , Cinética , Luz , Células Fotorreceptoras de Invertebrados/citología , Células Fotorreceptoras de Invertebrados/efectos de los fármacos , Células Fotorreceptoras de Invertebrados/efectos de la radiación , Biosíntesis de Proteínas/efectos de los fármacos , Biosíntesis de Proteínas/efectos de la radiación , Transporte de Proteínas/efectos de los fármacos , Transporte de Proteínas/efectos de la radiación
16.
Nat Neurosci ; 15(1): 90-7, 2011 Nov 13.
Artículo en Inglés | MEDLINE | ID: mdl-22081160

RESUMEN

Long-term synaptic changes, which are essential for learning and memory, are dependent on homeostatic mechanisms that stabilize neural activity. Homeostatic responses have also been implicated in pathological conditions, including nicotine addiction. Although multiple homeostatic pathways have been described, little is known about how compensatory responses are tuned to prevent them from overshooting their optimal range of activity. We found that prolonged inhibition of nicotinic acetylcholine receptors (nAChRs), the major excitatory receptors in the Drosophila CNS, resulted in a homeostatic increase in the Drosophila α7 (Dα7)-nAChR. This response then induced an increase in the transient A-type K(+) current carried by Shaker cognate L (Shal; also known as voltage-gated K(+) channel 4, Kv4) channels. Although increasing Dα7-nAChRs boosted miniature excitatory postsynaptic currents, the ensuing increase in Shal channels served to stabilize postsynaptic potentials. These data identify a previously unknown mechanism for fine tuning the homeostatic response.


Asunto(s)
Potenciales Postsinápticos Excitadores/fisiología , Potenciales Postsinápticos Miniatura/fisiología , Receptores Nicotínicos/metabolismo , Canales de Potasio Shal/metabolismo , Sinapsis/metabolismo , Transmisión Sináptica/fisiología , Animales , Drosophila , Homeostasis/fisiología , Neuronas/metabolismo , Receptores Nicotínicos/genética , Canales de Potasio Shal/genética , Sinapsis/genética
17.
PLoS One ; 6(1): e16043, 2011 Jan 17.
Artículo en Inglés | MEDLINE | ID: mdl-21264215

RESUMEN

BACKGROUND: Rhythmic behaviors, such as walking and breathing, involve the coordinated activity of central pattern generators in the CNS, sensory feedback from the PNS, to motoneuron output to muscles. Unraveling the intrinsic electrical properties of these cellular components is essential to understanding this coordinated activity. Here, we examine the significance of the transient A-type K(+) current (I(A)), encoded by the highly conserved Shal/K(v)4 gene, in neuronal firing patterns and repetitive behaviors. While I(A) is present in nearly all neurons across species, elimination of I(A) has been complicated in mammals because of multiple genes underlying I(A), and/or electrical remodeling that occurs in response to affecting one gene. METHODOLOGY/PRINCIPAL FINDINGS: In Drosophila, the single Shal/K(v)4 gene encodes the predominant I(A) current in many neuronal cell bodies. Using a transgenically expressed dominant-negative subunit (DNK(v)4), we show that I(A) is completely eliminated from cell bodies, with no effect on other currents. Most notably, DNK(v)4 neurons display multiple defects during prolonged stimuli. DNK(v)4 neurons display shortened latency to firing, a lower threshold for repetitive firing, and a progressive decrement in AP amplitude to an adapted state. We record from identified motoneurons and show that Shal/K(v)4 channels are similarly required for maintaining excitability during repetitive firing. We then examine larval crawling, and adult climbing and grooming, all behaviors that rely on repetitive firing. We show that all are defective in the absence of Shal/K(v)4 function. Further, knock-out of Shal/K(v)4 function specifically in motoneurons significantly affects the locomotion behaviors tested. CONCLUSIONS/SIGNIFICANCE: Based on our results, Shal/K(v)4 channels regulate the initiation of firing, enable neurons to continuously fire throughout a prolonged stimulus, and also influence firing frequency. This study shows that Shal/K(v)4 channels play a key role in repetitively firing neurons during prolonged input/output, and suggests that their function and regulation are important for rhythmic behaviors.


Asunto(s)
Potenciales de Acción/fisiología , Proteínas de Drosophila/fisiología , Drosophila melanogaster/fisiología , Locomoción/fisiología , Canales de Potasio Shal/fisiología , Animales , Animales Modificados Genéticamente , Neuronas/fisiología , Periodicidad , Transmisión Sináptica
18.
Fly (Austin) ; 4(2): 95-103, 2010.
Artículo en Inglés | MEDLINE | ID: mdl-20404479

RESUMEN

In Drosophila photoreceptors, the multivalent PDZ protein INAD interacts with multiple signaling components and localizes complexes to the rhabdomere, a subcellular compartment specialized for phototransduction. Since this localization is critical for signaling, we conducted a genetic screen of the third chromosome for mutations that result in mislocalization of an INAD-GFP fusion protein. We identified seven mutant lines that fall into two complementation groups, idl (INAD localization)-A and idl-B. We show that idl-A mutants fail to complement with chaoptic (chp) mutants. Since chaoptin is a structural component of the rhabdomere, mislocalization of INAD may be a secondary effect of the retinal degeneration in chp and idl-A mutants. Genetic complementation and DNA sequencing reveal that the two idl-B mutants represent new alleles of trp, a gene encoding the major light-activated channel. The molecular change in each allele affects a highly conserved residue in either an ankyrin domain on the N-terminus or in the S6 transmembrane domain of TRP. These changes lead to the loss of TRP protein. TRP has previously been shown to anchor INAD in the rhabdomeres, therefore the independent identification of two trp alleles validates our screen for INAD-GFP localization. One possibility is that a limited number of proteins are required for localizing INAD-signaling complexes. A similar screen of the X and second chromosomes may be required to find the remaining players involved.


Asunto(s)
Proteínas de Drosophila/genética , Proteínas de Drosophila/metabolismo , Drosophila melanogaster/genética , Drosophila melanogaster/metabolismo , Proteínas del Ojo/genética , Proteínas del Ojo/metabolismo , Células Fotorreceptoras de Invertebrados/metabolismo , Alelos , Secuencia de Aminoácidos , Animales , Animales Modificados Genéticamente , Drosophila melanogaster/citología , Femenino , Genes de Insecto , Prueba de Complementación Genética , Pruebas Genéticas , Proteínas Fluorescentes Verdes/genética , Proteínas Fluorescentes Verdes/metabolismo , Masculino , Glicoproteínas de Membrana/genética , Glicoproteínas de Membrana/metabolismo , Modelos Moleculares , Datos de Secuencia Molecular , Complejos Multiproteicos , Proteínas Mutantes/genética , Proteínas Mutantes/metabolismo , Mutación , Proteínas Recombinantes de Fusión/genética , Proteínas Recombinantes de Fusión/metabolismo , Degeneración Retiniana/genética , Degeneración Retiniana/metabolismo , Homología de Secuencia de Aminoácido , Canales de Potencial de Receptor Transitorio/genética , Canales de Potencial de Receptor Transitorio/metabolismo
19.
Mol Cell Neurosci ; 36(1): 36-46, 2007 Sep.
Artículo en Inglés | MEDLINE | ID: mdl-17689976

RESUMEN

Here, we reveal a novel feature of the dynamic organization of signaling components in Drosophila photoreceptors. We show that the multi-PDZ protein INAD and its target proteins undergo light-induced recruitment to detergent-resistant membrane (DRM) rafts. Reduction of ergosterol, considered to be a key component of lipid rafts in Drosophila, resulted in a loss of INAD-signaling complexes associated with DRM fractions. Genetic analysis demonstrated that translocation of INAD-signaling complexes to DRM rafts requires activation of the entire phototransduction cascade, while constitutive activation of the light-activated channels resulted in recruitment of complexes to DRM rafts in the dark. Mutations affecting INAD and TRP showed that PDZ4 and PDZ5 domains of INAD, as well as the INAD-TRP interaction, are required for translocation of components to DRM rafts. Finally, selective recruitment of phosphorylated, and therefore activatable, eye-PKC to DRM rafts suggests that DRM domains are likely to function in signaling, rather than trafficking.


Asunto(s)
Detergentes/farmacología , Proteínas de Drosophila/metabolismo , Proteínas del Ojo/metabolismo , Microdominios de Membrana , Células Fotorreceptoras de Invertebrados/citología , Transducción de Señal , Animales , Animales Modificados Genéticamente , Drosophila , Regulación de la Expresión Génica/fisiología , Luz , Microdominios de Membrana/efectos de los fármacos , Microdominios de Membrana/metabolismo , Microdominios de Membrana/efectos de la radiación , Neuronas Motoras/efectos de los fármacos , Neuronas Motoras/metabolismo , Células Fotorreceptoras de Invertebrados/efectos de la radiación , Transducción de Señal/efectos de los fármacos , Transducción de Señal/fisiología , Transducción de Señal/efectos de la radiación
20.
J Cell Sci ; 119(Pt 14): 2935-44, 2006 Jul 15.
Artículo en Inglés | MEDLINE | ID: mdl-16787936

RESUMEN

Transient receptor potential (TRP) channels across species are expressed in sensory receptor cells, and often localized to specialized subcellular sites. In Drosophila photoreceptors, TRP-like (TRPL) channels are localized to the signaling compartment, the rhabdomere, in the dark, and undergo light-induced translocation into the cell body as a mechanism for long-term light-adaptation. We show that translocation of TRPL channels occurs in two distinct stages, first to the neighboring stalk membrane then to the basolateral membrane. In the first stage, light-induced translocation occurs within 5 minutes, whereas the second stage takes over 6 hours. The exclusive apical localization of TRPL channels in the first stage of translocation suggests that channels are released from the rhabdomere and diffuse laterally through the membrane into the adjoining stalk membrane. In the second stage, TRPL channels are localized in the basolateral membrane, implicating a different transport mechanism. Genetic analyses suggest that activation of the other light-activated TRP channel and eye-protein-kinase C (eye-PKC) are both required for the second stage of TRPL translocation in R1 to R6 photoreceptor cells, whereas only phospholipase C (PLC) is required for the first stage. Finally, we show that arrestin2 is required for the rhabdomeric localization and stability of TRPL channels.


Asunto(s)
Proteínas de Drosophila/metabolismo , Drosophila melanogaster/metabolismo , Drosophila melanogaster/efectos de la radiación , Luz , Células Fotorreceptoras de Invertebrados/metabolismo , Células Fotorreceptoras de Invertebrados/efectos de la radiación , Canales de Potencial de Receptor Transitorio/metabolismo , Animales , Arrestinas/metabolismo , Oscuridad , Drosophila melanogaster/anatomía & histología , Modelos Biológicos , Muda/efectos de la radiación , Mutación/genética , Células Fotorreceptoras de Invertebrados/citología , Proteína Quinasa C/metabolismo , Transporte de Proteínas/efectos de la radiación , Pupa/metabolismo , Pupa/efectos de la radiación , Transducción de Señal , Termodinámica , Factores de Tiempo
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