RESUMEN
RNA editing alters the identity of nucleotides in RNA molecules such that the information for a protein in the mRNA differs from the prediction of the genomic DNA. In chloroplasts and mitochondria of flowering plants, RNA editing changes C nucleotides to U nucleotides; in ferns and mosses, it also changes U to C. The approximately 500 editing sites in mitochondria and 40 editing sites in plastids of flowering plants are individually addressed by specific proteins, genes for which are amplified in plant species with organellar RNA editing. These proteins contain repeat elements that bind to cognate RNA sequence motifs just 5' to the edited nucleotide. In flowering plants, the site-specific proteins interact selectively with individual members of a different, smaller family of proteins. These latter proteins may be connectors between the site-specific proteins and the as yet unknown deaminating enzymatic activity.
Asunto(s)
Plantas/genética , Edición de ARN , ARN de Planta/genética , Proteínas de Arabidopsis/genética , Codón/genética , Evolución Molecular , Mitocondrias/genética , Proteínas de Plantas/genética , Proteínas de Plantas/fisiología , Plastidios/genética , ARN Mensajero/genéticaRESUMEN
RNA editing in plastids and mitochondria of flowering plants changes hundreds of selected cytidines to uridines, mostly in coding regions of mRNAs. Specific sequences around the editing sites are presumably recognized by up to 200 pentatricopeptide repeat (PPR) proteins. The here identified family of multiple organellar RNA editing factor (MORF) proteins provides additional components of the RNA editing machinery in both plant organelles. Two MORF proteins are required for editing in plastids; at least two are essential for editing in mitochondria. The loss of a MORF protein abolishes or lowers editing at multiple sites, many of which are addressed individually by PPR proteins. In plastids, both MORF proteins are required for complete editing at almost all sites, suggesting a heterodimeric complex. In yeast two-hybrid and pull-down assays, MORF proteins can connect to form hetero- and homodimers. Furthermore, MORF proteins interact selectively with PPR proteins, establishing a more complex editosome in plant organelles than previously thought.
Asunto(s)
Proteínas de Arabidopsis/metabolismo , Arabidopsis/genética , Mitocondrias/genética , Familia de Multigenes , Plastidios/genética , Edición de ARN/genética , Proteínas de Arabidopsis/química , Proteínas de Arabidopsis/genética , Secuencia de Bases , Datos de Secuencia Molecular , Mutación/genética , Unión Proteica , Seudogenes/genética , Secuencias Repetitivas de Aminoácido , Técnicas del Sistema de Dos HíbridosRESUMEN
The facilitators for specific cytosine-to-uridine RNA-editing events in plant mitochondria and plastids are pentatricopeptide repeat (PPR)-containing proteins with specific additional C-terminal domains. Here we report the related PPR proteins mitochondrial editing factor 8 (MEF8) and MEF8S with only five such repeats each to be both involved in RNA editing at the same two sites in mitochondria of Arabidopsis thaliana. Mutants of MEF8 show diminished editing in leaves but not in pollen, whereas mutants of the related protein MEF8S show reduced RNA editing in pollen but not in leaves. Overexpressed MEF8 or MEF8S both increase editing at the two target sites in a mef8 mutant. Double mutants of MEF8 and MEF8S are not viable although both identified target sites are in mRNAs for nonessential proteins. This suggests that MEF8 and MEF8S may have other essential functions beyond these two editing sites in complex I mRNAs.
Asunto(s)
Proteínas de Arabidopsis/genética , Arabidopsis/genética , Edición de ARN , ARN de Planta/genética , ARN/genética , Secuencia de Aminoácidos , Arabidopsis/metabolismo , Proteínas de Arabidopsis/metabolismo , Secuencia de Bases , Sitios de Unión/genética , ADN Bacteriano/genética , Genes Letales/genética , Proteínas Mitocondriales/genética , Proteínas Mitocondriales/metabolismo , Datos de Secuencia Molecular , Mutagénesis Insercional , Mutación , Hojas de la Planta/genética , Hojas de la Planta/metabolismo , Polen/genética , Polen/metabolismo , ARN/metabolismo , ARN Mitocondrial , ARN de Planta/metabolismo , Homología de Secuencia de Aminoácido , Homología de Secuencia de Ácido NucleicoRESUMEN
A forwards genetic screen of a chemically mutated plant population identified mitochondrial RNA editing factor 10 (MEF10) in Arabidopsis thaliana. MEF10 is a trans-factor required specifically for the C to U editing of site nad2-842. The MEF10 protein is characterized by a stretch of pentatricopeptide repeats (PPR) and a C-terminal extension domain ending with the amino acids DYW. Editing is lost in mutant plants but is recovered by transgenic introduction of an intact MEF10 gene. The MEF10 protein interacts with multiple organellar RNA editing factor 8 (MORF8) but not with other mitochondrial MORF proteins in yeast two hybrid assays. These results support the model that specific combinations of MORF and MEF proteins are involved in RNA editing in plant mitochondria.
Asunto(s)
Proteínas de Arabidopsis/metabolismo , Arabidopsis/genética , Arabidopsis/metabolismo , Mitocondrias/genética , Proteínas Mitocondriales/metabolismo , Edición de ARN/genética , Secuencia de Aminoácidos , Proteínas de Arabidopsis/química , Proteínas de Arabidopsis/genética , Secuencia de Bases , Secuencia Conservada , Ecotipo , Metanosulfonato de Etilo , Genes de Plantas/genética , Proteínas Mitocondriales/química , Proteínas Mitocondriales/genética , Datos de Secuencia Molecular , Mutación/genética , Fenotipo , Unión Proteica/genética , Estructura Terciaria de Proteína , ARN Mensajero/genética , ARN Mensajero/metabolismo , Homología de Secuencia de Aminoácido , Técnicas del Sistema de Dos Híbridos , Uridina/metabolismoRESUMEN
Mitochondrial RNA editing factor 12 (MEF12) was identified in a screen for editing defects of a chemically mutated plant population in Arabidopsis thaliana. The MEF12 editing protein is required for the C to U change of nucleotide nad5-374. The MEF12 polypeptide is characterized by an exceptionally long stretch of 25 pentatricopeptide repeats (PPR) and a C-terminal extension domain. Editing is lost in mutant plants with a stop codon in the extending element. A T-DNA insertion substituting the 10 C-terminal amino acids of the extension domain reduces RNA editing to about 20% at the target site in a mutant plant. These results support the importance of the full-length extension module for functional RNA editing in plant mitochondria.
Asunto(s)
Proteínas de Arabidopsis/metabolismo , Arabidopsis/genética , Edición de ARN , Proteínas de Unión al ARN/metabolismo , Proteínas de Arabidopsis/genética , Regulación de la Expresión Génica de las Plantas , Mutación , Estructura Terciaria de Proteína , ARN/metabolismo , ARN Mitocondrial , Proteínas de Unión al ARN/genéticaRESUMEN
In plants, RNA editing is observed in mitochondria and plastids, changing selected C nucleotides into Us in both organelles. We here identify the PPR (pentatricopeptide repeat) protein MEF3 (mitochondrial editing factor 3) of the E domain PPR subclass by genetic mapping of a variation between ecotypes Columbia (Col) and Landsberg erecta (Ler) in Arabidopsis thaliana to be required for a specific RNA editing event in mitochondria. The Ler variant of MEF3 differs from Col in two amino acids in repeats 9 and 10, which reduce RNA editing levels at site atp4-89 to about 50% in Ler. In a T-DNA insertion line, editing at this site is completely lost. In Vitis vinifera the gene most similar to MEF3 continues into a DYW extension beyond the common E domain. Complementation assays with various combinations of PPR and E domains from the vine and A. thaliana proteins show that the vine E region can substitute for the A. thaliana E region with or without the DYW domain. These findings suggest that the additional DYW domain does not disturb the MEF3 protein function in mitochondrial RNA editing in A. thaliana.
Asunto(s)
Proteínas de Arabidopsis/metabolismo , Arabidopsis/genética , Mitocondrias/metabolismo , Edición de ARN , ARN de Planta/metabolismo , Arabidopsis/metabolismo , Proteínas de Arabidopsis/genética , Secuencia de Bases , Mapeo Cromosómico , ADN Bacteriano , Ecotipo , Prueba de Complementación Genética , Mitocondrias/genética , Datos de Secuencia Molecular , Mutagénesis Insercional , Dominios y Motivos de Interacción de ProteínasRESUMEN
In plant mitochondria and plastids, RNA editing alters about 400 and about 35 C nucleotides into Us, respectively. Four of these RNA editing events in plant mitochondria specifically require the PPR protein MEF7, characterized by E and DYW extension domains. The gene for MEF7 was identified by genomic mapping of the locus mutated in plants from EMS treated seeds. The SNaPshot screen of the mutant plant population identified two independent EMS mutants with the same editing defects as a corresponding T-DNA insertion line of the MEF7 gene. Although the amino acid codons introduced by the editing events are conserved throughout flowering plants, even the combined failure of four editing events does not impair the growth efficiency of the mutant plants. Five nucleotides are conserved between the four affected editing sites, but are not sufficient for specific recognition by MEF7 since they are also present at three other sites which are unaffected in the mutants.
Asunto(s)
Proteínas de Arabidopsis/metabolismo , Arabidopsis/genética , Arabidopsis/metabolismo , Mitocondrias/metabolismo , Edición de ARN , Proteínas de Unión al ARN/metabolismo , Secuencia de Aminoácidos , Proteínas de Arabidopsis/química , Proteínas de Arabidopsis/genética , Secuencia de Bases , Secuencia Conservada , Regulación de la Expresión Génica de las Plantas , Mitocondrias/genética , Datos de Secuencia Molecular , Mutación , Fenotipo , Proteínas de Unión al ARN/genética , Alineación de SecuenciaRESUMEN
RNA editing in flowering plant mitochondria post-transcriptionally alters several hundred nucleotides from C to U, mostly in mRNAs. Several factors required for specific RNA-editing events in plant mitochondria and plastids have been identified, all of them PPR proteins of the PLS subclass with a C-terminal E-domain and about half also with an additional DYW domain. Based on this information, we here probe the connection between E-PPR proteins and RNA editing in plant mitochondria. We initiated a reverse genetics screen of T-DNA insertion lines in Arabidopsis thaliana and investigated 58 of the 150 E-PPR-coding genes for a function in RNA editing. Six genes were identified to be involved in mitochondrial RNA editing at specific sites. Homozygous mutants of the five genes MEF18-MEF22 display no gross disturbance in their growth or development patterns, suggesting that the editing sites affected are not crucial at least in the greenhouse. These results show that a considerable percentage of the E-PPR proteins are involved in the functional processing of site-specific RNA editing in plant mitochondria.
Asunto(s)
Proteínas de Arabidopsis/metabolismo , Arabidopsis/metabolismo , Mitocondrias/metabolismo , Proteínas Mitocondriales/metabolismo , Edición de ARN/fisiología , ARN de Planta/metabolismo , Arabidopsis/genética , Proteínas de Arabidopsis/genética , ADN Mitocondrial/genética , ADN Mitocondrial/metabolismo , ADN de Plantas/genética , ADN de Plantas/metabolismo , Mitocondrias/genética , Proteínas Mitocondriales/genética , Mutación , Estructura Terciaria de Proteína , ARN de Planta/genéticaRESUMEN
Post-transcriptional RNA editing in flowering plant mitochondria alters several hundred nucleotides from cytidine to uridine, mostly in mRNAs. To characterize the factors involved in RNA editing in plant mitochondria, we initiated a screen for nuclear mutants defective in RNA editing at specific sites. Here we identify the nuclear-encoded gene MEF11, which is involved in RNA editing of the three sites cox3-422, nad4-124 and ccb203-344 in Arabidopsis thaliana. A T-DNA insertion line of this gene was previously characterized as showing enhanced tolerance to the compound lovastatin, an inhibitor of the mevalonate pathway of isoprenoid biosynthesis. The mef11-1 mutant described here shows similar tolerance to lovastatin. Identification of the function of the MEF11 protein in site-specific mitochondrial RNA editing suggests indirect effects of retrograde signalling from mitochondria to the cytoplasm to evoke alteration of the mevalonate pathway. The editing sites cox3-422 and ccb203-344 each alter amino acids that are conserved in the respective proteins, while the nad4-124 site is silent. The single amino acid change in the mef11-1 mutant occurs in the second pentatricopeptide repeat, suggesting that this motif is required for site-specific RNA editing.
Asunto(s)
Proteínas de Arabidopsis/metabolismo , Arabidopsis/metabolismo , Mitocondrias/metabolismo , Edición de ARN , Proteínas de Unión al ARN/metabolismo , Secuencia de Aminoácidos , Arabidopsis/química , Arabidopsis/genética , Proteínas de Arabidopsis/química , Proteínas de Arabidopsis/genética , Secuencia de Bases , Secuencia Conservada , ADN Bacteriano/genética , Regulación de la Expresión Génica de las Plantas , Datos de Secuencia Molecular , Mutación , Proteínas de Unión al ARN/química , Proteínas de Unión al ARN/genética , Alineación de SecuenciaRESUMEN
RNA editing in flowering plant mitochondria targets several hundred C nucleotides mostly in mRNAs to be altered to U. Several nuclear encoded genes have been recently identified predominantly in Arabidopsis thaliana which code for proteins involved in specific RNA editing events in plastids or mitochondria. These nuclear genes code for proteins characterized by a stretch of 4-20 repeats of 34-36 amino acids each, accordingly classified as pentatricopeptide repeat (PPR) proteins. These repeats most likely participate in recognizing and binding the specific nucleotide motifs around editing sites which have been defined as essential cis-elements. All of the RNA editing PPR proteins contain at their C-termini an extension of as yet unclear function, the E domain, and some of these are further extended by another domain which terminates with the triplet DYW. While the E domain seems to be always required for their function in RNA editing, the DYW domain can sometimes be removed. At some editing sites a given PPR protein seems to be required, while at others their function can at least partially be compensated by presumably other PPR proteins. These observations suggest that the PPR proteins may act in a complex network to define and to target RNA editing sites.
Asunto(s)
Proteínas de Arabidopsis/metabolismo , Arabidopsis/genética , Edición de ARN , ARN Mensajero/metabolismo , Aminoácidos/metabolismo , Arabidopsis/metabolismo , Dominio Catalítico , Mitocondrias/genética , Mitocondrias/metabolismo , Proteínas Mitocondriales/metabolismo , Mutagénesis Insercional , Protoplastos/metabolismo , ARN/genética , ARN/metabolismo , ARN Mitocondrial , ARN de Planta/metabolismo , Proteínas de Unión al ARN/metabolismoRESUMEN
RNA editosomes selectively deaminate cytidines to uridines in plant organellar transcripts-mostly to restore protein functionality and consequently facilitate mitochondrial and chloroplast function. The RNA editosomal pentatricopeptide repeat proteins serve target RNA recognition, whereas the intensively studied DYW domain elicits catalysis. Here we present structures and functional data of a DYW domain in an inactive ground state and activated. DYW domains harbour a cytidine deaminase fold and a C-terminal DYW motif, with catalytic and structural zinc atoms, respectively. A conserved gating domain within the deaminase fold regulates the active site sterically and mechanistically in a process that we termed gated zinc shutter. Based on the structures, an autoinhibited ground state and its activation are cross-validated by RNA editing assays and differential scanning fluorimetry. We anticipate that, in vivo, the framework of an active plant RNA editosome triggers the release of DYW autoinhibition to ensure a controlled and coordinated cytidine deamination playing a key role in mitochondrial and chloroplast homeostasis.
RESUMEN
C to U editing is one of the post-transcriptional steps which are required for the proper expression of chloroplast and mitochondrial genes in plants. It depends on several proteins acting together which include the PLS-class pentatricopeptide repeat proteins (PPR). DYW2 was recently shown to be required for the editing of many sites in both organelles. In particular almost all the sites associated with the E+ subfamily of PPR proteins are depending on DYW2, suggesting that DYW2 is required for the function of E+-type PPR proteins. Here we strengthened this link by identifying 16 major editing sites controlled by 3 PPR proteins: OTP90, a DYW-type PPR and PGN and MEF37, 2 E+-type PPR proteins. A re-analysis of the DYW2 editotype showed that the 49 sites known to be associated with the 18 characterized E+-type PPR proteins all depend on DYW2. Considering only the 288 DYW2-dependent editing sites as potential E+-type PPR sites, instead of the 795 known editing sites, improves the performances of binding predictions systems based on the PPR code for E+-type PPR proteins. However, it does not compensate for poor binding predictions.
RESUMEN
RNA editing changes more than 400 cytidines to uridines in the mRNAs of mitochondria in flowering plants. In other plants such as ferns and mosses, RNA editing reactions changing C to U and U to C are observed at almost equal frequencies. Development of transfection systems with isolated mitochondria and of in vitro systems with extracts from mitochondria has considerably improved our understanding of the recognition of specific editing sites in the last few years. These assays have also yielded information about the biochemical parameters, but the enzymes involved have not yet been identified. Here we summarize our present understanding of the process of RNA editing in flowering plant mitochondria.
Asunto(s)
Regulación de la Expresión Génica de las Plantas , Mitocondrias/genética , Plantas/genética , Edición de ARN/fisiología , Brassica/genética , Ligasas de Carbono-Nitrógeno/fisiología , Citidina Desaminasa/fisiología , Desaminación , Evolución Molecular , Genoma Mitocondrial/fisiología , Genoma de Planta/fisiología , Pisum sativum/genética , ARN Helicasas/fisiología , Transaminasas/fisiología , Triticum/genética , Zea mays/genéticaRESUMEN
Most RNA editing sites in flowering plant mitochondria are located in coding regions of mRNAs and are usually essential for correct gene expression. Although accordingly little variation should be tolerated, editing sites appear and disappear even between closely related flowering plant species. To investigate whether such editing site variations also occur within species, we analyzed 379 RNA editing sites in the three ecotypes Columbia, Landsberg erecta and C24 of Arabidopsis thaliana. While all editing sites as such are conserved, we identify seven RNA editing sites with 40-60% differences in effective editing between individual ecotypes. These quantitative variations show that the extent of RNA editing in plant mitochondria is very flexible and can change even more rapidly than the evolution of species. The ecotype-specific variations of the RNA editing extent are Mendelian-inherited and can now be used to follow and identify the nuclear loci responsible for these RNA editing phenotypes.
Asunto(s)
Arabidopsis/genética , Mitocondrias/genética , Edición de ARN/genética , ARN de Planta/genética , Flores/genética , Hojas de la Planta/genéticaRESUMEN
RNA editing in flowering plant mitochondria is investigated by in vitro assays. These cauliflower mitochondrial lysates require added NTP or dNTP. We have now resolved the reason for this requirement to be the inhibition of the RNA binding activity of the glutamate dehydrogenases (GDH). Both GDH1 and GDH2 were identified in RNA-protein cross-links. The inhibition of in vitro RNA editing by GDH is confirmed by the ability of the GDH-specific herbicide phosphinothricin to substitute for NTP. NADH and NADPH, but not NAD or NADP, can also replace NTP, suggesting that the NAD(P)H-binding-pocket configuration of the GDH contacts the RNA. RNA editing in plant mitochondria is thus intrinsically independent of added energy in the form of NTP.
Asunto(s)
Brassica/genética , Mitocondrias/genética , Edición de ARN , ARN/genética , Adenosina Trifosfato/metabolismo , Adenosina Trifosfato/farmacología , Aminobutiratos/farmacología , Citidina Trifosfato/metabolismo , Citidina Trifosfato/farmacología , Glutamato Deshidrogenasa/antagonistas & inhibidores , Glutamato Deshidrogenasa/metabolismo , Glutamato Deshidrogenasa (NADP+)/antagonistas & inhibidores , Glutamato Deshidrogenasa (NADP+)/metabolismo , Isoenzimas/antagonistas & inhibidores , Isoenzimas/metabolismo , Mitocondrias/efectos de los fármacos , Mitocondrias/metabolismo , Proteínas Mitocondriales/metabolismo , ATPasas de Translocación de Protón Mitocondriales/genética , NAD/metabolismo , NAD/farmacología , Unión Proteica , ARN/metabolismo , ARN Mitocondrial , Reacción en Cadena de la Polimerasa de Transcriptasa InversaRESUMEN
RNA editing in flowering plant mitochondria alters numerous C nucleotides in a given mRNA molecule to U residues. To investigate whether neighbouring editing sites can influence each other we analyzed in vitro RNA editing of two sites spaced 30 nt apart. Deletion and competition experiments show that these two sites carry independent essential specificity determinants in the respective upstream 20-30 nucleotides. However, deletion of a an upstream sequence region promoting editing of the upstream site concomitantly decreases RNA editing of the second site 50-70 nucleotides downstream. This result suggests that supporting cis-/trans-interactions can be effective over larger distances and can affect more than one editing event.
Asunto(s)
Brassica/fisiología , Flores/fisiología , Mitocondrias/fisiología , Edición de ARN/fisiología , Eliminación de Secuencia , Citidina/genética , Uridina/genéticaRESUMEN
RNA editing sites in plant mitochondria and plastids are addressed by pentatricopeptide repeat (PPR) proteins with E or E and DYW domains, which recognize a specific nucleotide motif upstream of the edited nucleotide. In addition, some sites require MORF proteins for efficient RNA editing. Here, we assign the novel E domain-containing PPR protein, MEF13, as being required for editing at eight sites in Arabidopsis thaliana. A SNP in ecotype C24 altering the editing level at only one of the eight target sites was located by genomic mapping. An EMS mutant allele of the gene for MEF13 was identified in a SNaPshot screen of a mutated plant population. At all eight target sites of MEF13, editing levels are reduced in both morf3 and morf8 mutants, but at only one site in morf1 mutants, suggesting that specific MEF13-MORF interactions are required. Yeast two-hybrid analyses detect solid connections of MEF13 with MORF1 and weak contact with MORF3 proteins. Yeast three-hybrid (Y3H) analysis shows that the presence of MORF8 enhances the connection between MEF13 and MORF3, suggesting that a MORF3-MORF8 heteromer may form stably or transiently to establish interaction with MEF13.
Asunto(s)
Proteínas de Arabidopsis/genética , Arabidopsis/genética , Edición de ARN/genética , Mitocondrias/genética , Mutación , ARN Mensajero/genética , ARN Mitocondrial , ARN de Planta/genéticaRESUMEN
RNA editing changes several hundred cytidines to uridines in the mRNAs of mitochondria in flowering plants. The target cytidines are identified by a subtype of PPR proteins characterized by tandem modules which each binds with a specific upstream nucleotide. Recent progress in correlating repeat structures with nucleotide identities allows to predict and identify target sites in mitochondrial RNAs. Additional proteins have been found to play a role in RNA editing; their precise function still needs to be elucidated. The enzymatic activity performing the C to U reaction may reside in the C-terminal DYW extensions of the PPR proteins; however, this still needs to be proven. Here we update recent progress in understanding RNA editing in flowering plant mitochondria.
Asunto(s)
Mitocondrias/enzimología , Mitocondrias/metabolismo , Proteínas Mitocondriales/metabolismo , Proteínas de Plantas/metabolismo , Plantas/metabolismo , Edición de ARN , ARN/metabolismo , Citidina/metabolismo , Plantas/enzimología , Plantas/genética , ARN Mensajero/metabolismo , ARN Mitocondrial , Uridina/metabolismoRESUMEN
We here identify the PPR protein MEF14 of the DYW subclass as a specific trans-factor required for C to U editing of site matR-1895 by genetic mapping of an EMS induced editing mutant in Arabidopsis thaliana. The wild type Col MEF14 gene complements mutant protoplasts. A T-DNA insertion in the MEF14 gene abolishes detectable editing at the matR-1895 site. Lack of RNA editing at the matR-1895 site does not alter the level of mature and precursor nad1 mRNA molecules. Such RNA editing mutants can be used to analyse the function of genes like this maturase related reading frame in plant mitochondria.
Asunto(s)
Proteínas de Arabidopsis/metabolismo , Arabidopsis/metabolismo , Mitocondrias/metabolismo , Proteínas Mitocondriales/metabolismo , Edición de ARN , Transactivadores/metabolismo , Secuencia de Aminoácidos , Proteínas de Arabidopsis/genética , Bases de Datos de Proteínas , Defensinas/genética , Defensinas/metabolismo , Endorribonucleasas , Metanosulfonato de Etilo/farmacología , Sitios Genéticos , Proteínas Mitocondriales/genética , Datos de Secuencia Molecular , Mutágenos/farmacología , Proteínas Mutantes/metabolismo , Nucleotidiltransferasas , Fenotipo , Hojas de la Planta/metabolismo , ARN Mensajero , Alineación de Secuencia , Homología de Secuencia de Aminoácido , Especificidad por Sustrato , Transactivadores/genéticaRESUMEN
RNA editing is a post-transcriptional molecular process whereby the information in a genetic message is modified from that in the corresponding DNA template by means of nucleotide substitutions, insertions and/or deletions. It occurs mostly in organelles by clade-specific diverse and unrelated biochemical mechanisms. RNA editing events have been annotated in primary databases as GenBank and at more sophisticated level in the specialized databases REDIdb, dbRES and EdRNA. At present, REDIdb is the only freely available database that focuses on the organellar RNA editing process and annotates each editing modification in its biological context. Here we present an updated and upgraded release of REDIdb with a web-interface refurbished with graphical and computational facilities that improve RNA editing investigations. Details of the REDIdb features and novelties are illustrated and compared to other RNA editing databases. REDIdb is freely queried at http://biologia.unical.it/py_script/REDIdb/.