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1.
BMC Vet Res ; 18(1): 143, 2022 Apr 19.
Artículo en Inglés | MEDLINE | ID: mdl-35439995

RESUMEN

BACKGROUND: Neuromedin U (NMU) plays an important role in activating the group 2 innate lymphoid cells (ILC2s) and initiating the host's anti-parasitic immune responses. It is aimed to explore the distribution characteristics of NMU in the sheep small intestine and the influence of Moniezia benedeni infection on them. In the present study, the pET-28a-NMU recombinant plasmids were constructed, and Escherichia coli. BL21 (DE3) were induced to express the recombinant protein. And then, the rabbit anti-sheep NMU polyclonal antibody was prepared and immunofluorescence staining was performed with it. The expression levels of NMU in the intestine of normal and Moniezia benedeni-infected sheep were detected by ELISA. RESULTS: The results showed that the molecular weight of the obtained NMU recombinant protein was consistent with the expected molecular (13 kDa) and it was expressed in the form of inclusion body. The titer and specificity of obtained rabbit anti-sheep NMU polyclonal antibody were good. The results of immunofluorescence analysis showed that the nerve fibers which specifically expressed NMU mainly extended from the ganglion in the submucosal to lamina propria (LP) in the sheep small intestine, and the expression level was relatively high; especially on the nerve fibers of LP around the intestinal glands. The expression levels were gradually increased from the duodenum to the ileum, and the levels in the jejunum and ileum were significantly higher than that in the duodenum (P < 0.05). In addition, scattered NMU positive cells were distributed in the epithelium of the jejunal crypts. Moniezia benedeni infection increased the expression of NMU in each intestinal segment, especially in the jejunum and ileum there were significant increase (P < 0.05). CONCLUSIONS: It was suggested that Moniezia benedeni infection could be detected by the high expression of NMU in sheep enteric nervous, and which laid the foundation for further studies on whether NMU exerts anti-parasitic immunity by activating ILC2s. In addition, NMU was expressed in some intestinal gland epitheliums, which also provided a basis for studying its roles in regulation of the immune homeostasis. The present study laid the foundation for further revealing the molecular mechanism of sheep's neural-immune interaction network perceiving the colacobiosis of parasites.


Asunto(s)
Cestodos , Inmunidad Innata , Animales , Inmunidad Innata/genética , Intestino Delgado , Linfocitos , Neuropéptidos , Conejos , Proteínas Recombinantes , Ovinos , Oveja Doméstica
2.
Appl Opt ; 61(10): 2898-2902, 2022 Apr 01.
Artículo en Inglés | MEDLINE | ID: mdl-35471367

RESUMEN

We present a kilowatt-level quasi-continuous-wave (QCW) cryogenically cooled 946-nm slab laser oscillator for the first time, to the best of our knowledge. The laser system is based on a double-face-pumped large-size single-slab Nd:YAG design, delivering a record-high average power of 1.06 kW without additional amplification. This laser oscillator operates at repetition rate of 400 Hz with a pulse duration of 175 µs, resulting in a single pulse energy of 2.65 J. To the best of our knowledge, these results represent the highest output power and pulse energy for any all-solid-state 946-nm laser ever reported to date. Our scheme paves a new path for the development of the compact high-power solid-state 946-nm laser.

3.
Neoplasma ; 69(4): 841-858, 2022 Jul.
Artículo en Inglés | MEDLINE | ID: mdl-35532293

RESUMEN

The present study aimed to investigate LINC00278 expression in laryngeal squamous cell carcinoma (LSCC) and its involvement in the process of proliferation, migration, and invasion, providing a rationale for mining potential diagnostic and therapeutic targets of LSCC. Univariate and multivariate Cox regression analyses were performed to identify optimal prognostic lncRNAs. MTS, colony formation, wound healing, and Transwell invasion assays were used to determine the effects of LINC00278 overexpression on the proliferation, migration, and invasion of cancer cells. The expressions of signaling pathway-related proteins and epithelial-mesenchymal transition (EMT) marker proteins were detected using western blot. The chromatin immunoprecipitation (ChIP) and dual-luciferase reporter assays were performed to demonstrate the binding of ETS proto-oncogene 1, transcription factor (ETS1), and LINC00278 promoter region. The molecular targets of LINC00278 were identified by RNA sequencing analysis and co-expression analysis. Kaplan-Meier analysis and CIBERSORT algorithm were used to analyze survival and immune cell infiltration based on LINC00278, COL4A1, and COL4A2. Multivariate Cox regression was used to establish a six-gene prognostic model. LINC00278 expression was low in LSCC tissues, and it was significantly associated with the TNM (tumors/nodes/metastases) stage (p<0.001), lymphatic metastasis (p<0.01), and pathological differentiation (p<0.01). LINC00278 overexpression significantly reduced LSCC cell proliferation, migration, and invasion in TU686, TU177, and AMC-HN-8 cell lines. E-cadherin protein expression was increased, while N-cadherin, Vimentin, Zeb1, and Snail protein expression was decreased in the LINC00278 group, compared to the pcDNA3.1 group. Additionally, in AMC-HN-8 and FaDu cell lines, the LINC00278-treated group had significantly lower p-AKT and p-mTOR protein levels than the control group. ETS1 is a direct transcriptional regulator of the LINC00278 gene based on luciferase reporter assays and ChIP experiments. Western blot analysis demonstrated that high LINC00278 expression inhibited both ETS1 expression and phosphorylation. COL4A1/COL4A2 were identified as potential downstream targets of LINC00278. Meanwhile, the LINC00278/COL4A1/COL4A2-dominated low-risk group showed higher antigen-presenting activity and a higher immune score than the high-risk group. The findings indicated that ETS1 upregulated LINC00278 expression on the Y chromosome, which in turn inhibited LSCC growth in vivo and in vitro by inhibiting the AKT/mTOR signaling pathway via downregulation of COL4A1/COL4A2.


Asunto(s)
Neoplasias de Cabeza y Cuello , Neoplasias Laríngeas , Línea Celular Tumoral , Movimiento Celular , Proliferación Celular , Colágeno Tipo IV/genética , Colágeno Tipo IV/metabolismo , Transición Epitelial-Mesenquimal , Retroalimentación , Regulación Neoplásica de la Expresión Génica , Neoplasias de Cabeza y Cuello/genética , Humanos , Neoplasias Laríngeas/genética , Neoplasias Laríngeas/patología , Proteína Proto-Oncogénica c-ets-1/genética , Proteína Proto-Oncogénica c-ets-1/metabolismo , Proteínas Proto-Oncogénicas c-akt/metabolismo , Carcinoma de Células Escamosas de Cabeza y Cuello/genética
4.
Biochem Biophys Res Commun ; 557: 69-76, 2021 06 11.
Artículo en Inglés | MEDLINE | ID: mdl-33862462

RESUMEN

Remifentanil is a potent, short-acting opioid analgesic drug that can protect tissues from ischemia and reperfusion injury though anti-inflammatory effects. However, the utility of remifentanil in liver regeneration after hepatectomy is not known. Using a 70% hepatectomy mouse model (PHx), we found that preconditioning animals with 4 µg/kg remifentanil enhanced liver regeneration through supporting hepatocyte proliferation but not through anti-inflammatory effects. These effects were also phenocopied in vitro where 40 mM remifentanil promoted the proliferation of primary mouse hepatocyte cultures. We further identified that remifentanil treatment increased the expression of ß-arrestin 2 in vivo and in vitro. Demonstrating specificity, remifentanil preconditioning failed to promote liver regeneration in liver-specific ß-arrestin 2 knockout (CKO) mice subjected to PHx. While remifentanil increased the expression of activated (phosphorylated)-ERK and cyclin D1 in PHx livers, their levels were not significantly changed in remifentanil-treated CKO mice nor in WT mice pretreated with the ERK inhibitor U0126. Our findings suggest that remifentanil promotes liver regeneration via upregulation of a ß-arrestin 2/ERK/cyclin D1 axis, with implications for improving regeneration process after hepatectomy.


Asunto(s)
Ciclina D1/metabolismo , Regeneración Hepática , Sistema de Señalización de MAP Quinasas/efectos de los fármacos , Remifentanilo/farmacología , Daño por Reperfusión/terapia , Arrestina beta 2/metabolismo , Analgésicos Opioides/farmacología , Animales , Proliferación Celular/fisiología , Células Cultivadas , Modelos Animales de Enfermedad , Hepatectomía , Hepatocitos/efectos de los fármacos , Hepatocitos/metabolismo , Masculino , Ratones , Ratones Endogámicos C57BL , Ratones Noqueados , Ratones Transgénicos , Daño por Reperfusión/metabolismo , Daño por Reperfusión/patología , Regulación hacia Arriba
5.
Neoplasma ; 68(6): 1212-1224, 2021 Nov.
Artículo en Inglés | MEDLINE | ID: mdl-34585589

RESUMEN

This study aimed to elucidate the potential genes of the matrix metalloproteinase (MMP) family, responsible for the progression of laryngeal squamous cell carcinoma (LSCC). Besides, we ascertained the changes in common malignant behaviors in vitro by knocking down MMP1. TCGA, GEO, Oncomine, and microarray data were conducted to analyze the expression levels of MMPs and to find tissue-specific genes in LSCC. Univariate and multivariate Cox regression analyses were established in the construction of a prognostic model based on expression profiles and clinical information of LSCC in TCGA. We then comprehensively analyzed survival, co-expression network, and immune infiltration based on a prognostic model by Kaplan-Meier analysis, WGCNA, and CIBERSORT. Thereafter, qRT-PCR, proliferation, Transwell, and wound-healing assays were used to assess the accuracy of the bioinformatics data. A total of seven genes in the MMP family were identified as differentially expressed genes (DEGs) by integrating three public databases and microarray data. Additionally, multivariate Cox regression was used to establish a four-gene (MMP1/3/8/10) prognostic model, which exhibited a better predictive accuracy than the TNM (tumors/nodes/metastases) based model. The prognostic model was related to plasma cells, CD8+ T cells, follicular helper T cells, resting NK cells, and M0 macrophages infiltration. The expression of MMP1, MMP3, and MMP10 was the highest in head and neck squamous cell carcinoma (HNSC) compared to other cancer in the Oncomine and GEPIA dataset. Further, MMP1 demonstrated significant upregulation in 40 paired LSCC tissues. Eventually, MMP1 downregulation inhibited cell viability, colony formation, and cell migration in TU686 and FaDu cells. Our findings suggest that the four-gene signature might be associated with the prognosis. Further, we revealed that MMP1 is a pivotal biomarker for the biotherapy and prognostic evaluation of patients with LSCC.


Asunto(s)
Carcinoma de Células Escamosas , Neoplasias de Cabeza y Cuello , Neoplasias Laríngeas , Biomarcadores de Tumor/genética , Carcinoma de Células Escamosas/genética , Regulación Neoplásica de la Expresión Génica , Humanos , Neoplasias Laríngeas/genética , Pronóstico , Carcinoma de Células Escamosas de Cabeza y Cuello
6.
Appl Opt ; 59(2): 459-462, 2020 Jan 10.
Artículo en Inglés | MEDLINE | ID: mdl-32225331

RESUMEN

A void-free bonding technique was demonstrated for a large slab Nd: YAG crystal with a bonding surface dimension of ∼160mm×70mm. By using the novel fluxless oxide layer removal technology, the indium-oxide barrier problem was resolved. With the help of electrochemical-polished indium solder and a plasma-cleaned heat sink, the solderability of the indium was enhanced; in particular, the contact angle of the solder was improved from 51° to 31°. With the largest-bonding-size slab, a single-slab laser created a maximum output power of 7.3 kW under an absorbed pump power of 12.8 kW, corresponding to an optical to optical efficiency of 57% and a slope conversion of 67.8%. By detecting the wavefront of the interferometer before and after bonding, the RMS of wavefront was 0.192λ and 0.434λ (λ=633nm), respectively. To the best of our knowledge, this is the largest void-free bonding size for a laser slab and the highest output power achieved from a single-slab crystal laser oscillator.

7.
Plant Mol Biol ; 91(3): 241-56, 2016 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-26936070

RESUMEN

Limonium bicolor, a typical recretohalophyte that lives in saline environments, excretes excessive salt to the environment through epidermal salt glands to avoid salt stress. The aim of this study was to screen for L. bicolor genes involved in salt secretion by high-throughput RNA sequencing. We established the experimental procedure of salt secretion using detached mature leaves, in which the optimal salt concentration was determined as 200 mM NaCl. The detached salt secretion system combined with Illumina deep sequencing were applied. In total, 27,311 genes were annotated using an L. bicolor database, and 2040 of these genes were differentially expressed, of which 744 were up-regulated and 1260 were down-regulated with the NaCl versus the control treatment. A gene ontology enrichment analysis indicated that genes related to ion transport, vesicles, reactive oxygen species scavenging, the abscisic acid-dependent signaling pathway and transcription factors were found to be highly expressed under NaCl treatment. We found that 102 of these genes were likely to be involved in salt secretion, which was confirmed using salt-secretion mutants. The present study identifies the candidate genes in the L. bicolor salt gland that are highly associated with salt secretion. In addition, a salt-transporting pathway is presented to explain how Na(+) is excreted by the salt gland in L. bicolor. These findings will shed light on the molecular mechanism of salt secretion from the salt glands of plants.


Asunto(s)
Hojas de la Planta/genética , Plumbaginaceae/genética , Plantas Tolerantes a la Sal/genética , Transcriptoma/genética , Regulación de la Expresión Génica de las Plantas/efectos de los fármacos , Regulación de la Expresión Génica de las Plantas/fisiología , Genes de Plantas/genética , Genes de Plantas/fisiología , Hojas de la Planta/efectos de los fármacos , Hojas de la Planta/fisiología , Plumbaginaceae/efectos de los fármacos , Plumbaginaceae/fisiología , Tolerancia a la Sal/efectos de los fármacos , Tolerancia a la Sal/genética , Tolerancia a la Sal/fisiología , Plantas Tolerantes a la Sal/efectos de los fármacos , Plantas Tolerantes a la Sal/fisiología , Cloruro de Sodio/farmacología , Transcriptoma/fisiología
8.
Apoptosis ; 21(4): 489-501, 2016 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-26801320

RESUMEN

It has been previously reported that cisplatin is a well-known anticancer drug being used against a wide range of malignancies including head and neck, ovarian and non-small cell lung carcinoma, and demonstrated its anticancer activity by reacting with DNA or changing cell structure, immune response, reactive oxygen species level (ROS). In this research we proved that cisplatin induced cell injuries and heme oxygenase-1 (HO-1) expression in laryngeal squamous cell cancer Hep-2 cells through ROS generation. The induction of HO-1 clearly protected Hep-2 cells from cisplatin-induced cell death and ROS reaction, and the inhibitor of HO-1 enhanced the cell death and ROS generation induced by cisplatin. Furthermore, the HO-1 expression induced by cisplatin was strongly inhibited by the knockdown of nuclear factor-erythroid-2-related factor-2 (Nrf-2), and the oxidative damages induced by cisplatin were significantly enhanced. Therefore, it may be concluded that the inhibition of HO-1 or the knockdown of Nrf-2 significantly enhanced cisplatin's anticancer effects on Hep-2 cells. In clinic, with the overexpression of HO-1 in laryngeal squamous cancer tissues, the combination of cisplatin with the inhibitor of HO-1 or Nrf-2 siRNA may act as a new method to the treatment of laryngeal squamous cancer.


Asunto(s)
Antineoplásicos/farmacología , Carcinoma de Células Escamosas/tratamiento farmacológico , Cisplatino/farmacología , Hemo-Oxigenasa 1/antagonistas & inhibidores , Neoplasias Laríngeas/tratamiento farmacológico , Factor 2 Relacionado con NF-E2/genética , Línea Celular Tumoral , Resistencia a Antineoplásicos , Hemo-Oxigenasa 1/metabolismo , Humanos , Factor 2 Relacionado con NF-E2/metabolismo , Oxidación-Reducción/efectos de los fármacos , Estrés Oxidativo/efectos de los fármacos , Interferencia de ARN , ARN Interferente Pequeño/genética , Especies Reactivas de Oxígeno/metabolismo
9.
Plant Cell Environ ; 38(8): 1637-57, 2015 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-25651944

RESUMEN

With the expansion of saline land worldwide, it is essential to establish a model halophyte to study the salt-tolerance mechanism. The salt glands in the epidermis of Limonium bicolor (a recretohalophyte) play a pivotal role in salt tolerance by secreting excess salts from tissues. Despite the importance of salt secretion, nothing is known about the molecular mechanisms of salt gland development. In this study, we applied RNA sequencing to profile early leaf development using five distinct developmental stages, which were quantified by successive collections of the first true leaves of L. bicolor with precise spatial and temporal resolution. Specific gene expression patterns were identified for each developmental stage. In particular, we found that genes controlling salt gland differentiation in L. bicolor may evolve in a trichome formation, which was also confirmed by mutants with increased salt gland densities. Genes involved in the special ultrastructure of salt glands were also elucidated. Twenty-six genes were proposed to participate in salt gland differentiation. Our dataset sheds light on the molecular processes underpinning salt gland development and thus represents a first step towards the bioengineering of active salt-secretion capacity in crops.


Asunto(s)
Perfilación de la Expresión Génica , Regulación del Desarrollo de la Expresión Génica , Regulación de la Expresión Génica de las Plantas , Hojas de la Planta/anatomía & histología , Hojas de la Planta/genética , Plumbaginaceae/crecimiento & desarrollo , Plumbaginaceae/genética , Calibración , Análisis por Conglomerados , Regulación hacia Abajo/genética , Ontología de Genes , Genes de Plantas , Mitocondrias/metabolismo , Modelos Biológicos , Anotación de Secuencia Molecular , Mutación/genética , Hojas de la Planta/crecimiento & desarrollo , Hojas de la Planta/ultraestructura , Estomas de Plantas/genética , Plumbaginaceae/ultraestructura , ARN Mensajero/genética , ARN Mensajero/metabolismo , Reacción en Cadena en Tiempo Real de la Polimerasa , Reproducibilidad de los Resultados , Análisis de Secuencia de ARN , Factores de Transcripción/metabolismo , Transcriptoma/genética , Tricomas/metabolismo , Regulación hacia Arriba/genética
10.
Appl Opt ; 54(16): 5011-5, 2015 Jun 01.
Artículo en Inglés | MEDLINE | ID: mdl-26192659

RESUMEN

An 8 kW level quasi-continuous-wave (QCW) face-pumped 1064 nm slab laser with high beam quality was developed by a master oscillator power amplifier (MOPA) system. A single-mode fiber seed laser was amplified by two-stage single-pass Nd:YAG rod preamplifiers and four face-pumped Nd:YAG slab amplifiers. The slab amplifiers were well designed with uniform pumping and uniform cooling for well-distributed thermal and stress. A dynamically feedbacked optical aberration compensation device was employed to correct low-order optical aberration, and the residue high-order optical aberration was corrected by an adaptive optics system. The QCW MOPA delivered up to an average power of 8.2 kW with a pulse duration of 200 µs at a repetition rate of 400 Hz. The beam quality factor was measured to be ß=3.5.

11.
Langmuir ; 30(11): 3001-9, 2014 Mar 25.
Artículo en Inglés | MEDLINE | ID: mdl-24571294

RESUMEN

The effects of three sulfonate gemini surfactants with different hydrophobic chain lengths (8, 10, and 12 carbon atoms) on the optical properties of a fluorene-based conjugated cationic polymer poly{[9,9-bis(6'-N,N,N-trimethylammonium)hexyl]-fluorene-phenylene} bromide (PFP) dissolved in DMSO-water solutions (4% v/v) or water were investigated, respectively. When surfactants with PFP dissolved in DMSO-water solutions (4% v/v) are incubated, a decrease in photoluminescence (PL) intensity and a red shift of emission maxima are obtained at low surfactant concentrations. Intriguingly, two different Stern-Volmer constants (KSV1 and KSV2) are obtained and analyzed in detail for the first time. Further increase in the surfactant concentration enhanced PL intensity, and distinct blue shifts of both absorption and emission maxima are observed. Importantly, the turning point between the emission quenching and enhancement is closely related to the hydrophobic chain length: the longer the chain length, the earlier the turning point appears. Simulation studies provide strong evidence to explain these phenomena. Surface tension measurements show more insight on the interactions between PFP and surfactant. On the contrary, no emission quenching is obtained at low surfactant concentrations for PFP dissolved in water.


Asunto(s)
Fluorenos/química , Fenómenos Ópticos , Polímeros/química , Compuestos de Amonio Cuaternario/química , Tensoactivos/química , Absorción , Dimetilsulfóxido/química , Conformación Molecular , Simulación de Dinámica Molecular , Tensión Superficial , Agua/química
12.
J Dig Dis ; 25(3): 191-199, 2024 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-38697920

RESUMEN

OBJECTIVE: To compare the detection rate and diagnostic accuracy of cardia polyps using endoscopy with blue laser imaging (BLI) and white-light imaging (WLI). METHODS: Patients were randomly divided into the BLI group and WLI group according to the endoscopic procedures. BLI followed by WLI was conducted in the BLI group, whereas WLI followed by BLI examination was conducted in the WLI group. The number, size, microstructure, and microvascular patterns of cardia polyps detected were recorded. Biopsy of the polyps was then performed. RESULTS: The detection rate of cardia polyps in the BLI group was higher than that in the WLI group (7.87% vs 4.22%, P = 0.018). The rate of overlooked lesions in the BLI group was lower than in the WLI group (0.64% vs 3.38%, P = 0.003). The diagnostic coincidence rate between magnifying BLI and histopathology was 88.16%. The sensitivity, specificity, positive predictive value and negative predictive value for the diagnosis of neoplastic lesions by magnifying endoscopy with BLI were 90.91%, 87.69%, 55.56%, and 98.28%, respectively. The most remarkable patterns for predicting inflammatory polyps were the prolonged and fine network patterns (sensitivity 71.43%, specificity 93.75%). Small round combined with honeycomb patterns were the most common among fundic gland polyps (sensitivity 80.00%, specificity 98.48%). Neoplastic lesions presented as villous or ridge-like combined with core vascular or unclear pattern for both microvascular and microstructure patterns. CONCLUSION: BLI is more effective than WLI in the detection and diagnosis of cardia polyps, and magnifying endoscopy with BLI may help diagnose such lesions.


Asunto(s)
Cardias , Estudios de Factibilidad , Neoplasias Gástricas , Humanos , Femenino , Masculino , Persona de Mediana Edad , Cardias/patología , Cardias/diagnóstico por imagen , Adulto , Neoplasias Gástricas/diagnóstico por imagen , Neoplasias Gástricas/diagnóstico , Neoplasias Gástricas/patología , Anciano , Pólipos/diagnóstico por imagen , Pólipos/diagnóstico , Gastroscopía/métodos , Sensibilidad y Especificidad , Valor Predictivo de las Pruebas , Rayos Láser
13.
Vet Parasitol ; 328: 110169, 2024 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-38520755

RESUMEN

The concentration of immunoglobulin (Ig) E is the lowest among serum Igs, but it can induces type I hypersensitivity and plays an important role in anti-parasitic infection. The present study aimed to explore the residence characteristics of IgE+ cells in the sheep small intestine and the impact of Moniezia benedeni infection on them. The recombinant plasmids pET-28a-IgE were constructed and induced and expressed in Escherichia coli. BL21 (DE3). The rabbit anti-sheep IgE polyclonal antibody was prepared using the obtained recombinant protein as antigen. Finally, the levels of IgE+ cells in the small intestine of healthy (Control group) and naturally M. benedeni-infected (Infected group) sheep were detected analyzed. The results showed that the rabbit anti-sheep IgE polyclonal antibody with good immunogenicity (titer = 1: 128000) could specifically bind to the heavy chain of natural sheep IgE. In the Control group, the IgE+ cells were mainly distributed in lamina propria of the small intestine, and the densities were significantly decreased from duodenum to ileum (P<0.05), with respective values of (4.28 cells / 104 µm2, 1.80 cells / 104 µm2, and 1.44 cells / 104 µm2 in duodenum, jejunum, and ileum. In the Infected group, IgE+ cells density were 6.26 cells / 104 µm2, 3.01 cells / 104 µm2, and 2.09 cells / 104 µm2 in duodenum, jejunum and ileum respectively, which were significantly higher in all segments compared to the Control group (P<0.05), increasing by 46.26%, 67.22% and 45.14%, respectively. In addition, compared with the Control group, the IgE protein levels were significantly increased in all intestinal segments of the Infected group (P<0.01), however, there was no significant differences among the different intestinal segments within the same group (P>0.05). The results demonstrated that M. benedeni infection could significantly increase the content of IgE and the distribution density of its secreting cells in sheep small intestine. The intestinal mucosal immune system of sheep presented obvious specificity against M. benedeni infection. This lays a good foundation for further exploring molecular mechanisms of the intestinal mucosal immune system monitoring and responding to M. benedeni infection.


Asunto(s)
Inmunoglobulina E , Intestino Delgado , Enfermedades de las Ovejas , Animales , Inmunoglobulina E/sangre , Ovinos , Enfermedades de las Ovejas/inmunología , Enfermedades de las Ovejas/parasitología , Intestino Delgado/inmunología , Intestino Delgado/parasitología , Infecciones por Cilióforos/veterinaria , Infecciones por Cilióforos/inmunología , Infecciones por Cilióforos/parasitología
14.
Nat Commun ; 15(1): 5288, 2024 Jun 20.
Artículo en Inglés | MEDLINE | ID: mdl-38902277

RESUMEN

Psoriasis is an immune-mediated skin disease associated with neurogenic inflammation, but the underlying molecular mechanism remains unclear. We demonstrate here that acid-sensing ion channel 3 (ASIC3) exacerbates psoriatic inflammation through a sensory neurogenic pathway. Global or nociceptor-specific Asic3 knockout (KO) in female mice alleviates imiquimod-induced psoriatic acanthosis and type 17 inflammation to the same extent as nociceptor ablation. However, ASIC3 is dispensable for IL-23-induced psoriatic inflammation that bypasses the need for nociceptors. Mechanistically, ASIC3 activation induces the activity-dependent release of calcitonin gene-related peptide (CGRP) from sensory neurons to promote neurogenic inflammation. Botulinum neurotoxin A and CGRP antagonists prevent sensory neuron-mediated exacerbation of psoriatic inflammation to similar extents as Asic3 KO. In contrast, replenishing CGRP in the skin of Asic3 KO mice restores the inflammatory response. These findings establish sensory ASIC3 as a critical constituent in psoriatic inflammation, and a promising target for neurogenic inflammation management.


Asunto(s)
Canales Iónicos Sensibles al Ácido , Péptido Relacionado con Gen de Calcitonina , Ratones Noqueados , Psoriasis , Células Receptoras Sensoriales , Animales , Canales Iónicos Sensibles al Ácido/metabolismo , Canales Iónicos Sensibles al Ácido/genética , Femenino , Psoriasis/metabolismo , Psoriasis/patología , Psoriasis/genética , Psoriasis/inducido químicamente , Ratones , Péptido Relacionado con Gen de Calcitonina/metabolismo , Péptido Relacionado con Gen de Calcitonina/genética , Células Receptoras Sensoriales/metabolismo , Piel/metabolismo , Piel/patología , Imiquimod , Ratones Endogámicos C57BL , Modelos Animales de Enfermedad , Inflamación/metabolismo , Inflamación Neurogénica/metabolismo , Humanos , Nociceptores/metabolismo , Interleucina-23/metabolismo , Interleucina-23/genética
15.
Am J Bot ; 100(9): 1860-70, 2013 Sep.
Artículo en Inglés | MEDLINE | ID: mdl-24018854

RESUMEN

PREMISE OF THE STUDY: Food crops of tropical origins, such as rice, are often cultivated in areas with suboptimal temperature regimes. The rice phytochrome B-deficient mutant (phyB) is tolerant of chilling temperatures compared with the wild type (WT) under low irradiance, and unsaturated fatty acids (USFAs) of membrane lipids have been shown to play an important role in chilling resistance. However, the relationship between phytochrome B and membrane lipids has not been empirically investigated. • METHODS: We assessed various photosynthesis indexes in phyB and WT rice: chlorophyll content, maximal photochemical efficiency (Fv/Fm) of photosystem II (PSII), the quantum yield of PSII electron transport (ΦPSII), the percentage of oxidizable P700 (P700), nonphotochemical quenching (NPQ), and the de-epoxidized ratio of xanthophyll cycle (A+Z)/(V+A+Z). We also analyzed the ultrastructure and fatty acid desaturases (FADs) and glycerol-3-phosphate acyltransferase (GPAT) genes of the chloroplasts using transmission electron microscopy and real-time PCR. • RESULTS: After a chilling treatment of 24 h, chloroplast damage and USFA content reduction were more severe in the WT than in the phyB mutant. Genes involved in the synthesis of USFAs in membranes such as FADs and GPAT were more stable in phyB than in WT. Chlorophyll content, Fv/Fm, ΦPSII, and P700 decreased more slowly under chilling stress and recovered more rapidly under optimal conditions in phyB than in WT. The (A+Z)/(V+A+Z) and NPQ increased more rapidly in phyB than in the WT after 24 h of chilling treatment. • CONCLUSIONS: Phytochrome B deficiency in rice with more stabilized chloroplast structure and higher USFA content in membrane lipids could alleviate chilling-induced photoinhibition.


Asunto(s)
Cloroplastos/ultraestructura , Oryza/fisiología , Fotosíntesis/fisiología , Fitocromo B/genética , Clorofila/metabolismo , Cloroplastos/metabolismo , Frío , Transporte de Electrón , Ácidos Grasos/análisis , Luz , Microscopía Electrónica de Transmisión , Modelos Biológicos , Mutación , Oryza/genética , Oryza/efectos de la radiación , Oryza/ultraestructura , Fenotipo , Complejo de Proteína del Fotosistema I/fisiología , Complejo de Proteína del Fotosistema II/fisiología , Fitocromo B/metabolismo , Hojas de la Planta/genética , Hojas de la Planta/fisiología , Hojas de la Planta/efectos de la radiación , Hojas de la Planta/ultraestructura , Plantas Modificadas Genéticamente , ARN Mensajero/genética , ARN de Planta/genética , Xantófilas/metabolismo
16.
PeerJ ; 11: e14647, 2023.
Artículo en Inglés | MEDLINE | ID: mdl-36643630

RESUMEN

Bactrian camels have specific mucosa-associated lymphoid tissue (MALT) throughout the large intestine, with species-unique cystic Peyer's patches (PPS) as the main type of tissue. However, detailed information about the molecular characteristics of PPS remains unclear. This study applied a transcriptomic analysis, untargeted metabolomics, and 16S rDNA sequencing to compare the significant differences between PPS and the adjacent normal intestine tissues (NPPS) during the healthy stage of three young Bactrian camels. The results showed that samples from PPS could be easily differentiated from the NPPS samples based on gene expression profile, metabolites, and microbial composition, separately indicated using dimension reduction methods. A total of 7,568 up-regulated and 1,266 down-regulated differentially expressed genes (DEGs) were detected, and an enrichment analysis found 994 DEGs that participated in immune-related functions, and a co-occurance network analysis identified nine hub genes (BTK, P2RX7, Pax5, DSG1, PTPN2, DOCK11, TBX21, IL10, and HLA-DOB) during multiple immunologic processes. Further, PPS and NPPS both had a similar pattern of most compounds among all profiles of metabolites, and only 113 differentially expressed metabolites (DEMs) were identified, with 101 of these being down-regulated. Deoxycholic acid (DCA; VIP = 37.96, log2FC = -2.97, P = 0), cholic acid (CA; VIP = 13.10, log2FC = -2.10, P = 0.01), and lithocholic acid (LCA; VIP = 12.94, log2FC = -1.63, P = 0.01) were the highest contributors to the significant dissimilarities between groups. PPS had significantly lower species richness (Chao1), while Firmicutes (35.92% ± 19.39%), Bacteroidetes (31.73% ± 6.24%), and Proteobacteria (13.96% ± 16.21%) were the main phyla across all samples. The LEfSe analysis showed that Lysinibacillus, Rikenellaceae_RC9_gut_group, Candidatus_Stoquefichus, Mailhella, Alistipes, and Ruminococcaceae_UCG_005 were biomarkers of the NPPS group, while Escherichia_Shigella, Synergistes, Pyramidobacter, Odoribacter, Methanobrevibacter, Cloacibacillus, Fusobacterium, and Parabacteroides were significantly higher in the PPS group. In the Procrustes analysis, the transcriptome changes between groups showed no significant correlations with metabolites or microbial communities, whereas the alteration of metabolites significantly correlated with the alteration of the microbial community. In the co-occurrence network, seven DEMs (M403T65-neg, M329T119-neg, M309T38-neg, M277T42-2-neg, M473T27-neg, M747T38-1-pos, and M482t187-pos) and 14 genera (e.g., Akkermansia, Candidatus-Stoquefichus, Caproiciproducens, and Erysipelatoclostridium) clustered much more tightly, suggesting dense interactions. The results of this study provide new insights into the understanding of the immune microenvironment of the cystic PPS in the cecum of Bactrian camels.


Asunto(s)
Camelus , Ganglios Linfáticos Agregados , Animales , Bacterias , Camelus/inmunología , Camelus/microbiología , Ciego/inmunología , Intestino Grueso/inmunología , Ganglios Linfáticos Agregados/inmunología , Multiómica
17.
Open Med (Wars) ; 18(1): 20230665, 2023.
Artículo en Inglés | MEDLINE | ID: mdl-36910848

RESUMEN

Laryngeal squamous cell carcinoma (LSCC) is an aggressive and lethal malignant neoplasm with extremely poor prognoses. Accumulating evidence has indicated that preferentially expressed antigen in melanoma (PRAME) is correlated with several kinds of cancers. However, there is little direct evidence to substantiate the biological function of PRAME in LSCC. The purpose of the current study is to explore the oncogenic role of PRAME in LSCC. PRAME expression was analyzed in 57 pairs of LSCC tumor tissue samples through quantitative real-time PCR, and the correlation between PRAME and clinicopathological features was analyzed. The result indicated that PRAME was overexpressed in the LSCC patients and correlated with the TNM staging and lymphatic metastasis. The biological functions and molecular mechanism of PRAME in LSCC progression were investigated through in vitro and in vivo assays. Functional studies confirmed that PRAME facilitated the proliferation, invasion, migration, and epithelial-mesenchymal transition of LSCC cells, and PRAME also promoted tumor growth in vivo. HDAC5 was identified as an upstream regulator that can affect the expression of PRAME. Moreover, PRAME played the role at least partially by activating PI3K/AKT/mTOR pathways. The above findings elucidate that PRAME may be a valuable oncogene target, contributing to the diagnosis and therapy of LSCC.

18.
Huan Jing Ke Xue ; 44(10): 5567-5577, 2023 Oct 08.
Artículo en Zh | MEDLINE | ID: mdl-37827773

RESUMEN

There are rich and diverse fungal communities in rainfall-cellar sediments. Fungi play a key role in the rainfall-cellar ecosystem as a bridge and link for material exchange between the rainfall-cellar ecosystem and the sediments. The changes in fungal community structure are usually closely related to the changes in environmental factors. The 16S rRNA gene Illumina MiSeq high-throughput sequencing technology was used to study the diversity and difference of fungal communities in the cellar sediments under two different catchment environments. The results revealed that the cellar sediments under the concrete catchment environment had higher diversity and richness of fungal communities than those under the loess land catchment environment. The dominant bacteria of the fungal communities under the two catchment environments were the same, namely Ascomycota, Basidiomycota, and Zygomycota, which constituted more than 90% of the abundance of the bacteria; however, the former had better homogeneity and stability. The indicator species based on LEfSe analysis demonstrated that Basidiobolales had the largest contribution to the diversity in the catchment environment of the loess land, and Mycosphaerella had the smallest contribution; Saccharomycetales contributed the most to the diversity in the concrete concentration environment, whereas Periconia contributed the least. The results of the co-occurrence network of the microbial community and environmental factors demonstrated that the positive relationship between fungi and environmental factors was stronger than the negative relationship. The research results have enhanced the understanding of the diversity of fungal communities in the cellar sediments and provided a reference for ensuring the drinking safety of rainwater harvesting cellar water for humans and livestock and improving the quality of cellar water.


Asunto(s)
Microbiota , Micobioma , Humanos , ARN Ribosómico 16S/genética , Hongos/genética , Agua
19.
Artículo en Inglés | MEDLINE | ID: mdl-37818576

RESUMEN

BACKGROUND: Yujin powder (YJP) is a classic prescription for treating dampness-heat diarrhea (DHD) in Traditional Chinese Medicine (TCM), but the main functional active ingredients and the exact mechanisms have not been systematically studied. OBJECTIVES: This study aimed to preliminarily explore the potential mechanisms of YJP for treating DHD by integrating UPLC-MS/MS and network pharmacology methods. METHODS: Ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) technology was used to determine the ingredients of YJP. And then, the targets of these components were predicted and screened from TCMSP, SwissTargetPrediction databases. The disease targets related to DHD were obtained by using the databases of GeneCards, OMIM, DisGeNET, TTD, and DrugBank. The protein-protein interaction networks (PPI) of YJP-DHD were constructed using the STRING database and Origin 2022 software to identify the cross-targets by screening the core-acting targets and a network diagram by Cytoscape 3.8.2 software was also constructed. Metascape database was used for performing GO and KEGG enrichment anlysis on the core genes. Finally, molecular docking was used to verify the results with AutoDock 4.2.6, AutoDock Tools 1.5.6, PyMOL 2.4.0, and Open Babel 2.3.2 software. RESULTS: 597 components in YJP were detected, and 153 active components were obtained through database screening, among them the key active ingredients include coptisine, berberine, baicalein, etc. There were 362 targets treating DHD, among them the core targets included TNF, IL-6, ALB, etc. The enriched KEGG pathways mainly involve PI3K-Akt, TNF, MAPK, etc. Molecular docking results showed that coptisine, berberine, baicalein, etc., had a strong affinity with TNF, IL-6, and MAPK14. Therefore, TNF, IL-6, MAPK14, ALB, etc., are the key targets of the active ingredients of YJP coptisine, baicalein, and berberine, etc. They have the potential to regulate PI3K-Akt, MAPK, and TNF signalling pathways. The component-target-disease network diagram revealed that YJP treated DHD through the effects of anti-inflammation, anti-diarrhea, immunoregulation, and improving intestinal mucosal injury. CONCLUSION: It is demonstrated that YJP treats DHD mainly through the main active ingredients coptisine, berberine, baicalein, etc. comprehensively exerting the effects of anti-inflammation, anti-diarrhea, immunoregulation, and improving intestinal mucosal injury, which will provide evidence for further in-depth studying the mechanism of YJP treating DHD.

20.
Appl Opt ; 51(10): C27-31, 2012 Apr 01.
Artículo en Inglés | MEDLINE | ID: mdl-22505107

RESUMEN

We demonstrated a high average power, narrow-linewidth, quasi-CW diode-pumped Nd:YAG 1064 nm laser with near-diffraction-limited beam quality. A symmetrical three-mirror ring cavity with unidirectional operation elements and an etalon was employed to realize the narrow-linewidth laser output. Two highly efficient laser modules and a 90° quartz rotator for birefringence compensation were used for the high output power. The maximum average output power of 62.5 W with the beam quality factor M(2) of 1.15 was achieved under a pump power of 216 W at a repetition rate of 500 Hz, corresponding to the optical-to-optical conversion efficiency of 28.9%. The linewidth of the laser at the maximum output power was measured to be less than 0.2 GHz.

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