Achieving Stable Lithium Metal Anode at 50 mA cm-2 Current Density by LiCl Enriched SEI.

Lithium metal batteries are intensively studied due to the potential to bring up breakthroughs in high energy density devices. However, the inevitable growth of dendrites will cause the rapid failure of battery especially under high current density. Herein, the utilization of tetrachloroethylene (C2 Cl4 ) is reported as the electrolyte additive to induce the formation of the LiCl-rich solid electrolyte interphase (SEI). Because of the lower Li ion diffusion barrier of LiCl, such SEI layer can supply sufficient pathway for rapid Li ion transport, alleviate the concentration polarization at the interface and inhibit the growth of Li dendrites. Meanwhile, the C2 Cl4 can be continuously replenished during the cycle to ensure the stability of the SEI layer. With the aid of C2 Cl4 -based electrolyte, the Li metal electrodes can maintain stable for >300 h under high current density of 50 mA cm-2 with areal capacity of 5 mAh cm-2 , broadening the compatibility of lithium metal anode toward practical application scenarios.

Ion-Inserted Metal-Organic Frameworks Accelerate the Mass Transfer Kinetics in Lithium-Sulfur Batteries.

Lithium-sulfur battery promises great potential to promote the reform of energy storage field. Modified functional interlayer on separator has been recognized as efficient method to promote battery performances, mainly focusing on the entrapment and catalytic effect toward lithium polysulfide, while the mass transfer property across the interlayers has not been carefully considered. Herein, a dense layer composed of ion-inserted metal-organic frameworks is used to facilitate mass transfer across the layer and ensure high polysulfides entrapment efficiency. In situ Raman study reveals that the dense functional layer blocks the transfer of Li ions, while the ion-inserted layer can accelerate the ion-transfer kinetics and avoid the ion depletion caused polarization. As a result, a specific capacity of 742 mAh g-1 is obtained at 2 C, with the decay rate of 0.089% per cycle at 1 C over 600 cycles, demonstrating great potential for the application in advanced Li-S batteries.

Coupling enhancement mechanisms, materials, and strategies for surface-enhanced Raman scattering devices.

Surface-enhanced Raman scattering (SERS) has become one of the most sensitive analytical techniques for identifying the chemical components, molecular structures, molecular conformations, and the interactions between molecules. However, great challenges still need to be addressed until it can be widely accepted by the absolute quantification of analytes. Recently, many efforts have been devoted to addressing these issues via various electromagnetic (EM), chemical (CM), and EM-CM hybrid coupling enhancement strategies. In comparison with uncoupled SERS devices, they offer key advantages in terms of sensitivity, reproducibility, uniformity, stability, controllability and reliability. This review provides an in-depth analysis of coupled SERS devices, including coupling enhancement mechanisms, materials and approaches. Finally, we also discuss the remaining bottlenecks and possible strategies for the development of coupling-enhanced SERS devices in the future.

Interfacial Capillary-Force-Driven Self-Assembly of Monolayer Colloidal Crystals for Supersensitive Plasmonic Sensors.

Colloidal lithography technology based on monolayer colloidal crystals (MCCs) is considered as an outstanding candidate for fabricating large-area patterned functional nanostructures and devices. Although many efforts have been devoted to achieve various novel applicatons, the quality of MCCs, a key factor for the controllability and reproducibility of the patterned nanostructures, is often overlooked. In this work, an interfacial capillary-force-driven self-assembly strategy (ICFDS) is designed to realize a high-quality and highly-ordered hexagonal monolayer MCCs array by resorting the capillary effect of the interfacial water film at substrate surface as well as controlling the zeta potential of the polystyrene particles. Compared with the conventional self-assembly method, this approach can realize the reself-assembly process on the substrate surface with few colloidal aggregates, vacancy, and crystal boundary defects. Furthermore, various typical large-scale nanostructure arrays are achieved by combining reactive ion etching, metal-assisted chemical etching, and so forth. Specifically, benefiting from the as-fabricated high-quality 2D hexagonal colloidal crystals, the surface plasmon resonance (SPR) sensors achieve an excellent refractive index sensitivity value of 3497 nm RIU-1 , which is competent for detecting bovine serum albumin with an ultralow concentration of 10-8 m. This work opens a window to prepare high-quality MCCs for more potential applications.

In situ evolved NiMo/NiMoO4 nanorods as a bifunctional catalyst for overall water splitting.

Due to their good conductivity and catalytic performance, Ni-Mo-based catalysts are well-established for highly effective water splitting. However, the know-how required to fabricate distinct interfaces and electronic structures for metal oxides is still a challenge, and the synergistic effect between metal and metal oxides that enhances electrocatalytic activity is still ambiguous. As described here, by controlling the lithium-induced conversion reaction of metal oxides, metal/metal-oxide composites with plentiful interfaces and prominent electrical interconnections were fabricated, which can boost active sites and accelerate mass transfer during electrocatalytic reactions. As a consequence, the superior catalytic activity of ECT-NiMo/NiMoO4 exhibited a low overpotential of 61 mV at a current density of 10 mA cm-2 for the hydrogen evolution reaction and 331 mV at 100 mA cm-2 for the oxygen evolution reaction. When integrated into a two-electrode system, the ECT-NiMo/NiMoO4 revealed a highly stable and efficient performance in overall water splitting. This work provides a promising approach to enhance the metallicity and electron redistribution of catalysts for numerous water-splitting applications and many other possibilities for energy storage devices.

Difference of genomic copy numbers alterations between hairy cell leukemia-variant and classical hairy cell leukemia: a pilot retrospective study in Chinese.

Background: Hairy cell leukemia (HCL) is a rare chronic B-cell lymphoproliferative disorder. It has two pathological subtypes: classical HCL (HCL-C) and HCL-variant (HCL-V). HCL-C and HCL-V are distinct in morphology and immunophenotype. Their differentiation is important for patient management and clinical outcome, with HCL-V responding poorly to conventional HCL treatments. Recently, whole genomic sequencing has been used to identify the difference between HCL-C and HCL-V and mutation of BRAFV600E has been proved to be a molecular hallmark of HCL-C. However, BRAF inhibitors were not effective in all HCL-C cases and HCL-V seems be lack of the high-frequency mutations. Therefore, it is necessary to compare the genomic changes between HCL-C and HCL-V by high-resolution studies, especially in Chinese population, the genomic alterations of HCL have rarely be reported. Methods: In this study, the clinical features of a total of 18 Chinese HCL patients were described. Single nucleotide polymorphism (SNP) array analysis was performed to evaluate the genomic copy number alterations (CNA) and copy neutral loss of heterozygosity (CN-LOH) on six HCL-Vs with CD25-/BRAFV600E- and four HCL-Cs with CD25+/BRAFV600E+. Results: A total of 24 CNAs including seven chromosomal gains and 17 chromosomal losses, and 22 CN-LOHs were revealed. Five of the six cases of HCL-V showed 15 CNAs including four cryptic chromosomal gains and 11 chromosomal losses. Overlapping regions involving micro-deletion of chromosome 2q13 and large chromosomal loss of 14q were showed in HCL-V. In HCL-C, a total of nine CNAs were revealed in three of the four cases including three chromosomal gains and six chromosomal losses. No overlapping area was observed among the CNVs. 15 CN-LOHs were showed in five of the six cases of HCL-V and seven CN-LOHs was demonstrated in all of the four HCL-Cs. Conclusions: Comparing to Westerners, a relatively higher proportion of HCL-V in all HCL is observed in this study. CNAs and CN-LOHs were common in both HCL-V and HCL-C but the CNAs were different in them. HCL-C was characterized with the higher ratio of large chromosomal changes but lacked of recurrent CNAs, while HCL-V was presented with the higher incidence of cryptic CNAs and recurrent CNAs involving tumor-associated genes. It is necessary to further investigate the association of the genes, such as NPHP1 and TRAF3 genes, and HCL-V in the future study.

Infantile Epithelioid Sarcoma with Genomic Segmental Amplification of BIRC3/YAP1 as Double Minutes Plus Trisomy 2: A Case Report.

Introduction: Epithelioid sarcoma is a malignant mesenchymal tumor exhibiting epithelioid cytomorphology and epithelial phenotype. Its histogenesis is unknown, but its tumorigenesis may relate to inactivation of hSNF5/SMARCB1/INI1 tumor suppressor gene. This tumor typically affects young adults and older children, but it is uncommon in infants. Case Report: We describe a unique neoplasm in a 15-month-old infant presenting with a heel mass. The tumor was remarkable for retention of SMARCB1/INI1 expression. Conventional cytogenetic analysis revealed trisomy 2 and double minutes, and SNP array analysis confirmed the trisomy 2 and identified segmental amplification of chromosome 11 containing YAP1 and BIRC3; FISH testing proved that the double minutes consisted of BIRC3 and YAP1, potent oncogenes related to tumorigenesis of several types of tumors but not described in epithelioid sarcoma. Conclusion: Our findings expand the spectrum of cytogenetic alterations in this neoplasm, help in better understanding its tumorigenesis, and suggest potential therapeutic targets.

Aluminum-Tailored Energy Level and Morphology of Co3- x Alx O4 Porous Nanosheets toward Highly Efficient Electrocatalysts for Water Oxidation.

Tuning energy levels plays a crucial role in developing cost-effective, earth-abundant, and highly active oxygen evolution catalysts. However, to date, little attention has been paid to the effect of using heteroatom-occupied lattice sites on the energy level to engineer electrocatalytic activity. In order to explore heteroatom-engineered energy levels of spinel Co3 O4 for highly-effective oxygen electrocatalysts, herein Al atoms are directly introduced into the crystal lattice by occupying the Co2+ ions in the tetrahedral sites and Co3+ ions in the octahedral sites (denoted as Co2+ Td and Co3+ Oh , respectively). Experimental and theoretical simulations demonstrate that Al3+ ions substituting Co2+ Td and Co3+ Oh active sites, especially Al3+ ions occupying the Co2+ Td sites, optimizes the adsorption, activation, and desorption features of intermediate species during oxygen evolution reaction (OER) processes. As a result, the optimized Co1.75 Al1.25 O4 nanosheet exhibit unprecedented OER activity with an ultralow overpotential of 248 mV to deliver a current of 10 mA cm-2 , among the best Co-based OER electrocatalysts. This work should not only provide fundamental understanding of the effect of Al-occupied different Co sites in Co3-x Alx O4 composites on OER performance, but also inspire the design of low-cost, earth-abundant, and high-active electrocatalysts toward water oxidation.

Cytogenetic characterization of the malignant primitive neuroectodermal SK-PN-DW tumor cell line.

BACKGROUND: The SK-PN-DW cell line was established in 1979 and is commercially available. Despite the use of this cell line as an in vitro model for functional and therapeutic studies of malignant primitive neuroectodermal tumor (PNET), there is a lack of complete information about the genetic alterations that are present at the cytogenetic level. Thus, the current study aimed to characterize the cytogenetic profile of this cell line. METHODS: Routine G-banded chromosome analysis, fluorescence in situ hybridization, and oligonucleotide array comparative genomic hybridization assays were performed to characterize the chromosomal changes in this cell line. RESULTS: The G-banded karyotype analysis showed that the number of chromosomes in this cell line ranged between 36 and 41. Importantly, all cells displayed a loss of chromosomes Y, 11, 13, and 18. However, some cells showed an additional loss of chromosome 10. Additionally, the observed structural changes indicated: a) unbalanced translocation between chromosomes 1 and 7; b) translocation between chromosomes 11 and 22 at breakpoints 11q24 and 22q12, which is a classical translocation that is associated with Ewing sarcoma; c) a derivative chromosome due to a whole arm translocation between chromosomes 16 and 17 at likely breakpoints 16p10 and 17q10; and d) possible rearrangement in the short arm of chromosome 18. Moreover, a variable number of double minutes were also observed in each metaphase cell. Furthermore, the microarray assay results not only demonstrated genomic-wide chromosomal imbalance in this cell line and precisely placed chromosomal breakpoints on unbalanced, rearranged chromosomes, but also revealed information about subtle chromosomal changes and the chromosomal origin of double minutes. Finally, the fluorescence in situ hybridization assay confirmed the findings of the routine cytogenetic analysis and microarrays. CONCLUSION: The accurate determination of the cytogenetic profile of the SK-PN-DW cell line is helpful in enabling the research community to utilize this cell line for future identity and comparability studies, in addition to demonstrating the utility of the complete cytogenetic profile, as a public resource.

Identification of Key Reversible Intermediates in Self-Reconstructed Nickel-Based Hybrid Electrocatalysts for Oxygen Evolution.

The oxygen evolution reaction (OER) has been explored extensively for reliable hydrogen supply to boost the energy conversion efficiency. The superior OER performance of newly developed non-noble metal electrocatalysts has concealed the identification of the real active species of the catalysts. Now, the critical active phase in nickel-based materials (represented by NiNPS) was directly identified by observing the dynamic surface reconstruction during the harsh OER process via combining in situ Raman tracking and ex situ microscopy and spectroscopy analyses. The irreversible phase transformation from NiNPS to α-Ni(OH)2 and reversible phase transition between α-Ni(OH)2 and γ-NiOOH prior to OER demonstrate γ-NiOOH as the key active species for OER. The hybrid catalyst exhibits 48-fold enhanced catalytic current at 300 mV and remarkably reduced Tafel slope to 46 mV dec-1 , indicating the greatly accelerated catalytic kinetics after surface evolution.

Single-Nanostructured Electrochemical Detection for Intrinsic Mechanism of Energy Storage: Progress and Prospect.

Energy storage appliances are active by means of accompanying components for renewable energy resources that play a significant role in the advanced world. To further improve the electrochemical properties of the lithium-ion batteries (LIBs), sodium-ion batteries (SIBs), and lithium-sulfur (Li-S) batteries, the electrochemical detection of the intrinsic mechanisms and dynamics of electrodes in batteries is required to guide the rational design of electrodes. Thus, several researches have conducted in situ investigations and real-time observations of electrode evolution, ion diffusion pathways, and side reactions during battery operation at the nanoscale, which are proven to be extremely insightful. However, the in situ cells are required to be compatible for electrochemical tests and are therefore often challenging to operate. In the past few years, tremendous progresses have been made with novel and more advanced in situ electrochemical detection methods for mechanism studies, especially single-nanostructured electrodes. Herein, a comprehensive review of in situ techniques based on single-nanostructured electrodes for studying electrodes changes in LIBs, SIBs, and Li-S batteries, including structure evolution, phase transition, interface formation, and the ion diffusion pathway is provided, which is instructive and meaningful for the optimization of battery systems.

Redox Chemistry of Molybdenum Trioxide for Ultrafast Hydrogen-Ion Storage.

Hydrogen ions are ideal charge carriers for rechargeable batteries due to their small ionic radius and wide availability. However, little attention has been paid to hydrogen-ion storage devices because they generally deliver relatively low Coulombic efficiency as a result of the hydrogen evolution reaction that occurs in an aqueous electrolyte. Herein, we successfully demonstrate that hydrogen ions can be electrochemically stored in an inorganic molybdenum trioxide (MoO3 ) electrode with high Coulombic efficiency and stability. The as-obtained electrode exhibits ultrafast hydrogen-ion storage properties with a specific capacity of 88 mA hg-1 at an ultrahigh rate of 100 C. The redox reaction mechanism of the MoO3 electrode in the hydrogen-ion cell was investigated in detail. The results reveal a conversion reaction of the MoO3 electrode into H0.88 MoO3 during the first hydrogen-ion insertion process and reversible intercalation/deintercalation of hydrogen ions between H0.88 MoO3 and H0.12 MoO3 during the following cycles. This study reveals new opportunities for the development of high-power energy storage devices with lightweight elements.

TiO2 Feather Duster as Effective Polysulfides Restrictor for Enhanced Electrochemical Kinetics in Lithium-Sulfur Batteries.

The rechargeable lithium-sulfur battery is recognized as a promising candidate for electrochemical energy storage system because of their exceptional advance in energy density. However, the fast capacity decay of sulfur cathode caused by polysulfide dissolution and low specific capacity caused by poor electrical conductivity still impede the further development of lithium-sulfur battery. To address above issues, this study reports the synthesis of feather duster-like TiO2 architecture by in situ growth of TiO2 nanowires on carbon cloth and further evaluates as sulfur host material. The strong chemical binding interaction between the polysulfides and TiO2 feather duster efficiently restrains the shuttle effect, leading to enhanced electrochemical kinetics. Besides, the in situ grown TiO2 NWs array also supply high surface for sulfur-loading and fast path for electron transfer and ion diffusion. As results, the novel CC/TiO2 /S composite cathode exhibits a high capacity of 608 mA h g-1 at 1.0 C after 700 cycles corresponding to capacity decay as low as 0.045% per cycle with excellent Coulombic efficiency higher than 99.5%.

New H6 influenza virus reassortment strains isolated from Anser fabalis in Anhui Province, China.

BACKGROUND: H6 subtype avian influenza viruses are globally distributed and, in recent years, have been isolated with increasing frequency from both domestic and wild bird species as well as infected humans. Many reports have examined the viruses in the context of poultry or several wild bird species, but there is less information regarding their presence in migratory birds. METHODS: Hemagglutination and hemagglutination inhibition tests were used to measure HA activity for different HA subtypes. Whole viral genomes were sequenced and analysed using DNAstar and MEGA 6 to understand their genetic evolution. Pathogenicity was evaluated using a mouse infection model. RESULTS: We isolated 13 strains of H6 virus from faecal samples of migratory waterfowl in Anhui Province of China in 2014. Phylogenetic analysis showed gene reassortment between Eurasian and North American lineages. Five of the identified H6 strains had the ability to infect mice without adaptation. CONCLUSION: Our findings suggest that regular surveillance of wild birds, especially migratory birds, is important for providing early warning and control of avian influenza outbreaks.

Engineered nanomembranes for smart energy storage devices.

Engineered nanomembranes are of great interest not only for large-scale energy storage devices, but also for on-chip energy storage integrated microdevices (such as microbatteries, microsupercapacitors, on-chip capacitors, etc.) because of their large active surfaces for electrochemical reactions, shortened paths for fast ion diffusion, and easy engineering for microdevice applications. In addition, engineered nanomembranes provide a lab-on-chip electrochemical device platform for probing the correlations of electrode structure, electrical/ionic conductivity, and electrochemical kinetics with device performance. This review focuses on the recent progress in engineered nanomembranes including tubular nanomembranes and planar nanomembranes, with the aim to provide a systematic summary of their fabrication, modification, and energy storage applications in lithium-ion batteries, lithium-oxygen batteries, on-chip electrostatic capacitors and micro-supercapacitors. A comprehensive understanding of the relationship between engineered nanomembranes and electrochemical properties of lithium ion storage with engineered single-tube microbatteries is given, and the flexibility and transparency of micro-supercapacitors is also discussed. Remarks on challenges and perspectives related to engineered nanomembranes for the further development of energy storage applications conclude this review.

Amplification and overexpression of CTTN and CCND1 at chromosome 11q13 in Esophagus squamous cell carcinoma (ESCC) of North Eastern Chinese Population.

Esophageal squamous cell carcinoma (ESCC) is a genetically complex tumor type and is a major cause of cancer-related mortality. The combination of genetics, diet, behavior, and environment plays an important role in the carcinogenesis of ESCC. To characterize the genomic aberrations of this disease, we investigated the genomic imbalances in 19 primary ESCC cases using high-resolution array comparative genomic hybridization (CGH). All cases showed either loss or gain of whole chromosomes or segments of chromosome(s) with variable genomic sizes. The copy number alterations per case affected the median 34% (~ 1,034Mb/3,000Mb) of the whole genome. Recurrent gains were 1q21.3-qter, 3q13.11-qter, 5pter-p11, 7pter-p15.3, 7p12.1-p11.2, 7q11-q11.2, 8p12-qter, 11q13.2-q13.3, 12pter-p13.31, 17q24.2, 20q11.21-qter, and 22q11.21-q11.22 whereas the recurrent losses were 3pter-p11.1, 4pter-p12, 4q28.3-q31.22, 4q31.3-q32.1, 9pter-p12, 11q22.3-qter and 13q12.11-q22.1. Amplification of 11q13 resulting in overexpression of CTTN/CCND1 was the most prominent finding, which was observed in 13 of 19 ESCC cases. These unique profiles of copy number alteration should be validated by further studies and need to be taken into consideration when developing biomarkers for early detection of ESCC.

Genomic Copy Number Variations in the Myelodysplastic Syndrome and Acute Myeloid Leukemia Patients with del(5q) and/or -7/del(7q).

The most common chromosomal abnormalities in myelodysplastic syndrome (MDS) and acute myeloid leukemia (AML) are -5/del(5q) and -7/del(7q). When -5/del(5q) and -7/del(7q) coexist in patients, a poor prognosis is typically associated. Given that -5/del(5q) and/or -7/del(7q) often are accompanied with additional recurrent chromosomal alterations, genetic change(s) on the accompanying chromosome(s) other than chromosomes 5 and 7 may be important factor(s) affecting leukemogenesis and disease prognosis. Using an integrated analysis of karyotype, FISH and array CGH results in this study, we evaluated the smallest region of overlap (SRO) of chromosomes 5 and 7 as well as copy number alterations (CNAs) on the other chromosomes. Moreover, the relationship between the CNAs and del(5q) and -7/del(7q) was investigated by categorizing the cases into three groups based on the abnormalities of chromosomes 5 and 7 [group I: cases only with del(5q), group II: cases only with -7/del(7q) and group III: concurrent del(5q) and del(7q) cases]. The overlapping SRO of chromosome 5 from groups I and III was 5q31.1-33.1 and of chromosome 7 from groups II and III was 7q31.31-q36.1. A total of 318 CNAs were observed; ~ 78.3% of them were identified on chromosomes other than chromosomes 5 and 7, which were defined as 'other CNAs'. Group III was a distinctive group carrying the most high number (HN) CNAs, cryptic CNAs and 'other CNAs'. The loss of TP53 was highly associated with del(5q). The loss of ETV6 was specifically associated with group III. These CNAs or genes may play a secondary role in disease progression and should be further evaluated for their clinical significance and influence on therapeutic approaches in patients with MDS/AML carrying del(5q) and/or -7/del(7q) in large-scale, patient population study.

Synthesis, Fluorescence Properties, and Antiproliferative Potential of Several 3-Oxo-3H-benzo[f]chromene-2-carboxylic Acid Derivatives.

In this study, two series of 3-oxo-3H-benzo[f]chromene-2-carboxylic acid derivatives (compounds 5a-i and 6a-g) were synthesized. Their in vitro proliferation inhibitory activities against the A549 and NCI-H460 human non-small cell lung cancer (NSCLC) cell lines were evaluated. Their photophysical properties were measured. Among these target compounds, 5e exhibited the strongest antiproliferative activity by inducing apoptosis, arresting cell cycle, and elevating intracellular reactive oxygen species (ROS) level, suggesting that it may be a potent antitumor agent. In addition, compound 6g with very low cytotoxicity, demonstrated excellent fluorescence properties, which could be used as an effective fluorescence probe for biological imaging.

Fiber-based flexible all-solid-state asymmetric supercapacitors for integrated photodetecting system.

Integrated nanodevices with the capability of storing energy are widely applicable and have thus been studied extensively. To meet the demand for flexible integrated devices, all-solid-state asymmetric supercapacitors that simultaneously realize energy storage and optoelectronic detection were fabricated by growing Co3 O4 nanowires on nickel fibers, thus giving the positive electrode, and employing graphene as both the negative electrode and light-sensitive material. The as-assembled integrated systems were characterized by an improved energy storage, enhanced power density (at least by 1860 % enhanced) by improving the potential window from 0-0.6 V to 0-1.5 V, excellent photoresponse to white light, and superior flexibility of both the fiber-based asymmetric supercapacitor and the photodetector. Such flexible integrated devices might be used in smart and self-powered sensory, wearable, and portable electronics.

Ultralong Cycling and Safe Lithium-Sulfur Pouch Cells for Sustainable Energy Storage.

While layered metal oxides remain the dominant cathode materials for the state-of-the-art lithium-ion batteries, conversion-type cathodes such as sulfur present unique opportunities in developing cheaper, safer, and more energy-dense next-generation battery technologies. There has been remarkable progress in advancing the laboratory scale lithium-sulfur (Li-S) coin cells to a high level of performance. However, the relevant strategies cannot be readily translated to practical cell formats such as pouch cells and even battery pack. Here these key technical challenges are addressed by molecular engineering of the Li metal for hydrophobicization, fluorination and thus favorable anode chemistry. The introduced tris(2,4-di-tert-butylphenyl) phosphite (TBP) and tetrabutylammonium fluoride (TBA+F-) as well as cellulose membrane by rolling enables the formation of a functional thin layer that eliminates the vulnerability of Li metal towards the already demanding environment required (1.55% relative humidity) for cell production and gives rise to LiF-rich solid electrolyte interphase (SEI) to suppress dendrite growth. As a result, Li-S pouch cells assembled at a pilot production line survive 400 full charge/discharge cycles with an average Coulombic efficiency of 99.55% and impressive rate performance of 1.5 C. A cell-level energy density of 417 Wh kg-1 and power density of 2766 W kg-1 are also delivered via multilayer Li-S pouch cell. The Li-S battery pack can even power an unmanned aerial vehicle of 3 kg for a fairly long flight time. This work represents a big step forward acceleration in Li-S battery marketization for future energy storage featuring improved safety, sustainability, higher energy density as well as reduced cost.