Shedding light on cellular dynamics: the progress in developing photoactivated fluorophores.

Photoactivated fluorophores (PAFs) are highly effective imaging tools that exhibit a removal of caging groups upon light excitation, resulting in the restoration of their bright fluorescence. This unique property allows for precise control over the spatiotemporal aspects of small molecule substances, making them indispensable for studying protein labeling and small molecule signaling within live cells. In this comprehensive review, we explore the historical background of this field and emphasize recent advancements based on various reaction mechanisms. Additionally, we discuss the structures and applications of the PAFs. We firmly believe that the development of more novel PAFs will provide powerful tools to dynamically investigate cells and expand the applications of these techniques into new domains.

Fungal community composition changes and reduced bacterial diversity drive improvements in the soil quality index during arable land restoration.

Arable land is facing the growing challenge of land degradation due to intensive use and this is beginning to affect global food security. However, active and passive restoration can improve soil characteristics and reshape microbial communities. Despite the increasing focus on changes in microbial communities during restoration, the mechanisms underlying how microbes drive the soil quality index (SQI) in arable land restoration remain unclear. In this study, we selected conventional farmland (CF, heavily intensified) and two restoration strategies (AR, artificial restoration; NR, natural restoration), with the same context (including soil texture, climate, etc.), and measured the microbial indicators over 2 years to investigate the mechanisms driving SQI improvement on restored arable land. The AR and NR treatments resulted in a 50% and 58% increase in SQI, respectively, compared to CF as soil nutrient levels increased, resulting in higher microbial biomasses and enzyme activities. Microbial abundance on the AR land was approximately two times greater than on the NR land due to the introduction of legumes. Bacterial diversity declined, while fungi developed in a more diverse direction under the restoration strategies. The AR and NR areas were mainly enriched with rhizobium (Microvirga, Bradyrhizobium), which contribute to healthy plant growth. The pathogenic fungi (Gibberella, Fusarium, Volutella) were more abundant in the CF area and the plant pathogen guild was about five times higher in the restored areas. Following arable land restoration, microbial life history strategies shifted from r-to K-strategists due to the higher proportion of recalcitrant SOC (DOC/SOC decreased by 18%-30%). The altered microbial community in the restored areas created new levels of functionality, with a 2.6%-4.3% decrease in bacterial energy metabolism (oxidative phosphorylation, C fixation, and N metabolism decreased by 7%, 4%, and 6%, respectively). Structural equation modelling suggested that restoration strategy affected SQI either directly by increasing total soil nutrient levels or indirectly by altering the microbial community and that fungal community composition and bacterial diversity made the largest contributions to SQI. These results provided new insights into soil quality improvement from a microbial perspective and can help guide future arable land restoration.

Plastic film mulching application improves potato yields, reduces ammonia emissions, but boosts the greenhouse gas emissions in China.

With global population growth and climate change, food security and global warming have emerged as two major challenges to agricultural development. Plastic film mulching (PM) has long been used to improve yields in rain-fed agricultural systems, but few studies have focused on soil gas emissions from mulched rainfed potatoes on a long-term and regional scale. This study integrated field data with the Denitrification-Decomposition (DNDC) model to evaluate the impacts of PM on potato yields, greenhouse gas (GHG) and ammonia (NH3) emissions in rainfed agricultural systems in China. We found that PM increased potato yield by 39.7 % (1505 kg ha-1), carbon dioxide (CO2) emissions by 15.4 % (123 kg CO2 eq ha-1), nitrous oxide (N2O) emissions by 47.8 % (1016 kg CO2 eq ha-1), and global warming potential (GWP) by 38.9 % (1030 kg CO2 eq ha-1), while NH3 volatilization decreased by 33.9 % (8.4 kg NH3 ha-1), and methane (CH4) emissions were little changed compared to CK. Specifically, the yield after PM significantly increased in South China (SC), North China (NC), and Northwest China (NWC), with increases of 66.1 % (2429 kg ha-1), 44.1 % (1173 kg ha-1), and 43.6 % (956 kg ha-1) compared to CK, respectively. The increase in GWP and greenhouse gas emission intensity (GHGI) under PM was more pronounced in the Northeast China (NEC) and NWC regions, with respective increases of 57.1 % and 60.2 % in GWP, 16.9 % and 10.3 % in GHGI. While in the Middle and Lower reaches of the Yangtze River (MLYR) and SC, PM decreased GHGI with 10.2 % and 31.1 %, respectively. PM significantly reduced NH3 emissions in all regions and these reductions were most significant in Southwest China (SWC), SCand MLYR, which were 41 %, 38.0 %, and 38.0 % lower than CK, respectively. In addition, climatic and edaphic variables were the main contributors to GHG and NH3 emissions. In conclusion, it is appropriate to promote the use of PM in the MLYR and SC regions, because of the ability to increase yields while reducing environmental impacts (lower GHGI and NH3 emissions). The findings provide a theoretical basis for sustainable agricultural production of PM potatoes.

[Single-use Medical Devices Re-processing: Risk Assessment and Quality Control Technologies].

The reuse of high-cost single-use medical devices (SUD) is permitted in many countries, such as the United States, Germany and the United Kingdom, but strict regulatory requirements must be met. In addition to regulatory policies and regulations, such as market access mode and special requirements on Good Manufacture Practice (GMP), there are strict technical requirements on the potential risk control and quality assurance system. Therefore, effective risk assessment and risk control technology are the keys to ensure effective quality control and safe use of SUDs. In this article, based on analyzing the technological requirements of the national regulatory on SUDs in the United States, Germany and Britain, and combined with the review from latest relevant literature, to discuss the strategies of how to carry out scientific risk assessment. Some risk control technologies on the reuse of SUDs are introduced, which will provide support for the further study on risk control strategies and regulatory decisions for the reuse of SUDs in China.

[Single-use Medical Devices Re-processing: Regulatory Status Quo].

Some single-use medical devices are reprocessed and reused in some countries in the world, but the regulatory approach is different, and in some countries it isn't regulated yet. In this article, the regulatory status quo of single-use medical devices is reviewed. The regulatory development, important regulatory documents and regulatory approaches of single-use medical device reprocessing in the United States, Germany and the UK are introduced. And how to perform scientific risk assessment and effective risk control is discussed. The information is useful to establish China-specific regulations, and to develop relevant standards, guidelines or specifications and the risk control strategies.

Effects of sodium bicarbonate concentration on growth, photosynthesis, and carbonic anhydrase activity of macroalgae Gracilariopsis lemaneiformis, Gracilaria vermiculophylla, and Gracilaria chouae (Gracilariales, Rhodophyta).

There is potential for bicarbonate to improve crop yields and economic efficiency of marine algae. However, few studies have focused on the effect of bicarbonate on the growth, photosynthesis, and enzyme activity associated with carbon utilization, especially in commercial macroalgae. Here, the addition of bicarbonate (up to 420 mg L(-1)) to macroalgal cultures has been evaluated for Gracilariopsis lemaneiformis, Gracilaria vermiculophylla, and Gracilaria chouae with respect to growth rate, photosynthetic activity, carbonic anhydrase activity, and biochemical composition. The results showed that the effects of NaHCO3 on growth, chlorophyll a, phycoerythrin, photosynthetic oxygen evolution, photochemical parameters of PSI and PSII, carbonic anhydrase activity, and nitrogen content were significant (P < 0.05) and followed the same pattern in the three species. The parameter values were promoted in lower NaHCO3 concentrations (up to 252 or 336 mg L(-1)) and inhibited in higher NaHCO3 concentrations (>336 mg L(-1) for Gp. lemaneiformis and >420 mg L(-1) for the other two species). Moreover, species-specific differences induced by supplementation with bicarbonate were discovered during culture. Optimal concentrations of NaHCO3 used in this study were 252 mg L(-1) for Gp. lemaneiformis and 336 mg L(-1) for G. vermiculophylla and G. chouae. These results suggest that an adequate supplementation of sodium bicarbonate is a viable strategy for promoting growth and photosynthetic activity in some macroalgae as well as for improving biochemical composition. The study will help to accelerate the growth rate of algae and improve the quality of thalli, and will also be useful for enhancing the understanding of carbon utilization in macroalgae.

[Simultaneous determination of clevidipine butyrate and its metabolite clevidipine acid in dog blood by liquid chromatography-tandem mass spectrometry].

A rapid, sensitive and simple liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed and validated for the simultaneous determination of clevidipine butyrate and its primary metabolite clevidipine acid in dog blood. After one-step protein precipitation with methanol, the chromatographic separation was carried out on an Ecosil C18 column (150 mm x 4.6 mm, 5 µm) with a gradient mobile phase consisting of methanol and 5 mmol · L(-1) ammonium formate. A chromatographic total run time of 13.0 min was achieved. The quantitation analysis was performed using multiple reaction monitoring (MRM) at the specific ion transitions of m/z 454.1 [M-H]- --> m/z 234.1 for clevidipine butyrate, m/z 354.0 [M-H]- --> m/z 208.0 for clevidipine acid and m/z 256.1 [M-H]- --> m/z 227.1 for elofesalamide (internal standard, IS) in the negative ion mode with electrospray ionization (ESI) source. The linear calibration curves for clevidipine butyrate and clevidipine acid were obtained in the concentration ranges of 0.5-100 ng · mL and 1-200 ng · mL(-1), separately. The lower limit of quantification of clevidipine butyrate and clevidipine acid were 0.5 ng · mL(-1) and 1 ng · mL(-1). The intra and inter-assay precisions were all below 12.9%, the accuracies were all in standard ranges. Stability testing indicated that clevidipine butyrate and clevidipine acid in dog blood with the addition of denaturant methanol was stable under various processing and/or handling conditions. The validated method has been successfully applied to a pharmacokinetic study of clevidipine butyrate injection to 8 healthy Beagle dogs following intravenous infusion at a flow rate of 5 mg · h(-1) for 0.5 h.

Grazing alters the relationships between species diversity and biomass during community succession in a semiarid grassland.

The relationships between biodiversity and ecosystem functions (BEF) are crucial for ecosystem management. However, little is known about how grazing affects BEF relationships in the context of ecological succession. Here, using a 5-year experiment in a semiarid grassland of the Loess Plateau, China, we mainly focused on how grazing affected the relationships between plant species diversity and aboveground biomass (AGB) and explored the underlying mechanisms behind the relationships. In addition, we compared the plant dynamics of community composition and structure under no-grazing and grazing treatments during succession. We found that the plant species diversity-AGB relationship shifted from a negative-linear pattern in no-grazing to a humped-back model in grazing during plant community succession, suggesting that grazing could regulate dominant species and alter the availability of light resources to suppress competitive exclusion during succession. In addition, changes in annual plants over time played crucial roles in the BEF relationships. The increase in annual Salsola collina in this study, which also alters multiple mechanisms of plant interaction, had a significant effect on the negative-linear relationship both with and without grazing. On average, compared to no-grazing treatment, grazing significantly decreased the plant community density (39.53 %), cover (16.97 %), height (7.85 %), and AGB (9.35 %), but increased plant diversity, including species richness and the Shannon-Wiener index, and especially dramatically enhanced the Shannon-Wiener index (ranging from 1.55 to 2.13). These results underline the close association between grazing and the dynamics of plant communities in semiarid grasslands during succession. In particular, our findings further reveal grazing-dependent relationships between diversity and AGB, which have significant implications for the management and biodiversity conservation measures of semiarid grassland ecosystems.

Optimum plastic mulching application to reduce greenhouse gas emissions without compromising on crop yield and farmers' income.

With the rapid socio-economic development in China, poverty alleviation and the reduction of the environmental footprint in the plastic film mulching (PM) planting system have become key to sustainable agricultural production. Although many studies have evaluated the maize yield, agricultural economic benefits, and greenhouse gas (GHG) emissions associated with PM through small-scale field experiments, identifying suitable PM regions in combination with their demographic characteristics and the future development of such systems has received little attention. This study combines a Denitrification-Decomposition (DNDC) model and demographic characteristics to determine the optimum PM region in rainfed areas of the Loess Plateau in northwest China. The results demonstrated that PM produced a higher maize yield, agricultural net profit (ANP), and cost-benefit ratio compared to a control treatment (CK) without PM. An agricultural income far above the poverty level would assist in meeting the goals of alleviating poverty and building a prosperous society. In addition, the PM system produced more GHG emissions, but had a lower greenhouse gas intensity (GHGI) than CK under both low (200 kg N ha-1) and high (300 kg N ha-1) nitrogen (N) fertilizer rates. This study developed a framework to evaluate maize yield alongside economic and environmental indicators. We concluded that PM should be adopted in areas with precipitation less than 500 mm, and concentrated in the region with rainfall of 200-400 mm. The results provide a theoretical basis for the sustainable development of the PM maize planting system, and will contribute to the desired goal of environmentally sustainable agricultural production.

The effects of plastic film mulching and straw mulching on licorice root yield and soil organic carbon content in a dryland farming.

The increasing worldwide demand for traditional herbs has been met by growing cultivated herbs. It is undoubtedly very important to seek a reasonable cultivation mode for the yield, quality and long-term production stability of traditional herbs. In this study, licorice (Glycyrrhiza uralensis Fisch.) was investigated using a field experiment and a process-based model (Denitrification-Decomposition (DNDC) model) to study the effects of mulching methods on root yield and soil organic carbon (SOC) long-term changes. The field experiment contained four treatments: plat planting without mulching (CK), ridge-furrow maize straw mulching (SM), ridge-furrow plastic film mulching (RP), and plat planting with plastic film mulching (FP). Licorice root yield was significantly higher in the SM, RP, and FP than in the CK. SM, RP and FP treatments increased the accumulation of liquiritin and glycyrrhizin in licorice roots. The SM significantly increased SOC content, SOC stocks, SOC sequestration rate, dissolved organic carbon (DOC) content and microbial biomass carbon (MBC) compared to CK, but there was no significant difference in SOC and DOC among CK, RP and FP. The DNDC model was calibrated based on the field test data and showed that under the four CMIP6 SSPs scenarios, the predicted root yield of each treatment was increasing obviously. The production and stability of RP and FP were greater than CK and SM. The SOC under SM showed an increasing trend, whereas it continuously decreased under CK, RP, and FP in the future. The SOC of simulated RS treatment of straw incorporation plus a plastic film mulch was always at the highest value in all the treatments, and its root yield was slightly lower than that of RP and FP, the latter both were very close. Therefore, it is suggested that RS should be adopted to achieve sustained high yield while maintaining a high SOC level.

Conservation tillage or plastic film mulching? A comprehensive global meta-analysis based on maize yield and nitrogen use efficiency.

Rain-fed agriculture is an important part of the global agriculture system and plays a vital role in ensuring food security. Conservation tillage (CT) is widely used in USA maize cultivation in the Mid-west Corn Belt. Meanwhile, Chinese farmers' adoption of CT is limited and plastic film mulching (PM) is widely developed to increase maize yield in northern China. This paper compared the yield and nitrogen use efficiency (NUE) between CT and PM, and analyzed the reasons for the differences in the choice of maize cultivation practice. We collected 767 observations for CT and 217 observations for PM and analyzed how these two tillage practices affected yield. For NUE, there were 66 and 56 observations, respectively. The meta-analysis showed that PM significantly increased maize yield by 36% and NUE by 34% compared with the control, and CT significantly decreased maize yield by 5% and NUE by 15%. The effects of PM on maize yield were mainly determined by growing season precipitation (GSP) and temperature (GST). The yield response ratio was also influenced by the type of plastic film, mulching cycle, pH, soil saturated hydraulic conductivity, and soil bulk density. The negative effects of CT on yield could be alleviated under good hydrothermal conditions and with straw mulching. The yield response ratio was also affected by soil texture and N application rate. In conclusion, PM should be applied under limited hydrothermal conditions (GSP < 650 mm or GST < 23 °C), and CT was conducive to higher maize yield under good hydrothermal conditions (GSP > 650 mm or GST > 23 °C) . Besides, the average farm size was positively correlated with the CT total areas.

Optimum fertilizer application rate to ensure yield and decrease greenhouse gas emissions in rain-fed agriculture system of the Loess Plateau.

Application of nitrogen (N) can increase the supply of N in soil and, in turn, can lead to higher yield-but also to large increase in emissions of greenhouse gases (GHGs) if applied in excess. To determine the optimum dose of N for maize planting system, we analysed the relationship between yield and emissions of GHGs at seven levels of N, namely 50, 100, 150, 200, 250, 300, and 350 kg ha-1, using the DNDC (denitrification decomposition) model and maize grown with and without mulching. The model simulated the following variables: maize production; emissions of carbon dioxide (CO2), nitrous oxide (N2O), and methane (CH4); global warming potential (GWP); and GHG intensity (GHGI). We used data from 1980 to 2013 for a rain-fed region of the Loess Plateau in north-western China and validated the DNDC model against data from field experiments. The model performed well in simulating yield and GHG emissions (Adj.R2 > 0.61). Under mulching, the average yield of maize was 3.6-12.2 t ha-1 and the partial factor productivity was 73.1-35.0 kg kg-1; and both of these were significantly higher 78%-236% than those in the crop without mulching. The emissions of CO2, N2O, and the GWP increased with the increase in the dose of N whereas CH4 emissions remained unaffected by the dose. Mulching increased yields significantly in the north-western region, and the GWP and GHGI were higher mainly in the central and north-western regions. The optimum dose of N for maize grown with mulching ranged between 150 kg ha-1 and 200 kg ha-1 and offers the best balance between higher yield and lower emissions. The optimum dose may promote the development of mulched maize and provide a reference standard for dryland agriculture in zones with similar climates elsewhere in the world.

Three doses of an inactivation-based COVID-19 vaccine induces cross-neutralizing immunity against the SARS CoV-2 Omicron variant.

The immunity potency upon natural infection or vaccination is the main concern for the vaccine strategy of severe acute respiratory syndrome coronavirus 2 (SARS COV-2 variant), especially the recently reported Omicron variant (B.1.1.529). In this study, 200 recipients immunized with three doses of a COVID-19-inactivated vaccine were enrolled, whose serum samples were collected within 2 months after the third immunization. The neutralizing activity of sera against the pseudotyped Omicron variant, prototype, and Delta variant was determined. Our results demonstrated that the positive neutralization activity was 95.5% for the Omicron variant, 99.5% for the prototype, and 98.5% for the Delta variant. The geometric mean titers (GMT) for the Omicron variant was 49 and maintained sustained immune levels for 2 months, which decreased by 4.9-fold and 3.0-fold compared with the prototype (GMT, 239) and Delta variant (GMT, 148), respectively. In summary, our study demonstrated that three doses of a COVID-19-inactivated vaccine effectively yielded potent cross-neutralizing activity against the Omicron variant at 2 months after the third vaccination.

Spin Polarization Properties of Two Dimensional GaP3 Induced by 3d Transition-Metal Doping.

The electronic structure and spin polarization properties of monolayer GaP3 induced by transition metal (TM) doping were investigated through a first-principles calculation based on density functional theory. The calculation results show that all the doped systems perform spin polarization properties, and the Fe-doped system shows the greatest spin polarization property with the biggest magnetic moment. Based on the analysis from the projected density of states, it was found that the new spin electronic states originated from the p-d orbital couplings between TM atoms and GaP3 lead to spin polarization. The spin polarization results were verified by calculating the spin density distributions and the charge transfer. It is effective to introduce the spin polarization in monolayer GaP3 by doping TM atoms, and our work provides theoretical calculation supports for the applications of triphosphide in spintronics.

Quantitation of clevidipine in dog blood by liquid chromatography tandem mass spectrometry: application to a pharmacokinetic study.

Clevidipine, a vascular selective calcium channel antagonist of the dihydropyridine class, is rapidly metabolized by ester hydrolysis because of incorporation of an ester linkage into the drug molecule. To characterize its pharmacokinetic profiles in dogs, a simple, rapid and sensitive liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed and validated for quantitation of clevidipine in dog blood. After one-step protein precipitation with methanol, the chromatographic separation was carried out on an Ecosil C18 column (150mm×4.6mm, 5µm) with a gradient mobile phase consisting of methanol and 5mM ammonium formate at a flow rate of 0.5mL/min. The quantitation analysis was performed using multiple reaction monitoring (MRM) at the specific ion transitions of m/z 454.1 [M-H](-)→m/z 234.1 for clevidipine and m/z 256.1 [M-H](-)→m/z 227.1 for elofesalamide (internal standard) in the negative ion mode with electrospray ionization (ESI) source. This validated LC-MS/MS method showed good linearity over the range 0.5-100ng/mL with the lower limit of quantitation (LLOQ) of 0.5ng/mL together with the satisfied intra- and inter-day precision, accuracy, extraction recovery and matrix effect. Stability testing indicated that clevidipine in dog blood with the addition of denaturant methanol was stable on workbench for 1h, at -80°C for up to 30 days, and after three freeze-thaw cycles. Extracted samples were also observed to be stable over 24h in an auto-sampler at 4°C. The validated method has been successfully applied to a pharmacokinetic study of clevidipine injection to 8 healthy Beagle dogs following intravenous infusion at a flow rate of 5mg/h for 0.5h.