RESUMEN
In female sexually transmitted infection clinic attendees, Mycoplasma genitalium was more frequently detected using vaginal (53/73) versus endocervical (43/73) specimens. In women without other sexually transmitted infections, M. genitalium detection (N = 44) was associated with age ≤22 years (odds ratio, 2.53; P = 0.006) and clinical evidence of cervicitis (odds ratio, 2.11; P = 0.03).
Asunto(s)
Infecciones por Chlamydia/diagnóstico , Chlamydia trachomatis/aislamiento & purificación , Gonorrea/diagnóstico , Infecciones por Mycoplasma/diagnóstico , Mycoplasma genitalium/aislamiento & purificación , Enfermedades de Transmisión Sexual , Vaginitis por Trichomonas/diagnóstico , Cervicitis Uterina/diagnóstico , Adulto , Distribución por Edad , Cuello del Útero/microbiología , Infecciones por Chlamydia/epidemiología , Infecciones por Chlamydia/microbiología , Coinfección , Estudios Transversales , ADN Bacteriano/aislamiento & purificación , Inglaterra/epidemiología , Femenino , Gonorrea/epidemiología , Gonorrea/microbiología , Humanos , Infecciones por Mycoplasma/epidemiología , Infecciones por Mycoplasma/microbiología , Reacción en Cadena de la Polimerasa , Valor Predictivo de las Pruebas , Estudios Prospectivos , Enfermedades de Transmisión Sexual/diagnóstico , Enfermedades de Transmisión Sexual/epidemiología , Enfermedades de Transmisión Sexual/microbiología , Encuestas y Cuestionarios , Vaginitis por Trichomonas/epidemiología , Vaginitis por Trichomonas/microbiología , Cervicitis Uterina/epidemiología , Cervicitis Uterina/microbiología , Vagina/microbiología , Adulto JovenRESUMEN
Trichomoniasis is a common sexually transmitted disease associated with preterm birth, low birth weight, and increased susceptibility to infection with other pathogenic sexually transmitted microorganisms. Nucleic acid amplification tests for Trichomonas vaginalis have improved sensitivity for detecting infected individuals compared to existing culture-based methods. This prospective, multicenter U.S. clinical trial evaluated the performance of the automated Aptima T. vaginalis assay for detecting T. vaginalis in 1,025 asymptomatic and symptomatic women. Vaginal swab, endocervical swab, ThinPrep PreservCyt, and urine specimens were collected. Subject infection status was determined by wet-mount microscopy and culture. Aptima T. vaginalis assay performance was determined for each specimen type by comparison to subject infection status. Of 933 subjects analyzed, 59.9% were symptomatic. Aptima T. vaginalis clinical sensitivity and specificity were, respectively, 100% and 99.0% for vaginal swabs, 100% and 99.4% for endocervical swabs, 100% and 99.6% in ThinPrep samples, and 95.2% and 98.9% in urine specimens. Aptima T. vaginalis performance levels were similar in asymptomatic and symptomatic subjects. This study validates the clinical performance of the Aptima T. vaginalis assay for screening asymptomatic and symptomatic women for T. vaginalis infection.