RESUMEN
Survival of Onchocerca volvulus, a pathogenic human filarial parasite, is likely to depend upon the detoxification activities of the glutathione S-transferases (GSTs). The 24 kDa O. volvulus GST, OvGST2, was expressed in a bacterial system and the recombinant protein was purified to homogeneity by affinity chromatography. Specific activities of the recombinant OvGST2 (rOvGST2) with a variety of substrates, and in the presence of inhibitors, were determined. With the universal substrate 1-chloro-2,4-dinitrobenzene, the specific activity of rOvGST2 was 2130 nmol min-1 mg-1. The rOvGST2 showed relatively limited selenium-independent glutathione peroxidase activity, but secondary products of lipid peroxidation, namely members of the trans,trans-alka-2,4-dienal,trans-alk-2-enal and 4-hydroxyalk-2-enal series, were conjugated to glutathione via OvGST2 dependent activity. The gene encoding the OvGST2 was isolated and the nucleotide sequence determined. The ovgst2 gene was found to possess seven exons with six intervening sequences, with all except one having consensus splice-site junctions. This intron/exon organisation of the ovgst2 gene is almost identical with those described for the mammalian Pi class GST genes, consistent with the protein structural evidence that the OvGST2 is related to the Pi class GSTs. Southern blot analysis with total parasite genomic DNA indicated a single copy gene, with a restriction pattern consistent with that of the isolated gene. The tissue distribution of the OvGST2 was examined in O. volvulus by immunohistochemistry and was shown to be distinct from that of the OvGST1. The OvGST2 was located throughout the syncytial hypodermis of male and female adult worms, as well as in the uterine epithelium. Microfilariae, and infective third stage larvae of O. volvulus, isolated from Simulium neavei, were immunopositive for OvGST2.
Asunto(s)
Genes de Helminto , Glutatión Transferasa/genética , Glutatión Transferasa/metabolismo , Onchocerca volvulus/enzimología , Secuencia de Aminoácidos , Animales , Secuencia de Bases , ADN Complementario/genética , Exones , Femenino , Glutatión/metabolismo , Glutatión Transferasa/análisis , Glutatión Transferasa/química , Técnicas para Inmunoenzimas , Intrones , Peroxidación de Lípido , Masculino , Datos de Secuencia Molecular , Peso Molecular , Onchocerca volvulus/genética , Onchocerca volvulus/crecimiento & desarrollo , Proteínas Recombinantes/química , Proteínas Recombinantes/metabolismo , Especificidad por SustratoRESUMEN
Within the context of studies on the antioxidant enzymes in Onchocerca volvulus, DNA clones encoding catalase (CAT) were isolated from an O. volvulus adult lambda zapII cDNA library. Analysis of their nucleotide and encoded amino acid sequences revealed that they derive from intracellular bacteria, rather than the O. volvulus nuclear genome. The endobacterial CAT gene was found to lie in a gene cluster, followed by a ferritin gene and an excinuclease gene. The endobacterial CAT gene encodes a functional enzyme capable of detoxifying H2O2, demonstrated by producing an active recombinant protein in an E. coli expression system. The purified 54 kDa protein has CAT activity over a broad pH range, with a specific activity of 103,000 +/- 3000 U mg(-1). The optical spectrum of the endobacterial CAT shows that it is a ferric haem-containing protein with a Soret band at 405 nm. To investigate the phylogeny of the intracellular bacterium in O. volvulus, a segment of the 16S rRNA gene was amplified from total genomic DNA by a polymerase chain reaction using universal eubacterial primers. A phylogenetic analysis of the O. volvulus-derived 16S rRNA sequence revealed that the endobacterium belongs to a distinct Wolbachia clade of the order Rickettsiales. Onchocercomata and biopsies containing different onchocercal species were immunohistochemically stained using polyclonal antibodies raised against the recombinant endobacterial CAT. CAT was detected in the endobacteria in the hypodermis of adult male and female O. volvulus, O. ochengi, O. gibsoni and O. fasciata. The endobacterial enzyme was also detected in onchocercal oocytes and all embryonic stages including intrauterine microfilariae as well as skin microfilariae. O. volvulus thus harbours Wolbachia-like endosymbionts which are transovarially transmitted and show particular affinity for the hypodermal tissues of the lateral chords.
Asunto(s)
Catalasa/genética , Genes Bacterianos , Onchocerca volvulus/microbiología , Rickettsiaceae/genética , Secuencia de Aminoácidos , Animales , Catalasa/análisis , Catalasa/química , Catalasa/metabolismo , Femenino , Genes de ARNr , Técnicas para Inmunoenzimas , Masculino , Microfilarias/enzimología , Microfilarias/microbiología , Microscopía Electrónica , Datos de Secuencia Molecular , Onchocerca volvulus/enzimología , Onchocerca volvulus/crecimiento & desarrollo , Onchocerca volvulus/ultraestructura , Sistemas de Lectura Abierta , Filogenia , ARN Bacteriano/genética , ARN Ribosómico 16S/genética , Proteínas Recombinantes/química , Proteínas Recombinantes/metabolismo , Rickettsiaceae/enzimología , Rickettsiaceae/ultraestructura , Alineación de SecuenciaRESUMEN
This study describes the histological localization of two CuZn superoxide dismutases (SOD1 and SOD2) in the parasitic nematode Onchocerca volvulus, and a functional characterization of the 'extracellular' form of this enzyme (SOD2) which provides evidence that it is involved in the defense against environmental superoxide anion radicals. These essential enzymes are detected in larval and adult stages of the parasite, determined at the mRNA and protein levels by in situ hybridization and immunolocalization studies. These proteins are distributed throughout the worm, at various concentrations with particularly high levels produced in the hypodermis. In vitro maintenance of parasites indicated that SOD2 was secreted outside the parasite into the medium. Baculovirus constructs designed to test the ability of the SOD2 hydrophobic N-terminal region to function in processing and secretion confirmed the ability of this polypeptide sequence to direct the secretion of a marker protein, as well as of the mature SOD2 enzyme. Analyses of the native, mature SOD2 enzyme molecular mass, and the primary and quaternary structure, indicate that unlike other extracellular SODs, the SOD2 is active as a non-glycosylated dimer, rather than as a tetrameric glycoprotein. The detection of SOD2 outside of the parasite maintained in vitro, and the confirmation that the SOD2 is a secreted enzyme, indicate that this enzyme plays a role in the interactive biology of parasitic nematodes with their hosts.
Asunto(s)
Onchocerca volvulus/enzimología , Superóxido Dismutasa/metabolismo , Animales , Citosol/enzimología , Escherichia coli/genética , Espacio Extracelular/enzimología , Femenino , Regulación del Desarrollo de la Expresión Génica , Humanos , Inmunohistoquímica , Isoenzimas/genética , Isoenzimas/metabolismo , Masculino , Onchocerca volvulus/genética , Onchocerca volvulus/crecimiento & desarrollo , ARN de Helminto/genética , ARN de Helminto/metabolismo , ARN Mensajero/genética , ARN Mensajero/metabolismo , Proteínas Recombinantes/genética , Proteínas Recombinantes/metabolismo , Superóxido Dismutasa/genéticaRESUMEN
In onchocerciasis, variations of the host's immune responsiveness produce a spectrum of clinical manifestations ranging from the common generalized to the rare hyperreactive form (sowda). For further characterization of the immune response, the localization and frequency of mast cells in onchocercomas from untreated and ivermectin-treated patients with hyperreactive onchocerciasis from Liberia and the Yemen were analysed and compared to the generalized form by immunohistochemistry with antibodies specific for human mast cell tryptase and chymase, histamine and IgE. The nodules were selected with special regard to only one pair of live, microfilariae-producing Onchocerca volvulus. Throughout the nodular tissue of the hyperreactive form, mast cells accumulated in the strong inflammatory infiltrates, especially near eosinophils and around cellular attacks on microfilariae as well as perivascularly. Their number was significantly higher in the whole nodular tissue compared to the generalized form. The highest numbers occurred in the nodule centre. Mast cells carried IgE and appeared activated. No mast cells were observed in the cystic parts or attached to adult worms or microfilariae. In onchocercomas, 1 and 3 days after treatment with ivermectin, microgranuloma formation by eosinophils and macrophages around damaged microfilariae was enhanced and accompanied by numerous mast cells. Attacks of neutrophils were also pronounced, but attacks by mast cells were not observed. In conclusion, hyperreactivity against microfilariae in onchocercomas clearly correlates with a strong mastocytosis and IgE production parallel to tissue eosinophilia.
Asunto(s)
Inmunoglobulina E/análisis , Mastocitos/inmunología , Onchocerca volvulus/inmunología , Oncocercosis/inmunología , Enfermedades Cutáneas Parasitarias/inmunología , Adulto , Animales , Antiparasitarios , Recuento de Células , Niño , Quimasas , Femenino , Filaricidas/uso terapéutico , Granuloma , Histamina/análisis , Humanos , Ivermectina/uso terapéutico , Masculino , Microfilarias/inmunología , Persona de Mediana Edad , Oncocercosis/tratamiento farmacológico , Oncocercosis/patología , Serina Endopeptidasas/análisis , Enfermedades Cutáneas Parasitarias/patología , TriptasasRESUMEN
We have shown that fully differentiated cones in the salmon retina die as a result of apoptosis (normal cell death). These putative UV cones begin to disappear from the main retina when the fish is aged 120 days and are completely absent at day 220. However, they continue to be produced in the growth zones, ora serrata and ventral fissure, where they are shortlived and never incorporated into the main retina. The dying cones in the main retina and the growth zones are engulfed by macrophages and Müller cells.
Asunto(s)
Células Fotorreceptoras Retinianas Conos/ultraestructura , Salmón/anatomía & histología , Envejecimiento/fisiología , Animales , Apoptosis , Macrófagos/fisiología , Microscopía Electrónica , Fagocitosis , Retina/ultraestructura , Células Fotorreceptoras Retinianas Conos/fisiología , Rayos UltravioletaRESUMEN
The study describes the immunohistological localization of the extracellular CuZn superoxide dismutase (SOD2) in the parasitic nematode Onchocerca volvulus. Using specific antiserum raised against a 15-amino-acid peptide from the N-terminal region of the mature protein, this enzyme is detected primarily in the intestinal epithelium of the adult worms and to a lesser extent in the muscle cells of the uterine wall. A blocking experiment with the SOD2 peptide reduced the staining significantly, confirming specificity. The localization profile of SOD2 correlates extremely well with the localization of iron deposits in the gut and uterine muscle cells of adult O. volvulus. The detection of SOD2 in the functional intestine of O. volvulus, together with the evidence that it is a secreted protein, indicates that this enzyme in parasitic nematodes is in a position to interact with host molecules. It also demonstrates the accessibility of the parasite enzyme to an inhibitor or blocking antibody.
Asunto(s)
Onchocerca volvulus/enzimología , Superóxido Dismutasa/análisis , Secuencia de Aminoácidos , Animales , Especificidad de Anticuerpos , Células Epiteliales/química , Células Epiteliales/enzimología , Epítopos , Femenino , Compuestos Férricos/análisis , Hemosiderina/análisis , Sueros Inmunes/inmunología , Técnicas para Inmunoenzimas , Intestinos/química , Intestinos/enzimología , Masculino , Datos de Secuencia Molecular , Onchocerca volvulus/química , Superóxido Dismutasa/química , Superóxido Dismutasa/inmunología , Útero/enzimologíaRESUMEN
Onchocercomata with a single live or dead worm were analyzed to elucidate the infiltration of eosinophils. Females were classified according to the presence or absence of microfilariae in their uteri and in the nodular tissues. Immunohistochemical staining was performed using antibodies against eosinophil cationic protein, peroxidase, and major basic protein. Very few eosinophils were detected in nodules containing females without microfilariae or male or dead worms only, whereas eosinophils were abundant in all nodules with females producing microfilariae. The occurrence of eosinophils was not related to the age of the worm. Occasionally, degenerated or dead microfilariae attacked by activated eosinophils were found. By examination of onchocercomata with or without microfilariae from the same patient, it was excluded that the occurrence of eosinophils was dependent mainly on the host's immune status. In conclusion, live adult Onchocerca volvulus do not elicit an invasion of eosinophils as long as they do not produce microfilariae. The absence of eosinophilia does not exclude onchocerciasis.
Asunto(s)
Eosinófilos/parasitología , Onchocerca volvulus/aislamiento & purificación , Oncocercosis/parasitología , Animales , Eosinófilos/patología , Femenino , Humanos , Masculino , Microfilarias/aislamiento & purificación , Oncocercosis/patologíaRESUMEN
Glutathione S-transferases (GSTs) are essential detoxification enzymes for virtually all cells and may additionally aid in parasite survival by counteracting host-induced damage. GSTs from parasitic nematodes have been identified as potential targets for both immuno- and chemotherapy. To more closely characterize a 31-kDa (OvGST1) and a 24.5-kDa (OvGST2) GST from the pathogenic human filarial parasite Onchocerca volvulus, immunolocalization by electron microscopy was performed using two distinct affinity-purified polyclonal antisera raised against the recombinant OvGST1 and OvGST2. The strongest immunogold staining for OvGST1 was identified in the body wall of adult worms, especially in protuberances of the cuticle which lie in pouches of the hypodermis and in the outer zone of the syncytial hypodermis, where the external plasma membrane forms series of lamellae. Gold particles were also observed on the epicuticle of the adults and in the region of the border between the cuticle and hypodermis of microfilariae. The larval stages L1, L2, and infective L3 were also immunopositive for OvGST1. There was no specific labeling in the longitudinal musculature, the intestine, or the uterine wall of the adult worm. In contrast to the results for OvGST1, immunogold labeling for OvGST2 was observed throughout the whole hypodermal cytoplasm. The epithelial cells of the uterine wall showed moderate labeling. These ultrastructural immunolocalization results are consistent with the molecular characterization of both enzymes, indicating that OvGST1 is secreted out of the hypodermis into the cuticle and is acting at the host-parasite interface, while OvGST2 functions as an intracellular cytosolic housekeeping enzyme.
Asunto(s)
Glutatión Transferasa/análisis , Onchocerca volvulus/enzimología , Onchocerca volvulus/ultraestructura , Oncocercosis/parasitología , Animales , Femenino , Humanos , Inmunohistoquímica , Larva/enzimología , Larva/ultraestructura , Microscopía InmunoelectrónicaRESUMEN
Onchocercomata with a defined worm population were analysed to elucidate the distribution of mast cells. Nodules with live females were classified according to the presence or absence of microfilariae. Immunohistochemical staining was performed using antibodies specific for mast cells or IgE. Mast cells appeared singly or in diffuse accumulations perivascularly and in inflammatory infiltrates between adult Onchocerca volvulus and in the capsular area. No mast cells were detected in cystic parts. Only few, scattered mast cells were found in the fibrous zone around the adult worm. They were increased with stronger infiltration and hence, related to the inflammatory cells. Mast cells were never localized directly at adult worms or microfilariae. A correlation of the mast cell distribution to the occurrence of eosinophils was observed regarding higher numbers of mast cells and eosinophils in nodules with microfilariae-producing females. Nodules with single males revealed higher numbers of mast cells than nodules with non-producing females, although both contained very few eosinophils. Onchocercomata with dead worms contained significantly more mast cells than those with live filariae. In conclusion, the localization and frequency of mast cells is contingent on the vitality and productivity of the worms and therefore, indirectly and directly on the release of O. volvulus antigens.
Asunto(s)
Mastocitos/fisiología , Onchocerca volvulus/fisiología , Oncocercosis/patología , África , Fosfatasa Alcalina/química , Animales , Anticuerpos Monoclonales/inmunología , Recuento de Células , Eosinófilos/inmunología , Eosinófilos/fisiología , Femenino , Guatemala , Humanos , Procesamiento de Imagen Asistido por Computador , Inmunohistoquímica , Masculino , Mastocitos/inmunología , Microfilarias/inmunología , Microfilarias/fisiología , Onchocerca volvulus/inmunología , Oncocercosis/inmunología , Oncocercosis/parasitologíaRESUMEN
Onchocercal nodules were stained immunohistochemically using antibodies specific for human mast cells and IgE to elucidate the localization and frequency of mast cells after a single oral dose of 150 microg/kg ivermectin. Tryptase-and chymase-positive mast cells occurred predominantly in mixed inflammatory infiltrates and perivascularly, and never adhered to adult worms or microfilariae. Up to three days after ivermectin, mast cells and IgE-positive cells were markedly increased in the capsular area of nodules containing female worms with embryos and microfilariae compared to untreated nodules. In the centre of these nodules, around the adult Onchocerca volvulus, we found many tryptase-positive cells. More mast cells were IgE-positive than in untreated nodules, equalling the number of tryptase-positive mast cells. There was a clear correlation between the appearance of mast cells and the attacks on damaged microfilariae by eosinophils and macrophages and in the vicinity of adult worms by neutrophils that occur soon after ivermectin treatment. Onchocercomata harbouring female worms with oocytes only revealed, after all treatment intervals, the same mast cell numbers as untreated nodules. In conclusion, during the first three days after administration, ivermectin produces increased numbers of mast cells in nodules harbouring females with embryos and microfilariae, probably as part of an allergic reaction to the attacked microfilariae. Four to 19 days after ivermectin the number of mast cells in the entire nodule is no longer elevated.
Asunto(s)
Antihelmínticos/farmacología , Ivermectina/farmacología , Mastocitos/efectos de los fármacos , Onchocerca volvulus , Oncocercosis/tratamiento farmacológico , Animales , Femenino , Humanos , Inmunoglobulina E/inmunología , Ivermectina/uso terapéutico , Mastocitos/fisiología , Onchocerca volvulus/inmunología , Oncocercosis/inmunologíaRESUMEN
Lymph node and skin biopsies from Liberian patients with generalized and localized (sowda) onchocerciasis were studied 12-68 hours after oral administration of ivermectin at a single dose of 150 micrograms/kg body weight. Electron microscopic examination and immunohistochemical staining with antibodies against two different forms of eosinophil cationic protein (ECP EG1, ECP EG2), eosinophil peroxidase (EPO) and cationic leukocyte antigen (CLA) were performed. Following their disappearance from the skin, a large number of microfilariae was found in the regional lymph nodes. The lymph nodes from treated patients had over ten times more eosinophils compared to those from untreated persons with a peak of eosinophil density at 40-48 hours after treatment. Degenerating microfilariae in the lymph nodes were encircled by eosinophils, which showed positive immunostaining for ECP, EPO or CLA. Intra- and extracellular eosinophil granules revealed a great variation in their condition. In some specific granules a variety of structural alterations in the crystalloid cores occurred while in others different stages of deficiency in the matrix electron density were observed. The frequent necrosis of eosinophils in the immediate vicinity and at some distance from the microfilariae, with subsequent release of granules and the deposition of toxic cationic granule proteins onto the microfilarial cuticle during the eosinophil-parasite adherence reaction, demonstrated the function of these proteins in the ivermectin-reinforced killing of microfilariae in lymph nodes.
Asunto(s)
Proteínas Sanguíneas/análisis , Eosinófilos/efectos de los fármacos , Ivermectina/uso terapéutico , Ganglios Linfáticos/patología , Oncocercosis/tratamiento farmacológico , Ribonucleasas , Animales , Anticuerpos Monoclonales/inmunología , Proteínas en los Gránulos del Eosinófilo , Peroxidasa del Eosinófilo , Eosinófilos/enzimología , Eosinófilos/parasitología , Eosinófilos/ultraestructura , Antígenos HLA/análisis , Humanos , Inmunohistoquímica , Ivermectina/farmacología , Ganglios Linfáticos/parasitología , Ganglios Linfáticos/ultraestructura , Microfilarias/aislamiento & purificación , Microscopía Electrónica , Necrosis , Onchocerca/aislamiento & purificación , Oncocercosis/patología , Peroxidasas/análisis , Piel/parasitología , Piel/patologíaRESUMEN
Onchocerca volvulus is a pathogenic human filarial parasite which, like other helminth parasites, is capable of evading the host's immune responses by a variety of defense mechanisms which are likely to include the detoxification and repair mechanisms of the enzyme glutathione S-transferase (GST). In this study, we show that one of the previously described GSTs from O. volvulus appears to possess the characteristics of a secreted enzyme. When the complete O. volvulus GST1 (OvGST1) sequence presented here is compared with those of other GSTs, 50 additional residues at the N terminus are observed, the first 25 showing characteristics of a signal peptide. This is consistent with the N-terminal sequence data on the native mature enzyme which begins at amino acid 26, based on the deduced protein sequence from the cDNA. The native protein, without the signal peptide sequence, possesses a 24-amino-acid extension not present in other GSTs. The deduced amino acid sequence of the OvGST1 cDNA clone was shown to possess four potential N-glycosylation sites. Digestion of O. volvulus homogenate with endoglycosidase, followed by detection of OvGST1 with specific antibody, indicated that the enzyme possesses at least two N-linked oligosaccharide chains. Gel filtration of the Escherichia coli-produced recombinant OvGST1 showed that it is enzymatically active as a nonglycosylated dimer. OvGST1 is found in the media surrounding adult worms maintained in culture, indicating that, in vitro, this enzyme is released from the worm. The strongest immunostaining for OvGST1 was observed in the outer cellular covering of the adult worm body, the syncytial hypodermis, especially in the interchordal hypodermis, where the peripheral membrane forms a series of lamellae which run into the outer zone of the hypodermal cytoplasm.
Asunto(s)
Glutatión Transferasa/química , Onchocerca volvulus/enzimología , Secuencia de Aminoácidos , Animales , Secuencia de Bases , Cartilla de ADN/química , Femenino , Glutatión Transferasa/metabolismo , Glicoproteínas/química , Glicosilación , Datos de Secuencia Molecular , Peso Molecular , Onchocerca volvulus/anatomía & histología , Procesamiento Proteico-Postraduccional , Distribución TisularRESUMEN
In recent years, bovine Onchocerca species have been used as models for human onchocerciasis in drug screens. They have been suggested for immunology studies and evaluation of vaccine candidates. Therefore, mast cells and their association with other inflammatory cells were studied in five onchocercal species of cattle and deer using immunohistology. Intact mast cells occurred in large numbers in the capsule and septae of nodules, in fibrous tissue adjacent to nonnodular worms, and perivascularly. Inactive and, more frequency, activated and degranulating mast cells were observed within infiltrates in the nodule center or around nonnodular filariae. They were not detected in direct contact with the cuticle of adult worms or of microfilariae or among the macrophages, giant cells, and neutrophils forming the innermost layer around the worms. Eosinophils, but not mast cells, were obviously associated with microfilariae-producing females. The distribution, frequency, and activity of mast cells were similar for all five species and O. volvulus.
Asunto(s)
Ligamentos/patología , Ligamentos/parasitología , Mastocitos/patología , Onchocerca/aislamiento & purificación , Oncocercosis/patología , Animales , Bovinos , Enfermedades de los Bovinos , Tejido Conectivo/parasitología , Tejido Conectivo/patología , Ciervos , Femenino , Humanos , Inflamación , Oncocercosis/fisiopatología , Oncocercosis/veterinariaRESUMEN
A recombinant glutathione S-transferase (GST) (EC 2.5.1.18) from the parasitic nematode Ascaris suum (AsGST1) displays specific activity with a variety of model substrates and secondary products of lipid peroxidation. The AsGST1 interacts with a range of model inhibitors, haematin-related compounds, bile acids and anthelminthics. The reported variations in biochemical activity correlate with structural differences observed by homology modelling. Here, differences in the topography of the proposed substrate binding site between the AsGST1 and the host GSTs were identified. A rabbit polyclonal antiserum was raised against the glutathione-binding proteins of A. suum and specific antibodies against AsGST1 were affinity-purified using the recombinant protein. These antibodies were used to localize the AsGST1 in adult worms by immunohistochemical staining. The strongest immunostaining for AsGST1 was localized in the intestine in all worms examined. This suggests that the enzyme may be responsible for the metabolism of materials that are incorporated from the environment, as well as for molecules that are excreted or secreted from the parasite to the environment. It also demonstrates the accessibility of the enzyme to an inhibitor or blocking antibody. In addition, the structure and sequence of the gene encoding AsGST1 have been determined. Southern-blot analyses of the AsGST1 gene suggests that it is a single-copy gene. The nucleotide sequence analysis revealed that the gene is composed of four exons and three introns, and potential regulatory elements were identified in the 5' flanking sequence.