Asunto(s)
Hemoglobina Fetal/análisis , Hemoglobinas Anormales/análisis , Agar , Secuencia de Aminoácidos , Aminoácidos/análisis , Ácido Aspártico , Electroforesis de las Proteínas Sanguíneas , Celulosa , Cromatografía por Intercambio Iónico , Electroforesis en Papel , Electroforesis en Gel de Almidón , Etnicidad , Femenino , Glicina , Humanos , India , Recién Nacido , Malasia , Fragmentos de Péptidos/análisis , Embarazo , Tripsina , Cordón UmbilicalAsunto(s)
Hemoglobina Fetal/análisis , Hemoglobinas Anormales/análisis , Adulto , Alanina , Aminoácidos/análisis , Sangre , Cromatografía por Intercambio Iónico , Electroforesis en Gel de Almidón , Femenino , Hemoglobina Fetal/aislamiento & purificación , Variación Genética , Glutamatos , Hemoglobinas Anormales/aislamiento & purificación , Humanos , Recién Nacido , Lisina , Masculino , Péptidos/análisis , Conformación Proteica , Texas , Tripsina , Cordón UmbilicalAsunto(s)
Hemoglobina Fetal/análisis , Hemoglobinas Anormales/análisis , Adulto , Alanina , Secuencia de Aminoácidos , Aminoácidos/análisis , Cromatografía en Gel , Cromatografía por Intercambio Iónico , Electroforesis en Gel de Almidón , Inglaterra , Femenino , Glutamatos , Humanos , Recién Nacido , Jamaica , Lisina , Masculino , EmbarazoAsunto(s)
Aminoácidos/metabolismo , Hemoglobinas Anormales/metabolismo , Oxígeno/sangre , Adulto , Alanina , Secuencia de Aminoácidos , Electroforesis en Papel , Electroforesis en Gel de Almidón , Femenino , Edad Gestacional , Hemoglobinopatías/genética , Hemoglobinas/metabolismo , Hemoglobinas Anormales/análisis , Humanos , Concentración de Iones de Hidrógeno , Recién Nacido , Leucina , Londres , Biología Molecular , Mutación , Péptidos/análisis , Embarazo , Prolina , Unión Proteica , SerinaRESUMEN
A Black patient in whom an acute febrile illness was followed by a haemolytic crisis is presented. Investigation of the haemolytic anaemia showed the presence of an unstable haemoglobin which was found to be haemoglobin Leiden. Those members of the family who were available for investigation also had the unstable haemoglobin but showed no haemolysis. Radio-isotope studies on the patient confirmed haemolysis but showed that the spleen was not the main site of cell destruction.
Asunto(s)
Anemia Hemolítica/sangre , Hemoglobinas Anormales/análisis , Adulto , Población Negra , Electroforesis de las Proteínas Sanguíneas , Eritrocitos Anormales/patología , Hemoglobinas Anormales/genética , Humanos , Masculino , Linaje , Reticulocitos/patología , SudáfricaRESUMEN
The development of the HIV protease inhibitor saquinavir (Ro 31-8959) required a range of analytical methods for its measurement in biological fluids. This paper describes the development of isocratic, reverse-phase HPLC/UV methods for the routine measurement of plasma levels of the drug together with a more sensitive radioimmunoassay. The performance of the two assays is compared with that of an HPLC/MS/MS method previously published and has been shown to be satisfactory, with coefficients of variation of calibration standards and quality control samples within the usual outside limits of +/- 15%. The HPLC/UV method can be routinely applied for concentrations down to 10-20 ng/ml and a lower limit of quantification of 1 ng/ml from 1 ml of human plasma is possible. The radioimmunoassay was developed for the specific measurement of saquinavir concentrations in human, HIV-positive plasma samples and has a lower limit of quantification of 0.5-1.0 ng/ml. Some preliminary findings suggested that it might not be specific in rat plasma and no attempts have been made to quantify any nonclinical samples with this technique. If still greater sensitivity is required, recourse can be made to the HPLC/MS/MS assay.