Differential molecular response of maize and Johnson grass against maize dwarf mosaic virus and bermuda grass southern mosaic virus.

Maize dwarf mosaic virus (MDMV) and bermuda grass southern mosaic virus (BgSMV) is the most important cereal potyvirus in Iran. Expression of some key genes in maize plants susceptible and tolerant to MDMV or BgSMV and gene expression profile of MDMV and BgSMV compatible or incompatible to Johnson grass plants were studied. Time points of 1, 9, 24 and 72 h after inoculation with both viruses were investigated as well. By analyzing the expression of the genes, it was identified that in maize infected by MDMV and BgSMV, the transcript levels of the peroxiredoxin, GLP, SAM, NPR1 and chlorophyll a-b binding genes were significantly higher in the tolerant than in susceptible plants during the entire experiment. In the BgSMV inoculated Johnson grass plants, some of the genes related to plant defense responses including NPR1, peroxiredoxin and SAM had higher expression level than the Johnson grass plants inoculated by MDMV. Important genes in maize tolerance like NPR1 and MT-LP, were analyzed by trilinear decomposition analysis and genes clustering. The upregulated expression of genes at one-hour post inoculation showed that the plant response to viruses was activated at the early stage of infection. Keywords: MDMV; BgSMV; gene expression; quantitative real-time PCR; trilinear decomposition analysis.

Evaluation of biohydrogenation rate of canola vs. soya bean seeds as unsaturated fatty acids sources for ruminants in situ.

An experiment was conducted to study disappearance of C14 to C18 fatty acids, lag times and biohydrogenation (BH) rates of C18 fatty acids of ground soya bean and canola seeds in situ. Three ruminally fistulated Dallagh sheep were used to determine ruminal BH of unsaturated fatty acids (UFAs). Differences in the disappearance of fatty acids through the bags and lag times were observed between the oilseeds. We saw that the longer the incubation time of the oilseeds in the rumen, the lower the content of C18:2 and C18:3. Significantly higher lag times for both C18:2 and C18:3 were observed in ground canola compared to ground soya bean. BH rates of C18:2 and C18:3 fatty acids in soya bean were three times higher than those of canola. These results suggest that the fatty acid profile of fat source can affect the BH of UFAs by rumen micro-organisms. So that UFAs of canola had higher ability to escape from ruminal BH. It seems that fatty acid profile of ruminant products is more affected by canola seed compared to soya bean seed.

Study on the chemical and microbial composition and probiotic characteristics of dominant lactic acid bacteria in fermented poultry slaughterhouse waste.

This study aimed to evaluate the quality of fermented poultry slaughterhouse waste and isolate and identify the probiotic bacteria present in it. Poultry slaughterhouse waste samples supplemented with 5% starter culture and 0.2% propionic acid received one of four experimental treatments, which consisted of further supplementation with: (1) 10% molasses (M10); (2) 15% molasses (M15); (3) 10% molasses with 200ppm butylated hydroxytoluene (MB10); and (4) 15% molasses with 200ppm butylated hydroxytoluene (MB15). All experimental treatments (silages) were fermented for 6days with sampling before fermentation (day 1) and at the end of days 3 and 6. The pH of the silages decreased during the fermentation as it was lower on days 3 and 6 than day 1 (P<0.05). On day 6 of the experiment, the number of lactic acid bacteria in MB15 was significantly (P<0.05) higher than in MB10. The percentage of non-protein nitrogen increased (P<0.05) on days 3 and 6 of fermentation. The amount of malondialdehyde indicated that the peroxidation ratio decreased (P<0.05) with increasing the fermentation time. Among the 23 lactic acid bacteria detected in MB15, nine isolates (39.1%) belonged to the species Lactobacillus plantarum, ten (43.5%) belonged to the species Lactobacillus rhamnosus, and four (43.5%) belonged to the species Lactobacillus fermentum. The isolates LPL9, LRH25, and LFE26 were identified as the strain of L. plantarum, L. ramnosus, and L. fermentum, respectively, that demonstrated the best characteristics for use as a probiotic. The results of this experiment indicated that the fermentation is an advisable method for reusing the nutrients of poultry slaughterhouse waste and producing a probiotic supplement.