RESUMEN
This study aimed to elucidate some novel pharmacological activities of Lonicera japonica (Caprifoliaceae), which is widely used in Oriental folk medicine. The ethanolic extract of L. japonica (LJ) dose dependently inhibited chick chorioallantoic membrane angiogenesis. The antinociceptive activity of LJ was assessed using the acetic acid-induced constriction model in mice. LJ showed anti-inflammatory activity in two in-vivo models: the vascular permeability and air pouch models. LJ suppressed the production of nitric oxide via down-regulation of inducible nitric oxide synthase in lipopolysaccharide-stimulated RAW264.7 macrophage cells. However, LJ was unable to suppress induction of cyclooxygenase-2 in the stimulated macrophage cells. LJ decreased the reactive oxygen species level in the stimulated macrophage cells. In brief, the flowers of L. japonica possess potent anti-angiogenic and antinociceptive activities, in addition to anti-inflammatory activity, which partly supports its therapeutic efficacy.
Asunto(s)
Analgésicos/farmacología , Inhibidores de la Angiogénesis/farmacología , Lonicera/química , Extractos Vegetales/farmacología , Analgésicos/administración & dosificación , Analgésicos/aislamiento & purificación , Inhibidores de la Angiogénesis/administración & dosificación , Inhibidores de la Angiogénesis/aislamiento & purificación , Animales , Antiinflamatorios/administración & dosificación , Antiinflamatorios/aislamiento & purificación , Antiinflamatorios/farmacología , Embrión de Pollo , Membrana Corioalantoides/efectos de los fármacos , Membrana Corioalantoides/metabolismo , Ciclooxigenasa 2/efectos de los fármacos , Ciclooxigenasa 2/metabolismo , Modelos Animales de Enfermedad , Relación Dosis-Respuesta a Droga , Macrófagos/efectos de los fármacos , Macrófagos/metabolismo , Masculino , Medicina Tradicional de Asia Oriental , Ratones , Ratones Endogámicos ICR , Extractos Vegetales/administración & dosificación , Extractos Vegetales/aislamiento & purificación , Especies Reactivas de Oxígeno/metabolismoRESUMEN
UNLABELLED: ETHNOPHARMACOLGICAL RELEVANCE: The aerial parts of Saururus chinensis Baill. are used for the treatment of edema and inflammatory diseases in the Oriental folk medicine. AIM OF THE STUDY: This study aimed to elucidate anti-inflammatory and related activities of the ethanol extract (SC) of the dried aerial parts of Saururus chinensis Baill. MATERIALS AND METHODS: Anti-angiogenic and anti-nociceptive activities of SC were analyzed using the chick chorioallantoic membrane (CAM) assay and acetic acid-induced writhing response, respectively. Anti-inflammatory activity of SC was evaluated using acetic acid-induced vascular permeability, carrageenan-induced air pouch formation and analyses of nitrite content and induced nitric oxide synthase (iNOS) level in the macrophage cells. RESULTS: SC dose-dependently displayed a strong inhibition in the CAM angiogenesis. SC showed significant anti-nociceptive activity in the writhing model. The anti-inflammatory activity of SC was also assessed in the two in vivo models, such as vascular permeability and air pouch models in mice. SC suppressed production of nitric oxide and induction of iNOS and cyclooxygenase-2 in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages. CONCLUSION: The aerial parts of Saururus chinensis possess potent anti-angiogenic and anti-nociceptive activities in addition to anti-inflammatory activity, which partly supports its therapeutic efficacy.