RESUMEN
AIM: The aim of the current study was to evaluate the therapeutic and regenerative effects of MSCs derived exosomes in the treatment of type 1 DM and to compare its effects with MSCs themselves. The experiment was done on forty albino rats grouped as follows, group (1): Ten healthy rats, group (2): Ten induced type 1 DM rats, group (3): Ten induced type 1 DM rats received exosomes intraperitoneally, and group (4): Ten induced type 1 DM rats received MSCs intraperitoneally. Serum glucose and plasma insulin levels were assessed weekly. QRT-PCR was done to assess regeneration of pancreatic beta cells by measuring insulin, Pdx1, Smad2, Smad3 and TGFß genes. Additionally, histopathological and immune-histochemical examinations were done to confirm pancreatic tissue regeneration. RESULTS: Regarding the assessed genes (insulin, Pdx1, Smad2, Smad3 and Tgfß) gene expression in MSCs treated group showed significant increase compared to diabetic group (p value < 0.001) and gene expression in exosomes treated group was increased significantly compared to diabetic and MSCs treated groups (p value < 0.001). Histopathological and immune-histochemical examination revealed regeneration of pancreatic islets in both treated groups. CONCLUSION: MSCs Derived exosomes showed superior therapeutic and regenerative results than MSCs themselves.
Asunto(s)
Diabetes Mellitus Tipo 1/metabolismo , Exosomas/metabolismo , Células Madre Mesenquimatosas/metabolismo , Animales , Glucemia/análisis , Glucemia/metabolismo , Células Cultivadas , Diabetes Mellitus Experimental/metabolismo , Exosomas/química , Femenino , Proteínas de Homeodominio/metabolismo , Insulina/sangre , Insulina/metabolismo , Islotes Pancreáticos/química , Islotes Pancreáticos/metabolismo , Ratas , Proteína Smad1/metabolismo , Proteína Smad2/metabolismo , Transactivadores/metabolismoRESUMEN
In the original publication of the article, the reference citation style in the article was published incorrectly. The journal follows 'Name and Year' style for references. However, they were cited in numbering style incoherent to the references given in the Reference section which were placed in alphabetical order.
RESUMEN
Background: Non-alcoholic fatty liver disease is a major problem worldwide that needs non-invasive biomarkers for early diagnosis and treatment response assessment. We aimed to assess the correlation between circRNA-HIPK3 and miRNA-29a expression and its role as miRNA-29a sponge, as well as the correlation between circRNA-0046367 and miRNA-34a expression and its role as miRNA-34a sponge and their effect on regulation of the Wnt/ß catenin pathway, which may provide a new target for treatment of non-alcoholic steatohepatitis. Methods: the research was performed on 110 participants: group (I): fifty-five healthy donors served as controls and group (II): fifty-five patients with fatty liver pattern on abdominal ultrasound. Lipid profile and liver functions were assessed. RT-PCR was performed to assess the RNAs: circRNA-HIPK3, circRNA-0046367, miRNA-29a, miRNA-34a and Wnt mRNA gene expression. ELISA was performed to determine ß-catenin protein levels. Results: miRNA-34a and circRNA-HIPK3 expression were significantly greater, while miRNA-29a and circRNA-0046367 expression were significantly less, in patients than in controls. Wnt/ß-catenin regulated by miRNA-29a and miRNA-34a showed a significant decrease that leads to its abnormal effect on lipid metabolism. Conclusions: our results imply that miRNA-29a can be investigated as a target for circRNA-HIPK3, while miRNA-34a can be investigated as a target for circRNA-0046367, and that circRNA-HIPK3 and circRNA-0046367 may have emerging roles that can affect the pathogenesis of nonalcoholic steatohepatitis through the Wnt/ß-catenin pathway and thus be used as therapeutic targets for the disease.