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BACKGROUND: In recent years, the safety and effectiveness of one-stage percutaneous nephrolithotomy (PCNL) for the treatment of calculous pyonephrosis have been proven. In order to further reduce postoperative pain and hospital stay, we first proposed and practiced the idea of one-stage tubeless percutaneous nephrolithotomy for calculous pyonephrosis. METHODS: A retrospective analysis was performed of case data of 30 patients with asymptomatic calculous pyonephrosis treated in our center with one-stage PCNL from January 2016 to January 2021. Patients were routinely given 20 mg of furosemide and 10 mg of dexamethasone sodium phosphate injection intravenously at the beginning of anesthesia. Among them, 27 patients successfully underwent one-stage tubeless percutaneous nephrolithotomy, while 3 cases were given indwelling nephrostomy tubes because of proposed second-stage surgery or the number of channels was greater than or equal to 3. All patients were operated on by the same surgeon. RESULTS: Preoperatively, 11 of 30 patients (8 men and 22 women) had positive urine bacterial cultures, and all were given appropriate antibiotics based on drug sensitivity tests. All patients completed the surgery successfully. The mean operative time was 66.6 ± 34.7 min, the mean estimated blood loss was 16.67 ± 14.34 mL and the mean postoperative hospital stay was 5.0 ± 3.1 days. The mean postoperative hospital stay was 4.6 ± 2.5 days among the 27 patients with one-stage tubeless percutaneous nephrolithotomy. Of the 3 patients with postoperative fever, 2 had the tubeless technique applied. One patient with 3 channels was given renal artery interventional embolization for control of postoperative bleeding. None of the 30 patients included in the study developed sepsis. The final stone-free rate was 93.3% (28/30) on repeat computed tomography at 1 month postoperatively. The final stone-free rate was 92.6% in the 27 patients undergoing one-stage tubeless percutaneous nephrolithotomy (25/27). CONCLUSIONS: One-stage tubeless PCNL is an available and safe option in carefully evaluated and selected calculous pyonephrosis patients.
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Cálculos Renales/cirugía , Nefrolitotomía Percutánea/métodos , Pionefrosis/cirugía , Adulto , Femenino , Humanos , Cálculos Renales/complicaciones , Masculino , Persona de Mediana Edad , Pionefrosis/complicaciones , Estudios Retrospectivos , Resultado del TratamientoRESUMEN
A TMT (Tandem Mass Tag)-based strategy was applied to elucidate proteins that change in proteomes of grouper fillets during refrigerated storage. In addition, quality analyses on pH, centrifugal loss, color (L *, a *, b *) and texture (hardness, chewiness, and gumminess) for grouper fillets were performed. A total of 64 differentially significant expressed proteins (DSEPs) were found in the results in the Day 0 vs. Day 6 group comparison and the Day 0 vs. Day 12 group comparison. It is worth mentioning that more proteome changes were found in the Day 0 vs. Day 12 comparisons. Bioinformatics was utilized to analyze the DSEP. UniProt Knowledgebase (UniProtKB), Gene Ontology (GO) enrichment, Kyoto Encyclopedia of Genes and Genomes (KEGG) and protein interaction network analysis were adopted. All DSEPs were classified into seven areas by function: binding proteins, calcium handling, enzymes, heat shock protein, protein turnover, structural proteins and miscellaneous. The numbers of proteins that correlated closely with pH, centrifugal loss, color (L *, a *, b *) and texture (hardness, chewiness, and gumminess) were 4, 3, 6 and 8, respectively.
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Peces , Conservación de Alimentos , Carne/análisis , Proteínas/química , Proteómica , Refrigeración , Espectrometría de Masas en Tándem , Animales , Biología Computacional/métodos , Análisis de los Alimentos , Ontología de Genes , Proteínas/genética , Proteoma , Proteómica/métodosRESUMEN
RATIONALE: Indwelling ureteral catheter, double J tube, or nephrostomy tube are routine procedures for percutaneous nephrolithotomy (PCNL) in children. There are a few cases in which PCNL has been performed in children without any additional instruments being left in place. PATIENT CONCERNS: In this study, 3 children were treated for hematuria and complicated with different degrees of urinary tract infection. All of them were diagnosed as upper urinary tract calculi by abdominal computed tomography. DIAGNOSIS: Three preschoolers were diagnosed with upper urinary tract calculi before surgery, one with no hydronephrosis and the other 2 with different degrees of hydronephrosis. INTERVENTIONS: After preoperative evaluation, all the children successfully completed PCNL without indwelling ureteral catheter, double J tube, or nephrostomy tube. OUTCOMES: The operation was successful and there were no residual stones observed during postoperative review. The operating times for the children were 33 minutes, 17 minutes, and 20 minutes, and the intraoperative bleeding volumes were 1 mL, 2 mL, and 2 mL. The catheter was removed on the second day after the operation, the postoperative review of the abdominal computed tomography or ultrasound did not indicate any stone residue, and there were no fever, bleeding, and other related complications after the operation. LESSONS: We believe that total tubeless PCNL without artificial hydronephrosis can be achieved in the preschool population.
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Hidronefrosis , Cálculos Renales , Nefrolitotomía Percutánea , Nefrostomía Percutánea , Uréter , Humanos , Preescolar , Cálculos Renales/cirugía , Tiempo de Internación , Nefrostomía Percutánea/métodos , Hidronefrosis/etiología , Hidronefrosis/cirugía , Hemorragia , Resultado del TratamientoRESUMEN
In digital subtraction angiography (digital subtraction total cerebral angiography), cardiac and macrovascular cardiography, anorectal radiology, fluoroscopy, and computed tomography (CT), a prior knowledge to X-ray energy spectrum is crucial for assessing the image quality and also calculating patient X-ray dosage. The present investigation's main objective is to propose an intelligent technique for faster calculating X-ray energy spectrum of medical imaging systems with different exposure settings of tube voltage, filter material, and thickness based on limited specific spectra. In this study, Monte Carlo N Particle (MCNP) simulation code was initially used to generate some limited X-ray spectra for tube voltages of 20, 30, 40, 50, 80, 100, 130, and 150 kV for two different filters of beryllium and aluminum with thicknesses of 0. 4, 0.8, 1.2, 1.6 and 2 mm. Tube voltage, type, and thickness of filter were used as the 3 inputs of 150 Radial Basis Function Neural Network (RBFNN) to forecast point by point of the X-ray spectrum. After training, the RBFNNs could forecast most of the X-ray spectra for tube voltages in the range of 20-150 kV and two various filters of aluminum and beryllium with thicknesses in the range of 0-2 mm.
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Aluminio , Berilio , Humanos , Rayos X , Radiografía , Redes Neurales de la Computación , Fantasmas de Imagen , Dosis de Radiación , Método de MontecarloRESUMEN
The air kerma, which is the amount of energy given off by a radioactive substance, is essential for medical specialists who use radiation to diagnose cancer problems. The amount of energy that a photon has when it hits something can be described as the air kerma (the amount of energy that was deposited in the air when the photon passed through it). Radiation beam intensity is represented by this value. Hospital X-ray equipment has to account for the heel effect, which means that the borders of the picture obtain a lesser radiation dosage than the center, and that air kerma is not symmetrical. The voltage of the X-ray machine can also affect the uniformity of the radiation. This work presents a model-based approach to predict air kerma at various locations inside the radiation field of medical imaging instruments, making use of just a small number of measurements. Group Method of Data Handling (GMDH) neural networks are suggested for this purpose. Firstly, a medical X-ray tube was modeled using Monte Carlo N Particle (MCNP) code simulation algorithm. X-ray tubes and detectors make up medical X-ray CT imaging systems. An X-ray tube's electron filament, thin wire, and metal target produce a picture of the electrons' target. A small rectangular electron source modeled electron filaments. An electron source target was a thin, 19,290 kg/m3 tungsten cube in a tubular hoover chamber. The electron source-object axis of the simulation object is 20° from the vertical. For most medical X-ray imaging applications, the kerma of the air was calculated at a variety of discrete locations within the conical X-ray beam, providing an accurate data set for network training. Various locations were taken into account in the aforementioned voltages inside the radiation field as the input of the GMDH network. For diagnostic radiology applications, the trained GMDH model could determine the air kerma at any location in the X-ray field of view and for a wide range of X-ray tube voltages with a Mean Relative Error (MRE) of less than 0.25%. This study yielded the following results: (1) The heel effect is included when calculating air kerma. (2) Computing the air kerma using an artificial neural network trained with minimal data. (3) An artificial neural network quickly and reliably calculated air kerma. (4) Figuring out the air kerma for the operating voltage of medical tubes. The high accuracy of the trained neural network in determining air kerma guarantees the usability of the presented method in operational conditions.
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Understanding the status and changes of plant diversity in rubber (Hevea brasiliensis) plantations is essential for sustainable plantation management in the context of rapid rubber expansion in the tropics, but remains very limited at the continental scale. In this study, we investigated plant diversity from 10-meter quadrats in 240 different rubber plantations in the six countries of the Great Mekong Subregion (GMS)-where nearly half of the world's rubber plantations are located-and analyzed the influence of original land cover types and stand age on plant diversity using Landsat and Sentinel-2 satellite imagery since the late 1980s. The results indicate that the average plant species richness of rubber plantations is 28.69 ± 7.35 (1061 species in total, of which 11.22 % are invasive), approximating half the species richness of tropical forests but roughly double that of the intensively managed croplands. Time-series satellite imagery analysis revealed that rubber plantations were primarily established in place of cropland (RPC, 37.72 %), old rubber plantations (RPORP, 27.63 %), and tropical forests (RPTF, 24.12 %). Plant species richness in RPTF (34.02 ± 7.62) was significantly (p < 0.001) higher than that in RPORP (26.41 ± 7.02) and RPC (26.34 ± 5.37). More importantly, species richness can be maintained for the duration of the 30-year economic cycle, and the number of invasive species decreases as the stand ages. Given diverse land conversions and changes in stand age, the total loss of species richness due to rapid rubber expansion in the GMS was 7.29 %, which is far below the traditional estimates that only consider tropical forest conversion. In general, maintaining higher species richness at the earliest stages of cultivation has significant implications for biodiversity conservation in rubber plantations.
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Hevea , Goma , Bosques , Biodiversidad , Especies IntroducidasRESUMEN
Retention of ureteral catheter to establish artificial hydronephrosis is a routine step of percutaneous nephrolithotomy procedure, which can improve the success rate of puncture, but it can prolong the procedure time and increase the risk of postoperative infection, especially for immunocompromised elderly patients. Therefore, this study aims to investigate the safety and effectiveness of ultrasound-guided percutaneous nephrolithotomy without indwelling ureteral catheter for older patients with upper urinary calculi. The clinical data of 119 older patients admitted to the Affiliated Hospital of Jining Medical University for percutaneous nephrolithotomy from January 2019 to December 2021 were retrospectively analyzed. The patients were divided into study and control groups according to whether the physician decided to use ureteral catheter during the procedure, and the differences in the success rate of one-time puncture, operative time, single-stage stone removal rate, postoperative hospital stay, and complication rate were compared between the 2 groups. There were no significant differences in the success rate of one-time puncture and single-stage stone removal rate between the 2 groups (Pâ >â .05). The operation time were significantly shorter in the study group [(30.0-61.0) minute vs (54.8-106.8) minute, Pâ =â .00], and the intraoperative bleeding was less in the study group [(5-20) mL vs (10-30) mL, Pâ =â .03]. The postoperative hospital stay was shorter in the study group [(2.5-4.0) days vs (3.0-5.0) days, Pâ =â .00], and the medical expenses were lower in the study group [(17,309.5-22,652.7) yuan vs (19,148.0-24,407.6) yuan, Pâ =â .02]. The incidence of systemic inflammatory response syndrome was lower in the study group (3.5% vs 19.4%, Pâ =â .007). There were no statistically significant differences in complications such as postoperative fever, renal artery embolism and blood transfusion between the two groups (Pâ >â .05). Ultrasound-guided percutaneous nephrolithotomy without indwelling ureteral catheter for elderly patients with upper urinary stones is safe and feasible.
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Cálculos Renales , Nefrolitotomía Percutánea , Nefrostomía Percutánea , Cálculos Ureterales , Cálculos Urinarios , Humanos , Anciano , Nefrolitotomía Percutánea/efectos adversos , Estudios Retrospectivos , Nefrostomía Percutánea/métodos , Catéteres Urinarios , Ultrasonografía Intervencional , Cálculos Renales/cirugía , Resultado del Tratamiento , Cálculos Ureterales/cirugíaRESUMEN
The binding between ß-lactoglobulin and epigallocatechin gallate (EGCG) under the pressure of 600 MPa was explored using molecular docking and molecular dynamics (MD) simulation. EGCG bound mainly in two regions with site 1 in internal cavity of the ß-barrel and site 2 on the surface of protein. 150 ns MD was performed starting from the structure with the optimal binding energy at the two sites in molecular docking, respectively. It was found that the protein fluctuated greatly when small molecule bound to site 2 at 0.1 MPa, and the protein fluctuation and solvent accessible surface area became smaller under high-pressure. The binding of small molecules made the protein structure more stable with increasing of α-helix and ß-sheet, while high-pressure destroyed α-helix of protein. The binding energy of small molecules at site 1was stronger than that at site 2 under 0.1 MPa, with stronger van der Waals and hydrophobic interaction at site 1 while more hydrogen bonds were present at site 2. The binding energy of both sites weakened under high-pressure, especially at site 1, causing the binding force to be weaker at site 1 than that at site 2 under high-pressure.
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Catequina/análogos & derivados , Lactoglobulinas/química , Simulación de Dinámica Molecular , Catequina/química , Humanos , Presión , Conformación Proteica en Hélice alfa , Conformación Proteica en Lámina betaRESUMEN
Grouper is an important seafood species in China and has high economic value. However, the edible value of grouper is seriously affected by deterioration in the texture and flavor during refrigeration. The purpose of this study was to investigate the effects of endogenous cathepsin and microorganisms on texture softening and flavor changes in refrigerated grouper fillets. Iodoacetic acid and ProClin 300 were used to inhibit endogenous protease activity and microbial growth separately. Iodoacetic acid can inhibit the activity of cathepsin B, L, and calpain. Moreover, iodoacetic acid does not significantly affect the growth of microorganisms. The total amounts of bacteria and Pseudomonas spp. in the samples treated with ProClin 300 were less than 2 log CFU g-1 and 1 log CFU g-1 on the 18th day, and the activity of protease was not significantly affected. On the 6th day, the hardness of the iodoacetic acid treatment group decreased by 8%, while the ProClin 300 treatment group decreased by 28%, and changes in the free amino acids and volatile substances significantly exceeded those of the iodoacetic acid treatment group, indicating that endogenous protease was the main factor in the texture deterioration. A first-order exponential decay model indicated that cathepsin L was the most important protease for reducing the hardness of grouper fillets, and changes in the content of free amino acids and volatile substances indicated that microorganisms played a more important role in the deterioration of flavor substances compared to that played by endogenous protease.
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DNA-binding with one finger (Dof) proteins comprise a plant-specific transcription factor family involved in plant growth, development and stress responses. This study presents a genome-wide comparison of Dof family genes in physic nut (Jatropha curcas) and castor bean (Ricinus communis), two Euphorbiaceae plants that have not experienced any recent whole-genome duplication. A total of 25 or 24 Dof genes were identified from physic nut and castor genomes, respectively, where JcDof genes are distributed across nine out of 11 chromosomes. Phylogenetic analysis assigned these genes into nine groups representing four subfamilies, and 24 orthologous groups were also proposed based on comparison of physic nut, castor, Arabidopsis and rice Dofs. Conserved microsynteny was observed between physic nut and castor Dof-coding scaffolds, which allowed anchoring of 23 RcDof genes to nine physic nut chromosomes. In contrast to how no recent duplicate was present in castor, two tandem duplications and one gene loss were found in the Dof gene family of physic nut. Global transcriptome profiling revealed diverse patterns of Jc/RcDof genes over various tissues, and key Dof genes involved in flower development and stress response were also identified in physic nut. These findings provide valuable information for further studies of Dof genes in physic nut and castor.
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This study investigates the effect of different packaging methods-namely, air packaging (AP), vacuum packaging (VP), and modified atmosphere packaging (MAP)-on the protein oxidation and degradation of grouper (Epinephelus coioides) fillets during refrigerated storage. The carbonyl group, myofibril fragmentation index, free amino acids, FTIR of myofibrillar proteins, and total protein SDS-PAGE were determined. The results showed that the protein oxidation degree of the fillets gradually increased as the storage time increased. The FTIR results indicated that the secondary structure transformed from an α-helix to an irregular curl. SDS-PAGE confirmed the degradation of the myosin heavy chain, and that myosin gradually occurred during refrigerated storage. Meanwhile, protein oxidation and degradation were highly correlated. Protein degradation was accelerated by protein oxidation in myofibrils, which included the increase of protein surface hydrophobicity and changes of the secondary structure. In fact, the protein oxidation and degradation of the grouper fillets were effectively inhibited by MAP and VP during refrigerated storage, and MAP (30% N2 and 70% CO2) had the best results.
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Dof proteins comprise a plant-specific transcription factor family involved in regulation of many plant processes. Despite their diverse roles, little information is available in cassava (Manihot esculenta, Euphorbiaceae), the sixth major staple crop in the world. This study presents the first genome-wide identification of Dof family genes in cassava, resulting in 45 family members that are unevenly distributed across 17 out of the 18 chromosomes. Phylogenetic analysis assigned these genes into eight groups representing four subfamilies. Synteny analysis and BRH (Best Reciprocal Hit) method were also adopted to study evolutionary relationships. Among 21 MeDof duplicates identified, the majority of them were derived from the recent whole-genome duplication (WGD), whereas only one from local duplication. Transcriptional profiling revealed diverse expression patterns among various tissues, and further comparison of conserved motifs and expression profiles revealed divergence of MeDof duplicates. These findings provide valuable information for functional analysis of Dof genes in cassava.
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Cromosomas de las Plantas/genética , Regulación de la Expresión Génica de las Plantas , Genoma de Planta , Estudio de Asociación del Genoma Completo , Manihot/genética , Familia de Multigenes , Proteínas de Plantas/genética , Secuencia de Aminoácidos , Perfilación de la Expresión Génica , Manihot/crecimiento & desarrollo , Filogenia , Homología de SecuenciaRESUMEN
OBJECTIVE: To explore effective approaches of treating elderly patients with distal tibiofibular fractures. METHODS: From August 2008 to October 2012,175 elderly patients with distal tibiofibular fractures were treated with locking compression plate (LCP) through anterior tibial. There were 112 males and 63 females with an average of 71.3 (ranged 60 to 83) years old. Of them,89 cases were treated by anterior tibial tension reduced incision with LCP,including 62 males and 27 females with a mean age of (71.8 +/- 6.4) years old. Eighty-six patients were treated by distal tibial incision with LCP,including 58 males and 28 females with a mean age of (70.3 +/- 6.7) years old. Swelling time, operation time, intraoperative blood loss, hospital stay, healing time, complications and AOFAS scores were compared between two groups after operation. RESULTS: Swelling time in anterior tension reduced incision with LCP and distal tibial incision with LCP was (5.6 +/- 1.3) and (9.7 +/- 2.1) days, healing time was (4.2 +/- 1.4) and (5.4 +/- 1.9) months,and complications were found 3 in tension reduced incision and 10 in distak tibial incision respectively;and all data shown statistically significant differences between two groups (P < 0.05). At 12 months after operation,AOFAS score was 89.0 +/- 9.7, 87.9 +/- 9.4; and there was no statistically significant difference between two groups (P > 0.05). CONCLUSION: Tension reduced incision through anterior tibial combined with locking compression plate fixation in treating elderly patients with distal tibiofibular fractures can provide good clinical effects with quick fracture healing and low complications.