RESUMEN
Semi-arid region of Northwest China is characterized by barren soil, with high pH and high carbonate content. Saline-alkali land is easily formed in this area, which inhibits soil microbial activity and results in soil nutrient loss. To improve soil health in this area, effects of intercropping alfalfa and triticale on rhizosphere soil nutrients and bacterial community structure were examined though a three-consecutive-year experiment. The results showed that soil organic matter (SOM) content of alfalfa rhizosphere in intercropping was significantly higher than that in monoculture, while pH and available potassium (AK) content were lower than that in monoculture. The concentrations of SOM, available nitrogen (AN), available phosphate (AP) and AK of triticale rhizosphere in intercropping were significantly higher than those in monoculture. The bacterial sequences, OTUs, richness, and diversity of alfalfa and triticale in rhizosphere soil were all higher than those in monoculture. At phylum level, Proteobacteria and Bacteroidetes were dominant, accounting for 31.1%-33.4% and 22.4%-32.2% of the total OTUs, respectively. The relative abundance of Bacteroidete was higher in intercropping than in monoculture. Actinomycetes in the monoculture were significantly higher. Acidobacteria in the intercropping triticale was significantly higher than that in monoculture triticale. Verrucomicrobia in intercropping alfalfa was significantly more abundant than that in monoculture of alfalfa. At genus level, Algoriphagus, Flavobacterium, Gp6, and Chryseolinea in monoculture were greater than those in intercropping, and Cellvibrio showed an opposite trend. Results of correlation analysis showed that the abundance of Proteobacteria and Bacteroidetes were negatively correlated with soil pH, while that of Bacteroidetes were positively correlated with SOM, TN and AN. However, the abundance of Chloroflexi, Planctomycetes, Acidobacteria, Verrucomicrobia, and Gemmatimonadetes was negatively correlated with SOM and AN. Overall, alfalfa-triticale intercropping is an effective strategy to improve soil health in semi-arid region of Northwest China.
Asunto(s)
Rizosfera , Triticale , Agricultura , China , Medicago sativa , Nutrientes , Suelo , Microbiología del SueloRESUMEN
AIM: To explore the changes of X-box binding protein 1 splicing (XBP1s) and inflammatory cytokine expression in patients with ulcerative colitis (UC) in response to endoplasmic reticulum stress (ERS). METHODS: Reverse transcription polymerase chain reaction and quantitative polymerase chain reaction were performed to detect the forms of XBP1s and the expression of interleukin (IL)-2, interferon (IFN)-γ, and IL-17α. Differences between patients with UC and normal subjects were then determined. RESULTS: Mononuclear cells of the peripheral blood of normal subjects and UC patients with were stimulated with no drugs (control), phytohemagglutinin (PHA), thapsigargin (TG), or both PHA and TG. XBP1s in patients with UC exhibited splicing, which was greater with co-stimulation than single stimulation. Co-stimulation increased the expression level of IL-2, IFN-γ, and IL-17α. CONCLUSION: The T lymphocytes of both normal subjects and patients with UC responded to ERS by activating the XBP1s-mediated signalling pathway, upregulating the expression of inflammatory cytokines, and increasing the occurrence of inflammation. The mononuclear cells in the peripheral blood of patients with UC were more sensitive to ERS than those in the peripheral blood of normal subjects.