Multi-band reprogrammable phase-change metasurface spectral filters for on-chip spectrometers.

Active optical metasurfaces provide a platform for dynamic and real-time manipulation of light at subwavelength scales. However, most active metasurfaces are unable to simultaneously possess a wide wavelength tuning range and narrow resonance peaks, thereby limiting further advancements in the field of high-precision sensing or detection. In the paper, we proposed a reprogrammable active metasurface that employs the non-volatile phase change material Ge2Sb2Te5 and demonstrated its excellent performance in on-chip spectrometer. The active metasurfaces support magnetic modes and feature Friedrich-Wintgen quasi bound states in the continuum, capable of achieving multi-resonant near-perfect absorption, a multilevel tuning range, and narrowband performance in the infrared band. Meanwhile, we numerically investigated the coupling phenomenon and the intrinsic relationship between different resonance modes under various structural parameters. Furthermore, using the active metasurfaces as tunable filters and combined with compressive sensing algorithms, we successfully reconstructed various types of spectral signals with an average fidelity rate exceeding 0.99, utilizing only 51 measurements with a single nanostructure. A spectral resolution of 0.5 nm at a center wavelength 2.538 µm is predicted when the crystallization fractions of GST change from 0 to 20%. This work has promising potential in on-site matter inspection and point-of-care (POC) testing.

Treatments of orange-spotted grouper (Epinephelus coioides) against Cryptocaryon irritans with •OH, ClO2 or HCHO: Survival, physiological and histological response.

Cryptocaryon irritans causes one of the most serious diseases in various wild and cultured marine fish, leading to mass mortality and economic loss. In this study, hydroxyl radical (•OH) solution produced by strong ionization discharge combined with water jet cavitation effect was injected into orange-spotted grouper (Epinephelus coioides) aquaculture tanks for C. irritans control. The results showed that all C. irritans theronts were inactivated by •OH solution at concentrations of 0.5 mg/L within 2 min. •OH could induce alteration of shape, the absence of motility and macronucleus dispersion in theronts. A possible explanation was that the macronucleus of C. irritans might be damaged by •OH; as a result, its metabolism and life activities were disturbed. The •OH treatment increased the survival rate of E. coioides challenged with C. irritans from 64.7 ± 8.0% (mean ± SD) to 100% and reduced their infection intensity significantly. Stress response biomarkers such as malonaldehyde, glutathione, glutathione peroxidase, superoxide dismutase (SOD) and catalase levels in the gills of E. coioides at different time points were analysed. The SOD activity in the •OH group first decreased and then recovered to the initial level at the end of the experiment. The other stress response biomarkers had no significant difference from that in the uninfected control group after •OH treatment. Additionally, the gill of E. coioides in the •OH group exhibited slight and reversible transformation compared with the uninfected control group. Compared with •OH treatment, chlorine dioxide and formalin treatment reduced the survival rate, induced oxidative damage and changed the histological gill structure in E. coioides. In conclusion, •OH could be applied effectively to control C. irritans infection without affecting the normal physiological condition of E. coioides.

Optimization of quantum light sources and four-wave mixing based on a reconfigurable silicon ring resonator.

Being a key component on a photonic chip, the microring usually specializes in a certain nonlinear optical process and can not simultaneously meet different working conditions for different processes. Here, we theoretically and experimentally investigate a reconfigurable silicon microring resonator to act as a optimization strategy for both classical four-wave mixing and quantum light sources. Experimental results show that the four-wave mixing efficiency with continuous wave and pulsed pump can be both optimized to a high value well matching numerical analysis. A variety of quantum light sources - including the heralded single-photon source, two-photon source and multi-photon source - are demonstrated to present a high performance and their key parameters including the pair generation rates (PGR), the heralding efficiency (HE) and the coincidence-to-accidental ratio (CAR) are controllable and optimizable. Such tunable nonlinear converter is immune to fabrication variations and can be popularized to other nonlinear optical materials, providing a simple and compact post-fabrication trimming strategy for on-chip all-optical signal processing and photonic quantum technologies.

Plasmonic Near-Infrared Photoconductor Based on Hot Hole Collection in the Metal-Semiconductor-Metal Junction.

Harvesting energetic carriers from plasmonic resonance has been a hot topic in the field of photodetection in the last decade. By interfacing a plasmonic metal with a semiconductor, the photoelectric conversion mechanism, based on hot carrier emission, is capable of overcoming the band gap limitation imposed by the band-to-band transition of the semiconductor. To date, most of the existing studies focus on plasmonic structural engineering in a single metal-semiconductor (MS) junction system and their responsivities are still quite low in comparison to conventional semiconductor, material-based photodetection platforms. Herein, we propose a new architecture of metal-semiconductor-metal (MSM) junctions on a silicon platform to achieve efficient hot hole collection at infrared wavelengths with a photoconductance gain mechanism. The coplanar interdigitated MSM electrode's configuration forms a back-to-back Schottky diode and acts simultaneously as the plasmonic absorber/emitter, relying on the hot-spots enriched on the random Au/Si nanoholes structure. The hot hole-mediated photoelectric response was extended far beyond the cut-off wavelength of the silicon. The proposed MSM device with an interdigitated electrode design yields a very high photoconductive gain, leading to a photocurrent responsivity up to several A/W, which is found to be at least 1000 times higher than that of the existing hot carrier based photodetection strategies.

Tunable frequency matching for efficient four-wave-mixing Bragg scattering in microrings.

We propose and theoretically study a tunable frequency matching method for four-wave-mixing Bragg-scattering frequency conversion in microring resonators. A tunable coupling between the clockwise and counterclockwise propagating modes in the resonators was designed to introduce adjustable mode splitting, thus compensating for the frequency mismatching under different wavelengths. Using a silicon nitride ring resonator as an example, we showed that the tuning bandwidth approaches 35 number of FSRs. Numerical simulations further revealed that the phase-matching strategy is valid under different wavelength combinations and is robust to variations in waveguide geometry and fabrication. These results suggest promising applications in high-efficiency frequency conversion, integrated nonlinear photonics, and quantum optics.

Miniaturized spectroscopy with tunable and sensitive plasmonic structures.

A broad linewidth and a lack of spectral analysis limit the applications of plasmonic sensors. In this Letter, a plasmonic sensor with a large sensitivity in the terahertz (THz) range is proposed based on high-quality factor (>1000) surface lattice resonance in subwavelength near-flat metallic gratings. Moreover, such a highly selective spectral manipulating scheme, plus the greatly localized plasmonic resonance, enables miniaturized spectroscopy based on a single detector by integrating an electro-optical material with the gratings. A spectral resolution of 0.1 GHz at a center frequency of 1.1 THz is predicted showing a four times improvement of measuring efficiency. This technique shows promising potential in on-site matter inspection and point-of-care testing.

Reconfigurable multiphoton entangled states based on quantum photonic chips.

Multipartite entanglement is one of the most prominent features of quantum mechanics and is the key ingredient in quantum information processing. Seeking for an advantageous way to generate it is of great value. Here we propose two different schemes to prepare multiphoton entangled states on a quantum photonic chip that are both based on the theory of entanglement on the graph. The first scheme is to construct graphs for multiphoton states by the network of spatially anti-bunching two-photon sources. The second one is to construct graphs by the linear beam-splitter network, which can generate W and Dicke states efficiently with simple structure. Both schemes can be scaled up in the photon number and can be reconfigured for different types of multiphoton states. This study supplies a systematic solution for the on-chip generation of multiphoton entangled states and will promote the practical development of multiphoton quantum technologies.

The functional and mechanistic relatedness of EZH2 and menin in hepatocellular carcinoma.

BACKGROUND & AIMS: The alterations of histone modification may serve as a promising diagnostic biomarker of hepatocellular carcinoma (HCC), but the clinical and mechanistic relatedness of the histone H3 lysine 27 and 4 trimethylation (H3K27me3 and H3K4me3) in HCC remains poorly understood. Here we propose that the combination of H3K27me3 and H3K4me3 is a more precise predictive/prognostic value for outcome of HCC patients. METHODS: We used chromatin immunoprecipitation (ChIP) assays and a ChIP-on-chip screen to analyse HCC. RESULTS: We found that the EZH2 occupancy coincides with the H3K27me3 at promoters and directly silences the transcription of target genes in HCC. The H3K27me3-related gene network of EZH2 contains well-established genes, such as CDKN2A, as well as previously unappreciated genes, including FOXO3, E2F1, and NOTCH2, among others. We further observed independently increasing profiles of H3K27me3 and H3K4me3 at the promoters of certain target genes in HCC specimens. Importantly, Kaplan-Meier analysis reveals that 3-year overall and tumour-free survival rates are dramatically reduced in patients that simultaneously express EZH2 and menin, compared to rates in the EZH2 or menin under expressing patients. Furthermore, an inhibitor of H3K27me3 alone, or in combination with an H3K4me3 inhibitor, effectively blocked the aggressive phenotype of HCC cells. CONCLUSIONS: Our results indicate that a combined analysis of both H3K27me3 and H3K4me3 may serve as powerful diagnostic biomarkers of HCC, and targeting both might benefit anti-HCC therapy.

On-chip ultrasensitive and rapid hydrogen sensing based on plasmon-induced hot electron-molecule interaction.

Hydrogen energy is a zero-carbon replacement for fossil fuels. However, hydrogen is highly flammable and explosive hence timely sensitive leak detection is crucial. Existing optical sensing techniques rely on complex instruments, while electrical sensing techniques usually operate at high temperatures and biasing condition. In this paper an on-chip plasmonic-catalytic hydrogen sensing concept with a concentration detection limit down to 1 ppm is presented that is based on a metal-insulator-semiconductor (MIS) nanojunction operating at room temperature and zero bias. The sensing signal of the device was enhanced by three orders of magnitude at a one-order of magnitude higher response speed compared to alternative non-plasmonic devices. The excellent performance is attributed to the hydrogen induced interfacial dipole charge layer and the associated plasmonic hot electron modulated photoelectric response. Excellent agreements were achieved between experiment and theoretical calculations based on a quantum tunneling model. Such an on-chip combination of plasmonic optics, photoelectric detection and photocatalysis offers promising strategies for next-generation optical gas sensors that require high sensitivity, low time delay, low cost, high portability and flexibility.

Current Status of the Health Information Technology Industry in China from the China Hospital Information Network Conference: Cross-sectional Study of Participating Companies.

BACKGROUND: The China Hospital Information Network Conference (CHINC) is one of the most influential academic and technical exchange activities in medical informatics and medical informatization in China. It collects frontier ideas in medical information and has an important reference value for the analysis of China's medical information industry development. OBJECTIVE: This study summarizes the current situation and future development of China's medical information industry and provides a future reference for China and abroad in the future by analyzing the characteristics of CHINC exhibitors in 2021. METHODS: The list of enterprises and participating keywords were obtained from the official website of CHINC. Basic characteristics of the enterprises, industrial fields, applied technologies, company concepts, and other information were collected from the TianYanCha website and the VBDATA company library. Descriptive analysis was used to analyze the collected data, and we summarized the future development directions. RESULTS: A total of 205 enterprises officially participated in the exhibition. Most of the enterprises were newly founded, of which 61.9% (127/205) were founded in the past 10 years. The majority of these enterprises were from first-tier cities, and 79.02% (162/205) were from Beijing, Zhejiang, Guangdong, Shanghai, and Jiangsu Provinces. The median registered capital is 16.67 million RMB (about US $2.61 million), and there are 35 (72.2%) enterprises with a registered capital of more than 100 million RMB (about US $15.68 million), 17 (8.3%) of which are already listed. A total of 126 enterprises were found in the VBDATA company library, of which 39 (30.9%) are information technology vendors and 57 (45.2%) are application technology vendors. In addition, 16 of the 57 (28%) use artificial intelligence technology. Smart medicine and internet hospitals were the focus of the enterprises participating in this conference. CONCLUSIONS: China's tertiary hospital informatization has basically completed the construction of the primary stage. The average grade of hospital electronic medical records exceeds grade 3, and 78.13% of the provinces have reached grade 3 or above. The characteristics are as follows: On the one hand, China's medical information industry is focusing on the construction of smart hospitals, including intelligent systems supporting doctors' scientific research, diagnosis-related group intelligent operation systems, and office automation systems supporting hospital management, single-disease clinical decision support systems assisting doctors' clinical care, and intelligent internet of things for logistics. On the other hand, the construction of a compact county medical community is becoming a new focus of enterprises under the guidance of practical needs and national policies to improve the quality of grassroots health services. In addition, whole-course management and digital therapy will also become a new hotspot in the future.

•OH Inactivation of Cyanobacterial Blooms and Degradation of Toxins in Drinking Water Treatment System.

Cyanobacterial blooms continue to serve as one of the most serious global issues threatening water supply and human health. During cyanobacterial bloom season, a large •OH-yield equipment was developed and installed after coagulation settling in a 12000 ton/day drinking water treatment system in Xiamen, China. An •OH concentration of 7.76∼57.8 µmol/L was formed by using the oxygen activated species generated by strong ionisation discharge combining with the effect of water jet cavitation. •OH pre-treatment at a dose of 1.0 mg/L inactivated cyanobacterial blooms in the process of conveying bloom water within only 20s, which were then removed by sand filtration. Under SEM observation, dominant Microcystis sp. colonies connected by mucilage were dispersed into individuals that still retained the cell integrity, indicating no release of intracellular organic matter (IOM). According to a flow cytometry analysis, the main cause of •OH inactivation was the breakage of DNA strands. Meanwhile, the •OH-mineralized microcystin-LR was by breaking the C=C conjugated diene bond and crucial opening the persistent benzene ring to carboxylic acid m/z 158.0. During •OH pre-treatment of 1.0 mg/L and NaClO disinfection of 0.5 mg/L, all water quality indexes and disinfection by-product (DBP) contents complied with the Chinese Sanitary Standards for Drinking Water. Therefore, the •OH based on the strong ionisation discharge showed great prospect for large-scale drinking water treatment in the removal of cyanobacterial blooms while retaining cell integrity as well as the degradation of toxins.

Cinnamaldehyde causes apoptosis of myeloid-derived suppressor cells through the activation of TLR4.

Malignant tumors are among the most life-threatening diseases in the world. Although many different types of antitumor agents are available, severe side effects and toxicity limit their applications. Myeloid-derived suppressor cells (MDSCs) inhibit the antitumor immune response by suppressing the proliferation of T cells, the production of cytokines and the killing of tumor cells. As MDSCs have become novel targets in cancer therapy, this research focused on the anti-MDSC function of cinnamaldehyde (CA), which is extracted from cinnamon, a traditional Chinese spice. In the present study, MDSCs isolated from the spleens of mice with colon cancer were used as an in vitro model to assess the efficacy of CA. Treatment of MDSCs with CA significantly decreased cell proliferation and induced apoptotic cell death. Subsequent experiments demonstrated that CA treatment enhanced the expression of Bax and caspase-9 and inhibited the expression of Bcl-2, suggesting that CA induced apoptosis in the MDSCs via the intrinsic pathway. Taken together, the results demonstrated that CA exhibited significant anti-MDSC activity and attenuated the suppression of the antitumor immune response, indicating a potential use for CA in cancer therapy.

MPSSS impairs the immunosuppressive function of cancer-associated fibroblasts via the TLR4-NF-κB pathway.

The polysaccharides MPSSS was extracted from Lentinus edodes and has been reported to effectively inhibit tumor growth and eliminate the function of myeloid-derived immune suppressor cell-mediated T cell inhibition, thus improving the efficacy of cancer therapy. The exploration of how MPSSS affects the functions of cancer-associated fibroblasts (CAFs) will provide a new perspective for understanding the antitumor effects of MPSSS. In the present study, prostate CAFs were selected as target cells to study whether MPSSS affected cell proliferation and function. The results showed that MPSSS did not directly inhibit the growth of prostate CAFs but interfered with CAF-mediated T cell inhibition and affected the immunosuppressive function of prostate CAFs. Mechanistic studies were further performed and showed that MPSSS activated key node proteins in the NF-κB pathway that were dependent on MyD88, and a TLR4 inhibitor blocked the changes in these proteins and the effect of MPSSS. We hypothesize that MPSSS can activate the MyD88-dependent TLR4-NF-κB signaling pathway to change the function of CAFs. In conclusion, these results demonstrate that MPSSS can not only effectively inhibit the growth of prostate cancer as we previously reported but also alter the function of prostate CAFs by activating the TLR4-NF-κB pathway, providing a new strategy for the comprehensive treatment of tumors.

Ligustilide inhibits the activation of cancer-associated fibroblasts.

The purpose of this work was to study the effects and underlying molecular mechanisms of ligustilide on cancer-associated fibroblasts (CAFs). The effects of ligustilide on the growth of CAFs and splenocytes were detected by MTT assay, and flow cytometry was used to detect effects on T-cell proliferation. Western blotting was used to detect the expression levels of CAF-related proteins after ligustilide treatment. This study found that ligustilide had no effect on the growth of splenocytes but that it could change the immunosuppressive function of CAFs through the TLR4-NF-κB pathway and restore T-cell proliferation previously inhibited by the CAF supernatant. Thus, ligustilide is expected to be a candidate for new antitumor drugs.

Application of a hydroxyl-radical-based disinfection system for ballast water.

A hydroxyl radical (OH) ballast water treatment system (BWTS) was developed and applied to inactivate entrained organisms in a 10,000-ton oceanic ship, where OH was produced by a strong ionization discharge combined with a water jet cavitation effect. The calculated OH generation rate was 1373.4 µM min-1 in ballast water, which is much higher than that in other advanced oxidative processes such as photocatalysis. As a result, non-indigenous red tide algae were inactivated to meet the ballast water discharge standards (<10 cells mL-1) of the International Maritime Organization. The ratio of variable fluorescence to maximum fluorescence (Fv/Fm) for algal chlorophyll rapidly decreased to zero within a contact time of only 6 s, indicating complete inactivation of algae. Observation under a scanning electron microscope showed no cellular materials were released by algal cells upon OH inactivation. A risk assessment of the OH treatment system was conducted, and the ratios of predicted environmental concentrations to predicted no effect concentrations of all detected disinfection byproducts were less than 1, even at a worst-case oxidant concentration of 2.41 mg L-1. Ship ballast water treated using OH inactivation is safe for marine environments. Finally, the energy consumption and operational costs of the OH BWTS were found to be 0.033 kWh m-3 and CNY 0.03 m-3, respectively.

Inactivation of invasive marine species in the process of conveying ballast water using OH based on a strong ionization discharge.

In this paper, invasive marine species in medium-salinity ballast water were inactivated using OH generated from a strong ionization discharge. The OH is determined by the concentration of oxygen active species combined with the effects of water jet cavitation. The results indicated that the OH concentration reached 7.62 µM, within 1 s, which is faster and higher than in conventional AOP methods. In a pilot-scale OH ballast water system with a capacity of 10 m(3)/h, algae were inactivated when CT value was 0.1 mg min/L with a contact time only 6 s. The viable and nonviable cells were determined using SYTOX Green nucleic acid stain and Flow cytometry. As a result, the OH treatment could be completed during the process of conveying the ballast water. In addition, the concentrations of relevant disinfection by-products (DBPs), such as trihalomethanes (THMs), haloacetic acids (HAAs), and bromate, were less than that required by the World Health Organization's drinking water standards, which suggest that the discharged ballast water posed no risks to the oceanic environment. Nevertheless, for conventional ozonation and electrolysis methods, the ballast water should be treated only in the treated tanks during the ship's voyage and form higher level DBPs.

Genetic alteration and misexpression of Polycomb group genes in hepatocellular carcinoma.

Although the abnormal expression of Polycomb-group (PcG) proteins is closely associated with carcinogenesis and the clinicopathological features of hepatocellular carcinoma (HCC), the genetic mutation profile of PcG genes has not been well established. In this study of human HCC specimens, we firstly discovered a highly conserved mutation site, G553C, in the Polycomb Repressive Complex 2 (PRC2) gene enhancer of zeste homolog 2 (EZH2). This site also harbors a single nucleotide polymorphism (SNP), rs2302427, which plays an important antagonistic role in HCC. Kaplan-Meier survival curves showed that the tumor-free and overall survival of patients with EZH2 G553C were superior to those without the mutation. The G allele frequencies in patients and healthy subjects were 0.2% and 0.122%, respectively, with significant differences in distribution. The individuals carrying the GG and the GC genotypes at rs2302427 showed 3.083-fold and 1.827-fold higher risks of HCC, respectively, compared with individuals carrying the wild-type allele. Furthermore, Immunohistochemical staining revealed that the expression levels of CBX8 (in 53/123 samples) and BMI1 (in 60/130 samples) were markedly increased in human HCC specimens. Importantly, the overall and tumor-free survival rates were significantly reduced in the group of patients who simultaneously expressed PRC1 and PRC2. These results argue that a combination of PRC1 and PRC2 expression has a significant predictive/prognostic value for HCC patients. Taken together, our results indicate the abnormal expression and genetic mutation of PcG members are two independent events; cumulative genetic and epigenetic alterations act synergistically in liver carcinogenesis.