Rural revival: Navigating environmental engineering and technology.

The necessity for global engineering and technological solutions to address rural environmental challenges is paramount, particularly in improving rural waste treatment and infrastructure. This study presents a comprehensive quantitative analysis of 3901 SCI/SSCI and 3818 Chinese CSCD papers, spanning from 1989 to 2021, using tools like Derwent Data Analyzer and VOSviewer. Our key findings reveal a significant evolution in research focus, including a 716.67% increase in global publications from 1995 to 2008 and a 154.76% surge from 2015 to 2021, highlighting a growing research interest with technological hotspots in rural revitalization engineering and agricultural waste recycling. China and the USA are pivotal, contributing 784 and 714 publications respectively. Prominent institutions such as the Chinese Academy of Sciences play a crucial role, particularly in fecal waste treatment technology. These insights advocate for enhanced policy development and practical implementations to foster inclusive and sustainable rural environments globally.

Metagenomics insights into the performance and mechanism of soil infiltration systems on removing antibiotic resistance genes in rural sewage.

The removal of antibiotic resistance genes (ARGs) in sewage is of great concern, but advanced sewage treatment technologies are not suitable for rural areas, so the multi-layer soil infiltration system (MSL) has been developed for rural sewage treatment. However, little is known about the performance and function of MSL in the treatment of ARGs in rural sewage. Here, we optimized the matrix composition and structure of MSL and explored the efficacy and mechanism of MSL systems for ARG removal under different hydraulic conditions. The ARGs removal rate of MSL ranged from 41.51% to 99.67%, in which MSL with the middle hydraulic load, high pollution load, and continuous inflowing conditions showed the best removal performance. In addition, this system can operate stably and resist the temperature fluctuation, which showed an equivalent removal rate of ARGs in warm and cold seasons, amounting to 69.0%. The structural equation model revealed that microorganisms in sewage could significantly affect ARG removal (path coefficient = 0.91), probably owing to their interspecies competition. As for the internal system, the reduction of ARGs was mainly driven by microorganisms in the system matrix (path coefficient = 0.685), especially soil-mixture-block (SMB) microorganisms. The physicochemical factors of the matrix indirectly reduce ARGs by affecting the microorganisms that adhere to the matrices. Note that the pairwise alignment of nucleotide analysis demonstrated that the system matrix, especially biochar in the SMB, adsorbed ARGs and their hosts from the sewage, and in turn eliminated them by inhibiting the spread and colonization of hosts, thereby reducing the abundance of ARGs. Collectively, this study provides a deeper insight into the removal of ARGs from rural sewage by MSL, which can help improve sewage treatment technologies.

Spatial distribution of bacterial resistance towards antibiotics of rural sanitation system in China and its potential link with diseases incidence.

Chinese government is vigorously promoting toilet renovation in rural areas to reduce the risk of human feces exposure, which would cause infectious diseases, especially antibiotic resistance genes (ARGs) and pathogens. However, the distribution of ARGs in human feces from different regions of China remained ill-defined. It is not yet known how the survival of ARGs after toilet treatment is associated with the regional infection rates. Here, we investigated the prevalence of ARGs in human feces in rural areas of China and their potential relationship with infectious diseases for the first large-scale. The results showed that there were still high ARGs residues in human feces after rural toilet treatment, especially tetM-01 and ermB with average relative abundance as high as 1.21 × 10-1 (Eastern) and 1.56 × 10-1 (Northern), respectively. At a large regional scale, the significant differences in human feces resistomes were mainly shaped by the toilet types, TN, NH3-N, and the bacterial community. A critical finding was that toilets still cannot effectively decrease the pathogenicity risk in human feces. The significant positive relationship (P<0.05) between infectious diseases and ARGs can infer that ARGs in human feces exposure might be a critical path for enhancing the incidence of diseases, as these ARGs hinder the effectiveness of antibiotics.

The vertical migration of antibiotic-resistant genes and pathogens in soil and vegetables after the application of different fertilizers.

The prevalence of bacterial resistance caused by the application of animal manure has become an important environmental issue. Herein, the vertical migration of antibiotic resistance genes (ARGs) and pathogens in soil and vegetables after the application of different fertilizers was explored. The results showed that the application of composted manure considerably enhanced the abundance of most ARGs and pathogens, especially in surface soil and pakchoi roots. Moreover, the soil ARGs increased partially from log 1.93 to log 4.65 after the application of composted manure, and six pathogens were simultaneously detected. It was observed that the increase in soil depth decreased most ARGs and pathogens by log 1.04-2.24 and 53.98 %~85.54 %, respectively. This indicated that ARGs and pathogens still existed in the deep soil (80-100 cm). Moreover, total organic carbon had a significant influence on the pathogen distribution, whereas bacterial communities primarily drove the vertical migration of ARGs rather than environmental factors. Although most of the ARG-host associations observed in the surface soil were disappeared in deep soil as revealed by network analysis, some co-occurrence pattern still occurred in deep soil, suggesting that some ARGs might be carried to deep soil by their host bacteria. These results were novel in describing the vertical migration of ARGs in the environment after the application of different fertilizers, providing ideas for curbing their migration to crops.

Profiles of antibiotic- and heavy metal-related resistance genes in animal manure revealed using a metagenomic analysis.

Farmed animals produce excrement containing excessive amounts of toxic heavy metals as a result of consuming compound feed as well as receiving medical treatments, and the presence of these heavy metals may aggravate the risk of spreading drug-resistance genes through co-selection during manure treatment and application processes. However, research on the association between heavy metals and antimicrobial resistance is still lacking. In this study, metagenomic sequencing was used to explore the effects of the co-selection of environmentally toxic heavy metals on the resistome in manure. A relevance network analysis showed that metal-resistance genes (MRGs), especially for copper (Cu) and zinc (Zn), were positively correlated with multiple types of antibiotic-resistance genes (ARGs) and formed a complex network. Most bacteria that co-occurred with both MRGs and ARGs simultaneously are members of Proteobacteria and accounted for 54.7% of the total microbial species in the relevance network. The remaining bacteria belonged to Firmicutes, Bacteroidetes and Actinobacteria. Among the four phyla, Cu- and Zn-resistance genes had more complex correlations with ARGs than other MRG types, reflecting the occurrence of ARG co-selection under the selective pressure of high Cu and Zn levels. In addition, approximately 64.8%, 59.1% and 68.4% of MRGs that correlated with the presence of plasmids, viruses and prophages, respectively, are Cu- or Zn-resistant, and they co-occurred with various ARGs, indicating that mobile genetic elements participate in mediating ARG co-selection in response to Cu and Zn pressure. The results indicated that the use of heavy-metal additives in feed induces the increases of drug resistance genes in manure through co-selection, aggravating the risk of antimicrobial resistance diffusion from animal farm to manure land applications.

Dynamic changes of microbial community and moisture ratio during bio-drying of sludge after electro-dewatering.

Using electro-dewatering as the pretreatment process for sludge bio-drying can improve the dewatering performance. It was innovatively investigated including the microbial mechanism and the kinetics of moisture removal by bio-drying with electro-dewatered sludge in this study. Two bio-drying processes using electro-dewatered sludge (EDS) and sludge added cornstalk conditioner (CSS) were compared. Microbial community analysis showed that the abundance of Bacteroidetes increased from 4.21% to 16.67% after electro-dewatering. The dominant phyla were Bacteroidetes (36.79%), Proteobacteria (32.35%), and Actinobacteria (24.58%) at the end of EDS bio-drying. Network analysis revealed that the co-occurrence patterns in EDS included 40 nodes and 97 edges. The prediction results of the Kyoto Encyclopedia of Genes and Genomes demonstrated that the relative abundances of carbohydrate metabolism and metabolism of terpenoids and polyketides in sludge decreased, while the relative abundances of lipid metabolism, xenobiotic biodegradation and metabolism increased after electro-dewatering. Five thin layer drying kinetic models were analyzed to estimate the bio-drying kinetic parameters. The Page's model could be better fitted to the results and the highest R2 was 0.9570 in the EDS. The new coefficients k (0.1637) and n (1.2097) were obtained. The results provided mechanism and data support for exploring and applying bio-drying technology after sludge electro-dewatering.

What role does organic fertilizer actually play in the fate of antibiotic resistome and pathogenic bacteria in planting soil?

Organic fertilizer increase antibiotic resistance genes (ARGs) and bacterial pathogens have widely documented. However, how organic fertilizer is involved in changing soil ARGs and pathogenic bacteria after long-term (≥5 years) application remains unclear. Herein, the ARGs and pathogenic bacteria were compared in organic fertilized soils (AF) and non-fertilized soils (NF), and the contribution of input sources (organic fertilizer, irrigation water, air and background soil) on soil ARGs also was determined in this study. Results showed that the abundances of some ARGs, such as vanR and aac(6')-I in AF, were significantly higher than these of NF (p < 0.05). And a relatively higher abundance of potential pathogens, especially, Salmonella enterica and Stenotrophomonas maltophilia, in AF was observed. This indicated that organic fertilizer application can maintain a high level of some soil ARGs and pathogenic bacteria for at least 5 years. Traceability analysis unearthed that organic fertilizer application mainly increased its own contribution to soil ARGs from 1.16% to 9.05%, as well reduced the contribution of background soil, suggesting that the increase in soil ARGs may be partly attributable to organic fertilizer inputs. Notably, organic fertilizer application did not significantly alter the contribution ratio of input sources to microorganisms, but there was a clear change in the composition of soil microorganisms, which meant that the effect of the input source on the microorganism may emanate from other factors, rather than direct inputs. Subsequent structural equation demonstrated that organic fertilizer application significantly enhanced the effect of environmental factors on ARGs, and also indirectly increased the influence of communities on ARGs. Collectively, under the long-term fertilization, the role of organic fertilizers on soil ARGs not just stems from its own input, and also dominates the influence of environmental factors on ARGs. This study elucidates main causes for the difference in ARGs in AF vs. NF and enlightens actual role of organic fertilizer in them.

Systematically assess the advancing and limiting factors of using the multi-soil-layering system for treating rural sewage in China: From the economic, social, and environmental perspectives.

Solving the problem of rural sewage is considered an essential task in China's rural revitalization strategy. Based on the yearbook data of sewage treatment in rural areas between 2014 and 2019, although the rate of sewage treatment in rural areas of China showed an upward trend, it was still below 35%, mainly due to the lack of suitable sewage treatment technologies. Here, we discuss the multi-soil-layering (MSL) system, which is an emerging technology suitable for rural sewage treatment. It was deemed to overcome the shortcomings of current biological and ecological treatment technologies, such as complex operation, large area, and high operating costs. We used system dynamics to evaluate the advancing and limiting factors of MSL application for rural sewage treatment from the social, environmental, and economic dimensions. The results illustrated a complete causal loop diagram in which essential variables and relationships were concentrated in the technology, operation and maintenance, and satisfaction of farmers. The efficiency of MSL is the key variable affecting the final decision of the MSL application. Overall, using MSL to treat rural sewage could be an option to improve the rural environment in China. However, the scientific technological model for MSL should be further explored. This review provides guidance on how to promote MSL systems in rural areas.

Optimising mixed aerobic and anaerobic composting process parameters for reducing bacterial pathogenicity in compost-derived products.

Although composting techniques are continuously optimised and adjusted, the removal of bacterial pathogen based on the quality of composting products needs further to ensure safe of agricultural use. In this study, we combined aerobic composting and anaerobic process to determine the optimal combination (turning frequency of once a day, the proportion of swine manure to corn straw (3:1), and mixed 6-day anaerobic process) that benefits the reduction of bacterial pathogens, among which the maximum removal efficiency of up to 92.96% was observed for Clostridium_sensu_stricto_1 reached, thereby improving the quality of the compost products. The variation partition analysis and redundancy analysis indicated that physicochemical factors such as temperature, TOC, and pH significantly affected the removal of bacterial pathogens. Therefore, the additive effects of physicochemical factors on bacterial pathogen removal requires further process optimisation. These findings offer powerful technological support for improving agricultural waste recycling and enhancing the safety of fertiliser application.

Fate of antibiotic resistance genes in farmland soil applied with three different fertilizers during the growth cycle of pakchoi and after harvesting.

The increasing prevalence of antibiotic resistance genes (ARGs) in the soil environment poses a serious threat to crop safety and even public health. In this study, the fate of ARGs in the soil was investigated during the growth period of pakchoi and after harvesting with the application of different kinds of fertilizers. The result showed that increasing rate of soil ARGs during the growth period of pakchoi followed the order of composted manure > commercial fertilizer > mineral fertilizer. After harvesting, soil ARGs abundance treated with mineral fertilizer, commercial fertilizer or composted manure significantly increased by 0.63, 3.19 and 8.65 times (p < 0.05), respectively, compared with the non-fertilized soil. The ARGs abundance in the pakchoi treated with composted manure was significantly higher than that of treatments with mineral fertilizer and commercial organic fertilizer. These findings indicated the application of composted pig manure would significantly increase the pollution load of ARGs in farmland soil and plant, and also promote the proliferation of farmland ARGs. Principal component analysis suggested that bacterial communities might have a significant influence on ARGs changes during the growth period of pakchoi. Network analysis further indicated ARGs changes may be mainly related to their host bacteria (including Gammaproteobacteria, Flavobacteriia and Bacilli). The results provided a proper method and useful information on reducing transmission risk of ARGs and control the propagation of ARGs in agricultural activities.

Three-compartment septic tanks as sustainable on-site treatment facilities? Watch out for the potential dissemination of human-associated pathogens and antibiotic resistance.

Improved sanitation is critical important to reduce the spread of human deposited pathogens and antibiotic resistance genes (ARGs). In the China's rural "Toilet Revolution", three-compartment septic tanks (SPTs) are widely used as household domestic sewage treatment facilities. The effluents of SPTs are encouraged to be used as fertilizer in agriculture. However, whether SPT could eliminate fecal pathogens and ARGs is still unrevealed which is crucial in risk assessment of SPT effluent utilization. Herein, we employed metagenomic sequencing to investigate the pathogens and ARGs in rural household SPTs from Tianjin, China. We found that rural household SPT effluents conserved pathogens comparable to that of the influents. A total of 441 ARGs conferring resistance to 26 antibiotic classes were observed in rural household SPTs, with the relative abundance ranging from 709 to 1800 ppm. Results of metagenomic assembly indicated that some ARG-MGE-carrying contigs were carried by pathogens, which may pose risk to human and animal health after being introduced to the environment. This study raises the question of SPTs as sustainable on-site treatment facilities for rural domestic sewage and underscores the need for more attention to the propagation and dissemination of antibiotic-resistant pathogens from SPT to the environments, animals, and humans.

Effects of foliar dressing of selenite and silicate alone or combined with different soil ameliorants on the accumulation of As and Cd and antioxidant system in Brassica campestris.

This study was conducted to investigate the possibility of using a combined technology to synchronously reduce As and Cd accumulation in the edible parts of Brassica campestris. The results showed that a foliar application of selenite (Se) and silicon (Si) combined with soil ameliorants (including Ca-Mg-P fertilizer, sodium silicate and red mud) showed limited effects on the growth of B. campestris. The As concentration in the leaves of B. campestris in all treatments was below the Chinese safety standard. When sodium silicate and Ca-Mg-P fertilizer were added to the soil, the additional foliar application of Se and Si could in some cases help further reduce the concentrations of As and Cd in the leaves of B. campestris. However, when red mud was applied to the soil, the foliar application of Se and Si enhanced the Cd concentration in the leaves of B. campestris. In most cases, high levels of soil ameliorants plus foliar application of Se and Si significantly enhanced the As concentrations in both the soil solution and the roots of B. campestris but reduced the soil solution Cd concentration and the leaf As concentration. Most of the treatments reduced the thiobarbituric acid reactive substances (TBARS) concentration in the leaves of B. campestris, and the foliar application of Se and Si helped the soil ameliorants alleviate the oxidative stress resulting from As and Cd exposure. In this study, several treatments significantly increased the activities of superoxide dismutase (SOD) and ascorbate peroxidase (APX). However, the enzymes peroxidase (POD) and catalase (CAT) were not induced by most treatments. In summary, the combined treatment of 1gkg-1 Ca-Mg-P fertilizer plus foliar spraying 2mmolL-1 sodium selenite was most effective in reducing the Cd concentration and a rather strong ability to reduce the As concentration and trigger the activities of SOD and APX in the leaves of B. campestris.

Evaluation of silkworm excrement and mushroom dreg for the remediation of multiple heavy metal/metalloid contaminated soil using pakchoi.

The economical, environmental friendly and efficient materials to remediate the pollution with multiple heavy metals and metalloids are scarce. Silkworm excrement (SE) and mushroom dregs (MD) are two types of agricultural wastes, and they are widely used to improve the soil fertility in many regions of China. A pot experiment with sixteen treatments was set up to assess the possibility of using SE and MD to stabilize heavy metals and metalloids and reduce their uptake in pakchoi cultivated in slightly contaminated soils with arsenic (As), cadmium (Cd), lead (Pb) and zinc (Zn). The results showed that the single addition of SE obviously stimulated the growth of pakchoi, reduced the contents of all tested heavy metals and metalloids in the edible part of pakchoi and availability of Zn and Cd in soil. The single MD treatment showed an inferior ability to enhance the growth and reduce the contents of heavy metals and metalloids in the edible part of pakchoi. The combined utilization of SE and MD appeared not to show better effects than their individual treatment when using them to remediate this contaminated soil. Some potential mechanisms on the stimulation on pakchoi growth and decreasing the accumulation of heavy metals and metalloids in pakchoi subjected to SE were suggested, including: (1) enhancing soil pH to impact the availability of heavy metals and metalloids; (2) improve the fertility of soil; (3) sulfhydryl groups of organic materials in SE play a role in conjugating heavy metals and metalloids to affect their availability in soil; and (4) stimulating the growth of pakchoi so as to show a "dilution effect" of heavy metals and metalloids.

Lanthanum modified chitosan-attapulgite composite for phosphate removal from water: Performance, mechanisms and applicability.

A novel lanthanum-modified-chitosan-attapulgite (La-CTS-ATP) composite with efficient adsorption of phosphate in water was prepared and characterized. The adsorption properties were investigated by adsorption kinetics, adsorption isotherms and thermodynamics. An orthogonal design test was used to explore the optimal preparation conditions of La-CTS-ATP. Various characterizations exhibited the successful modification of lanthanum and the effective adsorption of phosphate. The batch experiments showed that La-CTS-ATP exhibited excellent stability within pH range of 4-10 and the disturbance of co-existing substances (Cl-, NO3-, HCO3-, SO42-). Chemisorption played the main role in phosphate adsorption from kinetic studies. Isotherm studies illustrated monolayer uptake of adsorbents onto the adsorbent, and the maximum adsorption capacity was 102.90 mg/g, much higher than other La-based adsorbents. The reaction process was spontaneous (ΔG < 0), exothermic (ΔH < 0) and disordered (ΔS > 0) from thermodynamic analysis. The excellent reusability of La-CTS-ATP was investigated, after five cycles, the phosphate removal rate and desorption rates were 72.93 % and 77.44 %, respectively. The dynamic adsorption experiments showed that the faster the flow rate, the shorter the time for La-CTS-ATP to get saturation, and the maximum adsorption capacity was slightly reduced. The surface precipitation, electrostatic attraction, and ligand exchange were the main mechanisms affecting its adsorption behavior. The adsorbent exhibited excellent effectiveness in real wastewater (breeding wastewater and domestic wastewater). In this study, a novel and effective adsorbent for phosphorus removal was produced in contaminated water, and the analysis of the adsorption process of the system provides new insights for related research.

Hydrothermal Ammonia Carbonization of Rice Straw for Hydrochar to Separate Cd(II) and Zn(II) Ions from Aqueous Solution.

Hydrochar is considered to be a good adsorbent for the separation of metal ions from aqueous solutions. However, the yield of hydrochar from raw straw is generally low, because the hydrothermal carbonization occurs via dehydration, polymerization, and carbonization. In this work, various hydrochar samples were prepared from rice straw with nitrogen and phosphorus salt; moreover, toilet sewage was used instead of nitrogen, and phosphorus salt and water were used to promote the polymerization and carbonization process. The modified carbon was characterized using XRD, XPS, SEM, and FTIR, and the adsorption capacity was investigated. A significant increase in hydrochar yield was observed when toilet sewage was used as the solvent in the hydrothermal carbonization process. The adsorption capacity of N/P-doped rice straw hydrochar for Cd2+ and Zn2+ metal ions was 1.1-1.4 times higher than that those using the rice straw hydrochar. The Langmuir models and pseudo-second-order models described the metal adsorption processes in both the single and binary-metal systems well. The characterization results showed the contribution of the surface complexation, the electrostatic interaction, the hydrogen bond, and the ion exchange to the extraction of Cd2+ and Zn2+ using N/P-doped rice straw hydrochar.

Effects of Mercury Contamination on Microbial Diversity of Different Kinds of Soil.

Soil microorganisms promote the recovery of contaminated soil by influencing the cyclic transformation of various substances. In this study, we investigated the impact of mercury pollution on the structure, composition, and main populations of soil microbial communities using a high-throughput sequencing method and observed that mercury pollution significantly influenced the diversity, structure, and distribution pattern of microbial communities. Furthermore, during mercury pollution, the Shannon and Chao indices decreased for the bacterial communities and increased for the fungal communities. Mercury pollution mainly reduced the relative abundances of Proteobacteria (16.2−30.6%), Actinomycetes (24.7−40.8%), and other dominant bacterial phyla. The relative abundance of Ascomycota decreased by 17.4% and 16.7% in alkaline and neutral soils, respectively, whereas the relative abundance of unclassified_k_Fungi increased by 26.1% and 28.6%, respectively. In acidic soil, Ascomycota increased by 106.3% and unclassified_k_Fungi decreased by 71.2%. The results of redundancy and correlation analyses suggested that soil microbial diversity was significantly correlated with soil properties such as pH, cation exchange capacity, soil organic carbon, and total nitrogen (p < 0.05) under different treatments. Our findings highlight the impact of Hg pollution on soil microbial communities, thereby providing a theoretical foundation for the bioremediation of soil Hg pollution.

Mutagenesis of high-efficiency heterotrophic nitrifying-aerobic denitrifying bacterium Rhodococcus sp. strain CPZ 24.

Breeding high-efficiency heterotrophic nitrifying-aerobic denitrifying (SND) bacteria is important for the removal of biological nitrogen in wastewater treatment. In this study, a high-efficiency SND mutant strain, ΔRhodococcus sp. CPZ 24, was obtained by ultraviolet-diethyl sulfate compound mutagenesis. The maximum nitrification and denitrification rates were 3.77 and 1.37 mg·L-1·h-1, respectively 30.30 % and 17.10 % higher than those of wild bacteria. Biolog technology and network model analysis revealed that ΔCPZ 24 significantly improved the utilisation ability and metabolic activity of organic carbon sources. Furthermore, the expression levels of the nitrogen removal function genes nxrA, nosZ, amoA, and norB in strain ΔCPZ 24 increased significantly. In actual sewage, mutant bacteria ΔCPZ 24 have a 95.05 % ammonia-nitrogen degradation rate and a 96.67 % nitrate-nitrogen degradation rate. These results suggested that UV-DES compound mutation was a successful strategy to improve the nitrogen removal performance of SND bacteria in wastewater treatment.

Coconut shell and its biochar as fertilizer amendment applied with organic fertilizer: Efficacy and course of actions on eliminating antibiotic resistance genes in agricultural soil.

Biomass amendments have numerous benefits in reducing antibiotic resistance genes (ARGs) in the soil environment. However, there are debatable outcomes regarding the effect of raw biomass and its pyrolytic biochar on ARGs, and the exploration of the influence mechanism is still in infancy. Herein, we investigated the changes in soil ARGs under the organic fertilizer application with coconut shell and its biochar. The results showed that the coconut shell biochar could effectively diminish ARGs, with 61.54% reduction in target ARGs, which was higher than that adding raw coconut shells (p < 0.05). Structural equation modeling indicated that ARGs were significantly affected by changes in environmental factors, mainly by modulating bacterial communities. Neutral community model and network analysis demonstrated that the coconut shell biochar can restrict the species dispersal, thereby mitigating the spread of ARGs. Also, coconut shell biochar exhibited strong adsorption, with a large specific surface area (476.66 m2/g) and pores (pore diameter approximately 1.207 nm, total pore volume: 0.2451 m3/g), which markedly enhanced soil heterogeneity that created a barrier to limit the resistant bacteria proliferation and ARGs propagation. The outcome gives an approach to control the development of ARGs after organic fertilizer application into soil.

Full-Scale of a Compost Process Using Swine Manure, Human Feces, and Rice Straw as Feedstock.

Regarding the composting of rural waste, numerous studies either addressed the composting of a single waste component or were conducted at a laboratory/pilot scale. However, far less is known about the mixed composting effect of multi-component rural waste on a large scale. Here, we examined nutrient transformation, maturity degree of decomposition, and succession of microbial communities in large-scale (1,000 kg mixed waste) compost of multi-component wastes previously optimized by response models. The results showed that multi-component compost can achieve the requirement of maturity and exhibit a higher nutritional value in actual compost. It is worth noting that the mixed compost effectively removed pathogenic fungi, in which almost no pathogenic fungi were detected, and only two pathogenic bacteria regrown in the cooling and maturation stages. Structural equation models revealed that the maturity (germination index and the ratio of ammonium to nitrate) of the product was directly influenced by compost properties (electrical conductivity, pH, total organic carbon, moisture, temperature, and total nitrogen) compared with enzymes (cellulase, urease, and polyphenol oxidase) and microbial communities. Moreover, higher contents of total phosphorus, nitrate-nitrogen, and total potassium were conducive to improving compost maturity, whereas relatively lower values of moisture and pH were more advantageous. In addition, compost properties manifested a remarkable indirect effect on maturity by affecting the fungal community (Penicillium and Mycothermus). Collectively, this evidence implies that mixed compost of multi-component rural waste is feasible, and its efficacy can be applied in practical applications. This study provides a solution for the comprehensive treatment and utilization of rural waste.

Excessive application of chemical fertilizer and organophosphorus pesticides induced total phosphorus loss from planting causing surface water eutrophication.

Total phosphorus (TP) loss from planting was one of the resources causing agricultural non-point source pollution. It is significant to clarify the factors influencing TP loss, as well as explore the relationship between TP loss from planting and surface water eutrophication for making recommendations on the reduction of environmental pollution. In this study, the minimum and maximum of average TP loss was appeared in Qinghai and Shandong province with the TP loss of 7.7 × 102 t and 7.5 × 103 t from 2012 to 2014, respectively. The results of structural equation model (SEM) indicating that the effect of anthropogenic drivers on TP loss was more important than natural conditions due to the higher path coefficient of anthropogenic drivers (0.814) than that of natural conditions (0.130). For anthropogenic drivers, the path coefficients of usage of fertilizer and pesticides, which was often excessively applied in China, were 0.921 and 0.909, respectively causing they the two dominant factors affecting TP loss. Annual precipitation and relative humidity, which were belongs to natural conditions, increased TP loss by enhancing leaching and surface runoff. However, light duration could reduce TP loss by promoting crop growth and increasing TP absorption of crops, with a path coefficient of - 0.920. TP loss of each province in per unit area from planting was significantly correlated with TP concentration of its surface water (p < 0.05), suggesting that TP loss from planting was the main factor causing surface water eutrophication. This study targeted presented three proposals to reduce the TP loss from planting, including promotion of scientific fertilization technologies, restriction of organophosphorus pesticides, and popularization of water saving irrigation technologies. These findings as well as suggestions herein would provide direction for the reduction of TP loss from planting.