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1.
J Am Chem Soc ; 146(15): 10573-10580, 2024 Apr 17.
Artículo en Inglés | MEDLINE | ID: mdl-38567542

RESUMEN

Atomic thick two-dimensional (2D) materials with exciting physical, chemical, and electronic properties are gaining increasing attention in next-generation science and technology, showing great promise in catalysis and energy science. However, the precise design and synthesis of efficient catalytic systems based on such materials still face many difficulties, especially in how to control the preparation of structurally determined, highly active, atomic-scale distribution of material systems. Here, we report that a highly active zerovalent osmium single-atom-layer with a thickness of single atom size has been successfully and controllably self-organized on the surface of 2D graphdiyne (GDY) material. Detailed characterizations showed that the incomplete charge transfer effect between the Os atoms and GDY not only stabilized the catalytic system but also improved the intrinsic activity, making the Gibbs free energy reach the best and resulting in remarkable performance with a small overpotential of 49 mV at 500 mA cm-2, large specific j0 of 18.6 mA cm-2, and turnover frequency of 3.89 H2 s-1 at 50 mV. In addition, the formation of sp-C-Os bonds guarantees the high long-term stability of 800 h at a large current density of 500 mA cm-2 in alkaline simulated seawater.

2.
Angew Chem Int Ed Engl ; 63(9): e202316723, 2024 Feb 26.
Artículo en Inglés | MEDLINE | ID: mdl-38192242

RESUMEN

The manufacture of nitric acid (HNO3 ) consumes large amounts of energy and causes serious environmental pollution. Electrochemical synthesis is regarded as a key way to eliminate carbon emissions from the chemicals industry. The selective electrosynthesis of HNO3 from nitrogen was achieved by controllable assembly of cobalt metal on graphdiyne surface using a powerful tool of electrochemistry at ambient conditions. As an advanced material, graphdiyne (GDY) has a large conjugated structure on its surface and is rich in sp-C triple bond skeleton, which can achieve strong interaction with metal atoms, resulting in incomplete charge transfer between graphdiyne and cobalt atoms. The experimental and theoretical calculation results show that the highly oxidized cobalt on graphdiyne (HOCo/GDY) can selectively and efficiently activate and convert the nitrogen into the key intermediate *NO, which promotes the efficient overall conversion performance of nitrogen to nitric acid. Thus, the highest nitric acid yield (192.0 µg h-1 mg-1 ) and Faradaic efficiency (21.5 %) were achieved at low potentials.

3.
Angew Chem Int Ed Engl ; : e202406043, 2024 Jun 12.
Artículo en Inglés | MEDLINE | ID: mdl-38866704

RESUMEN

Metal atom catalysts have been among the most important research objects due to their specific physical and chemical properties. However, precise control of the anchoring of metal atoms is still challenging to achieve. Cobalt and iridium atomic arrays formed sequentially ordered stable arrays in graphdiyne (GDY) triangular cavities depending on their intrinsic chemical properties and interactions. The success of this method was attributed to multifunctional integration of GDY, enabling selective growth from one to several atoms and various atomic densities. The bimetallic atom arrays show several advantages resulting from reducibility of acetylene bonds, space limiting effect, incomplete charge transfer between GDY and metal atoms, and sp-C hybridized triple bond skeleton. This well-designed system exhibits unprecedented oxygen evolution reaction (OER) performance with a mass activity of 2.6 A mgcat.-1 at a low overpotential of 300 mV, which is 216.6 times higher than the state-of-the-art IrO2 catalyst, and long-term stability.

4.
Angew Chem Int Ed Engl ; 62(41): e202310722, 2023 Oct 09.
Artículo en Inglés | MEDLINE | ID: mdl-37642147

RESUMEN

Selective hydrogenation of olefins with water as the hydrogen source at ambient conditions is still a big challenge in the field of catalysis. Herein, the electrocatalytic hydrogenation of purely aliphatic and functionalized olefins was achieved by using graphdiyne based copper oxide quantum dots (Cux O/GDY) as cathodic electrodes and water as the hydrogen source, with high activity and selectivity in aqueous solution at high current density under ambient temperature and pressure. In particular, the sp-/sp2 -hybridized graphdiyne catalyst allows the selective hydrogenation of cis-trans isomeric olefins. The chemical and electronic structure of the GDY results in the incomplete charge transfer between GDY and Cu atoms to optimize the adsorption/desorption of the reaction intermediates and results in high reaction selectivity and activity for hydrogenation reactions.

5.
J Am Chem Soc ; 144(4): 1921-1928, 2022 Feb 02.
Artículo en Inglés | MEDLINE | ID: mdl-35044172

RESUMEN

The development of efficient and durable electrocatalysts is the only way to achieve commercial fuel cells. A new, efficient method was utilized for epitaxial growth of gold quantum dots using atomically platinum chlorine species with porous graphdiyne as a support (PtCl2Au(111)/GDY), for obtaining successful multicomponent quantum dots with a size of 2.37 nm. The electrocatalyst showed a high mass activity of 175.64 A mgPt-1 for methanol oxidation reactions (MORs) and 165.35 A mgPt-1 for ethanol oxidation reactions (EORs). The data for this experiment are 85.67 and 246.80 times higher than those of commercial Pt/C, respectively. The catalyst also showed highly robust stability for MORs with negligible specific activity decay after 110 h at 10 mA cm-2. Both structure characterizations and theoretical calculations reveal that the excellent catalytic performance can be ascribed to the chlorine introduced to modify the d-band structure on the Pt surface and suppression of the CO poisoning pathway of the MOR. Our results indicate that an atomically dispersed metal species tailoring strategy opens up a new path for the efficient design of highly active and stable catalysts.

6.
J Virol ; 92(22)2018 11 15.
Artículo en Inglés | MEDLINE | ID: mdl-30158291

RESUMEN

The Japanese encephalitis virus (JEV) envelope (E) protein, as one of mediators of virus entry into host cells, plays a critical role in determining virulence. The Glu-to-Lys mutation of residue 138 in E protein (E138) plays an important role in attenuating JEV vaccine strain SA14-14-2. However, it is not clear how E138 attenuates JEV. Here, we demonstrate that the Glu-to-Arg mutation of E138 also determines the attenuation of JEV strain 10S3. Likewise, for its parent strain (HEN0701), a virulence strain, the mutations of E138 are responsible for virulence alteration. Furthermore, we demonstrated that mutations of alkaline residues in E138 contributed to the attenuation of neurovirulence; in contrast, mutations of acidic residues enhanced the neurovirulence of the strains. Moreover, acidity in residue E47 had a similar effect on neurovirulence. Furthermore, the alkaline E138 residue enhanced susceptibility to heparin inhibition in vitro and limited JEV diffusion in mouse brain. These results suggest that the acidity/alkalinity of the E138 residue plays an important role in neurovirulence determination.IMPORTANCE The E protein is the only glycoprotein in mature JEV, and it plays an important role in viral neurovirulence. E protein mutations attenuate JEV neurovirulence through unclear mechanisms. Here, we discovered that E138 is a predominant determinant of JEV neurovirulence. We demonstrated that the alkalinity/acidity of E138 determines JEV neurovirulence. These data contribute to the characterization of the E protein and the rational development of novel JEV vaccines.


Asunto(s)
Virus de la Encefalitis Japonesa (Especie)/genética , Virus de la Encefalitis Japonesa (Especie)/patogenicidad , Proteínas del Envoltorio Viral/genética , Secuencia de Aminoácidos , Sustitución de Aminoácidos/genética , Animales , Línea Celular , Cricetinae , Virus de la Encefalitis Japonesa (Especie)/clasificación , Encefalitis Japonesa/virología , Glicoproteínas/genética , Humanos , Concentración de Iones de Hidrógeno , Ratones , Mutación/genética , Virulencia/genética
7.
Virus Genes ; 53(1): 4-14, 2017 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-27665292

RESUMEN

Flavivirus cDNA clones frequently demonstrate genetic instability in transformed bacteria, which hampers the construction and manipulation of cDNAs for infectious flaviviruses. In this study, we developed a stable, full-length cDNA clone, pJEHEN, of a GI JEV strain HEN0701 using a medium-copy-number pBR322 vector and propagating cDNA clones at room temperature. The virus vJEHEN recovered from the infectious clone was indistinguishable from the parent virus HEN0701 with respect to plaque morphology, growth kinetics, and virulence characteristics. A T-to-A silent mutation of nucleotide 24 of the NS2a gene was introduced into the infectious cDNA clone to eliminate frameshifting. The rescued mutant virus vJETA did not express NS1' in infected cells and showed reduced growth and neurovirulence in mice. This convenient method for the construction and manipulation of infectious JEV cDNA clones may be of use in further studies to improve our understanding of the molecular mechanisms responsible for JEV replication and pathogenesis.


Asunto(s)
ADN Complementario , Virus de la Encefalitis Japonesa (Especie)/genética , Genoma Viral , ARN Viral , Animales , Línea Celular , Modelos Animales de Enfermedad , Encefalitis Japonesa/mortalidad , Encefalitis Japonesa/virología , Orden Génico , Ratones , Mutación , Transfección , Virulencia , Replicación Viral
8.
Virus Genes ; 52(2): 195-203, 2016 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-26888374

RESUMEN

The genetic instability of Flavivirus cDNA clones in transformed bacteria is a common phenomenon. Herein, a cDNA fragment of the nucleotide (nt) 1-2913 of the genome of a flavivirus, Japanese encephalitis virus (JEV), was used to investigate factors that caused the instability of cDNA clones. Several cDNA fragments with different 5'- or 3'-termini of the 2913-nt cDNA were obtained by PCR amplification or restriction enzyme digestion and cloned into a pCR-Blunt II-TOPO vector. All the cDNA fragments were stably propagated at 25 °C. However, the 5'-untranslated region and half of the 3'-E gene could cause the instability of the 2913-nt cDNA at 37 °C. The 5'-terminus sequences of the 2913-nt fragment were subjected to testing of the prokaryotic promoter activity by luciferase assay and Western blot. The sequences of 54-120 nt of the JEV genome exhibited high prokaryotic promoter activity at 37 °C, and the activity declined markedly at 25 °C. These findings revealed that the high prokaryotic promoter activity of the 54-120 nt sequences of the JEV genome together with expression of JEV structural genes determined the instability of a JEV cDNA clone. Growth at room temperature may reduce the prokaryotic promoter activity of 5'-sequences of the JEV genome and could represent an effective way to improve the stability of flavivirus cDNA clones in host bacteria.


Asunto(s)
ADN Complementario , Virus de la Encefalitis Japonesa (Especie)/genética , Escherichia coli/genética , Inestabilidad Genómica , ARN Viral/genética , Regiones no Traducidas 5' , Codón Iniciador , Expresión Génica , Genes Reporteros , Mutagénesis Sitio-Dirigida , Sistemas de Lectura Abierta , Regiones Promotoras Genéticas
9.
Arch Virol ; 159(9): 2513-7, 2014 Sep.
Artículo en Inglés | MEDLINE | ID: mdl-24719194

RESUMEN

The SD0803 strain of the bovine viral diarrhea virus (BVDV) was isolated from a piglet in China in 2008 and has been classified as a novel subgenotype of BVDV-1. To describe the molecular features of this novel subgenotype, we sequenced and characterized the complete genome of the SD0803 virus. The genome is 12,271 bp in length and contains 5' and 3' untranslated regions (UTRs) that flank an open reading frame (ORF) encoding a 3,898-amino-acid polypeptide. The full-length genome of the SD0803 strain shares 78.8% to 83.3% identity with those of other BVDV-1 strains, 70.0% to 70.7% identity with those of BVDV-2 strains, and less than 67.6% identity with those of other pestiviruses. The highest level of shared identity was 83.3% between the complete SD0803 genome and that of the ZM-95 strain of BVDV-1. Phylogenetic analysis of the 5' UTR and the coding sequence for the N-terminal protease fragment of the SD0803 polyprotein indicated that the SD0803 virus is a member of the novel subgenotype BVDV-1q, isolates of which have been identified recently in dairy cattle and camels in China.


Asunto(s)
Virus de la Diarrea Viral Bovina Tipo 1/clasificación , Virus de la Diarrea Viral Bovina Tipo 1/genética , Genoma Viral , Infecciones por Pestivirus/veterinaria , ARN Viral/genética , Análisis de Secuencia de ADN , Porcinos/virología , Regiones no Traducidas 3' , Regiones no Traducidas 5' , Animales , China , Análisis por Conglomerados , Virus de la Diarrea Viral Bovina Tipo 1/aislamiento & purificación , Datos de Secuencia Molecular , Sistemas de Lectura Abierta , Infecciones por Pestivirus/virología , Filogenia , Homología de Secuencia
10.
Natl Sci Rev ; 11(8): nwae189, 2024 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-39007000

RESUMEN

A major impediment to the development of the efficient use of artificial photosynthesis is the lack of highly selective and efficient photocatalysts toward the conversion of CO2 by sunlight energy at room temperature and ambient pressure. After many years of hard work, we finally completed the synthesis of graphdiyne-based palladium quantum dot catalysts containing high-density metal atom steps for selective artificial photosynthesis. The well-designed interface structure of the catalyst is composed of electron-donor and acceptor groups, resulting in the obvious incomplete charge-transfer phenomenon between graphdiyne and plasmonic metal nanostructures on the interface. These intrinsic characteristics are the origin of the high performance of the catalyst. Studies on its mechanism reveal that the synergism between 'hot electron' from local surface plasmon resonance and rapid photogenerated carrier separation at the ohmic contact interface accelerates the multi-electron reaction kinetics. The catalyst can selectively synthesize CH4 directly from CO2 and H2O with selectivity of near 100% at room temperature and pressure, and exhibits transformative performance, with an average CH4 yield of 26.2 µmol g-1 h-1 and remarkable long-term stability.

11.
Natl Sci Rev ; 10(2): nwac209, 2023 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-36817842

RESUMEN

A major impediment to industrial urea synthesis is the lack of catalysts with high selectivity and activity, which inhibits the efficient industrial production of urea. Here, we report a new catalyst system suitable for the highly selective synthesis of industrial urea by in situ growth of graphdiyne on the surface of cobalt-nickel mixed oxides. Such a catalyst is a multi-heterojunction interfacial structure resulting in the obvious incomplete charge-transfer phenomenon between a graphdiyne and metal oxide interface and multiple intermolecular interactions. These intrinsic characteristics are the origin of the high performance of the catalyst. Studies on the mechanism reveal that the catalyst could effectively optimize the adsorption/desorption capacities of the intermediate and promote direct C-N coupling by significantly suppressing by-product reactions toward the formation of H2, CO, N2 and NH3. The catalyst can selectively synthesize urea directly from nitrite and carbon dioxide in water at room temperature and pressure, and exhibits a record-high Faradaic efficiency of 64.3%, nitrogen selectivity (Nurea-selectivity) of 86.0%, carbon selectivity (Curea-selectivity) of ∼100%, as well as urea yield rates of 913.2 µg h-1 mgcat -1 and remarkable long-term stability.

12.
ACS Nano ; 17(15): 14309-14346, 2023 Aug 08.
Artículo en Inglés | MEDLINE | ID: mdl-37471703

RESUMEN

Graphdiyne (GDY), a rising star of carbon allotropes, features a two-dimensional all-carbon network with the cohybridization of sp and sp2 carbon atoms and represents a trend and research direction in the development of carbon materials. The sp/sp2-hybridized structure of GDY endows it with numerous advantages and advancements in controlled growth, assembly, and performance tuning, and many studies have shown that GDY has been a key material for innovation and development in the fields of catalysis, energy, photoelectric conversion, mode conversion and transformation of electronic devices, detectors, life sciences, etc. In the past ten years, the fundamental scientific issues related to GDY have been understood, showing differences from traditional carbon materials in controlled growth, chemical and physical properties and mechanisms, and attracting extensive attention from many scientists. GDY has gradually developed into one of the frontiers of chemistry and materials science, and has entered the rapid development period, producing large numbers of fundamental and applied research achievements in the fundamental and applied research of carbon materials. For the exploration of frontier scientific concepts and phenomena in carbon science research, there is great potential to promote progress in the fields of energy, catalysis, intelligent information, optoelectronics, and life sciences. In this review, the growth, self-assembly method, aggregation structure, chemical modification, and doping of GDY are shown, and the theoretical calculation and simulation and fundamental properties of GDY are also fully introduced. In particular, the applications of GDY and its formed aggregates in catalysis, energy storage, photoelectronic, biomedicine, environmental science, life science, detectors, and material separation are introduced.

13.
ACS Mater Au ; 2(3): 321-329, 2022 May 11.
Artículo en Inglés | MEDLINE | ID: mdl-36855385

RESUMEN

Sp-C-hybridized alkyne bonds present the natural advantages of interacting with metal atoms and have the ability to generate a large number of new catalytic active sites on the surface and the interfaces, thus greatly promoting the efficient progress of various light/electrochemical reactions. In this work, we have successfully fabricated a novel type of interfacial structure containing sp-C-Mo/O bonds and mixed Mo valence states with outstanding catalytic activity and stability for photoelectrocatalytic (PEC) overall water splitting in a wide pH range (0-14), due to the presence of sp-carbon-rich graphdiyne. For example, in alkaline conditions (pH = 14), the overpotentials of oxygen and hydrogen evolution reactions at 10 mA cm-2 are 165 and 8 mV. When being used as an electrolyzer, the cell voltage of this catalyst is only 1.40 V to achieve 10 mA cm-2. The high PEC activity of graphdiyne@molybdenum oxide originates from the conversion of chemical bonds at the sp-C hybrid interface and the coexistence of multivalent states of molybdenum, triggering a large number of catalytic active sites, greatly promoting charge transfer and lowering water dissociation energy.

14.
Nat Commun ; 13(1): 5227, 2022 Sep 05.
Artículo en Inglés | MEDLINE | ID: mdl-36064713

RESUMEN

The realization of the efficient hydrogen conversion with large current densities at low overpotentials represents the development trend of this field. Here we report the atomic active sites tailoring through a facile synthetic method to yield well-defined Rhodium nanocrystals in aqueous solution using formic acid as the reducing agent and graphdiyne as the stabilizing support. High-resolution high-angle annular dark-field scanning-transmission electron microscopy images show the high-density atomic steps on the faces of hexahedral Rh nanocrystals. Experimental results reveal the formation of stable sp-C~Rh bonds can stabilize Rh nanocrystals and further improve charge transfer ability in the system. Experimental and density functional theory calculation results solidly demonstrate the exposed high active stepped surfaces and various metal atomic sites affect the electronic structure of the catalyst to reduce the overpotential resulting in the large-current hydrogen production from saline water. This exciting result demonstrates unmatched electrocatalytic performance and highly stable saline water electrolysis.

15.
Sci Rep ; 6: 29665, 2016 07 14.
Artículo en Inglés | MEDLINE | ID: mdl-27411355

RESUMEN

SAMHD1 is a type I interferon (IFN) inducible host innate immunity restriction factor that inhibits an early step of the viral life cycle. The underlying mechanisms of SAMHD1 transcriptional regulation remains elusive. Here, we report that inducing SAMHD1 upregulation is part of an early intrinsic immune response via TLR3 and RIG-I/MDA5 agonists that ultimately induce the nuclear translocation of the interferon regulation factor 3 (IRF3) protein. Further studies show that IRF3 plays a major role in upregulating endogenous SAMHD1 expression in a mechanism that is independent of the classical IFN-induced JAK-STAT pathway. Both overexpression and activation of IRF3 enhanced the SAMHD1 promoter luciferase activity, and activated IRF3 was necessary for upregulating SAMHD1 expression in a type I IFN cascade. We also show that the SAMHD1 promoter is a direct target of IRF3 and an IRF3 binding site is sufficient to render this promoter responsive to stimulation. Collectively, these findings indicate that upregulation of endogenous SAMHD1 expression is attributed to the phosphorylation and nuclear translocation of IRF3 and we suggest that type I IFN induction and induced SAMHD1 expression are coordinated.


Asunto(s)
Inmunidad Innata/genética , Factor 3 Regulador del Interferón/genética , Proteína 1 que Contiene Dominios SAM y HD/genética , Animales , Sitios de Unión/genética , Línea Celular , Línea Celular Tumoral , Chlorocebus aethiops , Células HEK293 , Células HeLa , Humanos , Fosforilación/genética , Regiones Promotoras Genéticas/genética , Transducción de Señal/genética , Porcinos , Regulación hacia Arriba/genética
16.
PLoS One ; 11(3): e0151546, 2016.
Artículo en Inglés | MEDLINE | ID: mdl-26998839

RESUMEN

Pseudorabies (PR) is one of the most devastating diseases in the pig industry. To identify changes in microRNA (miRNA) expression and post-transcriptional regulatory responses to PRV infection in porcine kidney epithelial (PK15) cells, we sequenced a small RNA (sRNA) library prepared from infected PK15 cells and compared it to a library prepared from uninfected cells using Illumina deep sequencing. Here we found 25 novel viral miRNAs by high-throughput sequencing and 20 of these miRNAs were confirmed through stem-loop RT-qPCR. Intriguingly, unlike the usual miRNAs encoded by the α-herpesviruses, which are found clustered in the large latency transcript (LLT), these novel viral miRNAs are throughout the PRV genome like ß-herpesviruses. Viral miRNAs are predicted to target multiple genes and form a complex regulatory network. GO analysis on host targets of viral miRNAs were involved in complex cellular processes, including the metabolic pathway, biological regulation, stimulus response, signaling process and immune response. Moreover, 13 host miRNAs were expressed with significant difference after infection with PRV: 8 miRNAs were up-regulated and 5 miRNAs were down-regulated, which may affect viral replication in host cell. Our results provided new insight into the characteristic of miRNAs in response to PRV infection, which is significant for further study of these miRNAs function.


Asunto(s)
Regulación de la Expresión Génica , Herpesvirus Suido 1/fisiología , MicroARNs/genética , Seudorrabia/genética , Seudorrabia/virología , Animales , Línea Celular , Perfilación de la Expresión Génica , Biblioteca de Genes , Ontología de Genes , Redes Reguladoras de Genes , MicroARNs/metabolismo , Anotación de Secuencia Molecular , ARN Viral/genética , ARN Viral/metabolismo , Reacción en Cadena en Tiempo Real de la Polimerasa , Análisis de Secuencia de ARN , Sus scrofa
17.
Antiviral Res ; 130: 110-7, 2016 06.
Artículo en Inglés | MEDLINE | ID: mdl-26946112

RESUMEN

Emerging pseudorabies virus (PRV) variant have led to pseudorabies outbreaks in Chinese pig farms. The commercially available PRV vaccine provides poor protection against the PRV variant. In this study, a gE/gI deleted PRV strain JS-2012-△gE/gI was generated from a PRV variant strain using homologous DNA recombination. Compared to the parental strain JS-2012, JS-2012-△gE/gI grew slowly and showed small plaque morphology on Vero cells. The safety and immunological efficacy of JS-2012-△gE/gI was evaluated as a vaccine candidate. JS-2012-△gE/gI was avirulent to suckling piglets, but was able to provide full protection for young piglets against challenge with both the classical virulent PRV and the emerging PRV variant. After sows were vaccinated with the gE/gI-deleted strain, their suckling offspring were resistant to an otherwise lethal challenge with the classical and the variant PRVs. Piglets inoculated with JS-2012-△gE/gI did not develop PRV-specific gE-ELISA antibodies. Thus, JS-2012-△gE/gI appears to be a promising marker vaccine candidate to control PRV variant circulating in pig farms in China.


Asunto(s)
Eliminación de Gen , Herpesvirus Suido 1/genética , Herpesvirus Suido 1/inmunología , Seudorrabia/inmunología , Vacunas Atenuadas/inmunología , Proteínas Virales/genética , Proteínas Virales/inmunología , Vacunas Virales/inmunología , Animales , Anticuerpos Antivirales/sangre , Anticuerpos Antivirales/inmunología , Femenino , Orden Génico , Ingeniería Genética , Genoma Viral , Herpesvirus Suido 1/clasificación , Inmunidad Materno-Adquirida , Inmunización , Embarazo , Seudorrabia/prevención & control , Seudorrabia/virología , Porcinos , Enfermedades de los Porcinos/inmunología , Enfermedades de los Porcinos/prevención & control , Vacunas Atenuadas/genética , Vacunas Virales/genética
18.
Food Chem ; 173: 133-40, 2015 Apr 15.
Artículo en Inglés | MEDLINE | ID: mdl-25466004

RESUMEN

Seeds from Paeonia ostii and Paeoniarockii have been recently identified as novel resources of α-linolenic acid (ALA) in China. To assess whether tree peony cultivars can be used as oil resource, fatty acids (FAs) in 60 cultivars were monitored and evaluated in this study. The results indicated that the composition and content of FAs varied dramatically among different cultivars, in which ALA, linoleic acid, oleic acid, palmitic acid, and stearic acid were the dominant. The 60 cultivars were classified into six clusters by hierarchical cluster analysis, and they were quite distinct from each other. Finally, six cultivars with high yield and high quality were screened out, comprising of 'Liuliguanzhu', 'Hongguanyupei', 'LSS-2', 'LSS-1', 'Jingshenhuanfa' and 'LSS-11'. These cultivars were appropriately applied in practical oil production. Overall, tree peony oil with abundant unsaturated fatty acids especially ALA was proved to be a top-grade source for edible oil and nutritional supplements.


Asunto(s)
Ácidos Grasos/química , Paeonia/química , Aceites de Plantas/química , China , Cromatografía de Gases y Espectrometría de Masas/métodos , Paeonia/clasificación , Semillas/química
19.
PLoS One ; 9(10): e108860, 2014.
Artículo en Inglés | MEDLINE | ID: mdl-25279809

RESUMEN

Flavonoids in nine tissues of Nelumbo nucifera Gaertner were identified and quantified by high-performance liquid chromatography with diode array detector (HPLC-DAD) and HPLC-electrospray ionization-mass spectrometry (HPLC-ESI-MSn). Thirty-eight flavonoids were identified; eleven C-glycosides and five O-glycosides were discovered for the first time in N. nucifera. Most importantly, the C-glycosyl apigenin or luteolin detected in lotus plumules proved valuable for deep elucidation of flavonoid composition in lotus tissues and for further utilization as functional tea and medicine materials. Lotus leaves possessed the significantly highest amount of flavonoids (2.06E3±0.08 mg 100 g(-1) FW) and separating and purifying the bioactive compound, quercetin 3-O-glucuronide, from leaves showed great potential. In contrast, flavonoids in flower stalks, seed coats and kernels were extremely low. Simultaneously, the optimal picking time was confirmed by comparing the compound contents in five developmental phases. Finally, we proposed the putative flavonoid biosynthesis pathway in N. nucifera.


Asunto(s)
Flavonas/metabolismo , Flavonoides/metabolismo , Glicósidos/metabolismo , Nelumbo/metabolismo , Cromatografía Líquida de Alta Presión , Flavonas/análisis , Flavonoides/análisis , Flores/química , Glicósidos/análisis , Hojas de la Planta/química , Espectrometría de Masa por Ionización de Electrospray
20.
PLoS One ; 7(4): e34335, 2012.
Artículo en Inglés | MEDLINE | ID: mdl-22485167

RESUMEN

Water lily, the member of the Nymphaeaceae family, is the symbol of Buddhism and Brahmanism in India. Despite its limited researches on flower color variations and formation mechanism, water lily has background of blue flowers and displays an exceptionally wide diversity of flower colors from purple, red, blue to yellow, in nature. In this study, 34 flavonoids were identified among 35 tropical cultivars by high-performance liquid chromatography (HPLC) with photodiode array detection (DAD) and electrospray ionization mass spectrometry (ESI-MS). Among them, four anthocyanins: delphinidin 3-O-rhamnosyl-5-O-galactoside (Dp3Rh5Ga), delphinidin 3-O-(2"-O-galloyl-6"-O-oxalyl-rhamnoside) (Dp3galloyl-oxalylRh), delphinidin 3-O-(6"-O-acetyl-ß-glucopyranoside) (Dp3acetylG) and cyanidin 3- O-(2"-O-galloyl-galactopyranoside)-5-O-rhamnoside (Cy3galloylGa5Rh), one chalcone: chalcononaringenin 2'-O-galactoside (Chal2'Ga) and twelve flavonols: myricetin 7-O-rhamnosyl-(1 → 2)-rhamnoside (My7RhRh), quercetin 7-O-galactosyl-(1 → 2)-rhamnoside (Qu7GaRh), quercetin 7-O-galactoside (Qu7Ga), kaempferol 7-O-galactosyl-(1 → 2)-rhamnoside (Km7GaRh), myricetin 3-O-galactoside (My3Ga), kaempferol 7-O-galloylgalactosyl-(1 → 2)-rhamnoside (Km7galloylGaRh), myricetin 3-O-galloylrhamnoside (My3galloylRh), kaempferol 3-O-galactoside (Km3Ga), isorhamnetin 7-O-galactoside (Is7Ga), isorhamnetin 7-O-xyloside (Is7Xy), kaempferol 3-O-(3"-acetylrhamnoside) (Km3-3"acetylRh) and quercetin 3-O-acetylgalactoside (Qu3acetylGa) were identified in the petals of tropic water lily for the first time. Meanwhile a multivariate analysis was used to explore the relationship between pigments and flower color. By comparing, the cultivars which were detected delphinidin 3-galactoside (Dp3Ga) presented amaranth, and detected delphinidin 3'-galactoside (Dp3'Ga) presented blue. However, the derivatives of delphinidin and cyanidin were more complicated in red group. No anthocyanins were detected within white and yellow group. At the same time a possible flavonoid biosynthesis pathway of tropical water lily was presumed putatively. These studies will help to elucidate the evolution mechanism on the formation of flower colors and provide theoretical basis for outcross breeding and developing health care products from this plant.


Asunto(s)
Antocianinas/metabolismo , Flores/metabolismo , Nymphaea/metabolismo , Pigmentos Biológicos/metabolismo , Antocianinas/química , Antocianinas/aislamiento & purificación , Vías Biosintéticas , Chalcona/química , Chalcona/aislamiento & purificación , Chalcona/metabolismo , Flavonoides/química , Flavonoides/aislamiento & purificación , Flavonoides/metabolismo , Flavonoles/química , Flavonoles/aislamiento & purificación , Flavonoles/metabolismo , Glicósidos/metabolismo , Estructura Molecular , Pigmentos Biológicos/química , Pigmentos Biológicos/aislamiento & purificación
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