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1.
Pestic Biochem Physiol ; 198: 105710, 2024 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-38225068

RESUMEN

Aedes aegypti, the primary vector responsible for transmitting dengue fever in southern Taiwan, has developed a relatively high resistance to synthetic pyrethroids. It has evolved four amino acid substitutions in the voltage-gated sodium channel (VGSC), namely S996P, V1023G, F1565C, and D1794Y. To unveil the distribution and correlation of VGSC mutations and pyrethroid resistance among different field populations, Ae. aegypti collected from various districts in Kaohsiung and Tainan Cities underwent tests for resistance development against different pyrethroids and frequency of S996P, V1023G, F1565C, and D1794Y substitutions. The adult knockdown assay revealed a relatively high knockdown resistance in the Ae. aegypti populations from Kaohsiung and Tainan against permethrin, cypermethrin, and fenvalerate (averaging >50-fold). Conversely, less resistance was observed against α-cypermethrin, deltamethrin, λ-cyhalothrin, cyfluthrin, and etofenprox (averaging <35-fold). Using Polymerase Chain Reaction/restriction fragment length polymorphism analysis, four mutant haplotypes were identified in these field populations. Notably, the SIAVFD and SIBVFD wild haplotypes were absent. Analysis utilizing IBM SPSS Statistics 20.0 and Spearman's rank correlation coefficient indicated that Haplotype C (PIAGFD), especially P allele, frequency displayed a significant positive correlation with five Type II pyrethroid resistance, while 1023G and 1023G/G exhibited a significant association with permethrin and fevalerate resistance. Conversely, Haplotype E (SIBVCD) negatively correlated with pyrethroid resistance, particularly fenvalerate resistance (-0.776). Haplotype C and E were the most prevalent and widely distributed among the investigated field populations. This prevalence of haplotype C is likely tied to the extensive and excessive use of Type II pyrethroids for dengue control over the past three decades. Given the significant positive correlation, the best-fit lines and R2 values were established to facilitate the swift prediction of knockdown resistance levels to various pyrethroids based on VGSC mutation frequency. This predictive approach aims to guide insecticide usage and the management of pyrethroid resistance in the field populations of Ae. aegypti in Taiwan.


Asunto(s)
Aedes , Insecticidas , Nitrilos , Piretrinas , Canales de Sodio Activados por Voltaje , Animales , Permetrina , Aedes/genética , Aedes/metabolismo , Tasa de Mutación , Resistencia a los Insecticidas/genética , Piretrinas/farmacología , Piretrinas/metabolismo , Insecticidas/farmacología , Insecticidas/metabolismo , Mutación , Canales de Sodio Activados por Voltaje/genética , Canales de Sodio Activados por Voltaje/metabolismo , Mosquitos Vectores/genética
2.
Angew Chem Int Ed Engl ; 62(9): e202216776, 2023 Feb 20.
Artículo en Inglés | MEDLINE | ID: mdl-36524754

RESUMEN

Recent advances in perovskite ferroelectrics have fostered a host of exciting sensors and actuators. Defect engineering provides critical control of the performance of ferroelectric materials, especially lead-free ones. However, it remains a challenge to quantitatively study the concentration of defects due to the complexity of measurement techniques. Here, a feasible approach to analyzing the A-site defect and electron in alkali metal niobate is demonstrated. The theoretical relationships among defect concentration, conductivity, and oxygen partial pressure can be established based on the defect chemistry equilibria. The type and concentration of defects are reflected through the conductivity variation with oxygen partial pressure. As a result, the variation of defect concentration gives rise to defect-driven interfacial polarization, which further leads to distinct properties of the ceramics. e.g., abnormal dielectric behavior. Furthermore, this study also suggests a strategy to manipulate defects and charges in perovskite oxides for performance optimization.

3.
J Gastroenterol Hepatol ; 36(2): 474-481, 2021 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-32677707

RESUMEN

BACKGROUND AND AIM: Remimazolam tosilate (RT) is a new short-acting GABA(A) receptor agonist, having potential to be an effective option for procedural sedation. Here, we aimed to compare the efficacy and safety of RT with propofol in patients undergoing upper gastrointestinal endoscopy. METHODS: This positive-controlled, non-inferiority, phase III trial recruited patients at 17 centers, between September 2017 and November 2017. A total of 384 patients scheduled to undergo upper gastrointestinal endoscopy were randomly assigned to receive RT or propofol. Primary endpoint was the success rate of sedation. Adverse events (AEs) were recorded to evaluate safety. RESULTS: The success rate of sedation in the RT group was non-inferior to that in the propofol group (97.34% vs 100.00%; difference in rate -2.66%, 95% CI -4.96 to -0.36, meeting criteria for non-inferiority). Patients in the RT group had longer time to adequate sedation (P < 0.0001) but shorter time to fully alert (P < 0.0001) than that in the propofol group. The incidences of hypotension (13.04% vs 42.86%, P < 0.0001), treatment-related hypotension (0.54% vs 5.82%, P < 0.0001), and respiratory depression (1.09% vs 6.88%, P = 0.0064) were significantly lower in the RT group. AEs were reported in 74 (39.15%) patients in the RT group and 114 (60.32%) patients in the propofol group, with significant difference (P < 0.0001). CONCLUSION: This trial established non-inferior sedation success rate of RT compared with propofol. RT allows faster recovery from sedation compared with propofol. The safety profile is favorable and appears to be superior to propofol, indicating that it was feasible and well tolerated for patients.


Asunto(s)
Benzodiazepinas/administración & dosificación , Sedación Consciente/métodos , Endoscopía Gastrointestinal , Adulto , Anciano , Periodo de Recuperación de la Anestesia , Benzodiazepinas/efectos adversos , Estudios de Factibilidad , Femenino , Humanos , Hipertensión/inducido químicamente , Hipertensión/epidemiología , Hipnóticos y Sedantes/administración & dosificación , Hipnóticos y Sedantes/efectos adversos , Incidencia , Masculino , Persona de Mediana Edad , Propofol/administración & dosificación , Propofol/efectos adversos , Insuficiencia Respiratoria/inducido químicamente , Insuficiencia Respiratoria/epidemiología , Seguridad
4.
Zhonghua Nan Ke Xue ; 24(8): 713-718, 2018 Aug.
Artículo en Zh | MEDLINE | ID: mdl-30173431

RESUMEN

OBJECTIVE: To evaluate the efficacy and safety of injection of botulinum-A toxin into the bulbospongiosus muscle in the treatment of primary premature ejaculation (PPE). METHODS: According to the inclusion criteria, we randomly assigned 70 outpatients with PPE to a trial and a control group of equal number, the former injected with 100 U botulinum-A toxin at 10 U/ml and the latter with the same volume of saline into the bulbospongiosus muscle. Then, we obtained the intravaginal ejaculatory latency time (IELT), scores of the Premature Ejaculation Profile (PEP), Male Sexual Health Questionnaire-Ejaculatory Dysfunction (MSHQ-EjD), and Hospital Anxiety and Depression Scale (HADS), and the incidence of adverse reactions between the two groups before and 4 weeks after treatment. RESULTS: Complete data were obtained from 69 of the patients, 34 in the trial and 35 in the control group. The effectiveness rate was 47.06% (16/34) in the former but 0 in the latter. At 4 weeks after treatment, the patients of the trial group showed a significantly longer IELT than the controls and the baseline (ï¼»2.35 ± 1.83ï¼½ vs ï¼»0.79 ± 0.21ï¼½ and ï¼»0.74 ±+ 0.27ï¼½ min, P < 0.01) and the controls. The patients in the trial group, in comparison with those in the saline control group and the baseline, also exhibited significant improvement in the scores of PEP-ejaculation control (1.21 ± 1.04 vs 0.49 ± 0.56 and 0.47 ± 0.51, P < 0.05), PEP-sexual satisfaction (1.32 ± 1.01 vs 0.71 ± 0.57 and 0.79 ± 0.48, P < 0.05), PEP-PE-related distress (2.12 ± 1.01 vs 2.80 ± 0.68 and 2.76 ± 1.26, P < 0.05), and PEP-PE-induced difficult relationship with the partners (1.38 ± 0.70 vs 2.37 ± 0.55 and 2.12 ± 1.49, P < 0.05). The sexual satisfaction score of the female partners after treatment was markedly improved in the trial group as compared with the control group and the baseline (1.18 ± 1.00 vs 0.57 ± 0.50 and 0.62 ± 0.60, P < 0.05). There were no statistically significant differences in MSHQ-EjD and HADS scores between the two groups before and after treatment. Adverse reactions were observed in 6 cases (17.65%) in the trial group, including 4 cases of decreased erectile hardness (11.76%) and 2 cases of incomplete urination (5.88%), which occurred from the 3 to 4 days after injection, and those with decreased erectile hardness could complete sexual intercourse without any other treatment and recovered after 3 weeks. CONCLUSIONS: Injection of botulinum-A toxin into the bulbospongiosus muscle can be used as an option for the treatment of PPE. Its clinical application value, however, needs to be verified by further studies with larger samples.


Asunto(s)
Toxinas Botulínicas Tipo A/administración & dosificación , Fármacos Neuromusculares/administración & dosificación , Perineo , Eyaculación Prematura/tratamiento farmacológico , Coito , Eyaculación/efectos de los fármacos , Femenino , Humanos , Inyecciones Intramusculares , Masculino , Parejas Sexuales , Encuestas y Cuestionarios
5.
Future Oncol ; 13(17): 1505-1516, 2017 Jul.
Artículo en Inglés | MEDLINE | ID: mdl-28766961

RESUMEN

AIM: To investigate the clinical relevance and functional role of HOXA13 in prostate cancer Methods: PCR, western blot and immunohistochemistry were performed to determine the expression. Kaplan-Meier and Cox regression survival analyses investigated the clinical relevance. Cell viability, flow cytometry and transwell assays were used to determine the functional roles. RESULTS: HOXA13 expression is sharply increased in carcinoma tissues and is significantly associated with poor prognosis of prostate cancer patients. Interestingly, nucleus not cytoplasm HOXA13 expression is associated with unfavorable survival of the patients. Furthermore, nucleus HOXA13 expression represents an unfavorable and independent prognosis factor of histological grade 2 or Gleason grade <8 patients. Functionally, forced expression of HOXA13 obviously promotes tumor cell proliferation, migration and invasion, whereas inhibits tumor cell apoptosis. CONCLUSION: HOXA13 is an unfavorable prognostic factor and a novel oncogene for prostate cancer.


Asunto(s)
Carcinoma/genética , Proteínas de Homeodominio/genética , Oncogenes/genética , Neoplasias de la Próstata/genética , Anciano , Anciano de 80 o más Años , Apoptosis/genética , Biomarcadores de Tumor/genética , Carcinoma/epidemiología , Carcinoma/patología , Línea Celular Tumoral , Proliferación Celular/genética , Supervivencia sin Enfermedad , Regulación Neoplásica de la Expresión Génica , Predisposición Genética a la Enfermedad , Humanos , Estimación de Kaplan-Meier , Masculino , Persona de Mediana Edad , Pronóstico , Neoplasias de la Próstata/epidemiología , Neoplasias de la Próstata/patología
6.
Zhonghua Nan Ke Xue ; 23(3): 237-242, 2017 Mar.
Artículo en Zh | MEDLINE | ID: mdl-29706045

RESUMEN

OBJECTIVE: To search for an optimal strategy for the treatment of penile and scrotal gangrene by analyzing the clinical effect of vacuum sealing drainage (VSD) as an adjuvant treatment on this disease. METHODS: We retrospectively analyzed the clinical data about 4 cases of penile and scrotal gangrene treated by VSD as an adjuvant treatment from January 2015 to June 2016. The 4 patients all underwent early extensive and radical debridement of gangrene of the scrotum and penis and received intravenous injection of two broad-spectrum antibiotics, followed by VSD for wound drainage and irrigation. RESULTS: Adequate wound drainage was achieved in all the 4 cases, the gangrene range rapidly localized and testicular necrosis avoided. The wound surface healed satisfactorily after cleansing and suturing. The patients were followed up for 3 months after discharged from the hospital and none experienced recurrence. CONCLUSIONS: VSD combined with early adequate debridement can effectively localize the gangrene range, significantly reduce the frequency of changing dressings and shorten the hospitalization time of the patient, and therefore is a very effective adjuvant treatment of penile and scrotal gangrene.


Asunto(s)
Gangrena/terapia , Enfermedades de los Genitales Masculinos/terapia , Terapia de Presión Negativa para Heridas/métodos , Pene/patología , Escroto/patología , Desbridamiento , Enfermedades de los Genitales Masculinos/patología , Enfermedades de los Genitales Masculinos/prevención & control , Humanos , Masculino , Estudios Retrospectivos , Testículo/patología , Resultado del Tratamiento , Vacio
7.
Chirality ; 26(2): 121-7, 2014 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-24420919

RESUMEN

A doubly stereocontrolled organocatalytic asymmetric Michael addition to the synthesis of substituted succinimides is described. Starting from aldehydes and maleimides, both enantiomers of the succinimides could be obtained in high to excellent yields (up to 98%) and enantioselectivities (up to 99%) when one of the two special chiral diterpene-derived bifunctional thioureas was individually used as a catalyst. Moreover, these catalysts can be efficiently used in large-scale catalytic synthesis with the same level of yield and enantioselectivity.


Asunto(s)
Diterpenos/química , Succinimidas/química , Tiourea/química , Catálisis , Estructura Molecular , Estereoisomerismo
8.
Hepatogastroenterology ; 60(121): 79-82, 2013.
Artículo en Inglés | MEDLINE | ID: mdl-22773302

RESUMEN

BACKGROUND/AIMS: To investigate the influence of different anesthesia methods on liver and renal function in elderly patients undergoing laparoscopic colon or rectal resection. METHODOLOGY: Forty ASA I-II elderly patients undergoing elective laparoscopic colon or rectal resection were randomly divided into 2 groups (n=20 each): Group A: routine general anesthesia and Group B: general combined epidural anesthesia. All patients received general anesthesia, tracheal catheterized and mechanical ventilation. Data of liver and renal function were noted at these time points: before operation (T1), during operation (T2), 1-day after operation (T3), 2-day after operation during bowel anastomoses (T4), 3-day after operation (T5). Duration of surgery, blood loss, urine output and intravenous fluid volume was registered. RESULTS: Compared with group A, the total fluid volume and urine volume were increased in group B (p<0.05), while the amount of fentanyl was decreased (p<0.05). TP, TBIL and DBIL between the two groups were not significantly different (p>0.05). Compared with group A, group B ALT/BUN decreased at T2 and T3, AST decreased at T2, Cr at T3 decreased, ALB decreased at T2 (p<0.05). CONCLUSIONS: Continuous epidural block compound general anesthesia in elderly laparoscopic colorectal resection surgery is more conducive to the protection of perioperative liver and kidney function.


Asunto(s)
Anestesia/métodos , Colon/cirugía , Riñón/fisiopatología , Laparoscopía , Hígado/fisiopatología , Recto/cirugía , Anciano , Anciano de 80 o más Años , Femenino , Humanos , Masculino , Persona de Mediana Edad
9.
J Hepatocell Carcinoma ; 10: 1827-1837, 2023.
Artículo en Inglés | MEDLINE | ID: mdl-37850079

RESUMEN

Purpose: Few reliable biomarkers for predicting the efficacy of triple therapy (lenvatinib + immune checkpoint inhibitors + transarterial chemoembolization) exist for patients with unresectable hepatocellular carcinoma (uHCC). This study explored the prognostic role of alpha-fetoprotein (AFP) and des-gamma-carboxyprothrombin (DCP) levels in patients with uHCC receiving triple therapy. Patients and Methods: This retrospective study included 93 patients with uHCC who received triple therapy at Fujian Provincial Hospital between August 2020 and November 2022. Depending on the respective baseline levels, the patients were divided into high-AFP and high-DCP groups. An early response was defined as an AFP or DCP concentration >50% less than the baseline concentration after 6 weeks of triple therapy. The primary endpoint was the objective response rate (ORR). The secondary endpoints were progression-free survival (PFS) and overall survival (OS). Results: After 6 weeks of triple therapy, 75.3% (58/77) and 78.9% (60/76) of patients in the high-AFP and high-DCP groups achieved an objective response. Early AFP and DCP responses were positively associated with ORR (high-AFP group: odds ratio [OR]: 13.542; 95% confidence interval [CI]: 3.991-45.950, p<0.001; high-DCP group: OR: 17.853; 95% CI: 4.478-71.179, p<0.001). In the high-AFP group, the 6-month, 12-month, and 18-month PFS and OS rates were higher in the AFP responders than those in the non-responders (PFS: 66.4%, 59.6%, 48.2% vs 42.3%, 19.3%, 0%, p<0.001; OS: 94.5%, 90.4%, 77.3% vs 75.6%, 66.2%, 49.6%, p=0.006). In the high-DCP group, the 6-month, 12-month, and 18-month PFS and OS rates were higher in the DCP responders than those in the non-responders (PFS: 67.4%, 57.7%, 39.0% vs 38.9%, 8.1%, 0%, p<0.001; OS: 94.7%, 94.7%, 83.3% vs 77.0%, 53.9%, 36.0%, p<0.001). Conclusion: After 6 weeks of triple therapy, an AFP or DCP reduction of >50% predicts better treatment outcomes in uHCC patients.

10.
Hepatogastroenterology ; 59(120): 2457-60, 2012.
Artículo en Inglés | MEDLINE | ID: mdl-22626878

RESUMEN

To investigate the effect of stroke volume variability(SVV)-guided intraoperative fluid restriction on gastrointestinal functional recovery and postoperative outcome after gastrointestinal surgery. Forty ASA I-II patients undergoing elective gastrointestinal surgery were randomly divided into 2 groups (n=20 each):group A routine fluid administration and group B restricted fluid administration. SW value was maintained at 5-7 in group A and 11-13 in group B. All patients received general anesthesia, tracheal catheterized and mechanical ventilation. Hemodynamic monitoring parameters (MBP, HR, CVP, CO) were noted at 5 time points. The hemodynamic parameters were maintained within normal limits during operation in both groups. The intraoperative intravenous fluid volume in group B was significant less than in group A (p<0.01).Urine output in group A was significant less than in group B (p<0.05). Stroke volume variation measured from Edwards Flotrac sensor and Edwards Vigileo monitor could be a security and sensitive parameter as an index of volume administration. In elective gastrointestinal surgery, volume resuscitation with a goal SVV of11-13 not only reduced intravenous fluid volume but also maintained the stable hemodynamic and tissue perfusion, enhanced gastrointestinal functional recovery and reduced the length of hospital stay


Asunto(s)
Procedimientos Quirúrgicos del Sistema Digestivo , Fluidoterapia , Monitoreo Intraoperatorio/métodos , Volumen Sistólico , Adulto , Anciano , Análisis de Varianza , Presión Sanguínea , Volumen Sanguíneo , China , Procedimientos Quirúrgicos del Sistema Digestivo/efectos adversos , Procedimientos Quirúrgicos Electivos , Femenino , Fluidoterapia/efectos adversos , Humanos , Tiempo de Internación , Masculino , Persona de Mediana Edad , Monitoreo Intraoperatorio/instrumentación , Valor Predictivo de las Pruebas , Recuperación de la Función , Respiración Artificial , Factores de Tiempo , Transductores de Presión , Resultado del Tratamiento , Micción
11.
Zhonghua Yi Xue Za Zhi ; 92(5): 316-9, 2012 Feb 07.
Artículo en Zh | MEDLINE | ID: mdl-22490834

RESUMEN

OBJECTIVE: To evaluate the pre- and intraoperative risk factors associated with excessive bleeding during the perioperative period in adult patients undergoing open heart surgery with cardiopulmonary bypass (CPB). METHODS: A total of 1079 consecutive patients undergoing open heart surgery with CPB from January 2001 to May 2010 were included (except for emergency operation). The possible risk factors associated with excessive bleeding were retrospectively analyzed. Patients who received ≥ 7 units of RBC or had a re-operation during which no active bleeding point was found within one day of operation were classified as excessive bleeding. According to the occurrence of excessive bleeding, they were divided into 2 groups: excessive and non-excessive bleeding groups. The possible risk factors associated with excessive bleeding were retrospectively analyzed. Univariate analysis and multivariate Logistic regression analysis were used to examine the relationship between these factors and excessive bleeding. RESULTS: Among them, 120 (11.1%) developed excessive bleeding. Multivariate Logistic analysis indicated that the risk factors for excessive bleeding were age (OR = 4.533, 95%CI 2.624 - 7.831), previous sternotomy (OR = 2.781, 95%CI 1.410 - 5.486), preoperative hematocrit concentration (OR = 0.896, 95%CI 0.861 - 0.932), CPB duration (OR = 2.782, 95%CI 1.791 - 4.322) and type of procedure (OR = 2.292, 95%CI 1.376 - 3.817). CONCLUSION: Age ≥ 65 years, previous sternotomy, preoperative low hematocrit concentration, CPB duration ≥ 120 min and complex operation were the significant predictors for excessive bleeding in patient undergoing open heart surgery with CPB.


Asunto(s)
Pérdida de Sangre Quirúrgica , Puente Cardiopulmonar/efectos adversos , Hemorragia Posoperatoria/etiología , Adolescente , Adulto , Anciano , Anciano de 80 o más Años , Femenino , Humanos , Modelos Logísticos , Masculino , Persona de Mediana Edad , Análisis Multivariante , Estudios Retrospectivos , Factores de Riesgo , Adulto Joven
12.
Zhonghua Yi Xue Za Zhi ; 91(30): 2132-4, 2011 Aug 16.
Artículo en Zh | MEDLINE | ID: mdl-22093991

RESUMEN

OBJECTIVE: To evaluate whether combined oral oxycodone hydrochloride controlled-release tablets plus paracetamol and tramadol hydrochloride tablets is better than epidural analgesia with respect to uterine cramping pain control and side effects after cesarean section. METHODS: Sixty consecutive patients scheduled for cesarean section from April to May, 2010 were randomized to either patient-controlled epidural analgesia with 0.1% ropivacaine, 0.1 µg/ml sufentanil (for postoperative 48 h) plus injected pethidine on demand (EDA group) or controlled-release oxycodone (2 × 15 mg for 1st postoperative 24 h; 2 × 10 mg for 2nd postoperative 24 h), paracetamol & tramadol hydrochloride tablets (8 × 1 tablet for postoperative 48 h) orally plus pethidine injection on demand (OXY group). Two groups were compared with respects to uterine cramping pain control when the oxytocin infusion (20 U plus 500 ml 5% glucose solution, iv. gtt within 2 h) once per day for postoperative 3 days as determined by the means of a visual analogue scale (VAS), pethidine consumption, side effects and costs. RESULTS: The EDA group experienced significant more pain than the OXY group when the oxytocin infusion was administered (mm) [50.0 (15.0, 72.5) vs 25.0 (0, 40.0), 60.0 (47.5, 72.5) vs 20.0 (0, 30.0), 35.0 (20.0, 50.0) vs 0 (0, 20.0)]. all P < 0.05). Pethidine was used for pain control in 2 patients (150 mg total) of EDA group during the oxytocin infusion whereas none of the OXY group received an injection of pethidine. There was a higher level of maternal satisfaction with a lower analgesic dose in the EDA group (80.9 ± 9.3 vs 90.0 ± 9.8, P < 0.01). The median duration of hospital stay was around 5 days in both groups. CONCLUSION: Postoperative pain control after cesarean section with the above combined regimen is superior to EDA in terms of a lower cost and a higher level of maternal satisfaction.


Asunto(s)
Analgesia Obstétrica/métodos , Oxicodona/uso terapéutico , Dolor Postoperatorio/tratamiento farmacológico , Adulto , Analgesia Controlada por el Paciente , Cesárea , Femenino , Humanos , Oxicodona/administración & dosificación
13.
Aging Cell ; 20(5): e13343, 2021 05.
Artículo en Inglés | MEDLINE | ID: mdl-33721419

RESUMEN

Although important factors governing the meiosis have been reported in the embryonic ovary, meiosis in postnatal testis remains poorly understood. Herein, we first report that SRY-box 30 (Sox30) is an age-related and essential regulator of meiosis in the postnatal testis. Sox30-null mice exhibited uniquely impaired testis, presenting the abnormal arrest of germ-cell differentiation and irregular Leydig cell proliferation. In aged Sox30-null mice, the observed testicular impairments were more severe. Furthermore, the germ-cell arrest occurred at the stage of meiotic zygotene spermatocytes, which is strongly associated with critical regulators of meiosis (such as Cyp26b1, Stra8 and Rec8) and sex differentiation (such as Rspo1, Foxl2, Sox9, Wnt4 and Ctnnb1). Mechanistically, Sox30 can activate Stra8 and Rec8, and inhibit Cyp26b1 and Ctnnb1 by direct binding to their promoters. A different Sox30 domain required for regulating the activity of these gene promoters, providing a "fail-safe" mechanism for Sox30 to facilitate germ-cell differentiation. Indeed, retinoic acid levels were reduced owing to increased degradation following the elevation of Cyp26b1 in Sox30-null testes. Re-expression of Sox30 in Sox30-null mice successfully restored germ-cell meiosis, differentiation and Leydig cell proliferation. Moreover, the restoration of actual fertility appeared to improve over time. Consistently, Rec8 and Stra8 were reactivated, and Cyp26b1 and Ctnnb1 were reinhibited in the restored testes. In summary, Sox30 is necessary, sufficient and age-associated for germ-cell meiosis and differentiation in testes by direct regulating critical regulators. This study advances our understanding of the regulation of germ-cell meiosis and differentiation in the postnatal testis.


Asunto(s)
Factores de Transcripción SOX/fisiología , Espermatozoides/citología , Testículo/citología , Envejecimiento , Animales , Diferenciación Celular , Línea Celular , Proliferación Celular , Regulación de la Expresión Génica , Masculino , Meiosis , Profase Meiótica I , Ratones , Regiones Promotoras Genéticas , Dominios Proteicos , Factores de Transcripción SOX/química , Factores de Transcripción SOX/genética , Factores de Transcripción SOX/metabolismo , Diferenciación Sexual , Testículo/metabolismo , Tretinoina/metabolismo
14.
Zhonghua Yi Xue Za Zhi ; 90(1): 49-52, 2010 Jan 05.
Artículo en Zh | MEDLINE | ID: mdl-20356526

RESUMEN

OBJECTIVE: To compare the variations of hemodynamics or respiratory parameters and postoperative complications incidence in gynecological laparoscopy to perform Laryngeal Mask Airway (LMA), Streamlined Liner of the Pharynx Airway (SLIPA) or Tracheal Tube (TT) and to appraise the safety or superiority SLIPA in gynecological laparoscopy. METHODS: 90 patients as ASA I-II grade for gynecological laparoscopy to divide randomly into three groups (n = 30): SLIPA group(S), LMA group(L), TT group(T). Patients were intubated with SLIPA, LMA or TT respectively after general anesthesia induction. Record and count baseline or variable values on heart rate, mean arterial pressure, peak inspiratory pressure, flat inspiratory pressure, airway compliance, airway resistance as pre- and post-intubation. To observe intra- and post-complications on backflow or aspiration, airway shifting, oral mucosa damage, cough or expectoration, pharyngodynia, trachyphonia and to appraise patient's comfort as extubation. RESULTS: S or L group is more stable than T on hemodynamic (P < 0.05) during intubation, S group is more stable than L or T group on respiratory parameters (P < 0.05). Intraoperative incidences are significantly lower in S group than L group on backflow or aspiration, airway shifting or oral mucosa damage (P < 0.05), postoperative incidences are significantly lower in S group than L or T group on pharyngodynia, trachyphonia or cough or expectoration (P < 0.05). In addition, extubation comfort degree is better to patient as resuscitation (P < 0.05). CONCLUSIONS: SLIPA are safely or conveniently applicable in gynecological laparoscopy. It impacts little on variations of hemodynamics or respiratory parameters after general anesthesia and reduces intra- or post-operative complications significantly compared to L or T group, increases patient's comfort as extubation. SLIPA is superior to other two on clinical applications.


Asunto(s)
Procedimientos Quirúrgicos Ginecológicos/métodos , Laparoscopía/métodos , Máscaras Laríngeas , Adolescente , Adulto , Anciano , Femenino , Procedimientos Quirúrgicos Ginecológicos/instrumentación , Humanos , Persona de Mediana Edad , Ventilación Pulmonar , Adulto Joven
15.
Zhonghua Yi Xue Za Zhi ; 90(11): 760-2, 2010 Mar 23.
Artículo en Zh | MEDLINE | ID: mdl-20627022

RESUMEN

OBJECTIVE: To evaluate whether administration of flurbiprofen axetil before elective laparoscopic cholecystectomy can affect the BIS index and hemodynamics. METHOD: Sixty patients scheduled for elective laparoscopic cholecystectomy were randomized into two groups. Flurbiprofen axetil 1 mg/kg was used intravenous 15 minutes before induction of general anesthesia in group B, while group A without premedication. Total intravenous anesthesia was maintained with propofol TCI. BIS index, blood pressure and heart rate were recorded at the time just before induction, after endotracheal intubation, 5 minutes before incision, incision, 5, 10, 15, 20 and 25 mins after incision. RESULT: There were no significant difference between two groups in BIS index during period of anesthesia and awareness (all P > 0.05). Compared with group A, both systolic and diastolic blood pressure in group B at time of incision (T(1)) are apparently lower (P < 0.05). While, blood pressures were no significant different at other time (all P > 0.05). CONCLUSION: In this study, administration of flurbiprofen before surgery of laparoscopic cholecystectomy did not alter BIS and the degree of sedation during total intravenous anesthesia. But it can reduce the harmful stimulation to cardiovascular reaction from surgery and make hemodynamic status more stable. Flurbiprofen can be safely and effectively used on elective laparoscopic cholecystectomy.


Asunto(s)
Anestesia Intravenosa/métodos , Anestésicos Intravenosos/uso terapéutico , Colecistectomía Laparoscópica/métodos , Flurbiprofeno/análogos & derivados , Adolescente , Adulto , Anciano , Femenino , Flurbiprofeno/uso terapéutico , Humanos , Masculino , Persona de Mediana Edad , Premedicación , Adulto Joven
16.
Zhonghua Yi Xue Za Zhi ; 90(23): 1631-4, 2010 Jun 15.
Artículo en Zh | MEDLINE | ID: mdl-20979754

RESUMEN

OBJECTIVE: The aim of this research is to observe whether esmolol infusion as an adjunct to propofol can affect BIS index, reduce anesthetic dose and decrease emergence time. METHOD: Sixty ASA I-II patients, age 18-35, undergoing uterine dilatation and curettage surgery were studied. They were randomized into two groups. Before induction, patients in esmolol group (Group E) were received 1 mg/kg esmolol intravenously and followed by esmolol 150 microg x kg(-1) x min(-1) intravenous infusion; patients in group C received normal saline instead of esmolol. Fentanyl (1 microg/kg) and propofol (2 mg/kg) were used as induction drugs. The change of BIS index, heart rate and MAP during operation; total amount of propofol; time when patients opened eyes and time when patients reached the standard for discharge from hospital were recorded. RESULTS: BIS and heart rate of Group C at 1,2,3 minute after surgery started, increased significantly compared with the time after induction and those in Group E (P < 0.05). The time patients reached the score of discharging from hospital in Group C is longer than that in Group E (P < 0.05). CONCLUSION: Esmolol combined with propofol administering in minor ambulatory operations can control the increase of BIS index caused by surgical nociceptive stimulus. Meanwhile the combination could reduce the dose of sedatives and decrease anesthesia emergence time.


Asunto(s)
Procedimientos Quirúrgicos Ambulatorios/métodos , Anestesia/métodos , Anestésicos Intravenosos/uso terapéutico , Propanolaminas/uso terapéutico , Aborto Inducido , Adolescente , Adulto , Periodo de Recuperación de la Anestesia , Electroencefalografía , Femenino , Humanos , Embarazo , Propofol/uso terapéutico , Adulto Joven
17.
Behav Processes ; 181: 104248, 2020 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-32961283

RESUMEN

Birth attendance, or midwifery service, is an important characteristic in human evolution, and has been argued to separate our lineage from other taxa in the animal kingdom. Recent studies, however, indicate that similar or analogous behaviors also may occur in a small number of nonhuman primate species. Here, we report the first case of both male and female attendance and female birth assistance in a wild species of nonhuman primate, the black-and-white snub-nosed monkey (Rhinopithecus bieti). At our field site in Yunnan, China we observed a diurnal birth event in which the leader male of a one-male unit (OMU) remained in close- proximity (0-2 m) to the parturient, groomed her, and remained vigilant over a five hour pre- and postpartum period. In addition, a multiparous female member of the OMU also remained in close proximity to the soon-to-be mother, helped to pull the neonate from the birth canal, took the neonate from the new mother within 15 s of the birth, held the infant for 20 min, and then severed the umbilical cord. For the next several days the leader male traveled in close-proximity to the new mother and four days after the birth event, we observed him to share food with her. Given that diurnal births are extremely rare in this primate species; it remains unclear the degree to which the events we observed commonly occur during nighttime births. We argue that adult male and female black-and-white snub-nosed monkeys are highly attracted to young infants, and birth attendance and birth assistance in this, and certain other primate species, may play a critical role in maternal and infant survivorship.


Asunto(s)
Presbytini , Animales , China , Femenino , Masculino , Madres , Parto , Periodo Posparto , Embarazo
18.
Zool Res ; 41(1): 78-83, 2020 01 18.
Artículo en Inglés | MEDLINE | ID: mdl-31746565

RESUMEN

Individual dispersal trends, unquestionably important for species ecology and evolution, are affected by multiple factors. Understanding the factors that influence female dispersal strategies offers important insight into primate dispersal mechanisms and female choice. To investigate the proximate causes of dispersal in female Yunnan snub-nosed monkeys (Rhinopithecus bieti), we observed and analyzed nine years of detailed dispersal and demographic data from a population of R. bieti in Xiangguqing, Baimaxueshan Nature Reserve, Yunnan Province, China. Results showed that females who lived long-term in a one-male unit (OMU), without giving birth and with few or no relatives, were more likely to leave that OMU. In addition, an OMU led by an outgroup male and containing more female relatives was significantly more likely to be chosen for immigration. Conversely, greater male age, longer male tenure, and more potentially fertile females discouraged immigration into an OMU. These results suggest that reproduction, male quality, and kin cooperation play the largest roles in female Yunnan snub-nosed monkey dispersal.


Asunto(s)
Distribución Animal , Conducta Animal , Presbytini/fisiología , Conducta Social , Envejecimiento , Animales , Femenino , Masculino
19.
Zhonghua Wai Ke Za Zhi ; 47(24): 1888-91, 2009 Dec 15.
Artículo en Zh | MEDLINE | ID: mdl-20193409

RESUMEN

OBJECTIVES: To measure the linear polyethylene wear with use of a computer-assisted two-dimensional method, and to evaluate the relationship between wear and long-term clinic-radiological findings. METHODS: Between December 1991 and December 1995, the senior author performed 40 primary total hip arthroplasties with use of H/G porous-coated acetabular component. Radiographs of 40 H/G cementless total hip arthroplasties were examined for osteolysis, radiolucent line, cup migration and so on. To measure the linear polyethylene wear, migration in the femoral head center relative to the cup shell center was calculated on digitized consecutive radiographs with special computer software program. RESULTS: The minimum follow-up was 10 years. Total linear wear was 2-8 mm, the mean rate of linear polyethylene wear was (0.32+/-0.31) mm/year. Twelve acetabular components (30%) were revised at an average follow-up point of 12 years. Three types of polyethylene liner failure were identified: wear-through of the polyethylene liner with resultant metallosis (5 hips), excessive wear necessitating revision (5 hips), and polyethylene liner dissociation without gross evidence of wear (2 hips). CONCLUSIONS: High wear rates and femoral and acetabular osteolysis have been and still are the main problems in uncemented total hip replacement. There is clear relationship between wear and acetabular osteolysis or loosening of the prostheses. The poor long-term results with these uncemented total hip arthroplasties illustrate the necessity of regular radiographic evaluation in order to detect osteolysis and liner failure, which are both generally asymptomatic until catastrophic failure appears.


Asunto(s)
Prótesis de Cadera , Complicaciones Posoperatorias , Falla de Prótesis , Adulto , Anciano , Artroplastia de Reemplazo de Cadera/métodos , Estudios de Seguimiento , Humanos , Persona de Mediana Edad , Osteólisis/etiología , Polietileno , Estudios Retrospectivos
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