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Heading date (flowering time), which greatly influences regional and seasonal adaptability in rice (Oryza sativa), is regulated by many genes in different photoperiod pathways. Here, we characterized a heading date gene, Early heading date 5 (Ehd5), using a modified bulked segregant analysis method. The ehd5 mutant showed late flowering under both short-day and long-day conditions, as well as reduced yield, compared to the wild type. Ehd5, which encodes a WD40 domain-containing protein, is induced by light and follows a circadian rhythm expression pattern. Transcriptome analysis revealed that Ehd5 acts upstream of the flowering genes Early heading date 1 (Ehd1), RICE FLOWERING LOCUS T 1 (RFT1), and Heading date 3a (Hd3a). Functional analysis showed that Ehd5 directly interacts with Rice outermost cell-specific gene 4 (Roc4) and Grain number, plant height, and heading date 8 (Ghd8), which might affect the formation of Ghd7-Ghd8 complexes, resulting in increased expression of Ehd1, Hd3a, and RFT1. In a nutshell, these results demonstrate that Ehd5 functions as a positive regulator of rice flowering and provide insight into the molecular mechanisms underlying heading date.
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Flores , Oryza , Ritmo Circadiano , Flores/genética , Flores/metabolismo , Regulación de la Expresión Génica de las Plantas/genética , Oryza/genética , Oryza/metabolismo , Fotoperiodo , Proteínas de Plantas/genética , Proteínas de Plantas/metabolismo , Repeticiones WD40/genéticaRESUMEN
Grain yield and grain quality are major determinants in modern breeding controlled by many quantitative traits loci (QTLs) in rice (Oryza sativa). However, the mechanisms underlying grain shape and quality are poorly understood. Here, we characterize a QTL for grain size and grain quality via map-based cloning from wild rice (W1943), GS10 (Grain Size on Chromosome 10), which encodes a protein with 6 tandem armadillo repeats. The null mutant gs10 shows slender and narrow grains with altered cell size, which has a pleiotropic effect on other agronomical traits. Functional analysis reveals that GS10 interacts with TUD1 (Taihu Dwarf1) and is epistatic to OsGSK2 (glycogen synthase kinase 2) through regulating grain shape and lamina joint inclination, indicating it is negatively involved in brassinosteroid (BR) signaling. Pyramiding gs10 and the grain size gene GW5 into cultivar GLA4 substantially improved grain shape and appearance quality. Natural variation analysis revealed that gs10 from the wild rice Oryza rufipogon W1943 is a rare allele across the rice population. Collectively, these findings advance our understanding of the underlying mechanism of grain shape and provide the beneficial allele of gs10 for future rice breeding and genetic improvement.
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Brasinoesteroides , Oryza , Brasinoesteroides/metabolismo , Grano Comestible/genética , Oryza/genética , Fenotipo , Fitomejoramiento , Sitios de Carácter Cuantitativo/genéticaRESUMEN
Wild rice (Oryza rufipogon) has a lower panicle seed setting rate (PSSR) and gamete fertility than domesticated rice (Oryza sativa), but the genetic mechanisms of this phenomenon remain unknown. Here, we cloned a null allele of OsMLH1, an ortholog of MutL-homolog 1 to yeast and mammals, from wild rice O. rufipogon W1943 and revealed a 5.4-kb retrotransposon insertion in OsMLH1 is responsible for the low PSSR in wild rice. In contrast to the wild-type, a near isogenic line NIL-mlh1 exhibits defective crossover (CO) formation during meiosis, resulting in reduced pollen viability, partial embryo lethality, and low PSSR. Except for the mutant of mismatch repair gene postmeiotic segregation 1 (Ospms1), all other MutL mutants from O. sativa indica subspecies displayed male and female semi-sterility similar to NIL-mlh1, but less severe than those from O. sativa japonica subspecies. MLH1 and MLH3 did not contribute in an additive fashion to fertility. Two types of MutL heterodimers, MLH1-PMS1 and MLH1-MLH3, were identified in rice, but only the latter functions in promoting meiotic CO formation. Compared to japonica varieties, indica cultivars had greater numbers of CO events per meiosis. Our results suggest that low fertility in wild rice may be caused by different gene defects, and indica and japonica subspecies have substantially different CO rates responsible for the discrepancy between the fertility of mlh1 and mlh3 mutants.
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Oryza , Proteínas de Saccharomyces cerevisiae , Animales , Oryza/genética , Retroelementos/genética , Saccharomyces cerevisiae/genética , Semillas/genética , Meiosis/genética , Mamíferos/genética , Proteínas MutL/genética , Proteínas de Saccharomyces cerevisiae/genéticaRESUMEN
Increasing grain yield is a long-term goal in crop breeding to meet the demand for global food security. Heterosis, when a hybrid shows higher performance for a trait than both parents, offers an important strategy for crop breeding. To examine the genetic basis of heterosis for yield in rice, here we generate, sequence and record the phenotypes of 10,074 F2 lines from 17 representative hybrid rice crosses. We classify modern hybrid rice varieties into three groups, representing different hybrid breeding systems. Although we do not find any heterosis-associated loci shared across all lines, within each group, a small number of genomic loci from female parents explain a large proportion of the yield advantage of hybrids over their male parents. For some of these loci, we find support for partial dominance of heterozygous locus for yield-related traits and better-parent heterosis for overall performance when all of the grain-yield traits are considered together. These results inform on the genomic architecture of heterosis and rice hybrid breeding.
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Genes de Plantas/genética , Genoma de Planta , Vigor Híbrido/genética , Oryza/crecimiento & desarrollo , Oryza/genética , Sitios de Carácter Cuantitativo/genética , Alelos , Cruzamientos Genéticos , Genes Dominantes , Genómica , Genotipo , Heterocigoto , Hibridación Genética , Oryza/anatomía & histología , Fenotipo , Fitomejoramiento , Análisis de Secuencia de ADNRESUMEN
The ankle joint is one of the important joints of the human body to maintain the ability to walk. Diseases such as stroke and ankle osteoarthritis could weaken the body's ability to control joints, causing people's gait to be out of balance. Ankle-foot orthoses can assist users with neuro/muscular or ankle injuries to restore their natural gait. Currently, passive ankle-foot orthoses are mostly designed to fix the ankle joint and provide support for walking. With the development of materials, sensing, and control science, semi-active orthoses that release mechanical energy to assist walking when needed and can store the energy generated by body movement in elastic units, as well as active ankle-foot orthoses that use external energy to transmit enhanced torque to the ankle, have received increasing attention. This article reviews the development process of ankle-foot orthoses and proposes that the integration of new ankle-foot orthoses with rehabilitation technologies such as monitoring or myoelectric stimulation will play an important role in reducing the walking energy consumption of patients in the study of human-in-the-loop models and promoting neuro/muscular rehabilitation.
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Ortesis del Pié , Tobillo , Articulación del Tobillo , Fenómenos Biomecánicos , Marcha/fisiología , Humanos , Investigación , Caminata/fisiologíaRESUMEN
Sophorae tonkinensis Radix et Rhizoma (STR) is a traditional Chinese herbal medicine. STR can reduce aminotransferase activity; however, the specific mechanism remains unclear. Here, we explored the potential therapeutic effects and hepatoprotective mechanism of STR on liver damage in mice. The chemical characteristics of the extract were characterized using ultra-high-performance liquid chromatography-tandem mass spectrometry fingerprinting, and its antioxidant capacity was verified using free radical scavenging tests. Forty-eight Kunming mice were randomly assigned into six groups. The model was made after the corresponding drug was given. The results showed that the STR water extract pretreatment significantly reduced serum aminotransferase and related liver function indicators compared with that in the model group. Furthermore, the STR water extract pretreatment significantly inhibited the apoptosis of liver cells, the level of liver high-mobility group box 1 (HMGB1), and inflammatory factors in hepatic tissue compared with that in the model group, and significantly downregulated the levels of toll-like receptor 4 (TLR4), Myeloid differentiation factor 88 (MyD88), and nuclear factor kappa B (NF-κB) compared with those in the model group. Overall, the STR water extract exerted a significant protective effect on CCL4-induced acute liver injury in this study, and the accurate active ingredients of the STR water extract will be explored in the near future.
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Enfermedad Hepática Inducida por Sustancias y Drogas , Medicamentos Herbarios Chinos , Sophora , Ratones , Animales , Medicamentos Herbarios Chinos/farmacología , Medicamentos Herbarios Chinos/química , Tetracloruro de Carbono/toxicidad , Sophora/química , Hígado , Transaminasas , Enfermedad Hepática Inducida por Sustancias y Drogas/tratamiento farmacológico , Enfermedad Hepática Inducida por Sustancias y Drogas/prevención & controlRESUMEN
Heterosis, or hybrid vigour, is a predominant phenomenon in plant genetics, serving as the basis of crop hybrid breeding, but the causative loci and genes underlying heterosis remain unclear in many crops. Here, we present a large-scale genetic analysis using 5360 offsprings from three elite maize hybrids, which identifies 628 loci underlying 19 yield-related traits with relatively high mapping resolutions. Heterotic pattern investigations of the 628 loci show that numerous loci, mostly with complete-incomplete dominance (the major one) or overdominance effects (the secondary one) for heterozygous genotypes and nearly equal proportion of advantageous alleles from both parental lines, are the major causes of strong heterosis in these hybrids. Follow-up studies for 17 heterotic loci in an independent experiment using 2225 F2 individuals suggest most heterotic effects are roughly stable between environments with a small variation. Candidate gene analysis for one major heterotic locus (ub3) in maize implies that there may exist some common genes contributing to crop heterosis. These results provide a community resource for genetics studies in maize and new implications for heterosis in plants.
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Sitios Genéticos , Vigor Híbrido , Zea mays/genética , Alelos , Genoma de Planta , Heterocigoto , FenotipoRESUMEN
Grain size is one of the key determinants of grain yield. Although a number of genes that control grain size in rice (Oryza sativa) have been identified, the overall regulatory networks behind this process remain poorly understood. Here, we report the map-based cloning and functional characterization of the quantitative trait locus GL6, which encodes a plant-specific plant AT-rich sequence- and zinc-binding transcription factor that regulates rice grain length and spikelet number. GL6 positively controls grain length by promoting cell proliferation in young panicles and grains. The null gl6 mutant possesses short grains, whereas overexpression of GL6 results in large grains and decreased grain number per panicle. We demonstrate that GL6 participates in RNA polymerase III transcription machinery by interacting with RNA polymerase III subunit C53 and transcription factor class C1 to regulate the expression of genes involved in rice grain development. Our findings reveal a further player involved in the regulation of rice grain size that may be exploited in future rice breeding.
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Grano Comestible/crecimiento & desarrollo , Grano Comestible/metabolismo , Oryza/crecimiento & desarrollo , Oryza/metabolismo , Factores de Transcripción/metabolismo , Alelos , Grano Comestible/genética , Regulación de la Expresión Génica de las Plantas/genética , Regulación de la Expresión Génica de las Plantas/fisiología , Oryza/genética , Proteínas de Plantas/genética , Proteínas de Plantas/metabolismo , Plantas Modificadas Genéticamente/genética , Plantas Modificadas Genéticamente/crecimiento & desarrollo , Plantas Modificadas Genéticamente/metabolismo , Sitios de Carácter Cuantitativo/genética , ARN Polimerasa III/genética , ARN Polimerasa III/metabolismo , Factores de Transcripción/genéticaRESUMEN
Ovarian cancer resistance to available medicines is a huge challenge in dire need of a solution, which makes its recurrence and mortality rate further exacerbated. A promising approach to overcome chemoresistance is drug screening from natural products. Here, we report that NK007, a (±)-tylophorine malate isolated from the Asclepiadaceae family, selectively inhibited the proliferation of A2780 and A2780 (Taxol) cells and migration of paclitaxel-sensitive and -resistant ovarian cancer cells. Interestingly, the decline of cell viability, including cell multiplication, clonality, and migration capacity was independent on cell apoptosis. At the molecular level, NK007 considerably induced G1/S arrest and upregulated the expression of phospho-p38 mitogen-activated protein kinase (p-p38MAPK). In addition, hexokinase 2 (HK2) protein degradation was considerably elevated in the presence of NK007, which resulted in the reduction of oxygen consumption rate and extracellular acidification rate. Altogether, our results indicate that NK007, an analog of tylophorine, can overcome paclitaxel (PTX) resistance through p38MAPK activation and HK2 degradation. As an effective, alternative antiresistance agent, NK007 exhibits a promising potential to treat PTX-resistant ovarian cancer.
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Crop domestications are long-term selection experiments that have greatly advanced human civilization. The domestication of cultivated rice (Oryza sativa L.) ranks as one of the most important developments in history. However, its origins and domestication processes are controversial and have long been debated. Here we generate genome sequences from 446 geographically diverse accessions of the wild rice species Oryza rufipogon, the immediate ancestral progenitor of cultivated rice, and from 1,083 cultivated indica and japonica varieties to construct a comprehensive map of rice genome variation. In the search for signatures of selection, we identify 55 selective sweeps that have occurred during domestication. In-depth analyses of the domestication sweeps and genome-wide patterns reveal that Oryza sativa japonica rice was first domesticated from a specific population of O. rufipogon around the middle area of the Pearl River in southern China, and that Oryza sativa indica rice was subsequently developed from crosses between japonica rice and local wild rice as the initial cultivars spread into South East and South Asia. The domestication-associated traits are analysed through high-resolution genetic mapping. This study provides an important resource for rice breeding and an effective genomics approach for crop domestication research.
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Agricultura/historia , Productos Agrícolas/genética , Evolución Molecular , Variación Genética/genética , Genoma de Planta/genética , Mapeo Geográfico , Oryza/genética , Cruzamiento/historia , Productos Agrícolas/clasificación , Productos Agrícolas/crecimiento & desarrollo , Genómica , Historia Antigua , Oryza/clasificación , Oryza/crecimiento & desarrollo , Filogenia , Polimorfismo de Nucleótido Simple/genética , Selección GenéticaRESUMEN
To systematically review the literature to identify the impact of rheumatoid arthritis (RA) on specific female sexual function domains. A meta-analysis was performed and the related literature were searched in MEDLINE, EMBASE, Cochrane Library, CNKI, CBM and Web of Science databases, and in reference lists of articles and systematic reviews. Score of the Female Sexual Function Index (FSFI) was used as the outcome measurement, and mean differences (MD) with 95% confidence intervals (CI) were calculated. Five studies were included, including 346 women with RA and 237 healthy female controls. Each domain of the FSFI score: lubrication (MD, -2.48; 95% CI, -3.69, -1.28), orgasm-1.71 (-2.09, -1.33), sexual desire-1.27 (-1.59, -0.95), satisfaction-1.67 (-2.18, -1.16), arousal-1.83 (-2.85, -0.82), pain-1.57 (-2.43, -0.70) and the total score -8.84 (-11.88, -5.79) were lower in RA women than healthy controls. Furthermore, lubrication dimension was most severely affected especially. This meta-analysis showed that female RA patients scored lower in each dimension of FSFI, mostly in the lubrication domain. It demonstrated that targeted interventions should be done to improve their sexual function. Future well-designed researches with larger sample sizes are necessary to evaluate the potential risk factors which determine female sexual dysfunction.
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Nivel de Alerta , Artritis Reumatoide/psicología , Orgasmo , Adulto , Artritis Reumatoide/fisiopatología , Femenino , Humanos , Persona de Mediana Edad , Satisfacción Personal , Factores de Riesgo , Encuestas y CuestionariosRESUMEN
Various stable circular RNAs (circRNAs) are newly identified to be the abundance of noncoding RNAs in Archaea, Caenorhabditis elegans, mice, and humans through high-throughput deep sequencing coupled with analysis of massive transcriptional data. CircRNAs play important roles in miRNA function and transcriptional controlling by acting as competing endogenous RNAs or positive regulators on their parent coding genes. However, little is known regarding circRNAs in plants. Here, we report 2354 rice circRNAs that were identified through deep sequencing and computational analysis of ssRNA-seq data. Among them, 1356 are exonic circRNAs. Some circRNAs exhibit tissue-specific expression. Rice circRNAs have a considerable number of isoforms, including alternative backsplicing and alternative splicing circularization patterns. Parental genes with multiple exons are preferentially circularized. Only 484 circRNAs have backsplices derived from known splice sites. In addition, only 92 circRNAs were found to be enriched for miniature inverted-repeat transposable elements (MITEs) in flanking sequences or to be complementary to at least 18-bp flanking intronic sequences, indicating that there are some other production mechanisms in addition to direct backsplicing in rice. Rice circRNAs have no significant enrichment for miRNA target sites. A transgenic study showed that overexpression of a circRNA construct could reduce the expression level of its parental gene in transgenic plants compared with empty-vector control plants. This suggested that circRNA and its linear form might act as a negative regulator of its parental gene. Overall, these analyses reveal the prevalence of circRNAs in rice and provide new biological insights into rice circRNAs.
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Oryza/genética , ARN de Planta/genética , Transcriptoma , Secuencia de Bases , Datos de Secuencia Molecular , Conformación de Ácido Nucleico , Oryza/metabolismo , ARN de Planta/metabolismo , Análisis de Secuencia de ARNRESUMEN
Pulse rate is one of the major physiological parameters for monitoring of cardiovascular conditions or excise states during daily life. However it is difficult to precisely measure the exact pulse rates as photoplethysmography (PPG) is easy to be affected by motion artifacts. Instead of using accelerometers followed by algorithms such as least mean square (LMS), recursive least square (RLS) and independent component analysis (ICA) or other equipment such as complex laser systems to measure displacement directly, a novel motion artifact estimation method which had lower computational complexity and higher signal dynamic range was studied and implemented, where a differential channel following green and red light PPG channels was applied to reduce the motion artifact caused by displacement of light emitting diode (LED), photo diode (PD) and tissue deformation before the analog signal was converted to digital form. A miniaturized, battery powered wrist worn artifact-resistive pulse rates monitoring system (PRMS) was presented to verify the proposed method. Four kinds of motions were performed and the results showed that the differential channel improved the morphology of the PPG signal and appeared to be artifact resistive during motions through light intensity control and high gain-phase consistency circuit design here.
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Frecuencia Cardíaca/fisiología , Monitoreo Ambulatorio/instrumentación , Fotopletismografía/instrumentación , Procesamiento de Señales Asistido por Computador , Muñeca , Algoritmos , Humanos , Modelos Teóricos , Reproducibilidad de los ResultadosRESUMEN
BACKGROUND: Physiological parameter monitoring based on photoplethysmography (PPG) detection has the advantage of fast, portable, and non-invasive. Changes in the morphology of the PPG waveform can reflect the effect of arterial elasticity changes on blood pressure (BP). However, machine learning models and non-recurrent neural network models typically ignore the time-dependency of continuous PPG data, leading to the decrease of accuracy or the increased calibration frequency. OBJECTIVE: This paper proposes a BiGRU model with attention to estimate BP trends, which uses a single-channel PPG signal combined with demographic information to estimate continuous BP trends point-by-point and to discuss the impact of calibration cycle. METHODS: This paper selects 15 typical subjects from two groups with/without cardiovascular disease (CVD) and evaluates the model performance. Regarding the calibration frequency problem, we set two modes of non-calibration and calibration to validate the results of blood pressure trends estimation. RESULTS: In the calibration mode, the estimation errors (ME ± STD) of SBP for CVD/non-CVD groups are 0.91 ± 10.58 mmHg/0.17 ± 10.06 mmHg respectively, and DBP are 0.34 ± 5.28 mmHg/-0.19 ± 5.36 mmHg; in the non-calibration mode, the estimation errors of SBP for CVD/non-CVD groups are 0.27 ± 9.87 mmHg/-0.82 ± 9.92 mmHg respectively, and DBP are -0.63 ± 3.28 mmHg/0.80 ± 4.93 mmHg. CONCLUSIONS: The results show that the proposed model has high accuracy in estimating BP levels, which is expected to achieve real-time, long-term continuous BP trends monitoring in wearable devices.
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Determinación de la Presión Sanguínea , Enfermedades Cardiovasculares , Humanos , Presión Sanguínea/fisiología , Determinación de la Presión Sanguínea/métodos , Fotopletismografía/métodos , Análisis de la Onda del PulsoRESUMEN
Surgeons and anesthesia clinicians commonly face a hemodynamic disturbance known as intraoperative hypotension (IOH), which has been linked to more severe postoperative outcomes and increases mortality rates. Increased occurrence of IOH has been positively associated with mortality and incidence of myocardial infarction, stroke, and organ dysfunction hypertension. Hence, early detection and recognition of IOH is meaningful for perioperative management. Currently, when hypotension occurs, clinicians use vasopressor or fluid therapy to intervene as IOH develops but interventions should be taken before hypotension occurs; therefore, the Hypotension Prediction Index (HPI) method can be used to help clinicians further react to the IOH process. This literature review evaluates the HPI method, which can reliably predict hypotension several minutes before a hypotensive event and is beneficial for patients' outcomes.
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Anestesia , Hipotensión , Infarto del Miocardio , Humanos , Complicaciones Intraoperatorias/diagnóstico , Complicaciones Intraoperatorias/epidemiología , Hipotensión/prevención & control , Cuidados CríticosRESUMEN
OBJECTIVE: Accurate prediction of unmearsured muscle excitations can reduce the required wearable surface electromyography (sEMG) sensors, which is a critical factor in the study of physiological measurement. Synergy extrapolation uses synergy excitations as building blocks to reconstruct muscle excitations. However, the practical application of synergy extrapolation is still limited as the extrapolation process utilizes unmeasured muscle excitations it seeks to reconstruct. This paper aims to propose and derive methods to provide an avenue for the practical application of synergy extrapolation with non-negative matrix factorization (NMF) methods. APPROACH: Specifically, a tunable Gaussian-Laplacian mixture distribution NMF (GLD-NMF) method and related multiplicative update rules are derived to yield appropriate synergy excitations for extrapolation. Furthermore, a template-based extrapolation structure (TBES) is proposed to extrapolate unmeasured muscle excitations based on synergy weighting matrix templates totally extracted from measured sEMG datasets, improving the extrapolation performance. Moreover, we applied the proposed GLD-NMF method and TBES to selected muscle excitations acquired from a series of single-leg stance (SLS) tests, walking tests and upper limb reaching tests. MAIN RESULTS: Experimental results show that the proposed GLD-NMF and TBES could extrapolate unmeasured muscle excitations accurately. Moreover, introducing synergy weighting matrix templates could decrease the number of sEMG sensors in a series of experiments. In addition, verification results demonstrate the feasibility of applying synergy extrapolation with NMF methods. SIGNIFICANCE: With the TBES method, synergy extrapolation could play a significant role in reducing data dimensions of sEMG sensors, which will improve the portability of sEMG sensors-based systems and promotes applications of sEMG signals in human-machine interfaces scenarios.
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There is limited data on the prognostic implications of residual mild coarctation (RMC) in patients with repaired native coarctation of the aorta (CoA). To explore the association of RMC with mid-term comorbidities in post-interventional patients, and the predictive value of the residual pressure gradient. The authors retrospectively analyzed 79 native CoA patients who received successful intervention at our hospital between October 2010 and June 2023. The outcomes of the study were late arterial hypertension (either raised blood pressure or commencement of hypotensive medications) only in normotensive patients at early follow-up and the composite mid-term comorbidities including new-onset aortic injury, re-stenosis, and re-intervention. At a median follow-up of 60 months, late hypertension and mid-term comorbidities occurred in 16 (28.1%) and nine (11.4%) patients, respectively. Multivariate Cox proportional hazard regression analysis identified invasive peak systolic CoA pressure gradient (PSPG) as the best independent predictor of both outcomes. The maximally selected rank statistics indicated 10 mm Hg as the best PSPG cut-off value for predicting late hypertension. Compared to patients with PSPG < 11 mm Hg, the cumulative event rates of both outcomes were higher in those with PSPG ≥ 11 mm Hg (log-rank test, p < .001 for both endpoints). PSPG ≥ 11 mm Hg was proved to be the independent predictor of late hypertension with a significantly increased risk. In patients with non-surgical CoA repair, the post-interventional RMC and PSPG ≥11 mm Hg are important predictors of clinical comorbidities at mid-term follow-up.
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Introduction: Rice (Oryza sativa) serves as a vital staple crop that feeds over half the world's population. Optimizing rice breeding for increasing grain yield is critical for global food security. Heading-date-related or Flowering-time-related traits, is a key factor determining yield potential. However, traditional manual phenotyping methods for these traits are time-consuming and labor-intensive. Method: Here we show that aerial imagery from unmanned aerial vehicles (UAVs), when combined with deep learning-based panicle detection, enables high-throughput phenotyping of heading-date-related traits. We systematically evaluated various state-of-the-art object detectors on rice panicle counting and identified YOLOv8-X as the optimal detector. Results: Applying YOLOv8-X to UAV time-series images of 294 rice recombinant inbred lines (RILs) allowed accurate quantification of six heading-date-related traits. Utilizing these phenotypes, we identified quantitative trait loci (QTL), including verified loci and novel loci, associated with heading date. Discussion: Our optimized UAV phenotyping and computer vision pipeline may facilitate scalable molecular identification of heading-date-related genes and guide enhancements in rice yield and adaptation.
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The genome's dynamic nature, exemplified by elements like extrachromosomal circular DNA (eccDNA), is crucial for biodiversity and adaptation. Yet, the role of eccDNA in plants, particularly rice, remains underexplored. Here, we identify 25,598 eccDNAs, unveiling the widespread presence of eccDNA across six rice tissues and revealing its formation as a universal and random process. Interestingly, we discover that direct repeats play a pivotal role in eccDNA formation, pointing to a unique origin mechanism. Despite eccDNA's prevalence in coding sequences, its impact on gene expression is minimal, implying its roles beyond gene regulation. We also observe the association between eccDNA's formation and minor chromosomal deletions, providing insights of its possible function in regulating genome stability. Further, we discover eccDNA specifically accumulated in rice leaves, which may be associated with DNA damage caused by environmental stressors like intense light. In summary, our research advances understanding of eccDNA's role in the genomic architecture and offers valuable insights for rice cultivation and breeding.
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Oryza , Oryza/genética , ADN Circular/genética , Fitomejoramiento , ADN , GenomaRESUMEN
Introduction: Human-in-the-loop optimization has made great progress to improve the performance of wearable robotic devices and become an effective customized assistance strategy. However, a lengthy period (several hours) of continuous walking for iterative optimization for each individual makes it less practical, especially for disabled people, who may not endure this process. Methods: In this paper, we provide a muscle-activity-based human-in-the-loop optimization strategy that can reduce the time spent on collecting biosignals during each iteration from around 120 s to 25 s. Both Bayesian and Covariance Matrix Adaptive Evolution Strategy (CMA-ES) optimization algorithms were adopted on a portable hip exoskeleton to generate optimal assist torque patterns, optimizing rectus femoris muscle activity. Four volunteers were recruited for exoskeleton-assisted walking trials. Results and Discussion: As a result, using human-in-the-loop optimization led to muscle activity reduction of 33.56% and 41.81% at most when compared to walking without and with the hip exoskeleton, respectively. Furthermore, the results of human-in-the-loop optimization indicate that three out of four participants achieved superior outcomes compared to the predefined assistance patterns. Interestingly, during the optimization stage, the order of the two typical optimizers, i.e., Bayesian and CMA-ES, did not affect the optimization results. The results of the experiment have confirmed that the assistance pattern generated by muscle-activity-based human-in-the-loop strategy is superior to predefined assistance patterns, and this strategy can be achieved more rapidly than the one based on metabolic cost.