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1.
Anal Chem ; 90(10): 6089-6095, 2018 05 15.
Artículo en Inglés | MEDLINE | ID: mdl-29672026

RESUMEN

We describe here a flow platform for quantifying the number of biomolecules on individual fluorescent nanoparticles. The platform combines line-confocal fluorescence detection with near nanoscale channels (1-2 µm in width and height) to achieve high single-molecule detection sensitivity and throughput. The number of biomolecules present on each nanoparticle was determined by deconvolving the fluorescence intensity distribution of single-nanoparticle-biomolecule complexes with the intensity distribution of single biomolecules. We demonstrate this approach by quantifying the number of streptavidins on individual semiconducting polymer dots (Pdots); streptavidin was rendered fluorescent using biotin-Alexa647. This flow platform has high-throughput (hundreds to thousands of nanoparticles detected per second) and requires minute amounts of sample (∼5 µL at a dilute concentration of 10 pM). This measurement method is an additional tool for characterizing synthetic or biological nanoparticles.


Asunto(s)
Nanopartículas/química , Polímeros/química , Estreptavidina/análisis , Biotina/química , Fluorescencia , Colorantes Fluorescentes/química , Técnicas Analíticas Microfluídicas , Microscopía Confocal , Semiconductores
2.
Biomed Environ Sci ; 31(9): 667-676, 2018 09.
Artículo en Inglés | MEDLINE | ID: mdl-30369345

RESUMEN

OBJECTIVE: To investigate the effect of fermented barley extracts with Lactobacillus plantarum dy-1 (LFBE) for modulating glucose consumption in HepG2 cells via miR-212 regulation. METHODS: Hepatocellular carcinoma (HepG2) cells were treated with palmitate. After 12 h, palmitate-induced HepG2 cells were treated with LFBE and its main components. Changes in glucose consumption, proinflammatory cytokine secretion, and miRNA-212 expression in HepG2 cells was observed. RESULTS: Treatment with LFBE rich in vanillic acid (VA) increased glucose consumption and reduced proinflammatory cytokine secretion in HepG2 cells. LFBE and VA normalized the upregulation of miR-212, which led to the upregulation of dual-specificity phosphatase-9 (DUSP9), a direct target of miR-212, at both protein and mRNA levels. Downregulation of miR-212 markedly increased glucose consumption and reduced proinflammatory cytokine secretion by enhancing DUSP9 expression. CONCLUSION: The results showed the benefit of LFBE and miR-212 downregulation in modulating glucose consumption and reducing proinflammatory cytokine secretion by targeting DUSP9. VA in LFBE was a strong regulator of palmitate-induced abnormal glucose consumption in HepG2 cells and can be a primary mediator.


Asunto(s)
Glucosa/metabolismo , Hordeum/química , Lactobacillus plantarum/química , Extractos Vegetales/química , Ácido Vanílico/análisis , Regulación hacia Abajo , Fermentación , Células Hep G2 , Humanos , MicroARNs/metabolismo , Regulación hacia Arriba
3.
Biomed Environ Sci ; 30(1): 10-21, 2017 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-28245895

RESUMEN

OBJECTIVE: A subcutaneous transplantation tumor model of human HT-29 cells was established in nude mice to study the anticarcinogenic activities and apoptosis-regulatory mechanistic effect of aqueous extract of fermented barley with Lactobacillus plantarum dy-1 (LFBE). METHODS: HT-29 cells were transplanted via subcutaneous injection of 1 × 107cells into the right flank of each nude mouse. Then, nude mice were treated for 30 days with LFBE (high-dose 2 g·kg-1·d-1; low-dose 1 g·kg-1·d-1) and for 7 days with 5-fluorouracil (5-FU, 25 g·kg-1·d-1) by gavage and intraperitoneal injection, respectively. RESULTS: Tumor volume and weight decreased significantly in both groups of nude mice treated with LFBE. In addition, the cell apoptosis rate of the LFBE group was significantly higher than that of the control group and 5-FU groups as measured by the TUNEL assay. Moreover, the real-time fluorescent quantitative PCR and Western blot methods further confirmed these apoptosis-enhancing and growth-inhibiting effects. The involvement of LFBE in inducing apoptosis was confirmed by the expression of Bax, Bcl-2, caspase-3, and cyclinD1. CONCLUSION: The results showed that LFBE could induce subcutaneous transplantation tumor apoptosis in nude mice and could be used as a natural nutrient supplement or chemopreventive agent in the treatment of human colon cancer.


Asunto(s)
Apoptosis/efectos de los fármacos , Hordeum/química , Neoplasias Experimentales/tratamiento farmacológico , Fitoterapia , Extractos Vegetales/uso terapéutico , Animales , Caspasa 3/metabolismo , Proliferación Celular/efectos de los fármacos , Ciclina D1/metabolismo , Femenino , Fermentación , Células HT29 , Humanos , Lactobacillus plantarum , Ratones Desnudos , Trasplante de Neoplasias , Neoplasias Experimentales/metabolismo , Extractos Vegetales/farmacología , Ensayos Antitumor por Modelo de Xenoinjerto , Proteína X Asociada a bcl-2/metabolismo
4.
J Am Chem Soc ; 137(1): 173-8, 2015 Jan 14.
Artículo en Inglés | MEDLINE | ID: mdl-25494172

RESUMEN

This article describes the design and development of squaraine-based semiconducting polymer dots (Pdots) that show large Stokes shifts and narrow-band emissions in the near-infrared (NIR) region. Fluorescent copolymers containing fluorene and squaraine units were synthesized and used as precursors for preparing the Pdots, where exciton diffusion and likely through-bond energy transfer led to highly bright and narrow-band NIR emissions. The resulting Pdots exhibit the emission full width at half-maximum of ∼36 nm, which is ∼2 times narrower than those of inorganic quantum dots in the same wavelength region (∼66 nm for Qdot705). The squaraine-based Pdots show a high fluorescence quantum yield (QY) of 0.30 and a large Stokes shift of ∼340 nm. Single-particle analysis indicates that the average per-particle brightness of the Pdots is ∼6 times higher than that of Qdot705. We demonstrate bioconjugation of the squaraine Pdots and employ the Pdot bioconjugates in flow cytometry and cellular imaging applications. Our results suggest that the narrow bandwidth, high QY, and large Stokes shift are promising for multiplexed biological detections.


Asunto(s)
Ciclobutanos/química , Fluorescencia , Neoplasias/patología , Fenoles/química , Polímeros/química , Puntos Cuánticos , Ciclobutanos/síntesis química , Citometría de Flujo , Humanos , Rayos Infrarrojos , Células MCF-7 , Estructura Molecular , Tamaño de la Partícula , Fenoles/síntesis química , Polímeros/síntesis química , Semiconductores , Propiedades de Superficie
5.
Biomed Environ Sci ; 28(10): 718-27, 2015 Oct.
Artículo en Inglés | MEDLINE | ID: mdl-26582094

RESUMEN

OBJECTIVE: A subcutaneous transplantation tumor model of human HT-29 cells in nude mice was established to evaluate anticarcinogenic activities, and the apoptosis-regulated mechanism effect of aqueous extract of fermented wheat germ with Lactobacillus plantarum dy-1 (LFWGE). METHODS: The HT-29 cells were transplanted via subcutaneous injection of 1×107 cells into the right flank of each nude mouse. Then, nude mice were treated for 30 d with LFWGE (high-dose 2 g/kg/d; low-dose 1 g/kg/d) and for 7 d with 5-fluorouracil (5-FU, 25 mg/kg/d) by gavage and intraperitoneal injection, respectively. An inhibition of tumor growth was observed. RESULTS: Tumor volume and weights decreased significantly in both groups of nude mice treated with LFWGE. In addition, the cell apoptosis rate of the LFWGE group (2 g/kg/d, 60.1%±4.4%; 1 g/kg/d, 58.6%±6.9%) was significantly higher than that of the control group (11.5%±1.6%) and 5-FU group (32.1%±3.5%) as measured by the TUNEL assay. Moreover, the real-time fluorescent quantitative PCR and Western blot method further confirmed these enhancing apoptosis and growth inhibition effects. The involvement of LFWGE in inducing apoptosis was confirmed by the expression of Bax, Bcl-2, Caspase-3, and CyclinD1. CONCLUSION: The results showed that LFWGE could induce subcutaneous transplantation tumor apoptosis in nude mice and could be as a natural nutrient supplements or chemopreventive agent in the treatment of human colon cancer.


Asunto(s)
Antineoplásicos/farmacología , Apoptosis/efectos de los fármacos , Neoplasias Experimentales/tratamiento farmacológico , Extractos Vegetales/farmacología , Animales , Antineoplásicos/química , Caspasa 3/genética , Caspasa 3/metabolismo , Ciclina D1/genética , Ciclina D1/metabolismo , Regulación Neoplásica de la Expresión Génica/efectos de los fármacos , Células HT29 , Humanos , Ratones , Ratones Desnudos , Extractos Vegetales/química , Proteínas Proto-Oncogénicas c-bcl-2/genética , Proteínas Proto-Oncogénicas c-bcl-2/metabolismo , Proteína X Asociada a bcl-2/genética , Proteína X Asociada a bcl-2/metabolismo
7.
Oncol Lett ; 22(4): 726, 2021 Oct.
Artículo en Inglés | MEDLINE | ID: mdl-34429766

RESUMEN

Endoplasmic reticulum stress (ERS) and autophagy are important pathways, which induce apoptosis of tumor cells. Osthole has been demonstrated to exert anticancer effects via the induction of apoptosis in several human colon cancer lines, but the mechanism underlying its involvement in the induction of ERS and autophagy in the human HT-29 colorectal cancer cell line remains unknown. The present study aimed to identify the possible signaling pathways involved in osthole-induced apoptosis of HT29 cells. Methodologically, colony formation and Cell Counting Kit-8 assays were used to assess cell proliferation and viability, respectively, while flow cytometry was performed to investigate apoptosis. Signaling pathways, including apoptosis, autophagy and ERS, were also investigated in the HT-29 cell line using western blot analysis. The results demonstrated that osthole inhibited cellular proliferation and viability in a dose-dependent manner. In addition, osthole induced the expression level of proteins associated with mitochondria-mediated cell apoptosis, autophagy and ERS. The association between autophagy and ERS in osthole-induced apoptosis in the HT-29 cell line was further clarified. Inhibiting cell autophagy with the inhibitor, 3-methyladenine, suppressed osthole-induced cell apoptosis and enhanced osthole-induced ERS. By contrast, alleviating ERS with the inhibitor, 4-phenylbutyric acid attenuated osthole-induced cell apoptosis and autophagy. In conclusion, osthole could significantly suppress the proliferation and viability of the HT-29 colorectal cancer cell line and induce cell apoptosis via autophagy and ERS. Furthermore, ERS may play a more important role in osthole-induced cell apoptosis.

8.
Food Chem ; 311: 126026, 2020 May 01.
Artículo en Inglés | MEDLINE | ID: mdl-31869641

RESUMEN

The enrichment of ß-glucan in barley significantly decreases the quality of dough and baked bread, and ß-glucanase can improve dough and bread quality. Nevertheless, the mechanism by which ß-glucanase improves the quality of fermented barley flour-based products is still poorly understood. The gluten microcosmic structure, molecular structure and yeast gas production capacity were investigated using Fourier transform infrared (FT-IR) spectroscopy, Raman spectroscopy, Scanning electron microscopy (SEM) and F3 rheological fermentation techniques. The results showed that ß-Glucanase can degrade the high-molecular-weight ß-glucan to low-molecular-weight oligosaccharide fragments, which reduces the viscosity of the ß-glucans, promotes cross-linking between the gluten molecules, and indirectly improves the gluten network structure, thereby alleviating the negative effects of ß-glucans. The ß-glucosidase produced during yeast metabolism can further hydrolyse low-molecular-weight oligosaccharides into reducing sugars that can be used by yeast, increasing the carbon sources available to yeasts and the gas production capability of yeasts.


Asunto(s)
Manipulación de Alimentos , Glútenes/química , Glicósido Hidrolasas/metabolismo , Hordeum/química , beta-Glucanos/química , Pan/normas , Fermentación , Harina/normas , Hidrólisis , Microscopía Electrónica de Rastreo , Peso Molecular , Reología , Espectroscopía Infrarroja por Transformada de Fourier , Espectrometría Raman , Viscosidad
9.
Zhongguo Yi Xue Ke Xue Yuan Xue Bao ; 31(4): 432-7, 2009 Aug.
Artículo en Zh | MEDLINE | ID: mdl-19771729

RESUMEN

OBJECTIVE: To explore the influences of intervention on the abilities of detecting pulmonary tuberculosis cases in general hospitals. METHODS: We selected 6 general hospitals at 3 different levels (A, B, and C). The intervened group included hospitals A1, B1, and C1, and the non-intervened group included hospitals A2, B2, and C2. The results after intervention were compared. RESULTS: The report rate of pulmonary tuberculosis, sputum positive rate of reported cases, and sputum check rate of reported cases were significantly higher in hospital A1 than grouping hospital A2 (P = 0.000, P = 0.045, and P = 0.017, respectively). The report rate and sputum examination rate of reported cases were significantly higher in hospital B1 than grouping hospital B2 (P = 0.000, P = 0.024, respectively). The report rate and sputum examination rate of reported cases were significantly lower in hospital C1 than grouping hospital C2 (P = 0.000, P = 0.001, respectively). In hospital A1, the report rate, sputum positive rate of reported cases, and sputum check rate of reported cases were not significantly different before and after intervention (P = 0.182, P = 0.116, and P = 0.583, respectively). In hospital B1, the report rate were significantly different before and after intervention (P = 0.004), while the sputum positive rate of reported cases and sputum check rate of reported cases were not significantly different (P = 0.909, P = 0.052, respectively). In hospital C1, the report rate was significantly higher after intervention (P = 0.025). In hospital C2, the sputum check rate significantly increased (P = 0.000). CONCLUSIONS: Intervention influences the hospitals abilities to detect pulmonary tuberculosis cases. However, more optimized and long-term intervention mechanism should be established to increase case detection rate of pulmonary tuberculosis.


Asunto(s)
Hospitales Generales , Tuberculosis Pulmonar/diagnóstico , Humanos , Esputo/microbiología
10.
Biomed Environ Sci ; 32(10): 755-768, 2019 Oct.
Artículo en Inglés | MEDLINE | ID: mdl-31843045

RESUMEN

OBJECTIVE: To further explore associated effects of Lactobacillus plantarum dy-1 (LFBE) on obesity and lipid metabolism at the gene expression level, the expression of microRNAs (miRNAs) was investigated in the liver of high-fat diet (HFD) induced obese rats. METHODS: Three groups of animal models were established. Changes in miRNA expression in the liver of each group were analyzed by microarray and RT-qPCR, complemented by bioinformatics. Palmitateinduced hepatocellular carcinoma (HepG2) cells were used as a model to validate the test. RESULTS: LFBE treatment groups and HFD groups were observed to be distinctly different with respect to rates of increase in body weight and body fat percentage and triglyceride (TG) and total cholesterol (TC) levels in serum and liver. In addition, the LFBE group showed upregulation of ten miRNAs and downregulation of five miRNAs in the liver. Downregulation of miR-34a and miR-212 was observed in the livers of the LFBE group. Gene ontology and kyoto encyelopedia of geues and genomes (KEGG) pathway analysis showed that possible target genes of the deregulated miRNAs were significantly enriched in the adrenergic and HIF-1 signaling pathways. CONCLUSION: These results demonstrate that LFBE might regulate the expression of miRNAs in order to inhibit obesity and fatty liver.


Asunto(s)
Fármacos Antiobesidad/farmacología , Dieta Alta en Grasa , Hordeum/química , Lactobacillus plantarum/química , Obesidad/dietoterapia , Extractos Vegetales/farmacología , Animales , Fermentación , Expresión Génica/efectos de los fármacos , Hígado/metabolismo , Masculino , MicroARNs/genética , Obesidad/etiología , Ratas , Ratas Sprague-Dawley
11.
Zhonghua Yi Xue Za Zhi ; 88(48): 3397-400, 2008 Dec 30.
Artículo en Zh | MEDLINE | ID: mdl-19159568

RESUMEN

OBJECTIVE: To investigate the expression of estrogen receptor (ER), progesterone receptor (PR), and human epithelial growth factor receptor 2 (HER2) in breast cancer, and to explore their correlation with the age of patient, and size, clinic stage, and lymph node metastasis of the tumor. METHODS: The data of 910 breast cancer, 89.4% of invasive ductal carcinoma, 1.7% of invasive lobular carcinoma, 44 cases 5% of ductal carcinoma in situ, and 4.9% of other pathologic types, 29.9% being less than 2 cm, 45.6% being 2-5 cm, and 24.5% bigger than 5 cm in size, 54.2% without metastasis in lymph node, 25.5% with metastasis in 1-3 lymph nodes, and 20.3% with metastasis in more than 3 lymph nodes respectively, were analyzed retrospectively. Immunohistochemistry was used to detect the expression of ER, PR, and HER2. RESULTS: The ER negative expression rate was 33.0%, and PR negative expression rate was 27.4%, and HER2 overexpression rate was 20.3%. The possibility of lymph node metastasis decreased along with the increase of age (P < 0.001). Tumor size was negatively correlated with the expression of ER and PR (both P < 0.001), and positively correlated with the expression of HER2 (P < 0.001). There was no significant difference between the situation of lymph node metastasis and the expression of ER, PR and HER2 in primary tumors. CONCLUSION: As good prognostic markers of breast invasive ductal cancer, ER and PR are negatively correlated with the HER2 expression, as a worse prognostic marker. ER/PR positive or HER2 negative tumors are morel likely to be diagnosed at earlier stages.


Asunto(s)
Biomarcadores de Tumor/metabolismo , Neoplasias de la Mama/metabolismo , Receptor alfa de Estrógeno/metabolismo , Receptor ErbB-2/metabolismo , Receptores de Progesterona/metabolismo , Adulto , Anciano , Neoplasias de la Mama/patología , Femenino , Humanos , Inmunohistoquímica , Metástasis Linfática , Persona de Mediana Edad , Invasividad Neoplásica , Estadificación de Neoplasias , Pronóstico , Estudios Retrospectivos
12.
Org Lett ; 9(22): 4471-4, 2007 Oct 25.
Artículo en Inglés | MEDLINE | ID: mdl-17902681

RESUMEN

"Push-pull" phenyltetraene-based chromophores are too sensitive to be incorporated into Diels-Alder-type cross-linkable polymers due to the reactivity of its diene segment with maleimides. A facile synthetic route has been explored to incorporate a methoxy group into the R position of such chromophores, which reduces their diene reactivity during the poling and lattice hardening process. The poled polymers with one of such chromophores doped in a cross-linked polymer lattice showed ultrahigh electro-optic activities, up to 306 pm/V at 1310 nm.

13.
Org Lett ; 8(7): 1387-90, 2006 Mar 30.
Artículo en Inglés | MEDLINE | ID: mdl-16562898

RESUMEN

[reaction: see text] A facile synthetic route has been developed to convert an electron-rich, sterically hindered dialkylaminodienone into a conjugated dialkylaminotrienal with good yield. The derived dialkylaminotetraene-type nonlinear optical chromophores possess an all-trans conformation and can be functionalized with fluoro-dendron to provide proper shape modification for poling. Polymers doped with two examples of these chromophores in high concentrations have been poled to afford ultra-large electrooptic coefficients (r(33)) of 208 and 262 pm/V, respectively, at the measuring wavelength of 1.31 mum.

15.
J Agric Food Chem ; 63(9): 2449-57, 2015 Mar 11.
Artículo en Inglés | MEDLINE | ID: mdl-25658135

RESUMEN

This study aimed to evaluate the anticarcinogenic activities of aqueous extract of fermented wheat germ with Lactobacillus plantarum dy-1 (LFWGE). The anticarcinogenic activities, including antiproliferative effects and the induction of apoptosis, were studied in human HT-29 colon cancer cells. The 2,6-dimethoxybenzoquinone and total phenol contents in LFWGE were determined by HPLC and the Folin-Ciocalteu method. In addition, some functional proteins were separated and purified by gel filtration chromatography. There were 21 proteins identified by LC-MS/MS. The sugars isolated from LFWGE did not possess any anticarcinogenic activity. The results of an MTT assay showed high antiproliferative effects of LFWGE. In addition, LFWGE attenuated the progression from the G0-G1 to the G2-M phase of the cell cycle, and LFWGE-induced cell apoptosis was associated with the activation of caspase-3. LFWGE and its major bioactive ingredients inhibited the proliferation of HT-29 cells via apoptosis and thus may be a potential anticarcinogenic agent.


Asunto(s)
Antineoplásicos Fitogénicos/farmacología , Apoptosis/efectos de los fármacos , Proliferación Celular/efectos de los fármacos , Neoplasias del Colon/fisiopatología , Lactobacillus plantarum/metabolismo , Extractos Vegetales/farmacología , Antineoplásicos Fitogénicos/química , Antineoplásicos Fitogénicos/metabolismo , Ciclo Celular/efectos de los fármacos , Neoplasias del Colon/tratamiento farmacológico , Fermentación , Células HT29 , Humanos , Extractos Vegetales/química , Extractos Vegetales/metabolismo
17.
Org Lett ; 5(19): 3543-6, 2003 Sep 18.
Artículo en Inglés | MEDLINE | ID: mdl-12967320

RESUMEN

[structure: see text] A convenient approach to a series of star-shaped pi-conjugated organic materials with oligofluorenes (OF) as the branches and a benzene ring as the core has been developed. The optical properties of these materials are liable to be tuned by introduction of more fluorene rings. The investigation of the structure-property relationship of these materials has indicated differences between star-shaped oligofluorenes and linear oligofluorenes, as well as polyfluorenes.


Asunto(s)
Fluorenos/síntesis química , Polímeros/síntesis química , Reactivos de Enlaces Cruzados/química , Espectroscopía de Resonancia Magnética , Modelos Químicos , Espectrometría de Masa por Láser de Matriz Asistida de Ionización Desorción/métodos , Estereoisomerismo
18.
Pharmacogn Mag ; 10(Suppl 1): S92-9, 2014 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-24914315

RESUMEN

BACKGROUND: Silybum marianum has been used as herbal medicine for the treatment of liver disease, liver cirrhosis, and to prevent liver cancer in Europe and Asia since ancient times. Silybum marianum oil (SMO), a by-product of silymarin production, is rich in essential fatty acids, phospholipids, sterols, and vitamin E. However, it has not been very good development and use. OBJECTIVE: In the present study, we used olive oil as a control to investigate the antioxidant and anti-aging effect of SMO in D-galactose (D-gal)-induced aging mice. MATERIALS AND METHODS: D-gal was injected intraperitoneally (500 mg/kg body weight daily) for 7 weeks while SMO was simultaneously administered orally. The triglycerides (TRIG) and cholesterol (CHOL) levels were estimated in the serum. Superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), total antioxidant capacity (T-AOC), monoamine oxidase (MAO), malondialdehyde (MDA), caspase-3, and Bcl-2 were determined in the liver and brain. The activities of Na(+)-K(+)-adenosine triphosphatase (ATPase), Ca(2+)-Mg(2+)-ATPase, membrane potential (ΔΨm), and membrane fluidity of the liver mitochondrial were estimated. RESULTS: SMO decreased levels of TRIG and CHOL in aging mice. SMO administration elevated the activities of SOD, GSH-Px, and T-AOC, which are suppressed by aging. The levels of MAO and MDA in the liver and brain were reduced by SMO administration in aging mice. Enzyme linked immunosorbent assay showed that SMO significantly decreased the concentration of caspase-3 and improved the activity of Bcl-2 in the liver and brain of aging mice. Furthermore, SMO significantly attenuated the D-gal induced liver mitochondrial dysfunction by improving the activities of Na(+)-K(+)-ATPase, Ca(2+)-Mg(2+)-ATPase, membrane potential (ΔΨm), and membrane fluidity. CONCLUSION: These results indicate that SMO effectively attenuated oxidative damage and improved apoptosis related factors as well as liver mitochondrial dysfunction in aging mice.

19.
Food Chem Toxicol ; 74: 20-7, 2014 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-25194626

RESUMEN

The transgenic rice line (TRS) enriched with amylose and resistant starch (RS) was developed by antisense RNA inhibition of starch-branching enzymes. Cereal starch with high amylose has a great benefit on human health through its resistant starch. In order to evaluate the effect of transgenic rice on rats, the rats were fed diets containing 70% TRS rice flour, its near-isogenic rice flour or the standard diet as the control through three generations. In the present study, clinical performance, reproductive capacity and pathological responses including body weight, food consumption, reproductive data, hematological parameters, serum chemistry components, organ relative weights and histopathology were examined. Some statistically significant differences were observed in rats consuming the high amylose rice diet when compared to rats fed the near-isogenic control rice diet or the conventional (non-rice) standard diet. These differences were generally of small magnitude, appeared to be random in nature, and were within normal limits for the strain of rat used, and were therefore not considered to be biologically meaningful or treatment related.


Asunto(s)
Amilosa/metabolismo , Oryza , Reproducción/efectos de los fármacos , Amilosa/análisis , Animales , Peso Corporal/efectos de los fármacos , Dieta , Ingestión de Alimentos/efectos de los fármacos , Femenino , Alimentos Fortificados/efectos adversos , Alimentos Fortificados/análisis , Masculino , Oryza/efectos adversos , Oryza/química , Oryza/genética , Ovario/efectos de los fármacos , Ovario/patología , Plantas Modificadas Genéticamente/efectos adversos , Plantas Modificadas Genéticamente/química , Plantas Modificadas Genéticamente/genética , Ratas Sprague-Dawley , Testículo/efectos de los fármacos , Testículo/patología
20.
Food Chem Toxicol ; 50(6): 1902-10, 2012 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-22510491

RESUMEN

Lysine-rich rice (LR) is a transgenic rice produced by fusion protein expressed genes into the germline of rice seeds. Compositional analysis of LR showed that the absolute concentration of lysine was significantly higher as compared to a near-isogenic non-transgenic rice. Lysine is believed to be the first limiting essential amino acid in rice, it is important to improve lysine content on rice nutritional quality. Here we report the results of a three generation study comparing the outcome in rats fed the transgenic rice to those fed conventional, near-isogenic rice or a control diet. In the study, both clinical performance variables and pathological responses such as body weight, food consumption, reproductive data, hematological parameters, serum chemistry and relative organ weights were examined respectively. It was evident that there were no adverse effects observed in rats that were fed transgenic rice compared with non-transgenic rice. There were significant differences in some hematology, serum chemistry parameters and relative organ weights in rats consuming the transgenic rice diet or non-transgenic rice diet compared with the control diet, but no macroscopic or histological adverse effects were observed. So the results from this study demonstrate that LR rice is as safe as near-isogenic non-transgenic rice.


Asunto(s)
Lisina/farmacología , Oryza/efectos adversos , Plantas Modificadas Genéticamente/efectos adversos , Plantas Modificadas Genéticamente/química , Animales , Recuento de Células Sanguíneas , Análisis Químico de la Sangre , Peso Corporal/efectos de los fármacos , Dieta , Ingestión de Alimentos/efectos de los fármacos , Hormonas Esteroides Gonadales/sangre , Lactancia/fisiología , Masculino , Estado Nutricional , Tamaño de los Órganos/efectos de los fármacos , Oryza/química , Oryza/genética , Ratas , Ratas Sprague-Dawley , Reproducción/efectos de los fármacos , Caracteres Sexuales
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