RESUMEN
The results of investigation of the inflammatory processes dynamics in operative wounds in 238 patients after surgical intervention, performed for noncomplicated hernias of anterior abdominal wall of various localization, as well as the impact of polarized light on correction of the vegetative nervous system disorders, for prophylaxis of infiltrative-purulent complications were analyzed.
Asunto(s)
Hernia Abdominal/cirugía , Hernia Abdominal/terapia , Herniorrafia , Fototerapia/métodos , Complicaciones Posoperatorias/prevención & control , Cicatrización de Heridas/efectos de la radiación , Pared Abdominal/patología , Pared Abdominal/efectos de la radiación , Pared Abdominal/cirugía , Adulto , Anciano , Sistema Nervioso Autónomo/efectos de la radiación , Femenino , Hernia Abdominal/patología , Humanos , Masculino , Persona de Mediana Edad , Fototerapia/instrumentaciónRESUMEN
Abstract The results of investigation on dynamics of a local immunity indices in an acute appendicitis, depending on the pathological process stage as well as on bacteriological investigation of parietal microflora of processus vermicularis, were adduced. The sIgA and lisocymal dynamics have witnessed that while a destructive process progressing their concentration was enhanced, and in a gangrenous acute appendicitis they practically disappeared. Due to affection of a barrier function of the processus vermicularis wall a favorable conditions were created for the microorganisms intramural translocation as well as to abdominal cavity.
Asunto(s)
Apendicitis/inmunología , Apéndice/inmunología , Infecciones por Bacteroides/inmunología , Infecciones por Enterobacteriaceae/inmunología , Cavidad Abdominal/microbiología , Cavidad Abdominal/patología , Cavidad Abdominal/cirugía , Apendicitis/microbiología , Apendicitis/patología , Apendicitis/cirugía , Apéndice/microbiología , Apéndice/patología , Apéndice/cirugía , Traslocación Bacteriana , Bacteroides/inmunología , Bacteroides/patogenicidad , Infecciones por Bacteroides/microbiología , Infecciones por Bacteroides/patología , Infecciones por Bacteroides/cirugía , Enterobacteriaceae/inmunología , Enterobacteriaceae/patogenicidad , Infecciones por Enterobacteriaceae/microbiología , Infecciones por Enterobacteriaceae/patología , Infecciones por Enterobacteriaceae/cirugía , Humanos , Inmunidad Innata , Inmunoglobulina A/biosíntesis , Inmunoglobulina G/biosíntesis , Inmunoglobulina M/biosíntesis , Muramidasa/inmunologíaRESUMEN
Peculiarities of microflora in the appendix mucosa and abdominal exudate in different morphological forms of an acute appendicitis (AA) were studied up. In accordance to the bacteriological investigations data, anaerobic, and aerobic microorganisms in AA were revealed in a monoculture and in association, more frequently--obligate anaer- obes (bacteroids) with E. coli--in 82 (80.39%) observations, staphylococcus--in 52 (50.98%), fecal streptococcus--in 19 (18.63%). With progression of inflammatory process and destructive changes in the appendix wall the quantity of bacteroids and enterobacteria have had enhanced, while the quantity of lacto- and bifidumflora-- reduced.
Asunto(s)
Apendicitis/microbiología , Apéndice/microbiología , Infecciones por Escherichia coli/microbiología , Mucosa Intestinal/microbiología , Infecciones Estafilocócicas/microbiología , Infecciones Estreptocócicas/microbiología , Actinobacteria/crecimiento & desarrollo , Enfermedad Aguda , Apendicitis/diagnóstico , Apendicitis/patología , Apéndice/patología , Carga Bacteriana , Técnicas de Tipificación Bacteriana , Escherichia coli/crecimiento & desarrollo , Escherichia coli/patogenicidad , Infecciones por Escherichia coli/diagnóstico , Infecciones por Escherichia coli/patología , Humanos , Mucosa Intestinal/patología , Lactobacillaceae/crecimiento & desarrollo , Infecciones Estafilocócicas/diagnóstico , Infecciones Estafilocócicas/patología , Staphylococcus/crecimiento & desarrollo , Staphylococcus/patogenicidad , Infecciones Estreptocócicas/diagnóstico , Infecciones Estreptocócicas/patología , Streptococcus/crecimiento & desarrollo , Streptococcus/patogenicidadRESUMEN
A polymorphic CAG repeat was identified in the human alpha 1A voltage-dependent calcium channel subunit. To test the hypothesis that expansion of this CAG repeat could be the cause of an inherited progressive ataxia, we genotyped a large number of unrelated controls and ataxia patients. Eight unrelated patients with late onset ataxia had alleles with larger repeat numbers (21-27) compared to the number of repeats (4-16) in 475 non-ataxia individuals. Analysis of the repeat length in families of the affected individuals revealed that the expansion segregated with the phenotype in every patient. We identified six isoforms of the human alpha 1A calcium channel subunit. The CAG repeat is within the open reading frame and is predicted to encode glutamine in three of the isoforms. We conclude that a small polyglutamine expansion in the human alpha 1A calcium channel is most likely the cause of a newly classified autosomal dominant spinocerebellar ataxia, SCA6.
Asunto(s)
Canales de Calcio/genética , Ataxia Cerebelosa/genética , Genes Dominantes , Proteínas del Tejido Nervioso/genética , Péptidos/genética , Alelos , Secuencia de Aminoácidos , Animales , Ataxia Cerebelosa/patología , Femenino , Genotipo , Humanos , Masculino , Datos de Secuencia Molecular , Mutación , Linaje , Conejos , Homología de Secuencia de Aminoácido , Repeticiones de TrinucleótidosRESUMEN
Investigation of the vegetative nervous system (VNS) state according to ratio of the sympathetic and parasympathetic parts activity in patients with acute appendicitis, strangulated hernia, perforative ulcer was conducted. There were revealed significant VNS tone disorders before and after operation with signs of sympathicotony or parasimpathicotony, which needed to be corrected in complex of treatment of the patients.
Asunto(s)
Adaptación Fisiológica , Apendicitis/cirugía , Sistema Nervioso Autónomo/fisiología , Hernia Abdominal/cirugía , Úlcera Péptica Perforada/cirugía , Enfermedad Aguda , Adulto , Femenino , Humanos , Masculino , Persona de Mediana EdadRESUMEN
Bacteriological investigation of processus vermiformis contents was performed in 102 patients suffering an acute appendicitis (AA) and of abdominal cavity exudate--in 82 patients, operated for an AA. The microflora identification was done, as well as probable connection between microorganisms associations, the rate of the AA destructive forms revealing and postoperative purulent-inflammatory complications occurrence.
Asunto(s)
Apendicitis/microbiología , Infecciones Bacterianas/epidemiología , Infecciones Bacterianas/microbiología , Complicaciones Posoperatorias/epidemiología , Infección de la Herida Quirúrgica/epidemiología , Infección de la Herida Quirúrgica/microbiología , Enfermedad Aguda , HumanosRESUMEN
The expansion of polyglutamine tracts encoded by CAG trinucleotide repeats is a common mutational mechanism in inherited neurodegenerative diseases. Spinocerebellar ataxia type 6 (SCA6), an autosomal dominant, progressive disease, arises from trinucleotide repeat expansions present in the coding region of CACNA1A (chromosome 19p13). This gene encodes alpha(1A), the principal subunit of P/Q-type Ca(2+) channels, which are abundant in the CNS, particularly in cerebellar Purkinje and granule neurons. We assayed ion channel function by introduction of human alpha(1A) cDNAs in human embryonic kidney 293 cells that stably coexpressed beta(1) and alpha(2)delta subunits. Immunocytochemical analysis showed a rise in intracellular and surface expression of alpha(1A) protein when CAG repeat lengths reached or exceeded the pathogenic range for SCA6. This gain at the protein level was not a consequence of changes in RNA stability, as indicated by Northern blot analysis. The electrophysiological behavior of alpha(1A) subunits containing expanded (EXP) numbers of CAG repeats (23, 27, and 72) was compared against that of wild-type subunits (WT) (4 and 11 repeats) using standard whole-cell patch-clamp recording conditions. The EXP alpha(1A) subunits yielded functional ion channels that supported inward Ca(2+) channel currents, with a sharp increase in P/Q Ca(2+) channel current density relative to WT. Our results showed that Ca(2+) channels from SCA6 patients display near-normal biophysical properties but increased current density attributable to elevated protein expression at the cell surface.
Asunto(s)
Canales de Calcio/genética , Canales de Calcio/metabolismo , Subunidades de Proteína , Ataxias Espinocerebelosas/etiología , Expansión de Repetición de Trinucleótido/genética , Northern Blotting , Calcio/metabolismo , Canales de Calcio Tipo P/genética , Canales de Calcio Tipo P/metabolismo , Canales de Calcio Tipo Q/genética , Canales de Calcio Tipo Q/metabolismo , Línea Celular , Membrana Celular/metabolismo , Cromosomas Humanos Par 19/genética , ADN Complementario/genética , ADN Complementario/metabolismo , Expresión Génica , Genes Dominantes , Humanos , Inmunohistoquímica , Transporte Iónico/genética , Riñón/citología , Riñón/metabolismo , Técnicas de Placa-Clamp , ARN Mensajero/metabolismo , Ataxias Espinocerebelosas/metabolismo , TransfecciónRESUMEN
Individual mRNA coding for proteins of the alpha-polymerase complex were isolated from replicating hepatocytes. cDNAs were synthesized by reverse transcriptase. Three clones were identified by colony hybridization of the S period cDNA library. The sequences of these clones were complementary to the investigated mRNAs. Two clones named pr12 and pr167 revealed increased expressions in the S period. The level of mRNA pr127 does not change during liver regeneration. The amount of pr127 mRNA was about 1% of the total mRNA population. pr12, pr127 and pr167 mRNAs were isolated by the hybrid-selection method and were translated in a cell-free system. The products of translation were analysed by "activity" gel. It was shown that pr167 mRNA coded for protein 140 kDa with DNA polymerase activity. pr12 protein is an unknown component of the alpha-polymerase complex. We suggested that this protein participates in the initiation of DNA replication.
Asunto(s)
ADN Polimerasa II/genética , Replicación del ADN , Expresión Génica , Hígado/metabolismo , Animales , ADN/genética , ADN Polimerasa II/metabolismo , Immunoblotting , Hígado/citología , Hígado/enzimología , Hibridación de Ácido Nucleico , Plásmidos , Biosíntesis de Proteínas , ARN Mensajero/genética , RatasRESUMEN
Experiments on poly(U)-dependent binding of Phe-tRNAPhe to 30S subunits revealed the existence of a critical [Mg2+]/[NH4+] ratio in a medium (approximately 0.05-0.1) with respect to the binding capacity of subunits. If the ratio is greater than the critical one, 30S subunits undergo reversible inactivation even at the highest Mg2+ concentrations (up to 20 mM). The stronger is the deviation from the [Mg2+]/[NH4+] value = 0.05-0.1, the greater are both the rate and extent of such an inactivation. Two sites for tRNA in initially active 30S subunits have been shown to be inactivated in an interdependent way. On the other hand, a progressive decrease of [Mg2+]/[NH4+] ratio in a medium (from the value of 0.05 and lower) does not produce inactivation, but rather results in reduced affinity constants of Phe-tRNAPhe for active sites of 30S subunits.
Asunto(s)
Cationes Bivalentes/farmacología , Cationes Monovalentes/farmacología , Escherichia coli/metabolismo , Proteínas Ribosómicas/metabolismo , Ribosomas/metabolismo , Relación Dosis-Respuesta a Droga , Escherichia coli/efectos de los fármacos , Calor , Cinética , Aminoacil-ARN de Transferencia/biosíntesis , Ribosomas/efectos de los fármacosRESUMEN
Three major polypeptides are found in purified DNA polymerase alpha from rat liver: 160, 77 and 58 kDa. The electrophoretic analysis has identified polypeptide 160 kDa as the catalytically active subunit of DNA polymerase alpha. The other two polypeptides showed no DNA polymerase activity. Individual polypeptide p77 kDa purified by sodium dodecyl sulfate polyacrylamide gel electrophoresis was used to produce antibodies in rabbits. Immunoblot analysis indicated that the complex DNA polymerase alpha-3'-5'-exonuclease contained polypeptide p77 kDa. To elucidate the function of the p77 kDa protein we have prepared an immunoabsorbent column with antibodies against the p77 kDa polypeptide. The antibody column purified p77 kDa protein was homogeneous according to sodium dodecyl sulfate gel electrophoresis. The activity of alpha-polymerase was increased approximately 10-fold as a result of purification of DNA polymerase alpha from the p77 kDa protein. The in vitro experiments showed the identity of the p77 kDa polypeptide to endonuclease. It cleaved both single-stranded and double-stranded DNA. The function of endonuclease p77 kDA in complex with DNA polymerase alpha remains obscure.
Asunto(s)
ADN Polimerasa II/aislamiento & purificación , Endonucleasas/aislamiento & purificación , Hígado/enzimología , Animales , ADN Polimerasa II/metabolismo , Electroforesis en Gel de Poliacrilamida , Endonucleasas/metabolismo , Peso Molecular , Complejos Multienzimáticos/análisis , Péptidos/análisis , RatasRESUMEN
A library of double-stranded cDNA was prepared using poly (A) + RNA from regenerating rat liver 20 h after partial hepatectomy. Differential screening of 350 recombinant clones with cDNA-G0 and cDNA-S identified eleven cDNA clones (pRL), the sequences of which were preferentially expressed during the DNA replication period. Levels of mRNAs complementary to these clones were 2--10-fold higher in the S-period, than in G0. Using plasmid cDNAs to different mRNA, pRL we have investigated the changes in the levels of mRNA pRL during liver regeneration. The level of mRNA mRL2 and pRL79 was increased just before DNA replication. mRNA pRL35 accumulates after partial hepatectomy with the maximum at 6 h. The augment of two other mRNA concentrations was expressed to a lesser extent. Northern-blot analysis allowed to determine the individual dual mRNAs corresponding to each of the three clones with their sizes ranging from about 1650 to 3900 bases. Three mRNAs (pRL35, 67 and 79) were shown (by hybrid-selected translation) to code for proteins of about 100, 140 and 120 kDa, respectively.
Asunto(s)
Replicación del ADN , ADN/genética , ARN Mensajero/aislamiento & purificación , Animales , Secuencia de Bases , Hígado/análisis , Hibridación de Ácido Nucleico , Biosíntesis de Proteínas , ARN Mensajero/genética , Conejos , Ratas , Moldes GenéticosRESUMEN
The complex of interrelated pathogenic factors in development of postoperative suppurative inflammatory complication with abdominal cavity organs lesion was studied on the example of an acute appendicitis. It includes enteropathogenic microorganisms, insufficiency of local immunity, deficiency of immunoglobulins, lysozym. Findings show the possibility in seeking the measures of prevention of emergent state, in particular, an acute appendicitis.
Asunto(s)
Apendicitis/cirugía , Infecciones Bacterianas/etiología , Candidiasis/etiología , Inmunidad Innata/inmunología , Peritonitis/etiología , Enfermedad Aguda , Apendicectomía , Apendicitis/complicaciones , Apendicitis/inmunología , Apendicitis/microbiología , Apéndice/microbiología , Apéndice/cirugía , Infecciones Bacterianas/inmunología , Infecciones Bacterianas/microbiología , Candidiasis/inmunología , Candidiasis/microbiología , Humanos , Inmunoglobulinas/sangre , Cavidad Peritoneal/microbiología , Peritonitis/inmunología , Peritonitis/microbiologíaRESUMEN
New technology of the operative wound suturing was elaborated with performance of muscle-aponeurotic suture in 115 patients, operated on for emergent diseases, securing lowering of postoperative compications rate from 2.43 to 1.73%.
Asunto(s)
Servicios Médicos de Urgencia , Complicaciones Posoperatorias/prevención & control , Dehiscencia de la Herida Operatoria/etiología , Infección de la Herida Quirúrgica/etiología , Técnicas de Sutura/instrumentación , Abdomen , HumanosRESUMEN
One hundred and thirty six women with genital tuberculosis received combined antibacterial treatment (ABT) with adjuvant physiotherapy. The patients were divided into three groups: patients of group 1 had active genital tuberculosis treated with ABT plus electrophoresis of 3% sodium thiosulphate, group 2 had chronic tuberculous process treated with ABT plus ultrasound hydrocortisone therapy, group 3 received ABT alone. The findings demonstrate that noticeable improvement and reduction of treatment duration were observed in groups 1 and 2.
Asunto(s)
Antiinflamatorios/uso terapéutico , Antioxidantes/uso terapéutico , Antituberculosos/uso terapéutico , Hidrocortisona/uso terapéutico , Tiosulfatos/uso terapéutico , Tuberculosis de los Genitales Femeninos/terapia , Antiinflamatorios/administración & dosificación , Antioxidantes/administración & dosificación , Antituberculosos/administración & dosificación , Electroforesis , Endometrio/efectos de los fármacos , Endometrio/patología , Femenino , Humanos , Hidrocortisona/administración & dosificación , Fonoforesis , Tiosulfatos/administración & dosificaciónAsunto(s)
Conducta Anticonceptiva/estadística & datos numéricos , Anticonceptivos Hormonales Orales/administración & dosificación , Tuberculosis Pulmonar/epidemiología , Antituberculosos/farmacología , Antituberculosos/uso terapéutico , Anticonceptivos Hormonales Orales/metabolismo , Interacciones Farmacológicas , Femenino , Humanos , Tuberculosis Pulmonar/tratamiento farmacológicoAsunto(s)
Reproducción , Tuberculosis de los Genitales Femeninos/fisiopatología , Tuberculosis Pulmonar/fisiopatología , Tuberculosis Renal/fisiopatología , Adolescente , Adulto , Niño , Endometrio/diagnóstico por imagen , Femenino , Homeostasis , Humanos , Histerosalpingografía , Infertilidad Femenina/etiología , Infertilidad Femenina/terapia , Menarquia , Menstruación , Embarazo , Tuberculosis de los Genitales Femeninos/terapia , Tuberculosis Pulmonar/terapia , Tuberculosis Renal/terapia , Ultrasonografía , Útero/diagnóstico por imagenRESUMEN
The paper presents the specific features of diagnosis and clinical course of genital tuberculosis under the present conditions. It shows it expedient to make a differential diagnosis of genital tuberculosis in 2 steps: 1) the use of the routine examination and 2) the study of specific antituberculous immunity. Genital tuberculosis risk groups to be obligatorily examined at a specialized tuberculosis control facility are identified. The specific features of the clinical course of genital tuberculosis, such as aptness to exudative processes, a combination with extragenital tuberculosis and hormonally depended gynecological diseases are noted. Indications for and efficiency of endosurgical interventions in genital tuberculosis are shown.
Asunto(s)
Tuberculosis de los Genitales Femeninos/cirugía , Tuberculosis de los Genitales Masculinos/cirugía , Femenino , Humanos , MasculinoRESUMEN
Reciprocal probing has been used to identify a cDNA clone (xh8H11) representing a gene preferentially expressed in striated muscle. The gene maps close to DXS7101 31.9 cM from the short arm telomere of the X-chromosome at Xp22.1. On searching expressed and genomic databases, 21 expressed sequence tags were found that allowed the assignment of a human extended consensus sequence of 887 bp, suggesting a completely expressed gene symbolized as SMPX. By using the human consensus sequence, the orthologous mouse Smpx and rat SMPX genes could be aligned and confirmed by complete sequencing of additional SMPX-related clones obtained by library screening. An open reading frame was identified encoding a peptide of 88-86 and 85 amino acids in human and rodents, respectively. The predicted peptide had no significant homologies to known structural elements. The human consensus cDNA sequence was used to define the genomic structure of the human SMPX that had been missed by a previous large scale sequencing approach. The gene consists of five exons (> or =172, 57, 84, 148, > or =422 bp) and four introns (3639, 10410, 6052, 31134 bp) comprising together 52.1 kb and is preferentially and abundantly expressed in heart and skeletal muscle. Thus, a novel human gene encoding a small muscular protein that maps to Xp22.1 (SMPX) has been identified and structurally characterized as a basis for further functional analysis.
Asunto(s)
Proteínas Musculares/genética , Cromosoma X/genética , Secuencia de Aminoácidos , Animales , Secuencia de Bases , Mapeo Cromosómico , Clonación Molecular , Secuencia de Consenso , Cartilla de ADN/genética , ADN Complementario/genética , Etiquetas de Secuencia Expresada , Femenino , Humanos , Masculino , Ratones , Datos de Secuencia Molecular , Embarazo , Ratas , Homología de Secuencia de Aminoácido , Especificidad de la Especie , Distribución TisularRESUMEN
We report here the isolation, mapping, and genomic organization of the human NDUFA1 gene, which is a component of NADH:ubiquinone oxidoreductase (complex I). The NDUFA1 cDNA clone and associated genomic cosmid clones were isolated by reciprocal probing of an arrayed human heart cDNA library with a X-chromosome cosmid library and were mapped to Xq24. The NDUFA1 gene, which is highly expressed in human cardiac and skeletal muscle, has an open reading frame of 70 amino acids and shows 80% homology to the bovine MWFE subunit of complex I. By primer extension, the major and minor transcription initiation sites were identified, 99 and 141 nucleotides upstream of the translation initiator ATG, respectively. The NDUFA1 gene is composed of 3 exons and spans about 5.0 kb of genomic DNA. The 5' region of the NDUFA1 gene (approximately 450-bp fragment) lacks conventional TATA and CAAT boxes, but it contains several potential binding sites for transcription factors including SP-1, AP-2, NF1, NRF2-like, APRRE, CRE, MyoD1, CArG, MEF-2, and BRE.